| 71 |
0.005061 |
0.2378 |
bsu02170 |
ybfB |
-12.87 |
0.075045 |
244 -- 269 |
24 -- 48 |
MFS transporter |
bsu03530 |
_____________243__________________________270\n_____________|____________________________|\n5'-CUU...CACCU_____________UU__C____CA____GUGCG...AGC-3'\n______________UUUUGAUCU__UA__GG_GGUU__GGGG\n______________|:|||||||__||__||_||||__||||\n______________AGAACUAGA__AU__CC_CCAA__CCCC\n3'-UUU...AGCCU_________CU__CU_____________UUGUC...AUA-5'\n_____________|____________________________|\n_____________49___________________________23\n |
246 -- 252 |
40 -- 46 |
-21.3597 kcal/mol |
7.5105 kcal/mol |
0.978761 kcal/mol |
243 270@LB@ | |@LB@5'-CUU...CACCU UU C CA GUGCG...AGC-3'@LB@ UUUUGAUCU UA GG GGUU GGGG@LB@ |:||||||| || || |||| ||||@LB@ AGAACUAGA AU CC CCAA CCCC@LB@3'-UUU...AGCCU CU CU UUGUC...AUA-5'@LB@ | |@LB@ 49 23@LB@@LB@@LB@Energy -12.87 kcal/mol@LB@Hybridization Energy -21.3597 kcal/mol@LB@Unfolding Energy - mRNA 7.5105 kcal/mol@LB@Unfolding Energy - sRNA 0.978761 kcal/mol@LB@@LB@Position - mRNA 244 -- 269@LB@Position - sRNA 24 -- 48@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 119 |
0.01041 |
0.2883 |
bsu11480 |
yjbB |
-12.30 |
0.094041 |
10 -- 19 |
22 -- 31 |
MFS transporter |
bsu07460 |
_____________9__________20\n_____________|__________|\n5'-UCA...UUAAA__A_______ACGAU...GGA-3'\n______________GG_UGGGGAA\n______________||_|||||||\n______________CC_ACCCCUU\n3'-UUU...UCUCC__A_______GUCUC...AUA-5'\n_____________|__________|\n_____________32_________21\n |
13 -- 19 |
22 -- 28 |
-13.9571 kcal/mol |
0.741777 kcal/mol |
0.918006 kcal/mol |
9 20@LB@ | |@LB@5'-UCA...UUAAA A ACGAU...GGA-3'@LB@ GG UGGGGAA@LB@ || |||||||@LB@ CC ACCCCUU@LB@3'-UUU...UCUCC A GUCUC...AUA-5'@LB@ | |@LB@ 32 21@LB@@LB@@LB@Energy -12.30 kcal/mol@LB@Hybridization Energy -13.9571 kcal/mol@LB@Unfolding Energy - mRNA 0.741777 kcal/mol@LB@Unfolding Energy - sRNA 0.918006 kcal/mol@LB@@LB@Position - mRNA 10 -- 19@LB@Position - sRNA 22 -- 31@LB@Position Seed - mRNA 13 -- 19@LB@Position Seed - sRNA 22 -- 28@LB@ |
| 190 |
0.01911 |
0.3269 |
bsu05370 |
ydfD |
-12.16 |
0.099313 |
269 -- 294 |
11 -- 36 |
GntR family transcriptional regulator |
bsu10880 |
_____________268________________________295\n_____________|__________________________|\n5'-AAA...AUUAA____AAAAUA__A_____________CGAUUG-3'\n______________AGAG______GG_AAUGGAGAGUGGC\n______________||||______||_||::||||||:||\n______________UCUC______CC_UUGUCUCUCAUCG\n3'-UUU...GACUA____CCCAAC__C_____________AAGAG...AUA-5'\n_____________|__________________________|\n_____________37_________________________10\n |
288 -- 294 |
11 -- 17 |
-24.9379 kcal/mol |
3.68177 kcal/mol |
9.10067 kcal/mol |
268 295@LB@ | |@LB@5'-AAA...AUUAA AAAAUA A CGAUUG-3'@LB@ AGAG GG AAUGGAGAGUGGC@LB@ |||| || ||::||||||:||@LB@ UCUC CC UUGUCUCUCAUCG@LB@3'-UUU...GACUA CCCAAC C AAGAG...AUA-5'@LB@ | |@LB@ 37 10@LB@@LB@@LB@Energy -12.16 kcal/mol@LB@Hybridization Energy -24.9379 kcal/mol@LB@Unfolding Energy - mRNA 3.68177 kcal/mol@LB@Unfolding Energy - sRNA 9.10067 kcal/mol@LB@@LB@Position - mRNA 269 -- 294@LB@Position - sRNA 11 -- 36@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 11 -- 17@LB@ |
| 79 |
0.005838 |
0.2448 |
bsu25980 |
yqbT |
-11.66 |
0.119794 |
52 -- 65 |
32 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type ps: structure' |
bsu12730 |
_____________51_____________________66\n_____________|______________________|\n5'-CGU...AUCCC______________________AAGCA...CGU-3'\n______________UCGG________CUGAUAGAGG\n______________||||________||||||||||\n______________AGCC________GACUAUCUCC\n3'-UUU...AAAAA____UAGAACUA__________CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
59 -- 65 |
32 -- 38 |
-19.4839 kcal/mol |
6.84464 kcal/mol |
0.981755 kcal/mol |
51 66@LB@ | |@LB@5'-CGU...AUCCC AAGCA...CGU-3'@LB@ UCGG CUGAUAGAGG@LB@ |||| ||||||||||@LB@ AGCC GACUAUCUCC@LB@3'-UUU...AAAAA UAGAACUA CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -19.4839 kcal/mol@LB@Unfolding Energy - mRNA 6.84464 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 52 -- 65@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 112 |
0.009326 |
0.2778 |
bsu13200 |
rsbRB |
-15.32 |
0.026068 |
265 -- 282 |
32 -- 51 |
RsbT co-antagonist protein RsbRB |
bsu19450 |
_____________264__________________283\n_____________|____________________|\n5'-CUU...GGAAU_________AAC________AAAGU...CGC-3'\n______________GGAU__UGA___GAUGGAGG\n______________||||__|||___|||:||||\n______________CCUA__ACU___CUAUCUCC\n3'-UUU...AAAAG____GA___AGA________CCAAC...AUA-5'\n_____________|____________________|\n_____________52___________________31\n |
276 -- 282 |
32 -- 38 |
-18.0013 kcal/mol |
1.71479 kcal/mol |
0.97021 kcal/mol |
264 283@LB@ | |@LB@5'-CUU...GGAAU AAC AAAGU...CGC-3'@LB@ GGAU UGA GAUGGAGG@LB@ |||| ||| |||:||||@LB@ CCUA ACU CUAUCUCC@LB@3'-UUU...AAAAG GA AGA CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -15.32 kcal/mol@LB@Hybridization Energy -18.0013 kcal/mol@LB@Unfolding Energy - mRNA 1.71479 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 265 -- 282@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 103 |
0.008788 |
0.2778 |
bsu06070 |
ydiP |
-8.66 |
0.321247 |
183 -- 194 |
27 -- 44 |
BsuMI modification methylase subunit YdiP |
bsu20250 |
_____________182________________195\n_____________|__________________|\n5'-GGA...AAAAA__________________AUAUC...UGG-3'\n______________GAUUUGA______GGUUG\n______________|||:|||______|||||\n______________CUAGACU______CCAAC\n3'-UUU...UAGAA_______AUCUCC_____CCCUU...AUA-5'\n_____________|__________________|\n_____________45_________________26\n |
183 -- 189 |
38 -- 44 |
-12.3729 kcal/mol |
2.73997 kcal/mol |
0.971452 kcal/mol |
182 195@LB@ | |@LB@5'-GGA...AAAAA AUAUC...UGG-3'@LB@ GAUUUGA GGUUG@LB@ |||:||| |||||@LB@ CUAGACU CCAAC@LB@3'-UUU...UAGAA AUCUCC CCCUU...AUA-5'@LB@ | |@LB@ 45 26@LB@@LB@@LB@Energy -8.66 kcal/mol@LB@Hybridization Energy -12.3729 kcal/mol@LB@Unfolding Energy - mRNA 2.73997 kcal/mol@LB@Unfolding Energy - sRNA 0.971452 kcal/mol@LB@@LB@Position - mRNA 183 -- 194@LB@Position - sRNA 27 -- 44@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 192 |
0.01921 |
0.3269 |
bsu12649 |
ykzM |
-11.69 |
0.118448 |
82 -- 103 |
11 -- 32 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu26089 |
_____________81______________________104\n_____________|_______________________|\n5'-AAG...GACAA____A____________C_A___GGCCA...UCG-3'\n______________GGGU__GGGGACAGGGG_G_GGC\n______________||||__|||:||||:|:_|_:||\n______________CCCA__CCCUUGUCUCU_C_UCG\n3'-UUU...AUCUC____AC_____________A___AAGAG...AUA-5'\n_____________|_______________________|\n_____________33______________________10\n |
88 -- 94 |
19 -- 25 |
-26.088 kcal/mol |
7.89329 kcal/mol |
6.50677 kcal/mol |
81 104@LB@ | |@LB@5'-AAG...GACAA A C A GGCCA...UCG-3'@LB@ GGGU GGGGACAGGGG G GGC@LB@ |||| |||:||||:|: | :||@LB@ CCCA CCCUUGUCUCU C UCG@LB@3'-UUU...AUCUC AC A AAGAG...AUA-5'@LB@ | |@LB@ 33 10@LB@@LB@@LB@Energy -11.69 kcal/mol@LB@Hybridization Energy -26.088 kcal/mol@LB@Unfolding Energy - mRNA 7.89329 kcal/mol@LB@Unfolding Energy - sRNA 6.50677 kcal/mol@LB@@LB@Position - mRNA 82 -- 103@LB@Position - sRNA 11 -- 32@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 162 |
0.01461 |
0.3008 |
bsu28700 |
ysfE |
-18.20 |
0.006050 |
31 -- 59 |
26 -- 51 |
Evidence 5: No homology to any previously reported sequences |
bsu26940 |
_____________30____________________________60\n_____________|_____________________________|\n5'-AAG...UAGAA___CUA____AAA__CUG___________CAAUG...AAA-3'\n______________GGA___UUGA___UG___AGAGGGGUUGG\n______________|||___||||___||___|||||||||||\n______________CCU___AACU___AC___UCUCCCCAACC\n3'-UUU...AAAAG___AG_____AG___UA____________CCUUG...AUA-5'\n_____________|_____________________________|\n_____________52____________________________25\n |
53 -- 59 |
26 -- 32 |
-27.3955 kcal/mol |
8.22133 kcal/mol |
0.975822 kcal/mol |
30 60@LB@ | |@LB@5'-AAG...UAGAA CUA AAA CUG CAAUG...AAA-3'@LB@ GGA UUGA UG AGAGGGGUUGG@LB@ ||| |||| || |||||||||||@LB@ CCU AACU AC UCUCCCCAACC@LB@3'-UUU...AAAAG AG AG UA CCUUG...AUA-5'@LB@ | |@LB@ 52 25@LB@@LB@@LB@Energy -18.20 kcal/mol@LB@Hybridization Energy -27.3955 kcal/mol@LB@Unfolding Energy - mRNA 8.22133 kcal/mol@LB@Unfolding Energy - sRNA 0.975822 kcal/mol@LB@@LB@Position - mRNA 31 -- 59@LB@Position - sRNA 26 -- 51@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 26 -- 32@LB@ |
| 6 |
3.166e-05 |
0.01761 |
bsu12160 |
yjgC |
-18.77 |
0.004394 |
190 -- 206 |
10 -- 27 |
oxidoreductase |
bsu27220 bsu03310 |
_____________189________________207\n_____________|__________________|\n5'-UAA...AGAAA__________________GGCAA...UGC-3'\n______________GGGGAAUGGAGA_UGGCU\n______________||||||::||||_|:|||\n______________CCCCUUGUCUCU_AUCGA\n3'-UUU...CCCAA____________C_____AGAGA...AUA-5'\n_____________|__________________|\n_____________28_________________9\n |
192 -- 198 |
19 -- 25 |
-27.0798 kcal/mol |
2.28534 kcal/mol |
6.0284 kcal/mol |
189 207@LB@ | |@LB@5'-UAA...AGAAA GGCAA...UGC-3'@LB@ GGGGAAUGGAGA UGGCU@LB@ ||||||::|||| |:|||@LB@ CCCCUUGUCUCU AUCGA@LB@3'-UUU...CCCAA C AGAGA...AUA-5'@LB@ | |@LB@ 28 9@LB@@LB@@LB@Energy -18.77 kcal/mol@LB@Hybridization Energy -27.0798 kcal/mol@LB@Unfolding Energy - mRNA 2.28534 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 190 -- 206@LB@Position - sRNA 10 -- 27@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 1 |
3.729e-08 |
0.0001244 |
bsu14890 |
ctaC |
-17.27 |
0.009980 |
187 -- 199 |
23 -- 34 |
cytochrome c oxidase subunit 2 |
|
_____________186___________200\n_____________|_____________|\n5'-CGA...AGAAA__________U__AAUGG...GCG-3'\n______________AGGGGUUGGG_GA\n______________||||||||||_||\n______________UCCCCAACCC_CU\n3'-UUU...CUAUC_____________UGUCU...AUA-5'\n_____________|_____________|\n_____________35____________22\n |
190 -- 196 |
25 -- 31 |
-21.5157 kcal/mol |
4.12609 kcal/mol |
0.123213 kcal/mol |
186 200@LB@ | |@LB@5'-CGA...AGAAA U AAUGG...GCG-3'@LB@ AGGGGUUGGG GA@LB@ |||||||||| ||@LB@ UCCCCAACCC CU@LB@3'-UUU...CUAUC UGUCU...AUA-5'@LB@ | |@LB@ 35 22@LB@@LB@@LB@Energy -17.27 kcal/mol@LB@Hybridization Energy -21.5157 kcal/mol@LB@Unfolding Energy - mRNA 4.12609 kcal/mol@LB@Unfolding Energy - sRNA 0.123213 kcal/mol@LB@@LB@Position - mRNA 187 -- 199@LB@Position - sRNA 23 -- 34@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 25 -- 31@LB@ |
