Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
75	0.01364	0.5385	b0107	hofB	-15.68	0.010425	272 -- 289	69 -- 87	T2SE secretion family protein; P-loop ATPase superfamily protein	_____________271_________________290\n_____________\|___________________\|\n5'-CCA...GAGGU_____________GG____CGAUG...CUU-3'\n______________GGUUCAUGUUG_C__UAGU\n______________:\|\|\|\|\|:\|\|\|\|_\|__\|\|\|\|\n______________UCAAGUGCAAC_G__AUCA\n3'-CGC...CGUUU___________C_AA____AUAAG...AAG-5'\n_____________\|___________________\|\n_____________88__________________68\n	276 -- 282	77 -- 83	-18.3354 kcal/mol	2.08484 kcal/mol	0.569189 kcal/mol	271 290@LB@ \| \|@LB@5'-CCA...GAGGU GG CGAUG...CUU-3'@LB@ GGUUCAUGUUG C UAGU@LB@ :\|\|\|\|\|:\|\|\|\| \| \|\|\|\|@LB@ UCAAGUGCAAC G AUCA@LB@3'-CGC...CGUUU C AA AUAAG...AAG-5'@LB@ \| \|@LB@ 88 68@LB@@LB@@LB@Energy -15.68 kcal/mol@LB@Hybridization Energy -18.3354 kcal/mol@LB@Unfolding Energy - mRNA 2.08484 kcal/mol@LB@Unfolding Energy - sRNA 0.569189 kcal/mol@LB@@LB@Position - mRNA 272 -- 289@LB@Position - sRNA 69 -- 87@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 77 -- 83@LB@
98	0.0165	0.5385	b0151	fhuC	-12.15	0.044517	184 -- 202	66 -- 85	iron(3+)-hydroxamate import ABC transporter ATPase	_____________183____________________203\n_____________\|______________________\|\n5'-CUG...CCCAU_____AA_______________GCAGG...CCU-3'\n______________UUCAC__GUUGGCU___GUUAU\n______________\|\|\|\|\|__\|\|\|\|\|\|\|___\|\|\|\|\|\n______________AAGUG__CAACCGA___CAAUA\n3'-CGC...UUUUC______________AAU_____AGCUC...AAG-5'\n_____________\|______________________\|\n_____________86_____________________65\n	191 -- 197	74 -- 80	-16.912 kcal/mol	4.1925 kcal/mol	0.573112 kcal/mol	183 203@LB@ \| \|@LB@5'-CUG...CCCAU AA GCAGG...CCU-3'@LB@ UUCAC GUUGGCU GUUAU@LB@ \|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ AAGUG CAACCGA CAAUA@LB@3'-CGC...UUUUC AAU AGCUC...AAG-5'@LB@ \| \|@LB@ 86 65@LB@@LB@@LB@Energy -12.15 kcal/mol@LB@Hybridization Energy -16.912 kcal/mol@LB@Unfolding Energy - mRNA 4.1925 kcal/mol@LB@Unfolding Energy - sRNA 0.573112 kcal/mol@LB@@LB@Position - mRNA 184 -- 202@LB@Position - sRNA 66 -- 85@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 74 -- 80@LB@
31	0.005384	0.5385	b0159	mtn	-14.65	0.015942	9 -- 52	71 -- 108	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	_____________8____________________________________________53\n_____________\|____________________________________________\|\n___5'-GAUACGCC__C____G__CUC_A____________UC_______C_______ACGCC...UCU-3'\n______________CG_GAUC_CU___G_AGAUCCGCAGGA__UCAUGUU_GGUUUUA\n______________\|\|_\|\|\|\|_\|\|___\|_\|\|\|\|\|\|\|\|\|::\|__\|\|\|:\|\|\|_\|\|:\|\|\|\|\n______________GC_CUAG_GA___C_UCUAGGCGUUUU__AGUGCAA_CCGAAAU\n__________3'-C__________C________________CA_______________CAAUA...AAG-5'\n_____________\|____________________________________________\|\n_____________109__________________________________________70\n	46 -- 52	71 -- 77	-39.2779 kcal/mol	12.7007 kcal/mol	11.9275 kcal/mol	8 53@LB@ \| \|@LB@ 5'-GAUACGCC C G CUC A UC C ACGCC...UCU-3'@LB@ CG GAUC CU G AGAUCCGCAGGA UCAUGUU GGUUUUA@LB@ \|\| \|\|\|\| \|\| \| \|\|\|\|\|\|\|\|\|::\| \|\|\|:\|\|\| \|\|:\|\|\|\|@LB@ GC CUAG GA C UCUAGGCGUUUU AGUGCAA CCGAAAU@LB@ 3'-C C CA CAAUA...AAG-5'@LB@ \| \|@LB@ 109 70@LB@@LB@@LB@Energy -14.65 kcal/mol@LB@Hybridization Energy -39.2779 kcal/mol@LB@Unfolding Energy - mRNA 12.7007 kcal/mol@LB@Unfolding Energy - sRNA 11.9275 kcal/mol@LB@@LB@Position - mRNA 9 -- 52@LB@Position - sRNA 71 -- 108@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 71 -- 77@LB@
168	0.0298	0.5559	b0161	degP	-8.84	0.166335	239 -- 253	75 -- 88	serine endoprotease (protease Do) membrane-associated	_____________238_____________254\n_____________\|_______________\|\n5'-AUU...GCUCU_______G_U_____GUUAU...CGA-3'\n______________GAGUUUA_G_UUGGC\n______________:\|\|\|\|:\|_\|_\|\|\|\|\|\n______________UUCAAGU_C_AACCG\n3'-CGC...GCGUU_______G_______AAAUC...AAG-5'\n_____________\|_______________\|\n_____________89______________74\n	239 -- 245	82 -- 88	-14.9204 kcal/mol	5.56475 kcal/mol	0.515884 kcal/mol	238 254@LB@ \| \|@LB@5'-AUU...GCUCU G U GUUAU...CGA-3'@LB@ GAGUUUA G UUGGC@LB@ :\|\|\|\|:\| \| \|\|\|\|\|@LB@ UUCAAGU C AACCG@LB@3'-CGC...GCGUU G AAAUC...AAG-5'@LB@ \| \|@LB@ 89 74@LB@@LB@@LB@Energy -8.84 kcal/mol@LB@Hybridization Energy -14.9204 kcal/mol@LB@Unfolding Energy - mRNA 5.56475 kcal/mol@LB@Unfolding Energy - sRNA 0.515884 kcal/mol@LB@@LB@Position - mRNA 239 -- 253@LB@Position - sRNA 75 -- 88@LB@Position Seed - mRNA 239 -- 245@LB@Position Seed - sRNA 82 -- 88@LB@
69	0.01301	0.5385	b0174	ispU	-11.35	0.061456	97 -- 106	71 -- 80	undecaprenyl pyrophosphate synthase	_____________96_________107\n_____________\|__________\|\n5'-CGU...AAGUA__________UAAGG...AAA-3'\n______________GUUGGCUUUA\n______________\|\|\|\|\|\|\|\|\|\|\n______________CAACCGAAAU\n3'-CGC...AAGUG__________CAAUA...AAG-5'\n_____________\|__________\|\n_____________81_________70\n	100 -- 106	71 -- 77	-14.3232 kcal/mol	2.73321 kcal/mol	0.236872 kcal/mol	96 107@LB@ \| \|@LB@5'-CGU...AAGUA UAAGG...AAA-3'@LB@ GUUGGCUUUA@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ CAACCGAAAU@LB@3'-CGC...AAGUG CAAUA...AAG-5'@LB@ \| \|@LB@ 81 70@LB@@LB@@LB@Energy -11.35 kcal/mol@LB@Hybridization Energy -14.3232 kcal/mol@LB@Unfolding Energy - mRNA 2.73321 kcal/mol@LB@Unfolding Energy - sRNA 0.236872 kcal/mol@LB@@LB@Position - mRNA 97 -- 106@LB@Position - sRNA 71 -- 80@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 71 -- 77@LB@
133	0.02275	0.5385	b0177	bamA	-6.79	0.350358	265 -- 271	23 -- 29	BamABCDE complex OM biogenesis outer membrane pore-forming assembly factor	_____________264_____272\n_____________\|_______\|\n5'-CUG...UGCUG_______CGUAG...GCC-3'\n______________AAGGGUU\n______________\|\|\|\|\|\|\|\n______________UUCCCAA\n3'-CGC...UGAAG_______UUUUC...AAG-5'\n_____________\|_______\|\n_____________30______22\n	265 -- 271	23 -- 29	-7.93739 kcal/mol	1.09969 kcal/mol	0.0462613 kcal/mol	264 272@LB@ \| \|@LB@5'-CUG...UGCUG CGUAG...GCC-3'@LB@ AAGGGUU@LB@ \|\|\|\|\|\|\|@LB@ UUCCCAA@LB@3'-CGC...UGAAG UUUUC...AAG-5'@LB@ \| \|@LB@ 30 22@LB@@LB@@LB@Energy -6.79 kcal/mol@LB@Hybridization Energy -7.93739 kcal/mol@LB@Unfolding Energy - mRNA 1.09969 kcal/mol@LB@Unfolding Energy - sRNA 0.0462613 kcal/mol@LB@@LB@Position - mRNA 265 -- 271@LB@Position - sRNA 23 -- 29@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 23 -- 29@LB@
150	0.02548	0.5485	b0180	fabZ	-10.02	0.104939	240 -- 263	1 -- 24	(3R)-hydroxymyristol acyl carrier protein dehydratase	_____________239______________________264\n_____________\|________________________\|\n5'-AAA...AGAUU______CUUC______A_______CCGUU...AAG-3'\n______________UUAGAA____UGCCGC_CCGUUUC\n______________\|\|\|:\|\|____\|\|\|\|\|\|_\|\|\|\|\|\|\|\n______________AAUUUU____ACGGCG_GGCAAAG\n3'-CGC...UUCCC______CUCC______A_______-5'\n_____________\|_______________________\|\n_____________25______________________1\n	257 -- 263	1 -- 7	-23.7452 kcal/mol	3.39044 kcal/mol	10.3355 kcal/mol	239 264@LB@ \| \|@LB@5'-AAA...AGAUU CUUC A CCGUU...AAG-3'@LB@ UUAGAA UGCCGC CCGUUUC@LB@ \|\|\|:\|\| \|\|\|\|\|\| \|\|\|\|\|\|\|@LB@ AAUUUU ACGGCG GGCAAAG@LB@3'-CGC...UUCCC CUCC A -5'@LB@ \| \|@LB@ 25 1@LB@@LB@@LB@Energy -10.02 kcal/mol@LB@Hybridization Energy -23.7452 kcal/mol@LB@Unfolding Energy - mRNA 3.39044 kcal/mol@LB@Unfolding Energy - sRNA 10.3355 kcal/mol@LB@@LB@Position - mRNA 240 -- 263@LB@Position - sRNA 1 -- 24@LB@Position Seed - mRNA 257 -- 263@LB@Position Seed - sRNA 1 -- 7@LB@
124	0.02124	0.5385	b0198	metI	-9.85	0.112177	270 -- 285	71 -- 87	DL-methionine transporter subunit	_____________269_______________286\n_____________\|_________________\|\n5'-UCA...UAUCC_____UU__________UGAUU...CGG-3'\n______________GGUUU___UUGGCUUUG\n______________:\|\|\|:___\|\|\|\|\|\|\|\|:\n______________UCAAG___AACCGAAAU\n3'-CGC...CGUUU_____UGC_________CAAUA...AAG-5'\n_____________\|_________________\|\n_____________88________________70\n	279 -- 285	71 -- 77	-13.0228 kcal/mol	2.60961 kcal/mol	0.562376 kcal/mol	269 286@LB@ \| \|@LB@5'-UCA...UAUCC UU UGAUU...CGG-3'@LB@ GGUUU UUGGCUUUG@LB@ :\|\|\|: \|\|\|\|\|\|\|\|:@LB@ UCAAG AACCGAAAU@LB@3'-CGC...CGUUU UGC CAAUA...AAG-5'@LB@ \| \|@LB@ 88 70@LB@@LB@@LB@Energy -9.85 kcal/mol@LB@Hybridization Energy -13.0228 kcal/mol@LB@Unfolding Energy - mRNA 2.60961 kcal/mol@LB@Unfolding Energy - sRNA 0.562376 kcal/mol@LB@@LB@Position - mRNA 270 -- 285@LB@Position - sRNA 71 -- 87@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 71 -- 77@LB@
182	0.03197	0.5559	b0212	gloB	-7.96	0.231442	50 -- 71	56 -- 75	hydroxyacylglutathione hydrolase	_____________49_____________________72\n_____________\|______________________\|\n5'-UUG...AUACA_______GAACCA_________CUGGC...AGC-3'\n______________UUUUAGU______CGGGUUGAG\n______________:\|\|\|\|\|\|______\|\|:\|\|\|\|\|\|\n______________GAAAUCA______GCUCAACUC\n3'-CGC...CAACC_______AUAA___________UUUGA...AAG-5'\n_____________\|______________________\|\n_____________76_____________________55\n	65 -- 71	56 -- 62	-16.3693 kcal/mol	6.6953 kcal/mol	1.71204 kcal/mol	49 72@LB@ \| \|@LB@5'-UUG...AUACA GAACCA CUGGC...AGC-3'@LB@ UUUUAGU CGGGUUGAG@LB@ :\|\|\|\|\|\| \|\|:\|\|\|\|\|\|@LB@ GAAAUCA GCUCAACUC@LB@3'-CGC...CAACC AUAA UUUGA...AAG-5'@LB@ \| \|@LB@ 76 55@LB@@LB@@LB@Energy -7.96 kcal/mol@LB@Hybridization Energy -16.3693 kcal/mol@LB@Unfolding Energy - mRNA 6.6953 kcal/mol@LB@Unfolding Energy - sRNA 1.71204 kcal/mol@LB@@LB@Position - mRNA 50 -- 71@LB@Position - sRNA 56 -- 75@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 56 -- 62@LB@
199	0.03481	0.5559	b0407	yajC	-8.41	0.195839	88 -- 104	15 -- 30	SecYEG protein translocase auxillary subunit	_____________87________________105\n_____________\|_________________\|\n5'-UCU...UUACG_____CUAUU_______GUAAA...UUU-3'\n______________CAAGG_____GAAGAGG\n______________\|\|\|\|\|_____:\|\|\|\|\|\|\n______________GUUCC_____UUUCUCC\n3'-CGC...CUGAA_____CAAU________ACGGC...AAG-5'\n_____________\|_________________\|\n_____________31________________14\n	98 -- 104	15 -- 21	-15.1331 kcal/mol	3.4367 kcal/mol	3.28624 kcal/mol	87 105@LB@ \| \|@LB@5'-UCU...UUACG CUAUU GUAAA...UUU-3'@LB@ CAAGG GAAGAGG@LB@ \|\|\|\|\| :\|\|\|\|\|\|@LB@ GUUCC UUUCUCC@LB@3'-CGC...CUGAA CAAU ACGGC...AAG-5'@LB@ \| \|@LB@ 31 14@LB@@LB@@LB@Energy -8.41 kcal/mol@LB@Hybridization Energy -15.1331 kcal/mol@LB@Unfolding Energy - mRNA 3.4367 kcal/mol@LB@Unfolding Energy - sRNA 3.28624 kcal/mol@LB@@LB@Position - mRNA 88 -- 104@LB@Position - sRNA 15 -- 30@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 15 -- 21@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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