Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
12	0.0004023	0.1087	b3176	glmM	-16.74	0.009770	131 -- 153	1 -- 22	phosphoglucosamine mutase		_____________130_____________________154\n_____________\|_______________________\|\n5'-GUU...UUCAU_____________CC____AA__GAUGC...CGG-3'\n______________GACGUCAAAGAAA__GUAG__GC\n______________\|\|\|:\|\|\|\|\|\|\|\|\|__\|:\|\|__\|\|\n______________CUGUAGUUUCUUU__CGUC__CG\n3'-UUU...UACCC_____________U_____AC__-5'\n_____________\|______________________\|\n_____________23_____________________1\n	137 -- 143	10 -- 16	-21.6768 kcal/mol	3.39473 kcal/mol	1.54023 kcal/mol	130 154@LB@ \| \|@LB@5'-GUU...UUCAU CC AA GAUGC...CGG-3'@LB@ GACGUCAAAGAAA GUAG GC@LB@ \|\|\|:\|\|\|\|\|\|\|\|\| \|:\|\| \|\|@LB@ CUGUAGUUUCUUU CGUC CG@LB@3'-UUU...UACCC U AC -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -16.74 kcal/mol@LB@Hybridization Energy -21.6768 kcal/mol@LB@Unfolding Energy - mRNA 3.39473 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 131 -- 153@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 137 -- 143@LB@Position Seed - sRNA 10 -- 16@LB@
94	0.005808	0.2147	b0652	gltL	-13.33	0.066498	266 -- 292	1 -- 31	glutamate/aspartate ABC transporter ATPase		_____________265_____________________________293\n_____________\|_______________________________\|\n5'-CUC...UCAAC_______AAAA________C___________GGUUUGCG-3'\n______________CGAAGUG________AAGG_GAAGUGGUGGU\n______________\|:\|\|:\|\|________\|\|:\|_:\|\|\|::\|\|\|\|:\n______________GUUUUAC________UUUC_UUUCGUCACCG\n3'-UUU...GAGGU_______CCCUGUAG____U___________-5'\n_____________\|______________________________\|\n_____________32_____________________________1\n	286 -- 292	1 -- 7	-20.9442 kcal/mol	5.07032 kcal/mol	2.53929 kcal/mol	265 293@LB@ \| \|@LB@5'-CUC...UCAAC AAAA C GGUUUGCG-3'@LB@ CGAAGUG AAGG GAAGUGGUGGU@LB@ \|:\|\|:\|\| \|\|:\| :\|\|\|::\|\|\|\|:@LB@ GUUUUAC UUUC UUUCGUCACCG@LB@3'-UUU...GAGGU CCCUGUAG U -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -13.33 kcal/mol@LB@Hybridization Energy -20.9442 kcal/mol@LB@Unfolding Energy - mRNA 5.07032 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 266 -- 292@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 1 -- 7@LB@
133	0.009634	0.2504	b2551	glyA	-13.83	0.051752	272 -- 286	6 -- 21	serine hydroxymethyltransferase		_____________271______________287\n_____________\|________________\|\n5'-CAA...AAAGU________________CAUCG...UCG-3'\n______________ACGUC_AGGAAGAGCA\n______________\|\|:\|\|_\|:\|\|\|:\|\|\|\|\n______________UGUAG_UUCUUUUCGU\n3'-UUU...ACCCC_____U__________CACCG-5'\n_____________\|________________\|\n_____________22_______________5\n	280 -- 286	6 -- 12	-16.7043 kcal/mol	2.28484 kcal/mol	0.584589 kcal/mol	271 287@LB@ \| \|@LB@5'-CAA...AAAGU CAUCG...UCG-3'@LB@ ACGUC AGGAAGAGCA@LB@ \|\|:\|\| \|:\|\|\|:\|\|\|\|@LB@ UGUAG UUCUUUUCGU@LB@3'-UUU...ACCCC U CACCG-5'@LB@ \| \|@LB@ 22 5@LB@@LB@@LB@Energy -13.83 kcal/mol@LB@Hybridization Energy -16.7043 kcal/mol@LB@Unfolding Energy - mRNA 2.28484 kcal/mol@LB@Unfolding Energy - sRNA 0.584589 kcal/mol@LB@@LB@Position - mRNA 272 -- 286@LB@Position - sRNA 6 -- 21@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 6 -- 12@LB@
70	0.003861	0.1936	b3559	glyS	-15.28	0.023738	120 -- 130	1 -- 11	glycine tRNA synthetase beta subunit		_____________119_________131\n_____________\|___________\|\n5'-CGC...UGACC___________AGAAG...CGG-3'\n______________AAAGCAGUGGC\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________UUUCGUCACCG\n3'-UUU...UUUCU___________-5'\n_____________\|__________\|\n_____________12_________1\n	124 -- 130	1 -- 7	-20.0617 kcal/mol	3.50787 kcal/mol	1.27751 kcal/mol	119 131@LB@ \| \|@LB@5'-CGC...UGACC AGAAG...CGG-3'@LB@ AAAGCAGUGGC@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ UUUCGUCACCG@LB@3'-UUU...UUUCU -5'@LB@ \| \|@LB@ 12 1@LB@@LB@@LB@Energy -15.28 kcal/mol@LB@Hybridization Energy -20.0617 kcal/mol@LB@Unfolding Energy - mRNA 3.50787 kcal/mol@LB@Unfolding Energy - sRNA 1.27751 kcal/mol@LB@@LB@Position - mRNA 120 -- 130@LB@Position - sRNA 1 -- 11@LB@Position Seed - mRNA 124 -- 130@LB@Position Seed - sRNA 1 -- 7@LB@
138	0.0102	0.2504	b0830	gsiB	-12.88	0.082739	282 -- 298	1 -- 16	glutathione ABC transporter periplasmic binding protein		_____________281_______________299\n_____________\|_________________\|\n5'-GGC...CUGCC_____UGU_________GG-3'\n______________AAAGA___GGUGGUGGC\n______________\|\|\|\|\|___:\|::\|\|\|\|\|\n______________UUUCU___UCGUCACCG\n3'-UUU...UGUAG_____UU__________-5'\n_____________\|________________\|\n_____________17_______________1\n	292 -- 298	1 -- 7	-17.0557 kcal/mol	2.82856 kcal/mol	1.34555 kcal/mol	281 299@LB@ \| \|@LB@5'-GGC...CUGCC UGU GG-3'@LB@ AAAGA GGUGGUGGC@LB@ \|\|\|\|\| :\|::\|\|\|\|\|@LB@ UUUCU UCGUCACCG@LB@3'-UUU...UGUAG UU -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -17.0557 kcal/mol@LB@Unfolding Energy - mRNA 2.82856 kcal/mol@LB@Unfolding Energy - sRNA 1.34555 kcal/mol@LB@@LB@Position - mRNA 282 -- 298@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 1 -- 7@LB@
35	0.00168	0.1648	b2231	gyrA	-15.33	0.023033	230 -- 249	7 -- 25	DNA gyrase (type II topoisomerase) subunit A		_____________229__________________250\n_____________\|____________________\|\n5'-AGC...ACACC__UCA_______________UGAAG...CGC-3'\n______________GG___ACAUUGAGGAAGAGC\n______________\|\|___\|\|\|\|::\|:\|\|\|:\|\|\|\n______________CC___UGUAGUUUCUUUUCG\n3'-UUU...UUUUA__CC________________UCACCG-5'\n_____________\|____________________\|\n_____________26___________________6\n	243 -- 249	7 -- 13	-19.3394 kcal/mol	3.30418 kcal/mol	0.70626 kcal/mol	229 250@LB@ \| \|@LB@5'-AGC...ACACC UCA UGAAG...CGC-3'@LB@ GG ACAUUGAGGAAGAGC@LB@ \|\| \|\|\|\|::\|:\|\|\|:\|\|\|@LB@ CC UGUAGUUUCUUUUCG@LB@3'-UUU...UUUUA CC UCACCG-5'@LB@ \| \|@LB@ 26 6@LB@@LB@@LB@Energy -15.33 kcal/mol@LB@Hybridization Energy -19.3394 kcal/mol@LB@Unfolding Energy - mRNA 3.30418 kcal/mol@LB@Unfolding Energy - sRNA 0.70626 kcal/mol@LB@@LB@Position - mRNA 230 -- 249@LB@Position - sRNA 7 -- 25@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 7 -- 13@LB@
173	0.01269	0.2504	b4172	hfq	-12.44	0.101529	181 -- 205	1 -- 21	global sRNA chaperone; HF-I host factor for RNA phage Q beta replication		_____________180_______________________206\n_____________\|_________________________\|\n5'-GAA...AAUAA____A__________AG__AA____UAAGG...AAG-3'\n______________GCAU_UAAGGAAAAG__AG__UGGC\n______________:\|\|\|_:\|\|:\|\|\|\|\|\|__\|\|__\|\|\|\|\n______________UGUA_GUUUCUUUUC__UC__ACCG\n3'-UUU...ACCCC_______________G_________-5'\n_____________\|________________________\|\n_____________22_______________________1\n	189 -- 195	8 -- 14	-17.6301 kcal/mol	3.67752 kcal/mol	1.51103 kcal/mol	180 206@LB@ \| \|@LB@5'-GAA...AAUAA A AG AA UAAGG...AAG-3'@LB@ GCAU UAAGGAAAAG AG UGGC@LB@ :\|\|\| :\|\|:\|\|\|\|\|\| \|\| \|\|\|\|@LB@ UGUA GUUUCUUUUC UC ACCG@LB@3'-UUU...ACCCC G -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -12.44 kcal/mol@LB@Hybridization Energy -17.6301 kcal/mol@LB@Unfolding Energy - mRNA 3.67752 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 181 -- 205@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 8 -- 14@LB@
20	0.0007622	0.1243	b1507	hipA	-19.17	0.001718	1 -- 29	1 -- 31	inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase		______________1________________________________30\n______________\|________________________________\|\n___________5'-________CU___________C___________GAAAA...UAG-3'\n______________CAAAAUGG__GGACG_CAGAG__GAGC__UGGC\n______________\|\|\|\|\|\|\|\|__\|\|\|\|:_\|\|:\|\|__:\|\|\|__\|\|\|\|\n______________GUUUUACC__CCUGU_GUUUC__UUCG__ACCG\n3'-UUU...GAGGU_______________A_____UU____UC____-5'\n_____________\|________________________________\|\n_____________32_______________________________1\n	2 -- 8	24 -- 30	-30.0086 kcal/mol	8.29611 kcal/mol	2.53929 kcal/mol	1 30@LB@ \| \|@LB@ 5'- CU C GAAAA...UAG-3'@LB@ CAAAAUGG GGACG CAGAG GAGC UGGC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|: \|\|:\|\| :\|\|\| \|\|\|\|@LB@ GUUUUACC CCUGU GUUUC UUCG ACCG@LB@3'-UUU...GAGGU A UU UC -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -19.17 kcal/mol@LB@Hybridization Energy -30.0086 kcal/mol@LB@Unfolding Energy - mRNA 8.29611 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 1 -- 29@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 24 -- 30@LB@
19	0.000738	0.1243	b1508	hipB	-18.00	0.004180	266 -- 295	1 -- 32	antitoxin of HipAB toxin-antitoxin system	b1299 b1434	_____________265________________________________296\n_____________\|__________________________________\|\n5'-AUG...CGCCA_________CU___________C___________GAAAA-3'\n______________GCAAAAUGG__GGACG_CAGAG__GAGC__UGGC\n______________:\|\|\|\|\|\|\|\|__\|\|\|\|:_\|\|:\|\|__:\|\|\|__\|\|\|\|\n______________UGUUUUACC__CCUGU_GUUUC__UUCG__ACCG\n3'-UUU...CGAGG________________A_____UU____UC____-5'\n_____________\|_________________________________\|\n_____________33________________________________1\n	268 -- 274	24 -- 30	-30.0826 kcal/mol	9.54799 kcal/mol	2.53929 kcal/mol	265 296@LB@ \| \|@LB@5'-AUG...CGCCA CU C GAAAA-3'@LB@ GCAAAAUGG GGACG CAGAG GAGC UGGC@LB@ :\|\|\|\|\|\|\|\| \|\|\|\|: \|\|:\|\| :\|\|\| \|\|\|\|@LB@ UGUUUUACC CCUGU GUUUC UUCG ACCG@LB@3'-UUU...CGAGG A UU UC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -30.0826 kcal/mol@LB@Unfolding Energy - mRNA 9.54799 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 266 -- 295@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 24 -- 30@LB@
117	0.008014	0.2404	b3052	hldE	-12.77	0.087254	132 -- 161	1 -- 30	heptose 7-phosphate kinase and heptose 1-phosphate adenyltransferase		_____________131______________________________162\n_____________\|________________________________\|\n5'-GCG...UGGCA_______CUG___G_____AU___________AUUAU...CGC-3'\n______________GAAGUGG____GU_GAAGA__GAAGUA_UGGU\n______________:\|\|:\|\|\|____:\|_:\|\|\|\|__:\|\|\|:\|_\|\|\|:\n______________UUUUACC____UA_UUUCU__UUUCGU_ACCG\n3'-UUU...AGGUG_______CCUG__G_____________C____-5'\n_____________\|_______________________________\|\n_____________31______________________________1\n	132 -- 138	24 -- 30	-21.0542 kcal/mol	5.76621 kcal/mol	2.51911 kcal/mol	131 162@LB@ \| \|@LB@5'-GCG...UGGCA CUG G AU AUUAU...CGC-3'@LB@ GAAGUGG GU GAAGA GAAGUA UGGU@LB@ :\|\|:\|\|\| :\| :\|\|\|\| :\|\|\|:\| \|\|\|:@LB@ UUUUACC UA UUUCU UUUCGU ACCG@LB@3'-UUU...AGGUG CCUG G C -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -12.77 kcal/mol@LB@Hybridization Energy -21.0542 kcal/mol@LB@Unfolding Energy - mRNA 5.76621 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 132 -- 161@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 24 -- 30@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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