Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
164	0.01206	0.2504	b0882	clpA	-9.12	0.369525	95 -- 107	48 -- 60	ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease chaperone activity		_____________94____________108\n_____________\|_____________\|\n5'-UUU...GCAGA____A________AUGGU...CGC-3'\n______________AACC_AAGUGGCG\n______________\|\|\|\|_\|\|:\|\|\|\|\|\n______________UUGG_UUUACCGC\n3'-UUU...GGAAC____C________CCCAA...CCG-5'\n_____________\|_____________\|\n_____________61____________47\n	101 -- 107	48 -- 54	-16.8445 kcal/mol	3.76103 kcal/mol	3.96136 kcal/mol	94 108@LB@ \| \|@LB@5'-UUU...GCAGA A AUGGU...CGC-3'@LB@ AACC AAGUGGCG@LB@ \|\|\|\| \|\|:\|\|\|\|\|@LB@ UUGG UUUACCGC@LB@3'-UUU...GGAAC C CCCAA...CCG-5'@LB@ \| \|@LB@ 61 47@LB@@LB@@LB@Energy -9.12 kcal/mol@LB@Hybridization Energy -16.8445 kcal/mol@LB@Unfolding Energy - mRNA 3.76103 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 95 -- 107@LB@Position - sRNA 48 -- 60@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 48 -- 54@LB@
52	0.002562	0.1729	b0881	clpS	-16.21	0.013666	11 -- 25	5 -- 25	regulatory protein for ClpA substrate specificity		_____________10____________________26\n_____________\|_____________________\|\n5'-GCA...UGACA_____________________GUAUG...UCA-3'\n______________GGGGACA______AGGGGCAG\n______________\|\|\|\|\|\|\|______\|:::\|\|\|\|\n______________CCCCUGU______UUUUCGUC\n3'-UUU...UUUUA_______AGUUUC________ACCG-5'\n_____________\|_____________________\|\n_____________26____________________4\n	19 -- 25	5 -- 11	-21.27 kcal/mol	2.56205 kcal/mol	2.50017 kcal/mol	10 26@LB@ \| \|@LB@5'-GCA...UGACA GUAUG...UCA-3'@LB@ GGGGACA AGGGGCAG@LB@ \|\|\|\|\|\|\| \|:::\|\|\|\|@LB@ CCCCUGU UUUUCGUC@LB@3'-UUU...UUUUA AGUUUC ACCG-5'@LB@ \| \|@LB@ 26 4@LB@@LB@@LB@Energy -16.21 kcal/mol@LB@Hybridization Energy -21.27 kcal/mol@LB@Unfolding Energy - mRNA 2.56205 kcal/mol@LB@Unfolding Energy - sRNA 2.50017 kcal/mol@LB@@LB@Position - mRNA 11 -- 25@LB@Position - sRNA 5 -- 25@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 5 -- 11@LB@
111	0.007434	0.2348	b0103	coaE	-19.66	0.001155	224 -- 242	1 -- 25	dephospho-CoA kinase		_____________223________________________243\n_____________\|__________________________\|\n5'-UAU...UUAAC___A______________________AAGAG...AUA-3'\n______________GGG_GGCAUU_______GGCAGUGGC\n______________\|\|\|_\|:\|\|\|:_______:\|\|\|\|\|\|\|\|\n______________CCC_CUGUAG_______UCGUCACCG\n3'-UUU...UUUUA__________UUUCUUU_________-5'\n_____________\|_________________________\|\n_____________26________________________1\n	236 -- 242	1 -- 7	-28.3462 kcal/mol	6.16994 kcal/mol	2.51748 kcal/mol	223 243@LB@ \| \|@LB@5'-UAU...UUAAC A AAGAG...AUA-3'@LB@ GGG GGCAUU GGCAGUGGC@LB@ \|\|\| \|:\|\|\|: :\|\|\|\|\|\|\|\|@LB@ CCC CUGUAG UCGUCACCG@LB@3'-UUU...UUUUA UUUCUUU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -19.66 kcal/mol@LB@Hybridization Energy -28.3462 kcal/mol@LB@Unfolding Energy - mRNA 6.16994 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 224 -- 242@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 1 -- 7@LB@
132	0.00937	0.2491	b1120	cobB	-12.17	0.114836	175 -- 191	3 -- 18	deacetylase of acs and cheY chemotaxis regulator		_____________174_______________192\n_____________\|_________________\|\n5'-CGC...UUUUU_______U_________CCGGA...AUC-3'\n______________UCAGAGA_AAAGUGGUG\n______________\|\|\|:\|\|\|_\|\|\|\|::\|\|\|\n______________AGUUUCU_UUUCGUCAC\n3'-UUU...CCUGU_________________CG-5'\n_____________\|_________________\|\n_____________19________________2\n	185 -- 191	3 -- 9	-17.0979 kcal/mol	3.47557 kcal/mol	1.45388 kcal/mol	174 192@LB@ \| \|@LB@5'-CGC...UUUUU U CCGGA...AUC-3'@LB@ UCAGAGA AAAGUGGUG@LB@ \|\|\|:\|\|\| \|\|\|\|::\|\|\|@LB@ AGUUUCU UUUCGUCAC@LB@3'-UUU...CCUGU CG-5'@LB@ \| \|@LB@ 19 2@LB@@LB@@LB@Energy -12.17 kcal/mol@LB@Hybridization Energy -17.0979 kcal/mol@LB@Unfolding Energy - mRNA 3.47557 kcal/mol@LB@Unfolding Energy - sRNA 1.45388 kcal/mol@LB@@LB@Position - mRNA 175 -- 191@LB@Position - sRNA 3 -- 18@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 3 -- 9@LB@
40	0.001973	0.1648	b1823	cspC	-10.89	0.196769	199 -- 231	1 -- 29	stress protein member of the CspA-family		_____________198_______________________________232\n_____________\|_________________________________\|\n5'-UAU...UUUUC______CAA_G________UC____UA______UCAAC...UCU-3'\n______________AAAUGG___A_AUUAAAGG__AGGU__AGUGGU\n______________\|\|\|\|\|\|___\|_\|\|:\|\|\|\|:__\|:\|:__\|\|\|\|\|:\n______________UUUACC___U_UAGUUUCU__UUCG__UCACCG\n3'-UUU...GGUGU______CC__G________U_____________-5'\n_____________\|________________________________\|\n_____________30_______________________________1\n	210 -- 216	13 -- 19	-20.1954 kcal/mol	6.78842 kcal/mol	2.51907 kcal/mol	198 232@LB@ \| \|@LB@5'-UAU...UUUUC CAA G UC UA UCAAC...UCU-3'@LB@ AAAUGG A AUUAAAGG AGGU AGUGGU@LB@ \|\|\|\|\|\| \| \|\|:\|\|\|\|: \|:\|: \|\|\|\|\|:@LB@ UUUACC U UAGUUUCU UUCG UCACCG@LB@3'-UUU...GGUGU CC G U -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -10.89 kcal/mol@LB@Hybridization Energy -20.1954 kcal/mol@LB@Unfolding Energy - mRNA 6.78842 kcal/mol@LB@Unfolding Energy - sRNA 2.51907 kcal/mol@LB@@LB@Position - mRNA 199 -- 231@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 13 -- 19@LB@
119	0.008338	0.2459	b0123	cueO	-14.31	0.040489	47 -- 61	9 -- 24	multicopper oxidase (laccase)		_____________46_______________62\n_____________\|________________\|\n5'-CUG...UACCA________________UAAAC...CGU-3'\n______________GGGGC_UUAAAGAAGA\n______________\|\|\|:\|_\|:\|\|\|\|\|\|:\|\n______________CCCUG_AGUUUCUUUU\n3'-UUU...UUUAC_____U__________CGUCACCG-5'\n_____________\|________________\|\n_____________25_______________8\n	55 -- 61	9 -- 15	-17.0719 kcal/mol	2.10565 kcal/mol	0.659805 kcal/mol	46 62@LB@ \| \|@LB@5'-CUG...UACCA UAAAC...CGU-3'@LB@ GGGGC UUAAAGAAGA@LB@ \|\|\|:\| \|:\|\|\|\|\|\|:\|@LB@ CCCUG AGUUUCUUUU@LB@3'-UUU...UUUAC U CGUCACCG-5'@LB@ \| \|@LB@ 25 8@LB@@LB@@LB@Energy -14.31 kcal/mol@LB@Hybridization Energy -17.0719 kcal/mol@LB@Unfolding Energy - mRNA 2.10565 kcal/mol@LB@Unfolding Energy - sRNA 0.659805 kcal/mol@LB@@LB@Position - mRNA 47 -- 61@LB@Position - sRNA 9 -- 24@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 9 -- 15@LB@
172	0.01269	0.2504	b0571	cusR	-10.48	0.230388	14 -- 48	1 -- 33	response regulator in two-component regulatory system with CusS	b1969	_____________13___________________________________49\n_____________\|____________________________________\|\n5'-AGC...GGCCA__________CA__A___UAC__________UA___UCAUA...GGC-3'\n______________CACAAAAUGG__GA_GUU___AAGGAGA_CA__GGC\n______________\|\|\|\|\|\|\|\|\|\|__\|\|_:\|:___\|\|\|:\|:\|_\|\|__\|\|\|\n______________GUGUUUUACC__CU_UAG___UUCUUUU_GU__CCG\n3'-UUU...CCGAG__________C___G___U_________C__CA___-5'\n_____________\|___________________________________\|\n_____________34__________________________________1\n	35 -- 41	9 -- 15	-22.821 kcal/mol	9.26274 kcal/mol	3.07861 kcal/mol	13 49@LB@ \| \|@LB@5'-AGC...GGCCA CA A UAC UA UCAUA...GGC-3'@LB@ CACAAAAUGG GA GUU AAGGAGA CA GGC@LB@ \|\|\|\|\|\|\|\|\|\| \|\| :\|: \|\|\|:\|:\| \|\| \|\|\|@LB@ GUGUUUUACC CU UAG UUCUUUU GU CCG@LB@3'-UUU...CCGAG C G U C CA -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -22.821 kcal/mol@LB@Unfolding Energy - mRNA 9.26274 kcal/mol@LB@Unfolding Energy - sRNA 3.07861 kcal/mol@LB@@LB@Position - mRNA 14 -- 48@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 9 -- 15@LB@
129	0.009293	0.2491	b0428	cyoE	-12.51	0.098557	18 -- 26	1 -- 9	protoheme IX farnesyltransferase		_____________17________27\n_____________\|_________\|\n5'-GGA...GCAAU_________ACAGG...GCA-3'\n______________GGCAGUGGU\n______________:\|\|\|\|\|\|\|:\n______________UCGUCACCG\n3'-UUU...UCUUU_________-5'\n_____________\|________\|\n_____________10_______1\n	20 -- 26	1 -- 7	-15.8954 kcal/mol	2.13019 kcal/mol	1.2588 kcal/mol	17 27@LB@ \| \|@LB@5'-GGA...GCAAU ACAGG...GCA-3'@LB@ GGCAGUGGU@LB@ :\|\|\|\|\|\|\|:@LB@ UCGUCACCG@LB@3'-UUU...UCUUU -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -12.51 kcal/mol@LB@Hybridization Energy -15.8954 kcal/mol@LB@Unfolding Energy - mRNA 2.13019 kcal/mol@LB@Unfolding Energy - sRNA 1.2588 kcal/mol@LB@@LB@Position - mRNA 18 -- 26@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 1 -- 7@LB@
190	0.01401	0.2529	b2478	dapA	-10.08	0.266807	73 -- 98	6 -- 25	dihydrodipicolinate synthase		_____________72_________________________99\n_____________\|__________________________\|\n5'-GGC...UUUAA____UGA____UUCUU__________UAAAA...CCA-3'\n______________GGGG___GUUG_____AAGGAAAGCA\n______________\|\|\|\|___:\|::_____\|\|\|:\|\|\|\|\|\|\n______________CCCC___UAGU_____UUCUUUUCGU\n3'-UUU...UUUUA____UG____________________CACCG-5'\n_____________\|__________________________\|\n_____________26_________________________5\n	92 -- 98	6 -- 12	-17.2074 kcal/mol	6.41889 kcal/mol	0.70636 kcal/mol	72 99@LB@ \| \|@LB@5'-GGC...UUUAA UGA UUCUU UAAAA...CCA-3'@LB@ GGGG GUUG AAGGAAAGCA@LB@ \|\|\|\| :\|:: \|\|\|:\|\|\|\|\|\|@LB@ CCCC UAGU UUCUUUUCGU@LB@3'-UUU...UUUUA UG CACCG-5'@LB@ \| \|@LB@ 26 5@LB@@LB@@LB@Energy -10.08 kcal/mol@LB@Hybridization Energy -17.2074 kcal/mol@LB@Unfolding Energy - mRNA 6.41889 kcal/mol@LB@Unfolding Energy - sRNA 0.70636 kcal/mol@LB@@LB@Position - mRNA 73 -- 98@LB@Position - sRNA 6 -- 25@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 6 -- 12@LB@
2	5.107e-11	8.961e-08	b2314	dedD	-25.37	0.000001	176 -- 205	1 -- 30	membrane-anchored periplasmic protein involved in septation		_____________175_____________________________206\n_____________\|_______________________________\|\n5'-UUG...UCAUG______AUU______________________AAGUA...AUU-3'\n______________GAAGUG___GACG_CGAGGAGAAGCGGUGGC\n______________:\|\|:\|\|___\|\|\|:_\|:\|:\|\|:\|\|\|\|:\|\|\|\|\|\n______________UUUUAC___CUGU_GUUUCUUUUCGUCACCG\n3'-UUU...AGGUG______CC_____A_________________-5'\n_____________\|______________________________\|\n_____________31_____________________________1\n	199 -- 205	1 -- 7	-36.5756 kcal/mol	8.68219 kcal/mol	2.51911 kcal/mol	175 206@LB@ \| \|@LB@5'-UUG...UCAUG AUU AAGUA...AUU-3'@LB@ GAAGUG GACG CGAGGAGAAGCGGUGGC@LB@ :\|\|:\|\| \|\|\|: \|:\|:\|\|:\|\|\|\|:\|\|\|\|\|@LB@ UUUUAC CUGU GUUUCUUUUCGUCACCG@LB@3'-UUU...AGGUG CC A -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -25.37 kcal/mol@LB@Hybridization Energy -36.5756 kcal/mol@LB@Unfolding Energy - mRNA 8.68219 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 176 -- 205@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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