| 133 |
0.01175 |
0.2883 |
bsu11610 |
ppnK |
-12.45 |
0.088788 |
273 -- 294 |
32 -- 53 |
inorganic polyphosphate/ATP-NAD kinase 1 |
|
_____________272____________________295\n_____________|______________________|\n5'-CCA...AUUUA____________A___AACU__AUGAAA-3'\n______________UUGGAUUUUGAU_UGG____GG\n______________|:||||:|||||_||:____||\n______________AGCCUAGAACUA_ACU____CC\n3'-UUU...AAAAA____________G___AUCU__CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
278 -- 284 |
42 -- 48 |
-17.5759 kcal/mol |
4.14878 kcal/mol |
0.981755 kcal/mol |
272 295@LB@ | |@LB@5'-CCA...AUUUA A AACU AUGAAA-3'@LB@ UUGGAUUUUGAU UGG GG@LB@ |:||||:||||| ||: ||@LB@ AGCCUAGAACUA ACU CC@LB@3'-UUU...AAAAA G AUCU CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -12.45 kcal/mol@LB@Hybridization Energy -17.5759 kcal/mol@LB@Unfolding Energy - mRNA 4.14878 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 273 -- 294@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 49 |
0.002227 |
0.1516 |
bsu11839 |
yjzE |
-19.04 |
0.003744 |
170 -- 194 |
25 -- 51 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 11976315 |
|
_____________169_________________________195\n_____________|___________________________|\n5'-UCC...UUAAG___AAA___GACAGC____________UAUUG...GCU-3'\n______________GGA____GA______AGAGGGGU_GGG\n______________|||____||______||||||||_|||\n______________CCU____CU______UCUCCCCA_CCC\n3'-UUU...AAAAG___AGAA__AGACUA________A___CUUGU...AUA-5'\n_____________|___________________________|\n_____________52__________________________24\n |
185 -- 191 |
29 -- 35 |
-23.53 kcal/mol |
3.51134 kcal/mol |
0.976067 kcal/mol |
169 195@LB@ | |@LB@5'-UCC...UUAAG AAA GACAGC UAUUG...GCU-3'@LB@ GGA GA AGAGGGGU GGG@LB@ ||| || |||||||| |||@LB@ CCU CU UCUCCCCA CCC@LB@3'-UUU...AAAAG AGAA AGACUA A CUUGU...AUA-5'@LB@ | |@LB@ 52 24@LB@@LB@@LB@Energy -19.04 kcal/mol@LB@Hybridization Energy -23.53 kcal/mol@LB@Unfolding Energy - mRNA 3.51134 kcal/mol@LB@Unfolding Energy - sRNA 0.976067 kcal/mol@LB@@LB@Position - mRNA 170 -- 194@LB@Position - sRNA 25 -- 51@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 6 |
3.166e-05 |
0.01761 |
bsu12160 |
yjgC |
-18.77 |
0.004394 |
190 -- 206 |
10 -- 27 |
oxidoreductase |
bsu27220 bsu03310 |
_____________189________________207\n_____________|__________________|\n5'-UAA...AGAAA__________________GGCAA...UGC-3'\n______________GGGGAAUGGAGA_UGGCU\n______________||||||::||||_|:|||\n______________CCCCUUGUCUCU_AUCGA\n3'-UUU...CCCAA____________C_____AGAGA...AUA-5'\n_____________|__________________|\n_____________28_________________9\n |
192 -- 198 |
19 -- 25 |
-27.0798 kcal/mol |
2.28534 kcal/mol |
6.0284 kcal/mol |
189 207@LB@ | |@LB@5'-UAA...AGAAA GGCAA...UGC-3'@LB@ GGGGAAUGGAGA UGGCU@LB@ ||||||::|||| |:|||@LB@ CCCCUUGUCUCU AUCGA@LB@3'-UUU...CCCAA C AGAGA...AUA-5'@LB@ | |@LB@ 28 9@LB@@LB@@LB@Energy -18.77 kcal/mol@LB@Hybridization Energy -27.0798 kcal/mol@LB@Unfolding Energy - mRNA 2.28534 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 190 -- 206@LB@Position - sRNA 10 -- 27@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 108 |
0.009234 |
0.2778 |
bsu12360 |
exuT |
-9.24 |
0.271038 |
118 -- 128 |
32 -- 43 |
hexuronate transporter |
|
_____________117__________129\n_____________|____________|\n5'-AAA...UUCCU____________AUUUA...UUA-3'\n______________GUC_GAUGGAGG\n______________:||_|||:||||\n______________UAG_CUAUCUCC\n3'-UUU...AGAAC___A________CCAAC...AUA-5'\n_____________|____________|\n_____________44___________31\n |
122 -- 128 |
32 -- 38 |
-13.9988 kcal/mol |
3.80022 kcal/mol |
0.959872 kcal/mol |
117 129@LB@ | |@LB@5'-AAA...UUCCU AUUUA...UUA-3'@LB@ GUC GAUGGAGG@LB@ :|| |||:||||@LB@ UAG CUAUCUCC@LB@3'-UUU...AGAAC A CCAAC...AUA-5'@LB@ | |@LB@ 44 31@LB@@LB@@LB@Energy -9.24 kcal/mol@LB@Hybridization Energy -13.9988 kcal/mol@LB@Unfolding Energy - mRNA 3.80022 kcal/mol@LB@Unfolding Energy - sRNA 0.959872 kcal/mol@LB@@LB@Position - mRNA 118 -- 128@LB@Position - sRNA 32 -- 43@LB@Position Seed - mRNA 122 -- 128@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 82 |
0.006245 |
0.254 |
bsu12400 |
yjnA |
-14.90 |
0.031572 |
235 -- 273 |
19 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________234_____________________________________274\n_____________|_______________________________________|\n5'-UUA...GUUUG_____GC___A___G___U__C___A__G__U_______CCUUA...UGC-3'\n______________UCGGA__CUU_GUC_GAU_GA_GGG_GU_GG_GGAGCGG\n______________|||||__|||_:||_|||_||_|||_||_||_|||:|:|\n______________AGCCU__GAA_UAG_CUA_CU_CCC_CA_CC_CCUUGUC\n3'-UUU...AAAAA_____A____C___A___U_________A__________UCUCA...AUA-5'\n_____________|_______________________________________|\n_____________54______________________________________18\n |
267 -- 273 |
19 -- 25 |
-33.8463 kcal/mol |
12.0212 kcal/mol |
6.92635 kcal/mol |
234 274@LB@ | |@LB@5'-UUA...GUUUG GC A G U C A G U CCUUA...UGC-3'@LB@ UCGGA CUU GUC GAU GA GGG GU GG GGAGCGG@LB@ ||||| ||| :|| ||| || ||| || || |||:|:|@LB@ AGCCU GAA UAG CUA CU CCC CA CC CCUUGUC@LB@3'-UUU...AAAAA A C A U A UCUCA...AUA-5'@LB@ | |@LB@ 54 18@LB@@LB@@LB@Energy -14.90 kcal/mol@LB@Hybridization Energy -33.8463 kcal/mol@LB@Unfolding Energy - mRNA 12.0212 kcal/mol@LB@Unfolding Energy - sRNA 6.92635 kcal/mol@LB@@LB@Position - mRNA 235 -- 273@LB@Position - sRNA 19 -- 53@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 192 |
0.01921 |
0.3269 |
bsu12649 |
ykzM |
-11.69 |
0.118448 |
82 -- 103 |
11 -- 32 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu26089 |
_____________81______________________104\n_____________|_______________________|\n5'-AAG...GACAA____A____________C_A___GGCCA...UCG-3'\n______________GGGU__GGGGACAGGGG_G_GGC\n______________||||__|||:||||:|:_|_:||\n______________CCCA__CCCUUGUCUCU_C_UCG\n3'-UUU...AUCUC____AC_____________A___AAGAG...AUA-5'\n_____________|_______________________|\n_____________33______________________10\n |
88 -- 94 |
19 -- 25 |
-26.088 kcal/mol |
7.89329 kcal/mol |
6.50677 kcal/mol |
81 104@LB@ | |@LB@5'-AAG...GACAA A C A GGCCA...UCG-3'@LB@ GGGU GGGGACAGGGG G GGC@LB@ |||| |||:||||:|: | :||@LB@ CCCA CCCUUGUCUCU C UCG@LB@3'-UUU...AUCUC AC A AAGAG...AUA-5'@LB@ | |@LB@ 33 10@LB@@LB@@LB@Energy -11.69 kcal/mol@LB@Hybridization Energy -26.088 kcal/mol@LB@Unfolding Energy - mRNA 7.89329 kcal/mol@LB@Unfolding Energy - sRNA 6.50677 kcal/mol@LB@@LB@Position - mRNA 82 -- 103@LB@Position - sRNA 11 -- 32@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 107 |
0.009163 |
0.2778 |
bsu12780 |
xepA |
-15.86 |
0.020153 |
79 -- 99 |
29 -- 48 |
phage-like element PBSX protein XepA |
|
_____________78____________________100\n_____________|_____________________|\n5'-UUG...UCAAU__AG_______C______U__GAACA...UUU-3'\n______________UC__GAUCUGG_AGAGGG_GU\n______________||__||||||:_||||||_||\n______________AG__CUAGACU_UCUCCC_CA\n3'-UUU...AGCCU__AA_______A_________ACCCC...AUA-5'\n_____________|_____________________|\n_____________49____________________28\n |
83 -- 89 |
38 -- 44 |
-22.9588 kcal/mol |
6.12286 kcal/mol |
0.974361 kcal/mol |
78 100@LB@ | |@LB@5'-UUG...UCAAU AG C U GAACA...UUU-3'@LB@ UC GAUCUGG AGAGGG GU@LB@ || ||||||: |||||| ||@LB@ AG CUAGACU UCUCCC CA@LB@3'-UUU...AGCCU AA A ACCCC...AUA-5'@LB@ | |@LB@ 49 28@LB@@LB@@LB@Energy -15.86 kcal/mol@LB@Hybridization Energy -22.9588 kcal/mol@LB@Unfolding Energy - mRNA 6.12286 kcal/mol@LB@Unfolding Energy - sRNA 0.974361 kcal/mol@LB@@LB@Position - mRNA 79 -- 99@LB@Position - sRNA 29 -- 48@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 147 |
0.013 |
0.2949 |
bsu12910 |
proG |
-10.46 |
0.183122 |
211 -- 241 |
23 -- 53 |
pyrroline-5-carboxylate reductase 3 |
|
_____________210________________________________242\n_____________|__________________________________|\n5'-UUC...ACAGA_____________G____U___CAGCA___C___UAUGA...UUG-3'\n______________UUGGAUU__GAUU_GAUA__GG_____UGG_GGA\n______________|:||||:__|||:_||||__||_____|||_|||\n______________AGCCUAG__CUAG_CUAU__CC_____ACC_CCU\n3'-UUU...AAAAA_______AA____A____CU__CCA_________UGUCU...AUA-5'\n_____________|__________________________________|\n_____________54_________________________________22\n |
211 -- 217 |
47 -- 53 |
-18.7228 kcal/mol |
7.27559 kcal/mol |
0.987321 kcal/mol |
210 242@LB@ | |@LB@5'-UUC...ACAGA G U CAGCA C UAUGA...UUG-3'@LB@ UUGGAUU GAUU GAUA GG UGG GGA@LB@ |:||||: |||: |||| || ||| |||@LB@ AGCCUAG CUAG CUAU CC ACC CCU@LB@3'-UUU...AAAAA AA A CU CCA UGUCU...AUA-5'@LB@ | |@LB@ 54 22@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -18.7228 kcal/mol@LB@Unfolding Energy - mRNA 7.27559 kcal/mol@LB@Unfolding Energy - sRNA 0.987321 kcal/mol@LB@@LB@Position - mRNA 211 -- 241@LB@Position - sRNA 23 -- 53@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 170 |
0.0164 |
0.3152 |
bsu13090 |
ykkC |
-8.27 |
0.358617 |
162 -- 212 |
23 -- 55 |
multidrug resistance protein |
|
_____________161__________________________________________________213\n_____________|____________________________________________________|\n5'-UCA...UUUGU_______GAA____A__A_______AA__AAUGACUCAAAAU_AAA______UUGGU...CCA-3'\n______________UUUCGGA___UCUU_AU__GAUAGA__GG_____________G___UGGGGA\n______________|||||||___||||_||__||||||__||_____________|___||||||\n______________AAAGCCU___AGAA_UA__CUAUCU__CC_____________C___ACCCCU\n3'-UUU...UCAAA______________C__GA______C_________________A________UGUCU...AUA-5'\n_____________|____________________________________________________|\n_____________56___________________________________________________22\n |
162 -- 168 |
49 -- 55 |
-21.902 kcal/mol |
11.1231 kcal/mol |
2.51158 kcal/mol |
161 213@LB@ | |@LB@5'-UCA...UUUGU GAA A A AA AAUGACUCAAAAU AAA UUGGU...CCA-3'@LB@ UUUCGGA UCUU AU GAUAGA GG G UGGGGA@LB@ ||||||| |||| || |||||| || | ||||||@LB@ AAAGCCU AGAA UA CUAUCU CC C ACCCCU@LB@3'-UUU...UCAAA C GA C A UGUCU...AUA-5'@LB@ | |@LB@ 56 22@LB@@LB@@LB@Energy -8.27 kcal/mol@LB@Hybridization Energy -21.902 kcal/mol@LB@Unfolding Energy - mRNA 11.1231 kcal/mol@LB@Unfolding Energy - sRNA 2.51158 kcal/mol@LB@@LB@Position - mRNA 162 -- 212@LB@Position - sRNA 23 -- 55@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 112 |
0.009326 |
0.2778 |
bsu13200 |
rsbRB |
-15.32 |
0.026068 |
265 -- 282 |
32 -- 51 |
RsbT co-antagonist protein RsbRB |
bsu19450 |
_____________264__________________283\n_____________|____________________|\n5'-CUU...GGAAU_________AAC________AAAGU...CGC-3'\n______________GGAU__UGA___GAUGGAGG\n______________||||__|||___|||:||||\n______________CCUA__ACU___CUAUCUCC\n3'-UUU...AAAAG____GA___AGA________CCAAC...AUA-5'\n_____________|____________________|\n_____________52___________________31\n |
276 -- 282 |
32 -- 38 |
-18.0013 kcal/mol |
1.71479 kcal/mol |
0.97021 kcal/mol |
264 283@LB@ | |@LB@5'-CUU...GGAAU AAC AAAGU...CGC-3'@LB@ GGAU UGA GAUGGAGG@LB@ |||| ||| |||:||||@LB@ CCUA ACU CUAUCUCC@LB@3'-UUU...AAAAG GA AGA CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -15.32 kcal/mol@LB@Hybridization Energy -18.0013 kcal/mol@LB@Unfolding Energy - mRNA 1.71479 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 265 -- 282@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 32 -- 38@LB@ |
