Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
159	0.01174	0.2504	b3886	yihY	-12.46	0.100688	17 -- 39	6 -- 26	BrkB family putative transporter inner membrane protein		_____________16______________________40\n_____________\|_______________________\|\n5'-UAU...AGAUC________CG____CCU______CGAAU...CAA-3'\n______________UGGGGAUG__CAAA___GAAGCA\n______________\|\|\|\|\|\|::__\|\|\|\|___:\|\|\|\|\|\n______________ACCCCUGU__GUUU___UUUCGU\n3'-UUU...GUUUU________A_____CU_______CACCG-5'\n_____________\|_______________________\|\n_____________27______________________5\n	18 -- 24	19 -- 25	-18.1196 kcal/mol	4.95346 kcal/mol	0.706421 kcal/mol	16 40@LB@ \| \|@LB@5'-UAU...AGAUC CG CCU CGAAU...CAA-3'@LB@ UGGGGAUG CAAA GAAGCA@LB@ \|\|\|\|\|\|:: \|\|\|\| :\|\|\|\|\|@LB@ ACCCCUGU GUUU UUUCGU@LB@3'-UUU...GUUUU A CU CACCG-5'@LB@ \| \|@LB@ 27 5@LB@@LB@@LB@Energy -12.46 kcal/mol@LB@Hybridization Energy -18.1196 kcal/mol@LB@Unfolding Energy - mRNA 4.95346 kcal/mol@LB@Unfolding Energy - sRNA 0.706421 kcal/mol@LB@@LB@Position - mRNA 17 -- 39@LB@Position - sRNA 6 -- 26@LB@Position Seed - mRNA 18 -- 24@LB@Position Seed - sRNA 19 -- 25@LB@
27	0.001238	0.1512	b4045	yjbJ	-9.99	0.275707	259 -- 267	19 -- 28	stress-induced protein UPF0337 family		_____________258________268\n_____________\|__________\|\n5'-CGU...AGAGC__________AACUG...AAG-3'\n______________AAUGGGG_CA\n______________\|\|\|\|\|\|\|_\|\|\n______________UUACCCC_GU\n3'-UUU...GUGUU_______U__AGUUU...CCG-5'\n_____________\|__________\|\n_____________29_________18\n	259 -- 265	22 -- 28	-11.8839 kcal/mol	1.24365 kcal/mol	0.649553 kcal/mol	258 268@LB@ \| \|@LB@5'-CGU...AGAGC AACUG...AAG-3'@LB@ AAUGGGG CA@LB@ \|\|\|\|\|\|\| \|\|@LB@ UUACCCC GU@LB@3'-UUU...GUGUU U AGUUU...CCG-5'@LB@ \| \|@LB@ 29 18@LB@@LB@@LB@Energy -9.99 kcal/mol@LB@Hybridization Energy -11.8839 kcal/mol@LB@Unfolding Energy - mRNA 1.24365 kcal/mol@LB@Unfolding Energy - sRNA 0.649553 kcal/mol@LB@@LB@Position - mRNA 259 -- 267@LB@Position - sRNA 19 -- 28@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 22 -- 28@LB@
33	0.001538	0.1635	b4141	yjeH	-13.95	0.048780	254 -- 275	1 -- 22	putative transporter		_____________253____________________276\n_____________\|______________________\|\n5'-CAG...CUAUC_______UUAUU_____C____GUGUU...CUG-3'\n______________GACGUCA_____AGGCA_UGGC\n______________\|\|\|:\|\|\|_____\|:\|\|\|_\|\|\|\|\n______________CUGUAGU_____UUCGU_ACCG\n3'-UUU...UACCC_______UUCUU_____C____-5'\n_____________\|_____________________\|\n_____________23____________________1\n	254 -- 260	16 -- 22	-22.2624 kcal/mol	6.77205 kcal/mol	1.54023 kcal/mol	253 276@LB@ \| \|@LB@5'-CAG...CUAUC UUAUU C GUGUU...CUG-3'@LB@ GACGUCA AGGCA UGGC@LB@ \|\|\|:\|\|\| \|:\|\|\| \|\|\|\|@LB@ CUGUAGU UUCGU ACCG@LB@3'-UUU...UACCC UUCUU C -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -13.95 kcal/mol@LB@Hybridization Energy -22.2624 kcal/mol@LB@Unfolding Energy - mRNA 6.77205 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 254 -- 275@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 16 -- 22@LB@
24	0.0009771	0.1361	b4295	yjhU	-16.20	0.013710	198 -- 226	5 -- 30	putative DNA-binding transcriptional regulator; KpLE2 phage-like element	b1512	_____________197___________________________227\n_____________\|_____________________________\|\n5'-GUA...AUACA_______AUA__G______C___C_____CCUGU...UUG-3'\n______________AAAGUGG___GA_AUCAAA_AAA_AGCAG\n______________\|\|\|:\|\|\|___\|\|_\|\|\|\|\|\|_\|\|\|_\|\|\|\|\|\n______________UUUUACC___CU_UAGUUU_UUU_UCGUC\n3'-UUU...AGGUG_______C____G______C_________ACCG-5'\n_____________\|_____________________________\|\n_____________31____________________________4\n	198 -- 204	24 -- 30	-21.4023 kcal/mol	2.69702 kcal/mol	2.50278 kcal/mol	197 227@LB@ \| \|@LB@5'-GUA...AUACA AUA G C C CCUGU...UUG-3'@LB@ AAAGUGG GA AUCAAA AAA AGCAG@LB@ \|\|\|:\|\|\| \|\| \|\|\|\|\|\| \|\|\| \|\|\|\|\|@LB@ UUUUACC CU UAGUUU UUU UCGUC@LB@3'-UUU...AGGUG C G C ACCG-5'@LB@ \| \|@LB@ 31 4@LB@@LB@@LB@Energy -16.20 kcal/mol@LB@Hybridization Energy -21.4023 kcal/mol@LB@Unfolding Energy - mRNA 2.69702 kcal/mol@LB@Unfolding Energy - sRNA 2.50278 kcal/mol@LB@@LB@Position - mRNA 198 -- 226@LB@Position - sRNA 5 -- 30@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 24 -- 30@LB@
65	0.00343	0.1851	b4363	yjjB	-10.89	0.196296	181 -- 193	20 -- 32	DUF3815 family inner membrane protein		_____________180___________194\n_____________\|_____________\|\n5'-ACU...CACUG____UU_______UACGC...GGG-3'\n______________ACGA__UGGGGGC\n______________\|\|:\|__\|\|\|\|\|:\|\n______________UGUU__ACCCCUG\n3'-UUU...CGAGG____UU_______UAGUU...CCG-5'\n_____________\|_____________\|\n_____________33____________19\n	187 -- 193	20 -- 26	-17.0561 kcal/mol	4.01516 kcal/mol	2.14699 kcal/mol	180 194@LB@ \| \|@LB@5'-ACU...CACUG UU UACGC...GGG-3'@LB@ ACGA UGGGGGC@LB@ \|\|:\| \|\|\|\|\|:\|@LB@ UGUU ACCCCUG@LB@3'-UUU...CGAGG UU UAGUU...CCG-5'@LB@ \| \|@LB@ 33 19@LB@@LB@@LB@Energy -10.89 kcal/mol@LB@Hybridization Energy -17.0561 kcal/mol@LB@Unfolding Energy - mRNA 4.01516 kcal/mol@LB@Unfolding Energy - sRNA 2.14699 kcal/mol@LB@@LB@Position - mRNA 181 -- 193@LB@Position - sRNA 20 -- 32@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 20 -- 26@LB@
108	0.007234	0.2348	b1725	yniA	-14.77	0.031588	182 -- 211	1 -- 32	fructosamine kinase family protein		_____________181_________________________________212\n_____________\|___________________________________\|\n5'-GAG...GGCAG____C_____AGA_______UGU____________AAUCA...GGC-3'\n______________ACAA_AUGGG___GACAUCA______GGCA__GGC\n______________\|\|\|\|_\|\|\|\|\|___\|\|\|\|\|\|\|______:\|\|\|__\|\|\|\n______________UGUU_UACCC___CUGUAGU______UCGU__CCG\n3'-UUU...CGAGG____U_______________UUCUUU____CA___-5'\n_____________\|__________________________________\|\n_____________33_________________________________1\n	195 -- 201	16 -- 22	-26.2676 kcal/mol	8.96188 kcal/mol	2.53929 kcal/mol	181 212@LB@ \| \|@LB@5'-GAG...GGCAG C AGA UGU AAUCA...GGC-3'@LB@ ACAA AUGGG GACAUCA GGCA GGC@LB@ \|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\| :\|\|\| \|\|\|@LB@ UGUU UACCC CUGUAGU UCGU CCG@LB@3'-UUU...CGAGG U UUCUUU CA -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -14.77 kcal/mol@LB@Hybridization Energy -26.2676 kcal/mol@LB@Unfolding Energy - mRNA 8.96188 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 182 -- 211@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 16 -- 22@LB@
7	0.0001218	0.05555	b1811	yoaH	-21.30	0.000256	259 -- 293	1 -- 35	UPF0181 family protein		_____________258__________________________________294\n_____________\|____________________________________\|\n5'-ACG...GCGGA____G___C__AU____________U______C___CAGGCAA-3'\n______________UCCA_GAA_UG__GGCA_CAAGGGA_GAGCAG_GGC\n______________\|\|\|\|_:\|\|_\|\|__\|:\|\|_\|\|\|:\|:\|_:\|\|\|\|\|_\|\|\|\n______________AGGU_UUU_AC__CUGU_GUUUCUU_UUCGUC_CCG\n3'-UUU...ACCCG____G___U__CC____A______________A___-5'\n_____________\|___________________________________\|\n_____________36__________________________________1\n	276 -- 282	11 -- 17	-33.9537 kcal/mol	7.61738 kcal/mol	5.03645 kcal/mol	258 294@LB@ \| \|@LB@5'-ACG...GCGGA G C AU U C CAGGCAA-3'@LB@ UCCA GAA UG GGCA CAAGGGA GAGCAG GGC@LB@ \|\|\|\| :\|\| \|\| \|:\|\| \|\|\|:\|:\| :\|\|\|\|\| \|\|\|@LB@ AGGU UUU AC CUGU GUUUCUU UUCGUC CCG@LB@3'-UUU...ACCCG G U CC A A -5'@LB@ \| \|@LB@ 36 1@LB@@LB@@LB@Energy -21.30 kcal/mol@LB@Hybridization Energy -33.9537 kcal/mol@LB@Unfolding Energy - mRNA 7.61738 kcal/mol@LB@Unfolding Energy - sRNA 5.03645 kcal/mol@LB@@LB@Position - mRNA 259 -- 293@LB@Position - sRNA 1 -- 35@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 11 -- 17@LB@
199	0.01447	0.2552	b2790	yqcA	-9.70	0.305430	160 -- 205	1 -- 31	short-chain flavodoxin FMN-binding		_____________159_____________________________________________206\n_____________\|_______________________________________________\|\n5'-CUA...CGGGU________A__G__AAGUUCCUAAUC________AAAC_________GGAAA...CAA-3'\n______________CAGGAUGG_GA_AU____________CAGGGAGA____GGUA_UGGC\n______________\|\|::\|\|\|\|_\|\|_\|\|____________\|\|::\|\|:\|____:\|:\|_\|\|\|\|\n______________GUUUUACC_CU_UA____________GUUUCUUU____UCGU_ACCG\n3'-UUU...GAGGU________C__G______________________________C____-5'\n_____________\|______________________________________________\|\n_____________32_____________________________________________1\n	187 -- 193	10 -- 16	-23.6635 kcal/mol	11.4257 kcal/mol	2.53929 kcal/mol	159 206@LB@ \| \|@LB@5'-CUA...CGGGU A G AAGUUCCUAAUC AAAC GGAAA...CAA-3'@LB@ CAGGAUGG GA AU CAGGGAGA GGUA UGGC@LB@ \|\|::\|\|\|\| \|\| \|\| \|\|::\|\|:\| :\|:\| \|\|\|\|@LB@ GUUUUACC CU UA GUUUCUUU UCGU ACCG@LB@3'-UUU...GAGGU C G C -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -23.6635 kcal/mol@LB@Unfolding Energy - mRNA 11.4257 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 160 -- 205@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 10 -- 16@LB@
89	0.005593	0.2147	b3398	yrfF	-14.72	0.032340	186 -- 206	1 -- 31	putative RcsCDB-response attenuator inner membrane protein		_____________185______________________________207\n_____________\|________________________________\|\n5'-UUA...CUAAG_____________________________A__ACCAU...GGA-3'\n______________UAAGAUGGGGA__________AAGCA_UG_GC\n______________:\|\|:\|\|\|\|\|\|\|__________\|\|\|\|\|_\|\|_\|\|\n______________GUUUUACCCCU__________UUCGU_AC_CG\n3'-UUU...GAGGU___________GUAGUUUCUU_____C_____-5'\n_____________\|_______________________________\|\n_____________32______________________________1\n	190 -- 196	21 -- 27	-21.8606 kcal/mol	4.59782 kcal/mol	2.53929 kcal/mol	185 207@LB@ \| \|@LB@5'-UUA...CUAAG A ACCAU...GGA-3'@LB@ UAAGAUGGGGA AAGCA UG GC@LB@ :\|\|:\|\|\|\|\|\|\| \|\|\|\|\| \|\| \|\|@LB@ GUUUUACCCCU UUCGU AC CG@LB@3'-UUU...GAGGU GUAGUUUCUU C -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -14.72 kcal/mol@LB@Hybridization Energy -21.8606 kcal/mol@LB@Unfolding Energy - mRNA 4.59782 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 186 -- 206@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 21 -- 27@LB@
166	0.01216	0.2504	b4485	ytfR	-12.60	0.094393	18 -- 41	9 -- 34	putative sugar ABC transporter ATPase		_____________17_________________________42\n_____________\|__________________________\|\n5'-CCU...UGAUA________GAA_______________AGAAA...GCC-3'\n______________CCGCAAAA____GA_AUUAGAGAAGA\n______________\|\|:\|\|\|\|\|____\|\|_\|\|:\|:\|\|\|\|:\|\n______________GGUGUUUU____CU_UAGUUUCUUUU\n3'-UUU...CCCGA________ACCC__G___________CGUCACCG-5'\n_____________\|__________________________\|\n_____________35_________________________8\n	35 -- 41	9 -- 15	-17.8797 kcal/mol	1.35017 kcal/mol	3.92941 kcal/mol	17 42@LB@ \| \|@LB@5'-CCU...UGAUA GAA AGAAA...GCC-3'@LB@ CCGCAAAA GA AUUAGAGAAGA@LB@ \|\|:\|\|\|\|\| \|\| \|\|:\|:\|\|\|\|:\|@LB@ GGUGUUUU CU UAGUUUCUUUU@LB@3'-UUU...CCCGA ACCC G CGUCACCG-5'@LB@ \| \|@LB@ 35 8@LB@@LB@@LB@Energy -12.60 kcal/mol@LB@Hybridization Energy -17.8797 kcal/mol@LB@Unfolding Energy - mRNA 1.35017 kcal/mol@LB@Unfolding Energy - sRNA 3.92941 kcal/mol@LB@@LB@Position - mRNA 18 -- 41@LB@Position - sRNA 9 -- 34@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 9 -- 15@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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