Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
126	0.009112	0.2491	b0299	insF1	-12.99	0.078544	136 -- 158	1 -- 32	IS3 transposase B	b0372 b0541 b1026 b2089 b3558	_____________135______________________________159\n_____________\|________________________________\|\n5'-ACU...AGCUG______C_________________________UAUCC...GUC-3'\n______________GCAGAA__GGGAU______GAAGAGC__UGGC\n______________:\|\|:\|\|__\|\|\|\|:______\|\|\|:\|\|\|__\|\|\|\|\n______________UGUUUU__CCCUG______CUUUUCG__ACCG\n3'-UUU...CGAGG______AC_____UAGUUU_______UC____-5'\n_____________\|_______________________________\|\n_____________33______________________________1\n	148 -- 154	7 -- 13	-22.7833 kcal/mol	7.25293 kcal/mol	2.53929 kcal/mol	135 159@LB@ \| \|@LB@5'-ACU...AGCUG C UAUCC...GUC-3'@LB@ GCAGAA GGGAU GAAGAGC UGGC@LB@ :\|\|:\|\| \|\|\|\|: \|\|\|:\|\|\| \|\|\|\|@LB@ UGUUUU CCCUG CUUUUCG ACCG@LB@3'-UUU...CGAGG AC UAGUUU UC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -12.99 kcal/mol@LB@Hybridization Energy -22.7833 kcal/mol@LB@Unfolding Energy - mRNA 7.25293 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 136 -- 158@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 148 -- 154@LB@Position Seed - sRNA 7 -- 13@LB@
167	0.01216	0.2504	b1013	rutR	-12.90	0.082087	181 -- 218	3 -- 35	rut operon transcriptional repressor for	b0796	_____________180________________________________________219\n_____________\|__________________________________________\|\n5'-CAA...GCCAA_______________GAGCGCAUGACG_____C_________AAAAC...AAU-3'\n______________UCUACAAGA_GGGGA____________CAAGG____GCAGUG\n______________\|\|:\|\|\|\|:\|_\|\|\|\|\|____________\|\|\|:\|____\|\|\|\|\|\|\n______________AGGUGUUUU_CCCCU____________GUUUC____CGUCAC\n3'-UUU...ACCCG_________A_____GUA______________UUUU______CG-5'\n_____________\|__________________________________________\|\n_____________36_________________________________________2\n	181 -- 187	29 -- 35	-29.1146 kcal/mol	11.1798 kcal/mol	5.03644 kcal/mol	180 219@LB@ \| \|@LB@5'-CAA...GCCAA GAGCGCAUGACG C AAAAC...AAU-3'@LB@ UCUACAAGA GGGGA CAAGG GCAGUG@LB@ \|\|:\|\|\|\|:\| \|\|\|\|\| \|\|\|:\| \|\|\|\|\|\|@LB@ AGGUGUUUU CCCCU GUUUC CGUCAC@LB@3'-UUU...ACCCG A GUA UUUU CG-5'@LB@ \| \|@LB@ 36 2@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -29.1146 kcal/mol@LB@Unfolding Energy - mRNA 11.1798 kcal/mol@LB@Unfolding Energy - sRNA 5.03644 kcal/mol@LB@@LB@Position - mRNA 181 -- 218@LB@Position - sRNA 3 -- 35@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 29 -- 35@LB@
6	9.344e-05	0.05465	b2215	ompC	-14.72	0.032428	133 -- 159	1 -- 28	outer membrane porin protein C	b0929 b0241 b1377	_____________132____________________________160\n_____________\|______________________________\|\n5'-AUC...UGAUU____A___UUA_____UAU___________AUAAA...AUC-3'\n______________AAUG_GGG___AUCAG______GCAGUGGC\n______________\|\|\|\|_\|\|\|___\|\|\|\|:______\|\|\|\|\|\|\|\|\n______________UUAC_CCC___UAGUU______CGUCACCG\n3'-UUU...GUGUU________UG______UCUUUU________-5'\n_____________\|_____________________________\|\n_____________29____________________________1\n	153 -- 159	1 -- 7	-26.8714 kcal/mol	9.63393 kcal/mol	2.519 kcal/mol	132 160@LB@ \| \|@LB@5'-AUC...UGAUU A UUA UAU AUAAA...AUC-3'@LB@ AAUG GGG AUCAG GCAGUGGC@LB@ \|\|\|\| \|\|\| \|\|\|\|: \|\|\|\|\|\|\|\|@LB@ UUAC CCC UAGUU CGUCACCG@LB@3'-UUU...GUGUU UG UCUUUU -5'@LB@ \| \|@LB@ 29 1@LB@@LB@@LB@Energy -14.72 kcal/mol@LB@Hybridization Energy -26.8714 kcal/mol@LB@Unfolding Energy - mRNA 9.63393 kcal/mol@LB@Unfolding Energy - sRNA 2.519 kcal/mol@LB@@LB@Position - mRNA 133 -- 159@LB@Position - sRNA 1 -- 28@LB@Position Seed - mRNA 153 -- 159@LB@Position Seed - sRNA 1 -- 7@LB@
152	0.01123	0.2504	b1872	torZ	-17.40	0.006326	76 -- 107	1 -- 34	trimethylamine N-oxide reductase system III catalytic subunit	b0997 b3551	_____________75___________________________________108\n_____________\|____________________________________\|\n5'-ACG...CAUUG___AA_________________CC_________A__GAAAA...AGA-3'\n______________CCA__GGAAUGGGGGCA_CGAA____AGCA_UG_GC\n______________\|\|\|__::\|\|\|\|\|\|\|:\|\|_\|:\|\|____\|\|\|\|_\|\|_\|\|\n______________GGU__UUUUACCCCUGU_GUUU____UCGU_AC_CG\n3'-UUU...CCCGA___G_____________A____CUUU____C_____-5'\n_____________\|___________________________________\|\n_____________35__________________________________1\n	86 -- 92	19 -- 25	-29.6219 kcal/mol	7.95271 kcal/mol	4.26884 kcal/mol	75 108@LB@ \| \|@LB@5'-ACG...CAUUG AA CC A GAAAA...AGA-3'@LB@ CCA GGAAUGGGGGCA CGAA AGCA UG GC@LB@ \|\|\| ::\|\|\|\|\|\|\|:\|\| \|:\|\| \|\|\|\| \|\| \|\|@LB@ GGU UUUUACCCCUGU GUUU UCGU AC CG@LB@3'-UUU...CCCGA G A CUUU C -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -17.40 kcal/mol@LB@Hybridization Energy -29.6219 kcal/mol@LB@Unfolding Energy - mRNA 7.95271 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 76 -- 107@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 19 -- 25@LB@
19	0.000738	0.1243	b1508	hipB	-18.00	0.004180	266 -- 295	1 -- 32	antitoxin of HipAB toxin-antitoxin system	b1299 b1434	_____________265________________________________296\n_____________\|__________________________________\|\n5'-AUG...CGCCA_________CU___________C___________GAAAA-3'\n______________GCAAAAUGG__GGACG_CAGAG__GAGC__UGGC\n______________:\|\|\|\|\|\|\|\|__\|\|\|\|:_\|\|:\|\|__:\|\|\|__\|\|\|\|\n______________UGUUUUACC__CCUGU_GUUUC__UUCG__ACCG\n3'-UUU...CGAGG________________A_____UU____UC____-5'\n_____________\|_________________________________\|\n_____________33________________________________1\n	268 -- 274	24 -- 30	-30.0826 kcal/mol	9.54799 kcal/mol	2.53929 kcal/mol	265 296@LB@ \| \|@LB@5'-AUG...CGCCA CU C GAAAA-3'@LB@ GCAAAAUGG GGACG CAGAG GAGC UGGC@LB@ :\|\|\|\|\|\|\|\| \|\|\|\|: \|\|:\|\| :\|\|\| \|\|\|\|@LB@ UGUUUUACC CCUGU GUUUC UUCG ACCG@LB@3'-UUU...CGAGG A UU UC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -30.0826 kcal/mol@LB@Unfolding Energy - mRNA 9.54799 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 266 -- 295@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 24 -- 30@LB@
24	0.0009771	0.1361	b4295	yjhU	-16.20	0.013710	198 -- 226	5 -- 30	putative DNA-binding transcriptional regulator; KpLE2 phage-like element	b1512	_____________197___________________________227\n_____________\|_____________________________\|\n5'-GUA...AUACA_______AUA__G______C___C_____CCUGU...UUG-3'\n______________AAAGUGG___GA_AUCAAA_AAA_AGCAG\n______________\|\|\|:\|\|\|___\|\|_\|\|\|\|\|\|_\|\|\|_\|\|\|\|\|\n______________UUUUACC___CU_UAGUUU_UUU_UCGUC\n3'-UUU...AGGUG_______C____G______C_________ACCG-5'\n_____________\|_____________________________\|\n_____________31____________________________4\n	198 -- 204	24 -- 30	-21.4023 kcal/mol	2.69702 kcal/mol	2.50278 kcal/mol	197 227@LB@ \| \|@LB@5'-GUA...AUACA AUA G C C CCUGU...UUG-3'@LB@ AAAGUGG GA AUCAAA AAA AGCAG@LB@ \|\|\|:\|\|\| \|\| \|\|\|\|\|\| \|\|\| \|\|\|\|\|@LB@ UUUUACC CU UAGUUU UUU UCGUC@LB@3'-UUU...AGGUG C G C ACCG-5'@LB@ \| \|@LB@ 31 4@LB@@LB@@LB@Energy -16.20 kcal/mol@LB@Hybridization Energy -21.4023 kcal/mol@LB@Unfolding Energy - mRNA 2.69702 kcal/mol@LB@Unfolding Energy - sRNA 2.50278 kcal/mol@LB@@LB@Position - mRNA 198 -- 226@LB@Position - sRNA 5 -- 30@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 24 -- 30@LB@
67	0.003548	0.1858	b4412	hokC	-18.22	0.003564	16 -- 36	7 -- 32	toxic membrane protein small	b1562 b0018	_____________15_________________________37\n_____________\|__________________________\|\n5'-AUU...AUAAA__________________________GAUAU...ACA-3'\n______________ACAAAAUGGG____UUAAAGAGA_GC\n______________\|\|\|\|\|\|\|\|\|\|____\|:\|\|\|\|\|:\|_\|\|\n______________UGUUUUACCC____AGUUUCUUU_CG\n3'-UUU...CGAGG__________CUGU_________U__UCACCG-5'\n_____________\|__________________________\|\n_____________33_________________________6\n	28 -- 34	10 -- 16	-22.2189 kcal/mol	1.46836 kcal/mol	2.53437 kcal/mol	15 37@LB@ \| \|@LB@5'-AUU...AUAAA GAUAU...ACA-3'@LB@ ACAAAAUGGG UUAAAGAGA GC@LB@ \|\|\|\|\|\|\|\|\|\| \|:\|\|\|\|\|:\| \|\|@LB@ UGUUUUACCC AGUUUCUUU CG@LB@3'-UUU...CGAGG CUGU U UCACCG-5'@LB@ \| \|@LB@ 33 6@LB@@LB@@LB@Energy -18.22 kcal/mol@LB@Hybridization Energy -22.2189 kcal/mol@LB@Unfolding Energy - mRNA 1.46836 kcal/mol@LB@Unfolding Energy - sRNA 2.53437 kcal/mol@LB@@LB@Position - mRNA 16 -- 36@LB@Position - sRNA 7 -- 32@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 10 -- 16@LB@
172	0.01269	0.2504	b0571	cusR	-10.48	0.230388	14 -- 48	1 -- 33	response regulator in two-component regulatory system with CusS	b1969	_____________13___________________________________49\n_____________\|____________________________________\|\n5'-AGC...GGCCA__________CA__A___UAC__________UA___UCAUA...GGC-3'\n______________CACAAAAUGG__GA_GUU___AAGGAGA_CA__GGC\n______________\|\|\|\|\|\|\|\|\|\|__\|\|_:\|:___\|\|\|:\|:\|_\|\|__\|\|\|\n______________GUGUUUUACC__CU_UAG___UUCUUUU_GU__CCG\n3'-UUU...CCGAG__________C___G___U_________C__CA___-5'\n_____________\|___________________________________\|\n_____________34__________________________________1\n	35 -- 41	9 -- 15	-22.821 kcal/mol	9.26274 kcal/mol	3.07861 kcal/mol	13 49@LB@ \| \|@LB@5'-AGC...GGCCA CA A UAC UA UCAUA...GGC-3'@LB@ CACAAAAUGG GA GUU AAGGAGA CA GGC@LB@ \|\|\|\|\|\|\|\|\|\| \|\| :\|: \|\|\|:\|:\| \|\| \|\|\|@LB@ GUGUUUUACC CU UAG UUCUUUU GU CCG@LB@3'-UUU...CCGAG C G U C CA -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -22.821 kcal/mol@LB@Unfolding Energy - mRNA 9.26274 kcal/mol@LB@Unfolding Energy - sRNA 3.07861 kcal/mol@LB@@LB@Position - mRNA 14 -- 48@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 9 -- 15@LB@
141	0.01037	0.2504	b2161	nupX	-15.31	0.023296	54 -- 80	1 -- 26	nucleoside permease	b2164	_____________53____________________________81\n_____________\|_____________________________\|\n5'-UUG...GGUUC_______UA_________U_______C__GGCUA...GCG-3'\n______________UGGGGAU__GUCGAAGAG__GC_GUG_GC\n______________\|\|\|\|\|\|:__:\|\|:\|\|\|\|:__\|\|_\|\|\|_\|\|\n______________ACCCCUG__UAGUUUCUU__CG_CAC_CG\n3'-UUU...GUUUU__________________UU__U______-5'\n_____________\|____________________________\|\n_____________27___________________________1\n	65 -- 71	11 -- 17	-27.7009 kcal/mol	9.87339 kcal/mol	2.51842 kcal/mol	53 81@LB@ \| \|@LB@5'-UUG...GGUUC UA U C GGCUA...GCG-3'@LB@ UGGGGAU GUCGAAGAG GC GUG GC@LB@ \|\|\|\|\|\|: :\|\|:\|\|\|\|: \|\| \|\|\| \|\|@LB@ ACCCCUG UAGUUUCUU CG CAC CG@LB@3'-UUU...GUUUU UU U -5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -15.31 kcal/mol@LB@Hybridization Energy -27.7009 kcal/mol@LB@Unfolding Energy - mRNA 9.87339 kcal/mol@LB@Unfolding Energy - sRNA 2.51842 kcal/mol@LB@@LB@Position - mRNA 54 -- 80@LB@Position - sRNA 1 -- 26@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 11 -- 17@LB@
97	0.005972	0.2161	b0768	ybhD	-15.34	0.022873	51 -- 78	6 -- 32	putative DNA-binding transcriptional regulator	b2409	_____________50___________________________79\n_____________\|____________________________\|\n5'-CAG...UUAUU____U____UU__________C______UCUCU...CAU-3'\n______________GCGA_AUGG__ACAUUAAGGG_AAAGCA\n______________:\|:\|_\|\|\|\|__\|\|\|\|:\|\|:\|:_\|\|\|\|\|\|\n______________UGUU_UACC__UGUAGUUUCU_UUUCGU\n3'-UUU...CGAGG____U____CC_________________CACCG-5'\n_____________\|____________________________\|\n_____________33___________________________5\n	64 -- 70	13 -- 19	-24.2506 kcal/mol	6.37495 kcal/mol	2.53457 kcal/mol	50 79@LB@ \| \|@LB@5'-CAG...UUAUU U UU C UCUCU...CAU-3'@LB@ GCGA AUGG ACAUUAAGGG AAAGCA@LB@ :\|:\| \|\|\|\| \|\|\|\|:\|\|:\|: \|\|\|\|\|\|@LB@ UGUU UACC UGUAGUUUCU UUUCGU@LB@3'-UUU...CGAGG U CC CACCG-5'@LB@ \| \|@LB@ 33 5@LB@@LB@@LB@Energy -15.34 kcal/mol@LB@Hybridization Energy -24.2506 kcal/mol@LB@Unfolding Energy - mRNA 6.37495 kcal/mol@LB@Unfolding Energy - sRNA 2.53457 kcal/mol@LB@@LB@Position - mRNA 51 -- 78@LB@Position - sRNA 6 -- 32@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 13 -- 19@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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