| 143 |
0.01244 |
0.2883 |
bsu13980 |
pbpH |
-18.09 |
0.006437 |
121 -- 135 |
29 -- 45 |
penicillin-binding enzyme for formation of rod-shaped peptidoglycan cell wall |
|
_____________120_______________136\n_____________|_________________|\n5'-UUU...UUGAA_________________AACCA...ACG-3'\n______________UGAUUUGA__GAGGGGU\n______________||||:|||__|||||||\n______________ACUAGACU__CUCCCCA\n3'-UUU...CUAGA________AU_______ACCCC...AUA-5'\n_____________|_________________|\n_____________46________________28\n |
129 -- 135 |
29 -- 35 |
-21.9726 kcal/mol |
2.91712 kcal/mol |
0.969832 kcal/mol |
120 136@LB@ | |@LB@5'-UUU...UUGAA AACCA...ACG-3'@LB@ UGAUUUGA GAGGGGU@LB@ ||||:||| |||||||@LB@ ACUAGACU CUCCCCA@LB@3'-UUU...CUAGA AU ACCCC...AUA-5'@LB@ | |@LB@ 46 28@LB@@LB@@LB@Energy -18.09 kcal/mol@LB@Hybridization Energy -21.9726 kcal/mol@LB@Unfolding Energy - mRNA 2.91712 kcal/mol@LB@Unfolding Energy - sRNA 0.969832 kcal/mol@LB@@LB@Position - mRNA 121 -- 135@LB@Position - sRNA 29 -- 45@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 168 |
0.01631 |
0.3152 |
bsu14170 |
ykuP |
-12.43 |
0.089417 |
80 -- 100 |
11 -- 27 |
flavodoxin-2 |
|
_____________79____________________101\n_____________|_____________________|\n5'-CAC...AUUAA___ACAU______________CAAUC...UAG-3'\n______________GGG____GGAUGGAGAGUGGC\n______________|||____|:|::||||||:||\n______________CCC____CUUGUCUCUCAUCG\n3'-UUU...CCCAA_____________________AAGAG...AUA-5'\n_____________|_____________________|\n_____________28____________________10\n |
94 -- 100 |
11 -- 17 |
-24.7716 kcal/mol |
6.31596 kcal/mol |
6.0284 kcal/mol |
79 101@LB@ | |@LB@5'-CAC...AUUAA ACAU CAAUC...UAG-3'@LB@ GGG GGAUGGAGAGUGGC@LB@ ||| |:|::||||||:||@LB@ CCC CUUGUCUCUCAUCG@LB@3'-UUU...CCCAA AAGAG...AUA-5'@LB@ | |@LB@ 28 10@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -24.7716 kcal/mol@LB@Unfolding Energy - mRNA 6.31596 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 80 -- 100@LB@Position - sRNA 11 -- 27@LB@Position Seed - mRNA 94 -- 100@LB@Position Seed - sRNA 11 -- 17@LB@ |
| 76 |
0.005654 |
0.2441 |
bsu14240 |
rok |
-14.05 |
0.045854 |
178 -- 222 |
9 -- 42 |
repressor rok |
|
_____________177______________________________________________223\n_____________|________________________________________________|\n5'-UUA...UUAAU______________ACAU__A_____GUUUAAUGAA____________UGCGC...UAU-3'\n______________UUUGAUAG_GGGGU____UG_GGAAU__________AGAGA__AGCUU\n______________|:||||||_|||||____||_||||:__________|||||__|||||\n______________AGACUAUC_CCCCA____AC_CCUUG__________UCUCU__UCGAA\n3'-UUU...GAACU________U___________C____________________CA_____GAGAGAUA-5'\n_____________|________________________________________________|\n_____________43_______________________________________________8\n |
179 -- 185 |
35 -- 41 |
-31.6296 kcal/mol |
7.67652 kcal/mol |
9.89852 kcal/mol |
177 223@LB@ | |@LB@5'-UUA...UUAAU ACAU A GUUUAAUGAA UGCGC...UAU-3'@LB@ UUUGAUAG GGGGU UG GGAAU AGAGA AGCUU@LB@ |:|||||| ||||| || ||||: ||||| |||||@LB@ AGACUAUC CCCCA AC CCUUG UCUCU UCGAA@LB@3'-UUU...GAACU U C CA GAGAGAUA-5'@LB@ | |@LB@ 43 8@LB@@LB@@LB@Energy -14.05 kcal/mol@LB@Hybridization Energy -31.6296 kcal/mol@LB@Unfolding Energy - mRNA 7.67652 kcal/mol@LB@Unfolding Energy - sRNA 9.89852 kcal/mol@LB@@LB@Position - mRNA 178 -- 222@LB@Position - sRNA 9 -- 42@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 84 |
0.006671 |
0.2588 |
bsu14270 |
moeB |
-18.74 |
0.004465 |
187 -- 207 |
32 -- 51 |
molybdopterin-synthase adenylyltransferase |
|
_____________186___________________208\n_____________|_____________________|\n5'-UUG...GAUAA___GAACA_____________AACGU...GAG-3'\n______________GGA_____GGUUUGAUGGAGG\n______________|||_____|:|:||||:||||\n______________CCU_____CUAGACUAUCUCC\n3'-UUU...AAAAG___AGAA______________CCAAC...AUA-5'\n_____________|_____________________|\n_____________52____________________31\n |
201 -- 207 |
32 -- 38 |
-23.059 kcal/mol |
3.35069 kcal/mol |
0.97021 kcal/mol |
186 208@LB@ | |@LB@5'-UUG...GAUAA GAACA AACGU...GAG-3'@LB@ GGA GGUUUGAUGGAGG@LB@ ||| |:|:||||:||||@LB@ CCU CUAGACUAUCUCC@LB@3'-UUU...AAAAG AGAA CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -18.74 kcal/mol@LB@Hybridization Energy -23.059 kcal/mol@LB@Unfolding Energy - mRNA 3.35069 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 187 -- 207@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 33 |
0.001178 |
0.119 |
bsu14840 |
ylaN |
-15.06 |
0.029338 |
181 -- 208 |
8 -- 28 |
depletion of this protein in Bacillus subtilis results in defects in cell morphology; crystal structure of Staphylococcus protein shows homodimer; ligand binding protein |
|
_____________180__________________________209\n_____________|____________________________|\n5'-GAU...UCGCC______________AUACUUU_______AGAGA...AUA-3'\n______________UGGGGAACGGAGGG_______UGGCUUC\n______________||||||||:|||:|_______|:|||||\n______________ACCCCUUGUCUCUC_______AUCGAAG\n3'-UUU...CCCCA____________________________AGAGAUA-5'\n_____________|____________________________|\n_____________29___________________________7\n |
202 -- 208 |
8 -- 14 |
-32.8109 kcal/mol |
11.1547 kcal/mol |
6.59627 kcal/mol |
180 209@LB@ | |@LB@5'-GAU...UCGCC AUACUUU AGAGA...AUA-3'@LB@ UGGGGAACGGAGGG UGGCUUC@LB@ ||||||||:|||:| |:|||||@LB@ ACCCCUUGUCUCUC AUCGAAG@LB@3'-UUU...CCCCA AGAGAUA-5'@LB@ | |@LB@ 29 7@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -32.8109 kcal/mol@LB@Unfolding Energy - mRNA 11.1547 kcal/mol@LB@Unfolding Energy - sRNA 6.59627 kcal/mol@LB@@LB@Position - mRNA 181 -- 208@LB@Position - sRNA 8 -- 28@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 8 -- 14@LB@ |
| 194 |
0.01929 |
0.3269 |
bsu14860 |
pycA |
-10.87 |
0.159030 |
149 -- 193 |
23 -- 51 |
pyruvate carboxylase |
|
_____________148___________________________________________194\n_____________|_____________________________________________|\n5'-GGG...GGGAA____AUUCCGCUA_______UUAUAUUCCAUUC____AAG_____GAAAA...UUG-3'\n______________GGAU_________UGAUAGA_____________GGGU___GGGGA\n______________||||_________|||||||_____________||||___|||||\n______________CCUA_________ACUAUCU_____________CCCA___CCCCU\n3'-UUU...AAAAG____GAACUAG_________C________________A_______UGUCU...AUA-5'\n_____________|_____________________________________________|\n_____________52____________________________________________22\n |
162 -- 168 |
34 -- 40 |
-22.9068 kcal/mol |
11.0566 kcal/mol |
0.980049 kcal/mol |
148 194@LB@ | |@LB@5'-GGG...GGGAA AUUCCGCUA UUAUAUUCCAUUC AAG GAAAA...UUG-3'@LB@ GGAU UGAUAGA GGGU GGGGA@LB@ |||| ||||||| |||| |||||@LB@ CCUA ACUAUCU CCCA CCCCU@LB@3'-UUU...AAAAG GAACUAG C A UGUCU...AUA-5'@LB@ | |@LB@ 52 22@LB@@LB@@LB@Energy -10.87 kcal/mol@LB@Hybridization Energy -22.9068 kcal/mol@LB@Unfolding Energy - mRNA 11.0566 kcal/mol@LB@Unfolding Energy - sRNA 0.980049 kcal/mol@LB@@LB@Position - mRNA 149 -- 193@LB@Position - sRNA 23 -- 51@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 74 |
0.005527 |
0.2441 |
bsu14880 |
ctaB |
-11.75 |
0.115909 |
1 -- 10 |
34 -- 43 |
protoheme IX farnesyltransferase 2 |
|
______________1_________11\n______________|_________|\n___________5'-__________AAUAA...UCG-3'\n______________GUCUGAUAGA\n______________:|||||||||\n______________UAGACUAUCU\n3'-UUU...AGAAC__________CCCCA...AUA-5'\n_____________|__________|\n_____________44_________33\n |
4 -- 10 |
34 -- 40 |
-13.2371 kcal/mol |
0.533082 kcal/mol |
0.957751 kcal/mol |
1 11@LB@ | |@LB@ 5'- AAUAA...UCG-3'@LB@ GUCUGAUAGA@LB@ :|||||||||@LB@ UAGACUAUCU@LB@3'-UUU...AGAAC CCCCA...AUA-5'@LB@ | |@LB@ 44 33@LB@@LB@@LB@Energy -11.75 kcal/mol@LB@Hybridization Energy -13.2371 kcal/mol@LB@Unfolding Energy - mRNA 0.533082 kcal/mol@LB@Unfolding Energy - sRNA 0.957751 kcal/mol@LB@@LB@Position - mRNA 1 -- 10@LB@Position - sRNA 34 -- 43@LB@Position Seed - mRNA 4 -- 10@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 1 |
3.729e-08 |
0.0001244 |
bsu14890 |
ctaC |
-17.27 |
0.009980 |
187 -- 199 |
23 -- 34 |
cytochrome c oxidase subunit 2 |
|
_____________186___________200\n_____________|_____________|\n5'-CGA...AGAAA__________U__AAUGG...GCG-3'\n______________AGGGGUUGGG_GA\n______________||||||||||_||\n______________UCCCCAACCC_CU\n3'-UUU...CUAUC_____________UGUCU...AUA-5'\n_____________|_____________|\n_____________35____________22\n |
190 -- 196 |
25 -- 31 |
-21.5157 kcal/mol |
4.12609 kcal/mol |
0.123213 kcal/mol |
186 200@LB@ | |@LB@5'-CGA...AGAAA U AAUGG...GCG-3'@LB@ AGGGGUUGGG GA@LB@ |||||||||| ||@LB@ UCCCCAACCC CU@LB@3'-UUU...CUAUC UGUCU...AUA-5'@LB@ | |@LB@ 35 22@LB@@LB@@LB@Energy -17.27 kcal/mol@LB@Hybridization Energy -21.5157 kcal/mol@LB@Unfolding Energy - mRNA 4.12609 kcal/mol@LB@Unfolding Energy - sRNA 0.123213 kcal/mol@LB@@LB@Position - mRNA 187 -- 199@LB@Position - sRNA 23 -- 34@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 25 -- 31@LB@ |
| 63 |
0.003897 |
0.2064 |
bsu14900 |
ctaD |
-14.39 |
0.039562 |
155 -- 192 |
22 -- 52 |
cytochrome c oxidase subunit 1 |
|
_____________154____________________________________193\n_____________|______________________________________|\n5'-GUG...AAAAG____AAGCAA__AG__A___AA___AGAUA________GGUCA...UGG-3'\n______________CGGA______GU__GG_AGA__GGG_____UUGGGGGA\n______________||||______:|__|:_|||__|||_____||||||:|\n______________GCCU______UA__CU_UCU__CCC_____AACCCCUU\n3'-UUU...AAAAA____AGAAC___GA__A___C_________________GUCUC...AUA-5'\n_____________|______________________________________|\n_____________53_____________________________________21\n |
186 -- 192 |
22 -- 28 |
-20.3858 kcal/mol |
4.11152 kcal/mol |
1.8808 kcal/mol |
154 193@LB@ | |@LB@5'-GUG...AAAAG AAGCAA AG A AA AGAUA GGUCA...UGG-3'@LB@ CGGA GU GG AGA GGG UUGGGGGA@LB@ |||| :| |: ||| ||| ||||||:|@LB@ GCCU UA CU UCU CCC AACCCCUU@LB@3'-UUU...AAAAA AGAAC GA A C GUCUC...AUA-5'@LB@ | |@LB@ 53 21@LB@@LB@@LB@Energy -14.39 kcal/mol@LB@Hybridization Energy -20.3858 kcal/mol@LB@Unfolding Energy - mRNA 4.11152 kcal/mol@LB@Unfolding Energy - sRNA 1.8808 kcal/mol@LB@@LB@Position - mRNA 155 -- 192@LB@Position - sRNA 22 -- 52@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 22 -- 28@LB@ |
| 44 |
0.001968 |
0.1492 |
bsu14940 |
ylbA |
-14.48 |
0.038144 |
221 -- 240 |
24 -- 52 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________220___________________________241\n_____________|_____________________________|\n5'-CAU...GAAAG_________________________AA__CUGAG...AAU-3'\n______________CGGA_CUUGA________AGGGGUU__GG\n______________||||_|||||________|||||||__||\n______________GCCU_GAACU________UCCCCAA__CC\n3'-UUU...AAAAA____A_____AGACUAUC_______CC__UUGUC...AUA-5'\n_____________|_____________________________|\n_____________53____________________________23\n |
230 -- 236 |
28 -- 34 |
-22.4266 kcal/mol |
6.96942 kcal/mol |
0.980851 kcal/mol |
220 241@LB@ | |@LB@5'-CAU...GAAAG AA CUGAG...AAU-3'@LB@ CGGA CUUGA AGGGGUU GG@LB@ |||| ||||| ||||||| ||@LB@ GCCU GAACU UCCCCAA CC@LB@3'-UUU...AAAAA A AGACUAUC CC UUGUC...AUA-5'@LB@ | |@LB@ 53 23@LB@@LB@@LB@Energy -14.48 kcal/mol@LB@Hybridization Energy -22.4266 kcal/mol@LB@Unfolding Energy - mRNA 6.96942 kcal/mol@LB@Unfolding Energy - sRNA 0.980851 kcal/mol@LB@@LB@Position - mRNA 221 -- 240@LB@Position - sRNA 24 -- 52@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 28 -- 34@LB@ |
