| 3 |
5.299e-07 |
0.0005893 |
bsu18000 |
citB |
-22.45 |
0.000394 |
173 -- 202 |
21 -- 50 |
aconitate hydratase |
|
_____________172______________________________203\n_____________|________________________________|\n5'-UCU...AGAAU____________________AUU____A____GGCAA...CGU-3'\n______________GAUC_UGAUCUGA_AGGGGG___UUGG_GAAU\n______________||||_||||||||_||:|||___||||_|||:\n______________CUAG_ACUAGACU_UCUCCC___AACC_CUUG\n3'-UUU...AAAGC____A________A______C______C____UCUCU...AUA-5'\n_____________|________________________________|\n_____________51_______________________________20\n |
178 -- 184 |
38 -- 44 |
-32.0259 kcal/mol |
6.8093 kcal/mol |
2.76846 kcal/mol |
172 203@LB@ | |@LB@5'-UCU...AGAAU AUU A GGCAA...CGU-3'@LB@ GAUC UGAUCUGA AGGGGG UUGG GAAU@LB@ |||| |||||||| ||:||| |||| |||:@LB@ CUAG ACUAGACU UCUCCC AACC CUUG@LB@3'-UUU...AAAGC A A C C UCUCU...AUA-5'@LB@ | |@LB@ 51 20@LB@@LB@@LB@Energy -22.45 kcal/mol@LB@Hybridization Energy -32.0259 kcal/mol@LB@Unfolding Energy - mRNA 6.8093 kcal/mol@LB@Unfolding Energy - sRNA 2.76846 kcal/mol@LB@@LB@Position - mRNA 173 -- 202@LB@Position - sRNA 21 -- 50@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 78 |
0.005707 |
0.2441 |
bsu18040 |
yneP |
-11.92 |
0.108570 |
96 -- 119 |
24 -- 45 |
acyl-CoA thioesterase |
|
_____________95_______________________120\n_____________|________________________|\n5'-UUG...CUUUC_________UAU__UAAAA_____CUAAA...AAA-3'\n______________UGAUUUGAU___GG_____UGGGG\n______________||||:||||___||_____|||||\n______________ACUAGACUA___CC_____ACCCC\n3'-UUU...CUAGA_________UCU__CCA_______UUGUC...AUA-5'\n_____________|________________________|\n_____________46_______________________23\n |
98 -- 104 |
37 -- 43 |
-18.893 kcal/mol |
5.99443 kcal/mol |
0.977634 kcal/mol |
95 120@LB@ | |@LB@5'-UUG...CUUUC UAU UAAAA CUAAA...AAA-3'@LB@ UGAUUUGAU GG UGGGG@LB@ ||||:|||| || |||||@LB@ ACUAGACUA CC ACCCC@LB@3'-UUU...CUAGA UCU CCA UUGUC...AUA-5'@LB@ | |@LB@ 46 23@LB@@LB@@LB@Energy -11.92 kcal/mol@LB@Hybridization Energy -18.893 kcal/mol@LB@Unfolding Energy - mRNA 5.99443 kcal/mol@LB@Unfolding Energy - sRNA 0.977634 kcal/mol@LB@@LB@Position - mRNA 96 -- 119@LB@Position - sRNA 24 -- 45@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 110 |
0.009311 |
0.2778 |
bsu18230 |
yngG |
-6.09 |
0.597733 |
269 -- 285 |
32 -- 53 |
hydroxymethylglutaryl-CoA lyase YngG |
|
_____________268____________________286\n_____________|______________________|\n5'-AAA...CCCGU_______GCAAC__________AUAAA...GGA-3'\n______________UUGGAUC__________AGAGG\n______________|:|||||__________|||||\n______________AGCCUAG__________UCUCC\n3'-UUU...AAAAA_______AACUAGACUA_____CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
269 -- 275 |
47 -- 53 |
-12.5955 kcal/mol |
5.52332 kcal/mol |
0.981755 kcal/mol |
268 286@LB@ | |@LB@5'-AAA...CCCGU GCAAC AUAAA...GGA-3'@LB@ UUGGAUC AGAGG@LB@ |:||||| |||||@LB@ AGCCUAG UCUCC@LB@3'-UUU...AAAAA AACUAGACUA CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -6.09 kcal/mol@LB@Hybridization Energy -12.5955 kcal/mol@LB@Unfolding Energy - mRNA 5.52332 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 269 -- 285@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 269 -- 275@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 40 |
0.001724 |
0.1438 |
bsu18440 |
gltD |
-13.77 |
0.051762 |
193 -- 208 |
22 -- 36 |
glutamate synthase [NADPH] small chain |
|
_____________192______________209\n_____________|________________|\n5'-AGA...AGGGG_____AACA_______ACCAA...CUU-3'\n______________AGAGG____UGGGGAA\n______________|||||____|||||||\n______________UCUCC____ACCCCUU\n3'-UUU...GACUA_____CCA________GUCUC...AUA-5'\n_____________|________________|\n_____________37_______________21\n |
202 -- 208 |
22 -- 28 |
-17.8722 kcal/mol |
3.06294 kcal/mol |
1.03819 kcal/mol |
192 209@LB@ | |@LB@5'-AGA...AGGGG AACA ACCAA...CUU-3'@LB@ AGAGG UGGGGAA@LB@ ||||| |||||||@LB@ UCUCC ACCCCUU@LB@3'-UUU...GACUA CCA GUCUC...AUA-5'@LB@ | |@LB@ 37 21@LB@@LB@@LB@Energy -13.77 kcal/mol@LB@Hybridization Energy -17.8722 kcal/mol@LB@Unfolding Energy - mRNA 3.06294 kcal/mol@LB@Unfolding Energy - sRNA 1.03819 kcal/mol@LB@@LB@Position - mRNA 193 -- 208@LB@Position - sRNA 22 -- 36@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 22 -- 28@LB@ |
| 122 |
0.01077 |
0.2883 |
bsu18910 |
rapK |
-11.72 |
0.116857 |
189 -- 197 |
28 -- 36 |
response regulator aspartate phosphatase K |
|
_____________188_______198\n_____________|_________|\n5'-UAU...CAGAA_________UAAAU...CAG-3'\n______________AGAGGGGUU\n______________|||||||||\n______________UCUCCCCAA\n3'-UUU...GACUA_________CCCCU...AUA-5'\n_____________|_________|\n_____________37________27\n |
191 -- 197 |
28 -- 34 |
-15.633 kcal/mol |
3.77416 kcal/mol |
0.134381 kcal/mol |
188 198@LB@ | |@LB@5'-UAU...CAGAA UAAAU...CAG-3'@LB@ AGAGGGGUU@LB@ |||||||||@LB@ UCUCCCCAA@LB@3'-UUU...GACUA CCCCU...AUA-5'@LB@ | |@LB@ 37 27@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -15.633 kcal/mol@LB@Unfolding Energy - mRNA 3.77416 kcal/mol@LB@Unfolding Energy - sRNA 0.134381 kcal/mol@LB@@LB@Position - mRNA 189 -- 197@LB@Position - sRNA 28 -- 36@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 15 |
0.0002364 |
0.05257 |
bsu19290 |
yozO |
-13.57 |
0.056229 |
184 -- 195 |
28 -- 39 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________183__________196\n_____________|____________|\n5'-AAA...AUGCA__A_________CUUCA...UGG-3'\n______________GA_AGAGGGGUU\n______________||_|||||||||\n______________CU_UCUCCCCAA\n3'-UUU...CUAGA__A_________CCCCU...AUA-5'\n_____________|____________|\n_____________40___________27\n |
189 -- 195 |
28 -- 34 |
-16.9963 kcal/mol |
2.64187 kcal/mol |
0.77983 kcal/mol |
183 196@LB@ | |@LB@5'-AAA...AUGCA A CUUCA...UGG-3'@LB@ GA AGAGGGGUU@LB@ || |||||||||@LB@ CU UCUCCCCAA@LB@3'-UUU...CUAGA A CCCCU...AUA-5'@LB@ | |@LB@ 40 27@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -16.9963 kcal/mol@LB@Unfolding Energy - mRNA 2.64187 kcal/mol@LB@Unfolding Energy - sRNA 0.77983 kcal/mol@LB@@LB@Position - mRNA 184 -- 195@LB@Position - sRNA 28 -- 39@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 98 |
0.008247 |
0.2778 |
bsu19360 |
odhB |
-11.30 |
0.136272 |
146 -- 191 |
23 -- 51 |
dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex |
|
_____________145____________________________________________192\n_____________|______________________________________________|\n5'-AAG...GAACA___ACGUA___U_________CUU_ACUCGCAAAAACUAAG_____AAUGA...AAU-3'\n______________GGA_____UUG_AUCUGAUAG___G________________GGGGA\n______________|||_____|||_|||||||||___|________________|||||\n______________CCU_____AAC_UAGACUAUC___C________________CCCCU\n3'-UUU...AAAAG___AG________________UCC_CAA__________________UGUCU...AUA-5'\n_____________|______________________________________________|\n_____________52_____________________________________________22\n |
160 -- 166 |
35 -- 41 |
-19.511 kcal/mol |
7.22607 kcal/mol |
0.980049 kcal/mol |
145 192@LB@ | |@LB@5'-AAG...GAACA ACGUA U CUU ACUCGCAAAAACUAAG AAUGA...AAU-3'@LB@ GGA UUG AUCUGAUAG G GGGGA@LB@ ||| ||| ||||||||| | |||||@LB@ CCU AAC UAGACUAUC C CCCCU@LB@3'-UUU...AAAAG AG UCC CAA UGUCU...AUA-5'@LB@ | |@LB@ 52 22@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -19.511 kcal/mol@LB@Unfolding Energy - mRNA 7.22607 kcal/mol@LB@Unfolding Energy - sRNA 0.980049 kcal/mol@LB@@LB@Position - mRNA 146 -- 191@LB@Position - sRNA 23 -- 51@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 118 |
0.01022 |
0.2883 |
bsu19370 |
sucA |
-9.59 |
0.243554 |
183 -- 190 |
23 -- 30 |
2-oxoglutarate dehydrogenase subunit E1 |
|
_____________182______191\n_____________|________|\n5'-UUU...UUUAA________UAAUA...AUC-3'\n______________GUUGGGGG\n______________|||||||:\n______________CAACCCCU\n3'-UUU...CUCCC________UGUCU...AUA-5'\n_____________|________|\n_____________31_______22\n |
184 -- 190 |
23 -- 29 |
-13.0923 kcal/mol |
3.48895 kcal/mol |
0.018115 kcal/mol |
182 191@LB@ | |@LB@5'-UUU...UUUAA UAAUA...AUC-3'@LB@ GUUGGGGG@LB@ |||||||:@LB@ CAACCCCU@LB@3'-UUU...CUCCC UGUCU...AUA-5'@LB@ | |@LB@ 31 22@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -13.0923 kcal/mol@LB@Unfolding Energy - mRNA 3.48895 kcal/mol@LB@Unfolding Energy - sRNA 0.018115 kcal/mol@LB@@LB@Position - mRNA 183 -- 190@LB@Position - sRNA 23 -- 30@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 23 -- 29@LB@ |
| 99 |
0.00842 |
0.2778 |
bsu19470 |
yojF |
-13.80 |
0.051174 |
184 -- 193 |
30 -- 39 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________183________194\n_____________|__________|\n5'-GAA...AAGAA__A_______AGCAA...CAG-3'\n______________GA_AGAGGGG\n______________||_|||||||\n______________CU_UCUCCCC\n3'-UUU...CUAGA__A_______AACCC...AUA-5'\n_____________|__________|\n_____________40_________29\n |
187 -- 193 |
30 -- 36 |
-15.454 kcal/mol |
0.87683 kcal/mol |
0.779184 kcal/mol |
183 194@LB@ | |@LB@5'-GAA...AAGAA A AGCAA...CAG-3'@LB@ GA AGAGGGG@LB@ || |||||||@LB@ CU UCUCCCC@LB@3'-UUU...CUAGA A AACCC...AUA-5'@LB@ | |@LB@ 40 29@LB@@LB@@LB@Energy -13.80 kcal/mol@LB@Hybridization Energy -15.454 kcal/mol@LB@Unfolding Energy - mRNA 0.87683 kcal/mol@LB@Unfolding Energy - sRNA 0.779184 kcal/mol@LB@@LB@Position - mRNA 184 -- 193@LB@Position - sRNA 30 -- 39@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 38 |
0.001645 |
0.1432 |
bsu19540 |
yodB |
-7.66 |
0.420262 |
259 -- 271 |
37 -- 51 |
ArsR family transcriptional regulator |
|
_____________258_____________272\n_____________|_______________|\n5'-GAG...ACGAU___AC__________CAUCC...AAC-3'\n______________GGA____GGUCUGAU\n______________|||____|:||||||\n______________CCU____CUAGACUA\n3'-UUU...AAAAG___AGAA________UCUCC...AUA-5'\n_____________|_______________|\n_____________52______________36\n |
265 -- 271 |
37 -- 43 |
-11.6813 kcal/mol |
3.05786 kcal/mol |
0.959935 kcal/mol |
258 272@LB@ | |@LB@5'-GAG...ACGAU AC CAUCC...AAC-3'@LB@ GGA GGUCUGAU@LB@ ||| |:||||||@LB@ CCU CUAGACUA@LB@3'-UUU...AAAAG AGAA UCUCC...AUA-5'@LB@ | |@LB@ 52 36@LB@@LB@@LB@Energy -7.66 kcal/mol@LB@Hybridization Energy -11.6813 kcal/mol@LB@Unfolding Energy - mRNA 3.05786 kcal/mol@LB@Unfolding Energy - sRNA 0.959935 kcal/mol@LB@@LB@Position - mRNA 259 -- 271@LB@Position - sRNA 37 -- 51@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 37 -- 43@LB@ |
