Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
113	0.00768	0.2368	b1923	fliC	-14.29	0.040892	38 -- 54	4 -- 25	flagellar filament structural protein (flagellin)	_____________37_____________________55\n_____________\|______________________\|\n5'-GUU...AAUAA______________________AUUUC...CUU-3'\n______________GGGG____CAGAGAAAAG_AGU\n______________\|\|\|\|____\|\|:\|\|\|\|\|\|\|_\|\|\|\n______________CCCC____GUUUCUUUUC_UCA\n3'-UUU...UUUUA____UGUA__________G___CCG-5'\n_____________\|______________________\|\n_____________26_____________________3\n	45 -- 51	8 -- 14	-19.9416 kcal/mol	3.14092 kcal/mol	2.51306 kcal/mol	37 55@LB@ \| \|@LB@5'-GUU...AAUAA AUUUC...CUU-3'@LB@ GGGG CAGAGAAAAG AGU@LB@ \|\|\|\| \|\|:\|\|\|\|\|\|\| \|\|\|@LB@ CCCC GUUUCUUUUC UCA@LB@3'-UUU...UUUUA UGUA G CCG-5'@LB@ \| \|@LB@ 26 3@LB@@LB@@LB@Energy -14.29 kcal/mol@LB@Hybridization Energy -19.9416 kcal/mol@LB@Unfolding Energy - mRNA 3.14092 kcal/mol@LB@Unfolding Energy - sRNA 2.51306 kcal/mol@LB@@LB@Position - mRNA 38 -- 54@LB@Position - sRNA 4 -- 25@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 8 -- 14@LB@
145	0.01066	0.2504	b2153	folE	-11.37	0.162147	44 -- 68	1 -- 21	GTP cyclohydrolase I	_____________43________________________69\n_____________\|_________________________\|\n5'-CUG...UUUUA_____UGCU__U_________C___AAUUA...ACG-3'\n______________ACAUC____GA_GAAAGGCAG_GGC\n______________\|\|\|\|\|____:\|_\|\|\|\|:\|\|\|\|_\|\|\|\n______________UGUAG____UU_CUUUUCGUC_CCG\n3'-UUU...ACCCC___________U_________A___-5'\n_____________\|________________________\|\n_____________22_______________________1\n	58 -- 64	5 -- 11	-23.5216 kcal/mol	10.6442 kcal/mol	1.51103 kcal/mol	43 69@LB@ \| \|@LB@5'-CUG...UUUUA UGCU U C AAUUA...ACG-3'@LB@ ACAUC GA GAAAGGCAG GGC@LB@ \|\|\|\|\| :\| \|\|\|\|:\|\|\|\| \|\|\|@LB@ UGUAG UU CUUUUCGUC CCG@LB@3'-UUU...ACCCC U A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -11.37 kcal/mol@LB@Hybridization Energy -23.5216 kcal/mol@LB@Unfolding Energy - mRNA 10.6442 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 44 -- 68@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 5 -- 11@LB@
56	0.002838	0.1778	b3371	frlB	-17.11	0.007704	196 -- 228	2 -- 32	fructoselysine-6-P-deglycase	_____________195________________________________229\n_____________\|__________________________________\|\n5'-ACU...AGAAA________UU_________U________CC____ACUUU...ACG-3'\n______________AUAAAAUG__GGAUAUUGA__AAAAGCA__GUGG\n______________\|:\|\|\|\|\|\|__\|\|\|:\|\|::\|__\|\|\|\|\|\|\|__\|\|\|\|\n______________UGUUUUAC__CCUGUAGUU__UUUUCGU__CACC\n3'-UUU...CGAGG________C__________UC_____________G-5'\n_____________\|__________________________________\|\n_____________33_________________________________1\n	216 -- 222	6 -- 12	-25.2618 kcal/mol	5.61622 kcal/mol	2.53929 kcal/mol	195 229@LB@ \| \|@LB@5'-ACU...AGAAA UU U CC ACUUU...ACG-3'@LB@ AUAAAAUG GGAUAUUGA AAAAGCA GUGG@LB@ \|:\|\|\|\|\|\| \|\|\|:\|\|::\| \|\|\|\|\|\|\| \|\|\|\|@LB@ UGUUUUAC CCUGUAGUU UUUUCGU CACC@LB@3'-UUU...CGAGG C UC G-5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -17.11 kcal/mol@LB@Hybridization Energy -25.2618 kcal/mol@LB@Unfolding Energy - mRNA 5.61622 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 196 -- 228@LB@Position - sRNA 2 -- 32@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 6 -- 12@LB@
155	0.01136	0.2504	b2168	fruK	-7.75	0.545043	91 -- 105	7 -- 22	fructose-1-phosphate kinase	_____________90_______________106\n_____________\|________________\|\n5'-AAA...CACUU________________CAUCG...CCA-3'\n______________GGCGUU_AAGAAAGGU\n______________\|:\|:\|:_\|\|\|\|\|\|:\|:\n______________CUGUAG_UUCUUUUCG\n3'-UUU...UACCC______U_________UCACCG-5'\n_____________\|________________\|\n_____________23_______________6\n	99 -- 105	7 -- 13	-14.0922 kcal/mol	5.75103 kcal/mol	0.590875 kcal/mol	90 106@LB@ \| \|@LB@5'-AAA...CACUU CAUCG...CCA-3'@LB@ GGCGUU AAGAAAGGU@LB@ \|:\|:\|: \|\|\|\|\|\|:\|:@LB@ CUGUAG UUCUUUUCG@LB@3'-UUU...UACCC U UCACCG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -7.75 kcal/mol@LB@Hybridization Energy -14.0922 kcal/mol@LB@Unfolding Energy - mRNA 5.75103 kcal/mol@LB@Unfolding Energy - sRNA 0.590875 kcal/mol@LB@@LB@Position - mRNA 91 -- 105@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 7 -- 13@LB@
186	0.01333	0.2514	b0084	ftsI	-11.80	0.135121	196 -- 214	1 -- 22	transpeptidase involved in septal peptidoglycan synthesis; penicillin-binding protein 3	_____________195____________________215\n_____________\|______________________\|\n5'-GUU...AACGC_______U__________C___GAAAA...UGG-3'\n______________GACG_CA___GAAAGCAG_GGC\n______________\|\|\|:_\|\|___:\|\|\|\|\|\|\|_\|\|\|\n______________CUGU_GU___UUUUCGUC_CCG\n3'-UUU...UACCC____A__UUC________A___-5'\n_____________\|_____________________\|\n_____________23____________________1\n	204 -- 210	5 -- 11	-21.3841 kcal/mol	8.04628 kcal/mol	1.54023 kcal/mol	195 215@LB@ \| \|@LB@5'-GUU...AACGC U C GAAAA...UGG-3'@LB@ GACG CA GAAAGCAG GGC@LB@ \|\|\|: \|\| :\|\|\|\|\|\|\| \|\|\|@LB@ CUGU GU UUUUCGUC CCG@LB@3'-UUU...UACCC A UUC A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -21.3841 kcal/mol@LB@Unfolding Energy - mRNA 8.04628 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 196 -- 214@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 5 -- 11@LB@
87	0.005508	0.2147	b0083	ftsL	-13.20	0.070894	129 -- 146	1 -- 23	membrane bound cell division leucine zipper septum protein	_____________128_____________________147\n_____________\|_______________________\|\n5'-CGU...AUGCC_______________________UGAGA...UGC-3'\n______________GGGC___GAAGAAGAG__GUGGC\n______________\|\|:\|___:\|\|\|\|\|:\|\|__\|\|\|\|\|\n______________CCUG___UUUCUUUUC__CACCG\n3'-UUU...UUACC____UAG_________GU_____-5'\n_____________\|______________________\|\n_____________24_____________________1\n	135 -- 141	8 -- 14	-20.4792 kcal/mol	5.69631 kcal/mol	1.57928 kcal/mol	128 147@LB@ \| \|@LB@5'-CGU...AUGCC UGAGA...UGC-3'@LB@ GGGC GAAGAAGAG GUGGC@LB@ \|\|:\| :\|\|\|\|\|:\|\| \|\|\|\|\|@LB@ CCUG UUUCUUUUC CACCG@LB@3'-UUU...UUACC UAG GU -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -13.20 kcal/mol@LB@Hybridization Energy -20.4792 kcal/mol@LB@Unfolding Energy - mRNA 5.69631 kcal/mol@LB@Unfolding Energy - sRNA 1.57928 kcal/mol@LB@@LB@Position - mRNA 129 -- 146@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 8 -- 14@LB@
37	0.001785	0.1648	b3933	ftsN	-15.64	0.019200	24 -- 52	1 -- 29	essential cell division protein	_____________23______________________________53\n_____________\|_______________________________\|\n5'-AGC...UGAUC_____CA______A__CGUU___________UCUCG...CUC-3'\n______________AAAUG__GGGGCA_CA______GGCAGUGGC\n______________\|\|\|\|\|__\|\|\|:\|\|_\|\|______:\|\|\|\|\|\|\|\|\n______________UUUAC__CCCUGU_GU______UCGUCACCG\n3'-UUU...GGUGU_____________A__UUCUUU_________-5'\n_____________\|______________________________\|\n_____________30_____________________________1\n	46 -- 52	1 -- 7	-29.0551 kcal/mol	10.8938 kcal/mol	2.51907 kcal/mol	23 53@LB@ \| \|@LB@5'-AGC...UGAUC CA A CGUU UCUCG...CUC-3'@LB@ AAAUG GGGGCA CA GGCAGUGGC@LB@ \|\|\|\|\| \|\|\|:\|\| \|\| :\|\|\|\|\|\|\|\|@LB@ UUUAC CCCUGU GU UCGUCACCG@LB@3'-UUU...GGUGU A UUCUUU -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -15.64 kcal/mol@LB@Hybridization Energy -29.0551 kcal/mol@LB@Unfolding Energy - mRNA 10.8938 kcal/mol@LB@Unfolding Energy - sRNA 2.51907 kcal/mol@LB@@LB@Position - mRNA 24 -- 52@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 1 -- 7@LB@
161	0.01187	0.2504	b0093	ftsQ	-14.15	0.043981	32 -- 43	18 -- 29	divisome assembly protein membrane anchored protein involved in growth of wall at septum	_____________31___________44\n_____________\|____________\|\n5'-GCU...GCAAA____________AUUGA...CCG-3'\n______________GGAUGGGGACGU\n______________::\|\|\|\|\|\|\|\|:\|\n______________UUUACCCCUGUA\n3'-UUU...GGUGU____________GUUUC...CCG-5'\n_____________\|____________\|\n_____________30___________17\n	37 -- 43	18 -- 24	-18.9266 kcal/mol	4.11166 kcal/mol	0.665542 kcal/mol	31 44@LB@ \| \|@LB@5'-GCU...GCAAA AUUGA...CCG-3'@LB@ GGAUGGGGACGU@LB@ ::\|\|\|\|\|\|\|\|:\|@LB@ UUUACCCCUGUA@LB@3'-UUU...GGUGU GUUUC...CCG-5'@LB@ \| \|@LB@ 30 17@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -18.9266 kcal/mol@LB@Unfolding Energy - mRNA 4.11166 kcal/mol@LB@Unfolding Energy - sRNA 0.665542 kcal/mol@LB@@LB@Position - mRNA 32 -- 43@LB@Position - sRNA 18 -- 29@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 18 -- 24@LB@
181	0.01309	0.2504	b2799	fucO	-15.36	0.022654	183 -- 205	1 -- 21	L-1 2-propanediol oxidoreductase	_____________182_____________________206\n_____________\|_______________________\|\n5'-CUU...GUAAA___A__________G_U_A____UAACA...CGC-3'\n______________GCA_CAAGGAGAAG_A_G_UGGC\n______________:\|\|_\|\|\|:\|\|:\|\|\|_\|_\|_\|\|\|\|\n______________UGU_GUUUCUUUUC_U_C_ACCG\n3'-UUU...ACCCC___A__________G________-5'\n_____________\|______________________\|\n_____________22_____________________1\n	190 -- 196	8 -- 14	-20.3858 kcal/mol	3.51699 kcal/mol	1.51103 kcal/mol	182 206@LB@ \| \|@LB@5'-CUU...GUAAA A G U A UAACA...CGC-3'@LB@ GCA CAAGGAGAAG A G UGGC@LB@ :\|\| \|\|\|:\|\|:\|\|\| \| \| \|\|\|\|@LB@ UGU GUUUCUUUUC U C ACCG@LB@3'-UUU...ACCCC A G -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -20.3858 kcal/mol@LB@Unfolding Energy - mRNA 3.51699 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 183 -- 205@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 8 -- 14@LB@
124	0.008958	0.2491	b0124	gcd	-13.85	0.051383	179 -- 193	48 -- 62	glucose dehydrogenase	_____________178_____________194\n_____________\|_______________\|\n5'-UAA...AUUAA_______________UCUCU...GGC-3'\n______________UGAAUUGAAAUGGUG\n______________\|\|\|\|::\|\|\|\|\|\|\|:\|\n______________ACUUGGCUUUACCGC\n3'-UUU...UUGGA_______________CCCAA...CCG-5'\n_____________\|_______________\|\n_____________63______________47\n	187 -- 193	48 -- 54	-20.4838 kcal/mol	2.67361 kcal/mol	3.96136 kcal/mol	178 194@LB@ \| \|@LB@5'-UAA...AUUAA UCUCU...GGC-3'@LB@ UGAAUUGAAAUGGUG@LB@ \|\|\|\|::\|\|\|\|\|\|\|:\|@LB@ ACUUGGCUUUACCGC@LB@3'-UUU...UUGGA CCCAA...CCG-5'@LB@ \| \|@LB@ 63 47@LB@@LB@@LB@Energy -13.85 kcal/mol@LB@Hybridization Energy -20.4838 kcal/mol@LB@Unfolding Energy - mRNA 2.67361 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 179 -- 193@LB@Position - sRNA 48 -- 62@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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