| 6 |
3.166e-05 |
0.01761 |
bsu12160 |
yjgC |
-18.77 |
0.004394 |
190 -- 206 |
10 -- 27 |
oxidoreductase |
bsu27220 bsu03310 |
_____________189________________207\n_____________|__________________|\n5'-UAA...AGAAA__________________GGCAA...UGC-3'\n______________GGGGAAUGGAGA_UGGCU\n______________||||||::||||_|:|||\n______________CCCCUUGUCUCU_AUCGA\n3'-UUU...CCCAA____________C_____AGAGA...AUA-5'\n_____________|__________________|\n_____________28_________________9\n |
192 -- 198 |
19 -- 25 |
-27.0798 kcal/mol |
2.28534 kcal/mol |
6.0284 kcal/mol |
189 207@LB@ | |@LB@5'-UAA...AGAAA GGCAA...UGC-3'@LB@ GGGGAAUGGAGA UGGCU@LB@ ||||||::|||| |:|||@LB@ CCCCUUGUCUCU AUCGA@LB@3'-UUU...CCCAA C AGAGA...AUA-5'@LB@ | |@LB@ 28 9@LB@@LB@@LB@Energy -18.77 kcal/mol@LB@Hybridization Energy -27.0798 kcal/mol@LB@Unfolding Energy - mRNA 2.28534 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 190 -- 206@LB@Position - sRNA 10 -- 27@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 82 |
0.006245 |
0.254 |
bsu12400 |
yjnA |
-14.90 |
0.031572 |
235 -- 273 |
19 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________234_____________________________________274\n_____________|_______________________________________|\n5'-UUA...GUUUG_____GC___A___G___U__C___A__G__U_______CCUUA...UGC-3'\n______________UCGGA__CUU_GUC_GAU_GA_GGG_GU_GG_GGAGCGG\n______________|||||__|||_:||_|||_||_|||_||_||_|||:|:|\n______________AGCCU__GAA_UAG_CUA_CU_CCC_CA_CC_CCUUGUC\n3'-UUU...AAAAA_____A____C___A___U_________A__________UCUCA...AUA-5'\n_____________|_______________________________________|\n_____________54______________________________________18\n |
267 -- 273 |
19 -- 25 |
-33.8463 kcal/mol |
12.0212 kcal/mol |
6.92635 kcal/mol |
234 274@LB@ | |@LB@5'-UUA...GUUUG GC A G U C A G U CCUUA...UGC-3'@LB@ UCGGA CUU GUC GAU GA GGG GU GG GGAGCGG@LB@ ||||| ||| :|| ||| || ||| || || |||:|:|@LB@ AGCCU GAA UAG CUA CU CCC CA CC CCUUGUC@LB@3'-UUU...AAAAA A C A U A UCUCA...AUA-5'@LB@ | |@LB@ 54 18@LB@@LB@@LB@Energy -14.90 kcal/mol@LB@Hybridization Energy -33.8463 kcal/mol@LB@Unfolding Energy - mRNA 12.0212 kcal/mol@LB@Unfolding Energy - sRNA 6.92635 kcal/mol@LB@@LB@Position - mRNA 235 -- 273@LB@Position - sRNA 19 -- 53@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 49 |
0.002227 |
0.1516 |
bsu11839 |
yjzE |
-19.04 |
0.003744 |
170 -- 194 |
25 -- 51 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 11976315 |
|
_____________169_________________________195\n_____________|___________________________|\n5'-UCC...UUAAG___AAA___GACAGC____________UAUUG...GCU-3'\n______________GGA____GA______AGAGGGGU_GGG\n______________|||____||______||||||||_|||\n______________CCU____CU______UCUCCCCA_CCC\n3'-UUU...AAAAG___AGAA__AGACUA________A___CUUGU...AUA-5'\n_____________|___________________________|\n_____________52__________________________24\n |
185 -- 191 |
29 -- 35 |
-23.53 kcal/mol |
3.51134 kcal/mol |
0.976067 kcal/mol |
169 195@LB@ | |@LB@5'-UCC...UUAAG AAA GACAGC UAUUG...GCU-3'@LB@ GGA GA AGAGGGGU GGG@LB@ ||| || |||||||| |||@LB@ CCU CU UCUCCCCA CCC@LB@3'-UUU...AAAAG AGAA AGACUA A CUUGU...AUA-5'@LB@ | |@LB@ 52 24@LB@@LB@@LB@Energy -19.04 kcal/mol@LB@Hybridization Energy -23.53 kcal/mol@LB@Unfolding Energy - mRNA 3.51134 kcal/mol@LB@Unfolding Energy - sRNA 0.976067 kcal/mol@LB@@LB@Position - mRNA 170 -- 194@LB@Position - sRNA 25 -- 51@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 170 |
0.0164 |
0.3152 |
bsu13090 |
ykkC |
-8.27 |
0.358617 |
162 -- 212 |
23 -- 55 |
multidrug resistance protein |
|
_____________161__________________________________________________213\n_____________|____________________________________________________|\n5'-UCA...UUUGU_______GAA____A__A_______AA__AAUGACUCAAAAU_AAA______UUGGU...CCA-3'\n______________UUUCGGA___UCUU_AU__GAUAGA__GG_____________G___UGGGGA\n______________|||||||___||||_||__||||||__||_____________|___||||||\n______________AAAGCCU___AGAA_UA__CUAUCU__CC_____________C___ACCCCU\n3'-UUU...UCAAA______________C__GA______C_________________A________UGUCU...AUA-5'\n_____________|____________________________________________________|\n_____________56___________________________________________________22\n |
162 -- 168 |
49 -- 55 |
-21.902 kcal/mol |
11.1231 kcal/mol |
2.51158 kcal/mol |
161 213@LB@ | |@LB@5'-UCA...UUUGU GAA A A AA AAUGACUCAAAAU AAA UUGGU...CCA-3'@LB@ UUUCGGA UCUU AU GAUAGA GG G UGGGGA@LB@ ||||||| |||| || |||||| || | ||||||@LB@ AAAGCCU AGAA UA CUAUCU CC C ACCCCU@LB@3'-UUU...UCAAA C GA C A UGUCU...AUA-5'@LB@ | |@LB@ 56 22@LB@@LB@@LB@Energy -8.27 kcal/mol@LB@Hybridization Energy -21.902 kcal/mol@LB@Unfolding Energy - mRNA 11.1231 kcal/mol@LB@Unfolding Energy - sRNA 2.51158 kcal/mol@LB@@LB@Position - mRNA 162 -- 212@LB@Position - sRNA 23 -- 55@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 168 |
0.01631 |
0.3152 |
bsu14170 |
ykuP |
-12.43 |
0.089417 |
80 -- 100 |
11 -- 27 |
flavodoxin-2 |
|
_____________79____________________101\n_____________|_____________________|\n5'-CAC...AUUAA___ACAU______________CAAUC...UAG-3'\n______________GGG____GGAUGGAGAGUGGC\n______________|||____|:|::||||||:||\n______________CCC____CUUGUCUCUCAUCG\n3'-UUU...CCCAA_____________________AAGAG...AUA-5'\n_____________|_____________________|\n_____________28____________________10\n |
94 -- 100 |
11 -- 17 |
-24.7716 kcal/mol |
6.31596 kcal/mol |
6.0284 kcal/mol |
79 101@LB@ | |@LB@5'-CAC...AUUAA ACAU CAAUC...UAG-3'@LB@ GGG GGAUGGAGAGUGGC@LB@ ||| |:|::||||||:||@LB@ CCC CUUGUCUCUCAUCG@LB@3'-UUU...CCCAA AAGAG...AUA-5'@LB@ | |@LB@ 28 10@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -24.7716 kcal/mol@LB@Unfolding Energy - mRNA 6.31596 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 80 -- 100@LB@Position - sRNA 11 -- 27@LB@Position Seed - mRNA 94 -- 100@LB@Position Seed - sRNA 11 -- 17@LB@ |
| 192 |
0.01921 |
0.3269 |
bsu12649 |
ykzM |
-11.69 |
0.118448 |
82 -- 103 |
11 -- 32 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu26089 |
_____________81______________________104\n_____________|_______________________|\n5'-AAG...GACAA____A____________C_A___GGCCA...UCG-3'\n______________GGGU__GGGGACAGGGG_G_GGC\n______________||||__|||:||||:|:_|_:||\n______________CCCA__CCCUUGUCUCU_C_UCG\n3'-UUU...AUCUC____AC_____________A___AAGAG...AUA-5'\n_____________|_______________________|\n_____________33______________________10\n |
88 -- 94 |
19 -- 25 |
-26.088 kcal/mol |
7.89329 kcal/mol |
6.50677 kcal/mol |
81 104@LB@ | |@LB@5'-AAG...GACAA A C A GGCCA...UCG-3'@LB@ GGGU GGGGACAGGGG G GGC@LB@ |||| |||:||||:|: | :||@LB@ CCCA CCCUUGUCUCU C UCG@LB@3'-UUU...AUCUC AC A AAGAG...AUA-5'@LB@ | |@LB@ 33 10@LB@@LB@@LB@Energy -11.69 kcal/mol@LB@Hybridization Energy -26.088 kcal/mol@LB@Unfolding Energy - mRNA 7.89329 kcal/mol@LB@Unfolding Energy - sRNA 6.50677 kcal/mol@LB@@LB@Position - mRNA 82 -- 103@LB@Position - sRNA 11 -- 32@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 33 |
0.001178 |
0.119 |
bsu14840 |
ylaN |
-15.06 |
0.029338 |
181 -- 208 |
8 -- 28 |
depletion of this protein in Bacillus subtilis results in defects in cell morphology; crystal structure of Staphylococcus protein shows homodimer; ligand binding protein |
|
_____________180__________________________209\n_____________|____________________________|\n5'-GAU...UCGCC______________AUACUUU_______AGAGA...AUA-3'\n______________UGGGGAACGGAGGG_______UGGCUUC\n______________||||||||:|||:|_______|:|||||\n______________ACCCCUUGUCUCUC_______AUCGAAG\n3'-UUU...CCCCA____________________________AGAGAUA-5'\n_____________|____________________________|\n_____________29___________________________7\n |
202 -- 208 |
8 -- 14 |
-32.8109 kcal/mol |
11.1547 kcal/mol |
6.59627 kcal/mol |
180 209@LB@ | |@LB@5'-GAU...UCGCC AUACUUU AGAGA...AUA-3'@LB@ UGGGGAACGGAGGG UGGCUUC@LB@ ||||||||:|||:| |:|||||@LB@ ACCCCUUGUCUCUC AUCGAAG@LB@3'-UUU...CCCCA AGAGAUA-5'@LB@ | |@LB@ 29 7@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -32.8109 kcal/mol@LB@Unfolding Energy - mRNA 11.1547 kcal/mol@LB@Unfolding Energy - sRNA 6.59627 kcal/mol@LB@@LB@Position - mRNA 181 -- 208@LB@Position - sRNA 8 -- 28@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 8 -- 14@LB@ |
| 44 |
0.001968 |
0.1492 |
bsu14940 |
ylbA |
-14.48 |
0.038144 |
221 -- 240 |
24 -- 52 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________220___________________________241\n_____________|_____________________________|\n5'-CAU...GAAAG_________________________AA__CUGAG...AAU-3'\n______________CGGA_CUUGA________AGGGGUU__GG\n______________||||_|||||________|||||||__||\n______________GCCU_GAACU________UCCCCAA__CC\n3'-UUU...AAAAA____A_____AGACUAUC_______CC__UUGUC...AUA-5'\n_____________|_____________________________|\n_____________53____________________________23\n |
230 -- 236 |
28 -- 34 |
-22.4266 kcal/mol |
6.96942 kcal/mol |
0.980851 kcal/mol |
220 241@LB@ | |@LB@5'-CAU...GAAAG AA CUGAG...AAU-3'@LB@ CGGA CUUGA AGGGGUU GG@LB@ |||| ||||| ||||||| ||@LB@ GCCU GAACU UCCCCAA CC@LB@3'-UUU...AAAAA A AGACUAUC CC UUGUC...AUA-5'@LB@ | |@LB@ 53 23@LB@@LB@@LB@Energy -14.48 kcal/mol@LB@Hybridization Energy -22.4266 kcal/mol@LB@Unfolding Energy - mRNA 6.96942 kcal/mol@LB@Unfolding Energy - sRNA 0.980851 kcal/mol@LB@@LB@Position - mRNA 221 -- 240@LB@Position - sRNA 24 -- 52@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 86 |
0.006791 |
0.2588 |
bsu17010 |
ymcB |
-10.99 |
0.152529 |
169 -- 190 |
33 -- 52 |
(dimethylallyl)adenosine tRNA methylthiotransferase MiaB |
|
_____________168____________________191\n_____________|______________________|\n5'-AUC...AAGGG____AG__________AA____AAAAU...CGC-3'\n______________UGGA__UUUGGUUUGA__GGAG\n______________:|||__:|||:|:|||__:|||\n______________GCCU__GAACUAGACU__UCUC\n3'-UUU...AAAAA____A___________A_____CCCAA...AUA-5'\n_____________|______________________|\n_____________53_____________________32\n |
178 -- 184 |
38 -- 44 |
-16.81 kcal/mol |
4.84464 kcal/mol |
0.976367 kcal/mol |
168 191@LB@ | |@LB@5'-AUC...AAGGG AG AA AAAAU...CGC-3'@LB@ UGGA UUUGGUUUGA GGAG@LB@ :||| :|||:|:||| :|||@LB@ GCCU GAACUAGACU UCUC@LB@3'-UUU...AAAAA A A CCCAA...AUA-5'@LB@ | |@LB@ 53 32@LB@@LB@@LB@Energy -10.99 kcal/mol@LB@Hybridization Energy -16.81 kcal/mol@LB@Unfolding Energy - mRNA 4.84464 kcal/mol@LB@Unfolding Energy - sRNA 0.976367 kcal/mol@LB@@LB@Position - mRNA 169 -- 190@LB@Position - sRNA 33 -- 52@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 138 |
0.01215 |
0.2883 |
bsu17940 |
yneI |
-15.06 |
0.029368 |
176 -- 197 |
30 -- 52 |
plasmid maintenance protein CcdB |
|
_____________175_____________________198\n_____________|_______________________|\n5'-GAA...AUCUG____AA_C____GU_________AAUAU...CGG-3'\n______________UGGA__U_GAUU___UGGAGGGG\n______________:|||__|_|||:___|:||||||\n______________GCCU__A_CUAG___AUCUCCCC\n3'-UUU...AAAAA____AG_A____ACU________AACCC...AUA-5'\n_____________|_______________________|\n_____________53______________________29\n |
191 -- 197 |
30 -- 36 |
-21.3361 kcal/mol |
5.29839 kcal/mol |
0.979974 kcal/mol |
175 198@LB@ | |@LB@5'-GAA...AUCUG AA C GU AAUAU...CGG-3'@LB@ UGGA U GAUU UGGAGGGG@LB@ :||| | |||: |:||||||@LB@ GCCU A CUAG AUCUCCCC@LB@3'-UUU...AAAAA AG A ACU AACCC...AUA-5'@LB@ | |@LB@ 53 29@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -21.3361 kcal/mol@LB@Unfolding Energy - mRNA 5.29839 kcal/mol@LB@Unfolding Energy - sRNA 0.979974 kcal/mol@LB@@LB@Position - mRNA 176 -- 197@LB@Position - sRNA 30 -- 52@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 30 -- 36@LB@ |
