Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
120	0.00842	0.2462	b1068	yceM	-12.88	0.082928	190 -- 205	9 -- 24	putative oxidoreductase		_____________189______________206\n_____________\|________________\|\n5'-GCG...CAUCU____G_______U___AAAAU...GAG-3'\n______________GGGA_AUUAAAG_GAA\n______________\|\|\|\|_\|\|:\|\|\|\|_:\|\|\n______________CCCU_UAGUUUC_UUU\n3'-UUU...UUUAC____G_______U___CGUCACCG-5'\n_____________\|________________\|\n_____________25_______________8\n	195 -- 201	13 -- 19	-15.5519 kcal/mol	2.0153 kcal/mol	0.659805 kcal/mol	189 206@LB@ \| \|@LB@5'-GCG...CAUCU G U AAAAU...GAG-3'@LB@ GGGA AUUAAAG GAA@LB@ \|\|\|\| \|\|:\|\|\|\| :\|\|@LB@ CCCU UAGUUUC UUU@LB@3'-UUU...UUUAC G U CGUCACCG-5'@LB@ \| \|@LB@ 25 8@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -15.5519 kcal/mol@LB@Unfolding Energy - mRNA 2.0153 kcal/mol@LB@Unfolding Energy - sRNA 0.659805 kcal/mol@LB@@LB@Position - mRNA 190 -- 205@LB@Position - sRNA 9 -- 24@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 13 -- 19@LB@
100	0.006296	0.2188	b1321	ycjX	-13.69	0.055797	38 -- 45	18 -- 25	DUF463 family protein puatative P-loop NTPase		_____________37_______46\n_____________\|________\|\n5'-UGA...UAGUA________AGGUC...CAG-3'\n______________GGGGACAU\n______________\|\|\|\|\|\|\|\|\n______________CCCCUGUA\n3'-UUU...UUUUA________GUUUC...CCG-5'\n_____________\|________\|\n_____________26_______17\n	39 -- 45	18 -- 24	-15.5505 kcal/mol	1.21152 kcal/mol	0.65218 kcal/mol	37 46@LB@ \| \|@LB@5'-UGA...UAGUA AGGUC...CAG-3'@LB@ GGGGACAU@LB@ \|\|\|\|\|\|\|\|@LB@ CCCCUGUA@LB@3'-UUU...UUUUA GUUUC...CCG-5'@LB@ \| \|@LB@ 26 17@LB@@LB@@LB@Energy -13.69 kcal/mol@LB@Hybridization Energy -15.5505 kcal/mol@LB@Unfolding Energy - mRNA 1.21152 kcal/mol@LB@Unfolding Energy - sRNA 0.65218 kcal/mol@LB@@LB@Position - mRNA 38 -- 45@LB@Position - sRNA 18 -- 25@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 18 -- 24@LB@
104	0.006761	0.2281	b1671	ydhX	-13.25	0.069212	145 -- 167	2 -- 25	putative 4Fe-4S ferridoxin-type protein; FNR Nar NarP-regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex	b4072	_____________144_______________________168\n_____________\|_________________________\|\n5'-CAC...UUUCA___A_______U___U_________ACAGU...AGG-3'\n______________GGG_GAUGUCA_GGA__AG_GGUGG\n______________\|\|\|_\|\|::\|\|\|_:\|\|__\|\|_:\|\|\|\|\n______________CCC_CUGUAGU_UCU__UC_UCACC\n3'-UUU...UUUUA___________U___UU__G_____G-5'\n_____________\|_________________________\|\n_____________26________________________1\n	149 -- 155	16 -- 22	-23.0825 kcal/mol	7.31216 kcal/mol	2.51746 kcal/mol	144 168@LB@ \| \|@LB@5'-CAC...UUUCA A U U ACAGU...AGG-3'@LB@ GGG GAUGUCA GGA AG GGUGG@LB@ \|\|\| \|\|::\|\|\| :\|\| \|\| :\|\|\|\|@LB@ CCC CUGUAGU UCU UC UCACC@LB@3'-UUU...UUUUA U UU G G-5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -13.25 kcal/mol@LB@Hybridization Energy -23.0825 kcal/mol@LB@Unfolding Energy - mRNA 7.31216 kcal/mol@LB@Unfolding Energy - sRNA 2.51746 kcal/mol@LB@@LB@Position - mRNA 145 -- 167@LB@Position - sRNA 2 -- 25@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 16 -- 22@LB@
75	0.00423	0.1979	b1917	yecC	-10.99	0.188691	288 -- 298	1 -- 11	putative ABC transporter ATPase		_____________287_________299\n_____________\|___________\|\n5'-CUG...CUGGC___________AA-3'\n______________GAAGUGGUGGC\n______________:\|\|\|::\|\|\|\|\|\n______________UUUCGUCACCG\n3'-UUU...UUUCU___________-5'\n_____________\|__________\|\n_____________12_________1\n	292 -- 298	1 -- 7	-16.6045 kcal/mol	4.33348 kcal/mol	1.27751 kcal/mol	287 299@LB@ \| \|@LB@5'-CUG...CUGGC AA-3'@LB@ GAAGUGGUGGC@LB@ :\|\|\|::\|\|\|\|\|@LB@ UUUCGUCACCG@LB@3'-UUU...UUUCU -5'@LB@ \| \|@LB@ 12 1@LB@@LB@@LB@Energy -10.99 kcal/mol@LB@Hybridization Energy -16.6045 kcal/mol@LB@Unfolding Energy - mRNA 4.33348 kcal/mol@LB@Unfolding Energy - sRNA 1.27751 kcal/mol@LB@@LB@Position - mRNA 288 -- 298@LB@Position - sRNA 1 -- 11@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 1 -- 7@LB@
92	0.005693	0.2147	b2099	yegU	-12.49	0.099484	183 -- 197	7 -- 22	ADP-ribosylglycohydrolase family protein		_____________182______________198\n_____________\|________________\|\n5'-GUG...GCGUU________________UAAAU...CAC-3'\n______________GACA_CAAGGGGAAGU\n______________\|\|\|\|_\|\|\|:\|::\|\|\|:\n______________CUGU_GUUUCUUUUCG\n3'-UUU...UACCC____A___________UCACCG-5'\n_____________\|________________\|\n_____________23_______________6\n	191 -- 197	7 -- 13	-15.6094 kcal/mol	2.53245 kcal/mol	0.590875 kcal/mol	182 198@LB@ \| \|@LB@5'-GUG...GCGUU UAAAU...CAC-3'@LB@ GACA CAAGGGGAAGU@LB@ \|\|\|\| \|\|\|:\|::\|\|\|:@LB@ CUGU GUUUCUUUUCG@LB@3'-UUU...UACCC A UCACCG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -12.49 kcal/mol@LB@Hybridization Energy -15.6094 kcal/mol@LB@Unfolding Energy - mRNA 2.53245 kcal/mol@LB@Unfolding Energy - sRNA 0.590875 kcal/mol@LB@@LB@Position - mRNA 183 -- 197@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 7 -- 13@LB@
182	0.01317	0.2504	b2101	yegW	-15.40	0.022099	126 -- 162	3 -- 28	putative DNA-binding transcriptional regulator		_____________125______________________________________163\n_____________\|________________________________________\|\n5'-CGG...UCGUU_________________AACGCCUUAAAUAA_________AUAUA...AAA-3'\n______________GAUGGG___AUUAAAGA______________GAAGCAGUG\n______________:\|\|\|\|\|___\|\|:\|\|\|\|\|______________:\|\|\|\|\|\|\|\|\n______________UUACCC___UAGUUUCU______________UUUCGUCAC\n3'-UUU...GUGUU______CUG_______________________________CG-5'\n_____________\|________________________________________\|\n_____________29_______________________________________2\n	156 -- 162	3 -- 9	-24.3736 kcal/mol	6.45453 kcal/mol	2.51813 kcal/mol	125 163@LB@ \| \|@LB@5'-CGG...UCGUU AACGCCUUAAAUAA AUAUA...AAA-3'@LB@ GAUGGG AUUAAAGA GAAGCAGUG@LB@ :\|\|\|\|\| \|\|:\|\|\|\|\| :\|\|\|\|\|\|\|\|@LB@ UUACCC UAGUUUCU UUUCGUCAC@LB@3'-UUU...GUGUU CUG CG-5'@LB@ \| \|@LB@ 29 2@LB@@LB@@LB@Energy -15.40 kcal/mol@LB@Hybridization Energy -24.3736 kcal/mol@LB@Unfolding Energy - mRNA 6.45453 kcal/mol@LB@Unfolding Energy - sRNA 2.51813 kcal/mol@LB@@LB@Position - mRNA 126 -- 162@LB@Position - sRNA 3 -- 28@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 3 -- 9@LB@
197	0.0142	0.2529	b2295	yfbV	-14.79	0.031139	250 -- 276	1 -- 25	UPF0208 family inner membrane protein		_____________249_________________________277\n_____________\|___________________________\|\n5'-AUG...UCGCC_______G__UUACUC____AC_____CGCUG...CGG-3'\n______________GGGGACA_CA______AAAG__GUGGC\n______________\|\|\|\|\|\|\|_\|\|______\|\|\|\|__\|\|\|\|\|\n______________CCCCUGU_GU______UUUC__CACCG\n3'-UUU...UUUUA_______A__UUCU______GU_____-5'\n_____________\|__________________________\|\n_____________26_________________________1\n	250 -- 256	19 -- 25	-25.4481 kcal/mol	8.13822 kcal/mol	2.51748 kcal/mol	249 277@LB@ \| \|@LB@5'-AUG...UCGCC G UUACUC AC CGCUG...CGG-3'@LB@ GGGGACA CA AAAG GUGGC@LB@ \|\|\|\|\|\|\| \|\| \|\|\|\| \|\|\|\|\|@LB@ CCCCUGU GU UUUC CACCG@LB@3'-UUU...UUUUA A UUCU GU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -14.79 kcal/mol@LB@Hybridization Energy -25.4481 kcal/mol@LB@Unfolding Energy - mRNA 8.13822 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 250 -- 276@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 19 -- 25@LB@
71	0.003993	0.1953	b2347	yfdC	-11.68	0.141997	251 -- 269	1 -- 17	putative inner membrane protein		_____________250__________________270\n_____________\|____________________\|\n5'-GCA...GAGAG_______AAA______C___AAAAA...UCC-3'\n______________CGAAGAA___GAGC_G_GGC\n______________\|:\|\|\|\|\|___:\|\|\|_\|_\|\|\|\n______________GUUUCUU___UUCG_C_CCG\n3'-UUU...CUGUA______________U_A___-5'\n_____________\|___________________\|\n_____________18__________________1\n	251 -- 257	11 -- 17	-16.1242 kcal/mol	3.00377 kcal/mol	1.43853 kcal/mol	250 270@LB@ \| \|@LB@5'-GCA...GAGAG AAA C AAAAA...UCC-3'@LB@ CGAAGAA GAGC G GGC@LB@ \|:\|\|\|\|\| :\|\|\| \| \|\|\|@LB@ GUUUCUU UUCG C CCG@LB@3'-UUU...CUGUA U A -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -16.1242 kcal/mol@LB@Unfolding Energy - mRNA 3.00377 kcal/mol@LB@Unfolding Energy - sRNA 1.43853 kcal/mol@LB@@LB@Position - mRNA 251 -- 269@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 251 -- 257@LB@Position Seed - sRNA 11 -- 17@LB@
84	0.005241	0.2147	b2513	yfgM	-12.13	0.116945	176 -- 204	2 -- 26	ancillary SecYEG translocon subunit; putative anti-RcsB factor		_____________175___________________________205\n_____________\|_____________________________\|\n5'-GGC...GUUAC____UUAAGG_________G_CAGC____AAAUU...UUG-3'\n______________UGGG______AAGGAGAAG_A____GUGG\n______________\|\|\|\|______\|\|:\|\|:\|\|\|_\|____\|\|\|\|\n______________ACCC______UUUCUUUUC_U____CACC\n3'-UUU...GUUUU____CUGUAG_________G_________G-5'\n_____________\|_____________________________\|\n_____________27____________________________1\n	188 -- 194	8 -- 14	-19.1762 kcal/mol	4.53021 kcal/mol	2.5184 kcal/mol	175 205@LB@ \| \|@LB@5'-GGC...GUUAC UUAAGG G CAGC AAAUU...UUG-3'@LB@ UGGG AAGGAGAAG A GUGG@LB@ \|\|\|\| \|\|:\|\|:\|\|\| \| \|\|\|\|@LB@ ACCC UUUCUUUUC U CACC@LB@3'-UUU...GUUUU CUGUAG G G-5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -12.13 kcal/mol@LB@Hybridization Energy -19.1762 kcal/mol@LB@Unfolding Energy - mRNA 4.53021 kcal/mol@LB@Unfolding Energy - sRNA 2.5184 kcal/mol@LB@@LB@Position - mRNA 176 -- 204@LB@Position - sRNA 2 -- 26@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 8 -- 14@LB@
85	0.0054	0.2147	b4461	yfjD	-16.56	0.010978	60 -- 89	2 -- 27	UPF0053 family inner membrane protein		_____________59______________________________90\n_____________\|_______________________________\|\n5'-GAA...CUGCU___________AUCAU______U________ACGCC...UCA-3'\n______________GUGGGGACAUU_____GAAGGA_GGC_GUGG\n______________:\|\|\|\|\|\|\|\|\|:_____:\|\|\|:\|_:\|\|_\|\|\|\|\n______________UACCCCUGUAG_____UUUCUU_UCG_CACC\n3'-UUU...UGUUU______________________U___U____G-5'\n_____________\|_______________________________\|\n_____________28______________________________1\n	64 -- 70	17 -- 23	-31.7913 kcal/mol	12.7137 kcal/mol	2.51866 kcal/mol	59 90@LB@ \| \|@LB@5'-GAA...CUGCU AUCAU U ACGCC...UCA-3'@LB@ GUGGGGACAUU GAAGGA GGC GUGG@LB@ :\|\|\|\|\|\|\|\|\|: :\|\|\|:\| :\|\| \|\|\|\|@LB@ UACCCCUGUAG UUUCUU UCG CACC@LB@3'-UUU...UGUUU U U G-5'@LB@ \| \|@LB@ 28 1@LB@@LB@@LB@Energy -16.56 kcal/mol@LB@Hybridization Energy -31.7913 kcal/mol@LB@Unfolding Energy - mRNA 12.7137 kcal/mol@LB@Unfolding Energy - sRNA 2.51866 kcal/mol@LB@@LB@Position - mRNA 60 -- 89@LB@Position - sRNA 2 -- 27@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 17 -- 23@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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