Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
118	0.008241	0.2451	b3394	hofN	-17.25	0.007000	1 -- 22	2 -- 26	DNA catabolic putative fimbrial assembly protein		______________1________________________23\n______________\|________________________\|\n___________5'-_____________CU__________GGAUG...CCG-3'\n______________UGGGGGCG_CAAA____GGCGGUGG\n______________\|\|\|\|\|:\|:_\|\|\|\|____:\|\|:\|\|\|\|\n______________ACCCCUGU_GUUU____UCGUCACC\n3'-UUU...GUUUU________A____CUUU________G-5'\n_____________\|_________________________\|\n_____________27________________________1\n	16 -- 22	2 -- 8	-29.7277 kcal/mol	9.95926 kcal/mol	2.5184 kcal/mol	1 23@LB@ \| \|@LB@ 5'- CU GGAUG...CCG-3'@LB@ UGGGGGCG CAAA GGCGGUGG@LB@ \|\|\|\|\|:\|: \|\|\|\| :\|\|:\|\|\|\|@LB@ ACCCCUGU GUUU UCGUCACC@LB@3'-UUU...GUUUU A CUUU G-5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -17.25 kcal/mol@LB@Hybridization Energy -29.7277 kcal/mol@LB@Unfolding Energy - mRNA 9.95926 kcal/mol@LB@Unfolding Energy - sRNA 2.5184 kcal/mol@LB@@LB@Position - mRNA 1 -- 22@LB@Position - sRNA 2 -- 26@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 2 -- 8@LB@
45	0.002187	0.1648	b3391	hofQ	-14.99	0.027914	2 -- 20	1 -- 20	DNA catabolic putative fimbrial transporter		_____________1____________________21\n_____________\|____________________\|\n__________5'-C__________U_________GAGGC...UAG-3'\n______________UAUCAGGGGA_GGUAG_GGC\n______________:\|\|\|\|::\|:\|_:\|:\|\|_\|\|\|\n______________GUAGUUUCUU_UCGUC_CCG\n3'-UUU...CCCCU__________U_____A___-5'\n_____________\|___________________\|\n_____________21__________________1\n	5 -- 11	11 -- 17	-22.4478 kcal/mol	5.99969 kcal/mol	1.45855 kcal/mol	1 21@LB@ \| \|@LB@ 5'-C U GAGGC...UAG-3'@LB@ UAUCAGGGGA GGUAG GGC@LB@ :\|\|\|\|::\|:\| :\|:\|\| \|\|\|@LB@ GUAGUUUCUU UCGUC CCG@LB@3'-UUU...CCCCU U A -5'@LB@ \| \|@LB@ 21 1@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -22.4478 kcal/mol@LB@Unfolding Energy - mRNA 5.99969 kcal/mol@LB@Unfolding Energy - sRNA 1.45855 kcal/mol@LB@@LB@Position - mRNA 2 -- 20@LB@Position - sRNA 1 -- 20@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 11 -- 17@LB@
67	0.003548	0.1858	b4412	hokC	-18.22	0.003564	16 -- 36	7 -- 32	toxic membrane protein small	b1562 b0018	_____________15_________________________37\n_____________\|__________________________\|\n5'-AUU...AUAAA__________________________GAUAU...ACA-3'\n______________ACAAAAUGGG____UUAAAGAGA_GC\n______________\|\|\|\|\|\|\|\|\|\|____\|:\|\|\|\|\|:\|_\|\|\n______________UGUUUUACCC____AGUUUCUUU_CG\n3'-UUU...CGAGG__________CUGU_________U__UCACCG-5'\n_____________\|__________________________\|\n_____________33_________________________6\n	28 -- 34	10 -- 16	-22.2189 kcal/mol	1.46836 kcal/mol	2.53437 kcal/mol	15 37@LB@ \| \|@LB@5'-AUU...AUAAA GAUAU...ACA-3'@LB@ ACAAAAUGGG UUAAAGAGA GC@LB@ \|\|\|\|\|\|\|\|\|\| \|:\|\|\|\|\|:\| \|\|@LB@ UGUUUUACCC AGUUUCUUU CG@LB@3'-UUU...CGAGG CUGU U UCACCG-5'@LB@ \| \|@LB@ 33 6@LB@@LB@@LB@Energy -18.22 kcal/mol@LB@Hybridization Energy -22.2189 kcal/mol@LB@Unfolding Energy - mRNA 1.46836 kcal/mol@LB@Unfolding Energy - sRNA 2.53437 kcal/mol@LB@@LB@Position - mRNA 16 -- 36@LB@Position - sRNA 7 -- 32@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 10 -- 16@LB@
195	0.01413	0.2529	b2527	hscB	-14.38	0.038945	62 -- 81	1 -- 21	HscA co-chaperone J domain-containing protein Hsc56; IscU-specific chaperone HscAB		_____________61____________________82\n_____________\|_____________________\|\n5'-GGU...CCCGA__________U__________UGCGG...CUG-3'\n______________ACGUCAAAGA_GAGU_GUGGU\n______________\|\|:\|\|\|\|\|\|\|_:\|\|:_\|\|\|\|:\n______________UGUAGUUUCU_UUCG_CACCG\n3'-UUU...ACCCC__________U____U_____-5'\n_____________\|____________________\|\n_____________22___________________1\n	65 -- 71	12 -- 18	-23.3701 kcal/mol	7.47956 kcal/mol	1.51103 kcal/mol	61 82@LB@ \| \|@LB@5'-GGU...CCCGA U UGCGG...CUG-3'@LB@ ACGUCAAAGA GAGU GUGGU@LB@ \|\|:\|\|\|\|\|\|\| :\|\|: \|\|\|\|:@LB@ UGUAGUUUCU UUCG CACCG@LB@3'-UUU...ACCCC U U -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -14.38 kcal/mol@LB@Hybridization Energy -23.3701 kcal/mol@LB@Unfolding Energy - mRNA 7.47956 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 62 -- 81@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 12 -- 18@LB@
9	0.0002541	0.09908	b4000	hupA	-15.77	0.017828	82 -- 110	1 -- 35	HU DNA-binding transcriptional regulator alpha subunit		_____________81__________________________________111\n_____________\|___________________________________\|\n5'-UUU...UCUGG______UUUG_________________AA______UAUCG...CUG-3'\n______________UCUACA____GGGGGCG______AAAA__AGUGGC\n______________\|\|:\|\|\|____\|\|\|\|:\|:______\|\|\|\|__\|\|\|\|\|\|\n______________AGGUGU____CCCCUGU______UUUU__UCACCG\n3'-UUU...ACCCG______UUUA_______AGUUUC____CG______-5'\n_____________\|__________________________________\|\n_____________36_________________________________1\n	92 -- 98	19 -- 25	-28.6527 kcal/mol	7.84855 kcal/mol	5.03645 kcal/mol	81 111@LB@ \| \|@LB@5'-UUU...UCUGG UUUG AA UAUCG...CUG-3'@LB@ UCUACA GGGGGCG AAAA AGUGGC@LB@ \|\|:\|\|\| \|\|\|\|:\|: \|\|\|\| \|\|\|\|\|\|@LB@ AGGUGU CCCCUGU UUUU UCACCG@LB@3'-UUU...ACCCG UUUA AGUUUC CG -5'@LB@ \| \|@LB@ 36 1@LB@@LB@@LB@Energy -15.77 kcal/mol@LB@Hybridization Energy -28.6527 kcal/mol@LB@Unfolding Energy - mRNA 7.84855 kcal/mol@LB@Unfolding Energy - sRNA 5.03645 kcal/mol@LB@@LB@Position - mRNA 82 -- 110@LB@Position - sRNA 1 -- 35@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 19 -- 25@LB@
147	0.01091	0.2504	b2720	hycF	-14.84	0.030299	116 -- 133	1 -- 16	formate hydrogenlyase complex iron-sulfur protein		_____________115________________134\n_____________\|__________________\|\n5'-CGA...CGUAA___________AA_____GCCGU...CAG-3'\n______________GAAGAAGAGCA__GUGGU\n______________:\|\|\|\|\|:\|\|\|\|__\|\|\|\|:\n______________UUUCUUUUCGU__CACCG\n3'-UUU...UGUAG__________________-5'\n_____________\|_________________\|\n_____________17________________1\n	120 -- 126	6 -- 12	-19.8629 kcal/mol	3.67538 kcal/mol	1.34555 kcal/mol	115 134@LB@ \| \|@LB@5'-CGA...CGUAA AA GCCGU...CAG-3'@LB@ GAAGAAGAGCA GUGGU@LB@ :\|\|\|\|\|:\|\|\|\| \|\|\|\|:@LB@ UUUCUUUUCGU CACCG@LB@3'-UUU...UGUAG -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -14.84 kcal/mol@LB@Hybridization Energy -19.8629 kcal/mol@LB@Unfolding Energy - mRNA 3.67538 kcal/mol@LB@Unfolding Energy - sRNA 1.34555 kcal/mol@LB@@LB@Position - mRNA 116 -- 133@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 6 -- 12@LB@
43	0.00215	0.1648	b4268	idnK	-13.73	0.054596	180 -- 268	1 -- 79	D-gluconate kinase thermosensitive	b3437	_____________179______________________________________________________________________________________________269\n_____________\|________________________________________________________________________________________________\|\n5'-AGG...AGGUA___U______GGA______________________U_AAAGCUUUAUU_________GU____G_______UAAA_______UUGGU____A____UGCCG...AUG-3'\n______________AGA_GAUUCA___GAUUUU______AAAUGGCGGG_G___________UUGAUGGGC__UUCA_GGAGUGG____ACAUUAA_____AGCA__GGU\n______________\|:\|_:\|::\|\|___:\|::\|\|______\|\|\|\|\|\|\|\|\|\|_\|___________\|\|\|\|\|\|\|\|\|__\|:\|\|_::\|:\|\|\|____\|\|\|\|:\|\|_____\|\|\|\|__\|\|:\n______________UUU_UUGGGU___UUGGAA______UUUACCGCCC_C___________AACUACCCG__AGGU_UUUUACC____UGUAGUU_____UCGU__CCG\n___________3'-___U______AG_______CUUGGC______________________________________G_______CC_________UCUUU____CA___-5'\n______________\|______________________________________________________________________________________________\|\n______________79_____________________________________________________________________________________________1\n	249 -- 255	15 -- 21	-57.0668 kcal/mol	16.9855 kcal/mol	26.3515 kcal/mol	179 269@LB@ \| \|@LB@5'-AGG...AGGUA U GGA U AAAGCUUUAUU GU G UAAA UUGGU A UGCCG...AUG-3'@LB@ AGA GAUUCA GAUUUU AAAUGGCGGG G UUGAUGGGC UUCA GGAGUGG ACAUUAA AGCA GGU@LB@ \|:\| :\|::\|\| :\|::\|\| \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|\|\|\|\|\| \|:\|\| ::\|:\|\|\| \|\|\|\|:\|\| \|\|\|\| \|\|:@LB@ UUU UUGGGU UUGGAA UUUACCGCCC C AACUACCCG AGGU UUUUACC UGUAGUU UCGU CCG@LB@ 3'- U AG CUUGGC G CC UCUUU CA -5'@LB@ \| \|@LB@ 79 1@LB@@LB@@LB@Energy -13.73 kcal/mol@LB@Hybridization Energy -57.0668 kcal/mol@LB@Unfolding Energy - mRNA 16.9855 kcal/mol@LB@Unfolding Energy - sRNA 26.3515 kcal/mol@LB@@LB@Position - mRNA 180 -- 268@LB@Position - sRNA 1 -- 79@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 15 -- 21@LB@
160	0.0118	0.2504	b0078	ilvH	-13.58	0.059018	151 -- 175	1 -- 25	acetolactate synthase 3 small subunit valine-sensitive		_____________150_______________________176\n_____________\|_________________________\|\n5'-GCC...UUCGC_______GAAU___UGAAAU_____UAAGC...UGA-3'\n______________GGGGGCG____GGA______GUGGU\n______________\|\|\|\|:\|:____:\|\|______\|\|\|\|:\n______________CCCCUGU____UCU______CACCG\n3'-UUU...UUUUA_______AGUU___UUUCGU_____-5'\n_____________\|________________________\|\n_____________26_______________________1\n	151 -- 157	19 -- 25	-21.9405 kcal/mol	5.84786 kcal/mol	2.51748 kcal/mol	150 176@LB@ \| \|@LB@5'-GCC...UUCGC GAAU UGAAAU UAAGC...UGA-3'@LB@ GGGGGCG GGA GUGGU@LB@ \|\|\|\|:\|: :\|\| \|\|\|\|:@LB@ CCCCUGU UCU CACCG@LB@3'-UUU...UUUUA AGUU UUUCGU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -13.58 kcal/mol@LB@Hybridization Energy -21.9405 kcal/mol@LB@Unfolding Energy - mRNA 5.84786 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 151 -- 175@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 19 -- 25@LB@
126	0.009112	0.2491	b0299	insF1	-12.99	0.078544	136 -- 158	1 -- 32	IS3 transposase B	b0372 b0541 b1026 b2089 b3558	_____________135______________________________159\n_____________\|________________________________\|\n5'-ACU...AGCUG______C_________________________UAUCC...GUC-3'\n______________GCAGAA__GGGAU______GAAGAGC__UGGC\n______________:\|\|:\|\|__\|\|\|\|:______\|\|\|:\|\|\|__\|\|\|\|\n______________UGUUUU__CCCUG______CUUUUCG__ACCG\n3'-UUU...CGAGG______AC_____UAGUUU_______UC____-5'\n_____________\|_______________________________\|\n_____________33______________________________1\n	148 -- 154	7 -- 13	-22.7833 kcal/mol	7.25293 kcal/mol	2.53929 kcal/mol	135 159@LB@ \| \|@LB@5'-ACU...AGCUG C UAUCC...GUC-3'@LB@ GCAGAA GGGAU GAAGAGC UGGC@LB@ :\|\|:\|\| \|\|\|\|: \|\|\|:\|\|\| \|\|\|\|@LB@ UGUUUU CCCUG CUUUUCG ACCG@LB@3'-UUU...CGAGG AC UAGUUU UC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -12.99 kcal/mol@LB@Hybridization Energy -22.7833 kcal/mol@LB@Unfolding Energy - mRNA 7.25293 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 136 -- 158@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 148 -- 154@LB@Position Seed - sRNA 7 -- 13@LB@
112	0.007494	0.2348	b2529	iscU	-11.51	0.152972	152 -- 183	2 -- 33	iron-sulfur cluster assembly scaffold protein		_____________151______________________________184\n_____________\|________________________________\|\n5'-UGG...AACAG___C_______CU_____UUAAUC____UC__AAUCA...GGG-3'\n______________CAU_GAAUGGG__CAUCA______GGUA__GG\n______________\|\|:_:\|\|\|\|\|\|__\|\|\|\|\|______:\|:\|__\|\|\n______________GUG_UUUACCC__GUAGU______UCGU__CC\n3'-UUU...CCGAG___U_______CU_____UUCUUU____CA__G-5'\n_____________\|________________________________\|\n_____________34_______________________________1\n	156 -- 162	23 -- 29	-21.7 kcal/mol	7.11537 kcal/mol	3.07861 kcal/mol	151 184@LB@ \| \|@LB@5'-UGG...AACAG C CU UUAAUC UC AAUCA...GGG-3'@LB@ CAU GAAUGGG CAUCA GGUA GG@LB@ \|\|: :\|\|\|\|\|\| \|\|\|\|\| :\|:\| \|\|@LB@ GUG UUUACCC GUAGU UCGU CC@LB@3'-UUU...CCGAG U CU UUCUUU CA G-5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -21.7 kcal/mol@LB@Unfolding Energy - mRNA 7.11537 kcal/mol@LB@Unfolding Energy - sRNA 3.07861 kcal/mol@LB@@LB@Position - mRNA 152 -- 183@LB@Position - sRNA 2 -- 33@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 23 -- 29@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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