Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
95	0.01047	0.3575	b4232	fbp	-15.00	0.027086	59 -- 98	140 -- 173	fructose-1 6-bisphosphatase I	_____________58_______________________________________________99\n_____________\|________________________________________________\|\n5'-AAA...CUGCC_____UU____________U_________G_GAAAC__A_________AAUCC...CCA-3'\n______________GCUCA__UUACGCAU_AUG_CGGGCAAGU_A_____GC_UUUCAGGAU\n______________:\|\|\|\|__\|:\|\|:\|\|\|_\|\|\|_\|:\|\|\|\|\|\|\|_\|_____:\|_\|\|\|\|\|\|:\|\|\n______________UGAGU__AGUGUGUA_UAC_GUCCGUUCA_U_____UG_AAAGUCUUA\n3'-UUU...GGUUA_____C_________C_____________G__________________ACGCC...UAG-5'\n_____________\|________________________________________________\|\n_____________174______________________________________________139\n	92 -- 98	140 -- 146	-39.7088 kcal/mol	14.0477 kcal/mol	8.83532 kcal/mol	58 99@LB@ \| \|@LB@5'-AAA...CUGCC UU U G GAAAC A AAUCC...CCA-3'@LB@ GCUCA UUACGCAU AUG CGGGCAAGU A GC UUUCAGGAU@LB@ :\|\|\|\| \|:\|\|:\|\|\| \|\|\| \|:\|\|\|\|\|\|\| \| :\| \|\|\|\|\|\|:\|\|@LB@ UGAGU AGUGUGUA UAC GUCCGUUCA U UG AAAGUCUUA@LB@3'-UUU...GGUUA C C G ACGCC...UAG-5'@LB@ \| \|@LB@ 174 139@LB@@LB@@LB@Energy -15.00 kcal/mol@LB@Hybridization Energy -39.7088 kcal/mol@LB@Unfolding Energy - mRNA 14.0477 kcal/mol@LB@Unfolding Energy - sRNA 8.83532 kcal/mol@LB@@LB@Position - mRNA 59 -- 98@LB@Position - sRNA 140 -- 173@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 140 -- 146@LB@
35	0.002845	0.2736	b3887	dtd	-14.98	0.027287	55 -- 74	191 -- 211	D-tyr-tRNA(Tyr) deacylase	_____________54____________________75\n_____________\|_____________________\|\n5'-GGA...UCAUU______G______________CCCCA...UCG-3'\n______________UAUGGU_UGCUGGCGG_UGAU\n______________\|\|\|::\|_\|\|\|\|\|\|\|\|\|_\|\|\|\|\n______________AUAUUA_ACGACCGCC_ACUA\n3'-UUU...CGUCC______G_________C____AAAUG...UAG-5'\n_____________\|_____________________\|\n_____________212___________________190\n	64 -- 70	196 -- 202	-27.702 kcal/mol	6.2401 kcal/mol	11.5765 kcal/mol	54 75@LB@ \| \|@LB@5'-GGA...UCAUU G CCCCA...UCG-3'@LB@ UAUGGU UGCUGGCGG UGAU@LB@ \|\|\|::\| \|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ AUAUUA ACGACCGCC ACUA@LB@3'-UUU...CGUCC G C AAAUG...UAG-5'@LB@ \| \|@LB@ 212 190@LB@@LB@@LB@Energy -14.98 kcal/mol@LB@Hybridization Energy -27.702 kcal/mol@LB@Unfolding Energy - mRNA 6.2401 kcal/mol@LB@Unfolding Energy - sRNA 11.5765 kcal/mol@LB@@LB@Position - mRNA 55 -- 74@LB@Position - sRNA 191 -- 211@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 196 -- 202@LB@
125	0.01377	0.3575	b2827	thyA	-14.98	0.027366	100 -- 109	16 -- 25	thymidylate synthetase	_____________99_____________________110\n_____________\|______________________\|\n5'-CAG...AAACA________A_____________AAAAG...AGA-3'\n______________GUAUUUAG__AC___UGAUGCA\n______________:\|\|:\|:\|\|__\|\|___\|\|\|\|\|\|\|\n______________UAUGAGUC__UG___ACUACGU\n3'-UUU...GUGGU________AG__UGU_______CCGUU...UAG-5'\n_____________\|______________________\|\n_____________26_____________________15\n	220 -- 226	160 -- 166	-15.3976 kcal/mol	3.04146 kcal/mol	4.80633 kcal/mol	99 110@LB@ \| \|@LB@5'-CAG...AAACA A AAAAG...AGA-3'@LB@ GUAUUUAG AC UGAUGCA@LB@ :\|\|:\|:\|\| \|\| \|\|\|\|\|\|\|@LB@ UAUGAGUC UG ACUACGU@LB@3'-UUU...GUGGU AG UGU CCGUU...UAG-5'@LB@ \| \|@LB@ 26 15@LB@@LB@@LB@Energy -14.98 kcal/mol@LB@Hybridization Energy -15.3976 kcal/mol@LB@Unfolding Energy - mRNA 3.04146 kcal/mol@LB@Unfolding Energy - sRNA 4.80633 kcal/mol@LB@@LB@Position - mRNA 100 -- 109@LB@Position - sRNA 16 -- 25@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 160 -- 166@LB@
54	0.005402	0.3247	b3054	ygiF	-14.97	0.027422	5 -- 80	111 -- 177	inorganic triphosphatase	_____________4_______________________________________________________________________________81\n_____________\|_______________________________________________________________________________\|\n_______5'-GGCA_____GU___UAC_____AA___U______AUCAA_______CCUCUG___CA____U_G_______U___AA______CGCCC...CCG-3'\n______________UCAGU__CGC_____GCA__GCA__UCGAC_____UCGGAAU______UGU__GGUC_U_CCACUGC_UCA__CUUUUA\n______________\|\|\|\|\|__\|:\|_____\|\|\|__\|\|\|__\|\|:\|\|_____\|\|:\|\|\|\|______\|\|:__\|\|\|\|_\|_\|\|\|\|\|\|\|_\|\|\|__\|:\|\|:\|\n______________AGUCA__GUG_____CGU__CGU__AGUUG_____AGUCUUA______ACG__CCAG_A_GGUGACG_AGU__GGAAGU\n3'-UUU...UUAUG_____GU___UACUA___C____UC_____AA_________________________U_G_______U___CA______UCGUA...UAG-5'\n_____________\|_______________________________________________________________________________\|\n_____________178_____________________________________________________________________________110\n	62 -- 68	123 -- 129	-50.4846 kcal/mol	15.6316 kcal/mol	23.3828 kcal/mol	4 81@LB@ \| \|@LB@ 5'-GGCA GU UAC AA U AUCAA CCUCUG CA U G U AA CGCCC...CCG-3'@LB@ UCAGU CGC GCA GCA UCGAC UCGGAAU UGU GGUC U CCACUGC UCA CUUUUA@LB@ \|\|\|\|\| \|:\| \|\|\| \|\|\| \|\|:\|\| \|\|:\|\|\|\| \|\|: \|\|\|\| \| \|\|\|\|\|\|\| \|\|\| \|:\|\|:\|@LB@ AGUCA GUG CGU CGU AGUUG AGUCUUA ACG CCAG A GGUGACG AGU GGAAGU@LB@3'-UUU...UUAUG GU UACUA C UC AA U G U CA UCGUA...UAG-5'@LB@ \| \|@LB@ 178 110@LB@@LB@@LB@Energy -14.97 kcal/mol@LB@Hybridization Energy -50.4846 kcal/mol@LB@Unfolding Energy - mRNA 15.6316 kcal/mol@LB@Unfolding Energy - sRNA 23.3828 kcal/mol@LB@@LB@Position - mRNA 5 -- 80@LB@Position - sRNA 111 -- 177@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 123 -- 129@LB@
145	0.01541	0.3575	b1191	cvrA	-14.91	0.028165	61 -- 91	128 -- 160	putative cation/proton antiporter	_____________60___________________________________92\n_____________\|____________________________________\|\n5'-UGA...CGGGA_____CUC_______GA___________________GUUCU...CUA-3'\n______________AGGCA___AGUUGAC__UUA_____GCGGUCAUCCU\n______________\|\|\|\|\|___\|\|\|::\|\|__\|:\|_____\|\|\|\|\|\|\|\|\|\|:\n______________UCCGU___UCAGUUG__AGU_____CGCCAGUAGGG\n3'-UUU...CUACG_______________AA___CUUAA___________UGACG...UAG-5'\n_____________\|____________________________________\|\n_____________161__________________________________127\n	85 -- 91	128 -- 134	-32.547 kcal/mol	9.03914 kcal/mol	12.2557 kcal/mol	60 92@LB@ \| \|@LB@5'-UGA...CGGGA CUC GA GUUCU...CUA-3'@LB@ AGGCA AGUUGAC UUA GCGGUCAUCCU@LB@ \|\|\|\|\| \|\|\|::\|\| \|:\| \|\|\|\|\|\|\|\|\|\|:@LB@ UCCGU UCAGUUG AGU CGCCAGUAGGG@LB@3'-UUU...CUACG AA CUUAA UGACG...UAG-5'@LB@ \| \|@LB@ 161 127@LB@@LB@@LB@Energy -14.91 kcal/mol@LB@Hybridization Energy -32.547 kcal/mol@LB@Unfolding Energy - mRNA 9.03914 kcal/mol@LB@Unfolding Energy - sRNA 12.2557 kcal/mol@LB@@LB@Position - mRNA 61 -- 91@LB@Position - sRNA 128 -- 160@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 128 -- 134@LB@
42	0.003683	0.2912	b2221	atoD	-14.87	0.028783	141 -- 155	6 -- 19	acetyl-CoA:acetoacetyl-CoA transferase alpha subunit	_____________140_____________156\n_____________\|_______________\|\n5'-UAU...AUUCU_____U_________AUUGC...CCC-3'\n______________GGCAC_CCCCUUGCU\n______________\|\|\|\|\|_\|\|\|\|\|\|\|\|\|\n______________CCGUG_GGGGAACGA\n3'-UUU...GUACC_______________AGUAG-5'\n_____________\|_______________\|\n_____________20______________5\n	149 -- 155	6 -- 12	-27.0081 kcal/mol	4.79018 kcal/mol	8.30673 kcal/mol	140 156@LB@ \| \|@LB@5'-UAU...AUUCU U AUUGC...CCC-3'@LB@ GGCAC CCCCUUGCU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CCGUG GGGGAACGA@LB@3'-UUU...GUACC AGUAG-5'@LB@ \| \|@LB@ 20 5@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -27.0081 kcal/mol@LB@Unfolding Energy - mRNA 4.79018 kcal/mol@LB@Unfolding Energy - sRNA 8.30673 kcal/mol@LB@@LB@Position - mRNA 141 -- 155@LB@Position - sRNA 6 -- 19@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 6 -- 12@LB@
150	0.01679	0.3749	b3550	yiaC	-14.78	0.030008	125 -- 180	123 -- 174	GNAT family putative N-acetyltransferase	_____________124_______________________________________________181\n_____________\|_________________________________________________\|\n5'-AAU...UCUGU_____CU_______G_C________U___G___GA______________GGCUG...GGC-3'\n______________UACUC__UUAUGCA_G_AUGUGGGC_GGU_AAU__UCA____UGUGGUU\n______________\|\|\|\|\|__\|:\|::\|\|_\|_\|\|\|::\|\|\|_:\|\|_\|\|:__\|\|\|____\|\|:\|\|\|:\n______________AUGAG__AGUGUGU_C_UACGUCCG_UCA_UUG__AGU____ACGCCAG\n3'-UUU...UGGUU_____UC_______A__________U___G___AA___CUUA_______UAGGG...UAG-5'\n_____________\|_________________________________________________\|\n_____________175_______________________________________________122\n	134 -- 140	167 -- 173	-39.4647 kcal/mol	14.8163 kcal/mol	12.8033 kcal/mol	124 181@LB@ \| \|@LB@5'-AAU...UCUGU CU G C U G GA GGCUG...GGC-3'@LB@ UACUC UUAUGCA G AUGUGGGC GGU AAU UCA UGUGGUU@LB@ \|\|\|\|\| \|:\|::\|\| \| \|\|\|::\|\|\| :\|\| \|\|: \|\|\| \|\|:\|\|\|:@LB@ AUGAG AGUGUGU C UACGUCCG UCA UUG AGU ACGCCAG@LB@3'-UUU...UGGUU UC A U G AA CUUA UAGGG...UAG-5'@LB@ \| \|@LB@ 175 122@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -39.4647 kcal/mol@LB@Unfolding Energy - mRNA 14.8163 kcal/mol@LB@Unfolding Energy - sRNA 12.8033 kcal/mol@LB@@LB@Position - mRNA 125 -- 180@LB@Position - sRNA 123 -- 174@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 167 -- 173@LB@
193	0.0225	0.3833	b3751	rbsB	-14.76	0.030308	38 -- 56	74 -- 93	D-ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor	_____________37________________________________________________57\n_____________\|_________________________________________________\|\n___5'-GCGGUACG_____GG______GAAAA_____CA___U____________________UUAAU...UCU-3'\n______________AGUCU__CGGGCG_____GGUCG__UUG_UGGGACGUU_GAUCGGCGCA\n______________\|\|\|\|:__\|::\|\|:_____::\|\|\|__\|\|\|_\|\|\|\|\|\|\|:\|_:\|:\|::\|\|\|\|\n______________UCAGG__GUUCGU_____UUAGC__AAC_ACCCUGCGA_UUGGUUGCGU\n3'-UUU...CGUAG_____AA______ACCAA____________________A__________UGGUC...UAG-5'\n_____________\|_________________________________________________\|\n_____________94________________________________________________73\n	40 -- 46	85 -- 91	-47.7546 kcal/mol	17.619 kcal/mol	19.1533 kcal/mol	37 57@LB@ \| \|@LB@ 5'-GCGGUACG GG GAAAA CA U UUAAU...UCU-3'@LB@ AGUCU CGGGCG GGUCG UUG UGGGACGUU GAUCGGCGCA@LB@ \|\|\|\|: \|::\|\|: ::\|\|\| \|\|\| \|\|\|\|\|\|\|:\| :\|:\|::\|\|\|\|@LB@ UCAGG GUUCGU UUAGC AAC ACCCUGCGA UUGGUUGCGU@LB@3'-UUU...CGUAG AA ACCAA A UGGUC...UAG-5'@LB@ \| \|@LB@ 94 73@LB@@LB@@LB@Energy -14.76 kcal/mol@LB@Hybridization Energy -47.7546 kcal/mol@LB@Unfolding Energy - mRNA 17.619 kcal/mol@LB@Unfolding Energy - sRNA 19.1533 kcal/mol@LB@@LB@Position - mRNA 38 -- 56@LB@Position - sRNA 74 -- 93@LB@Position Seed - mRNA 40 -- 46@LB@Position Seed - sRNA 85 -- 91@LB@
7	0.0005448	0.1842	b1912	pgsA	-14.68	0.031521	247 -- 274	111 -- 134	phosphatidylglycerophosphate synthetase	_____________246__________________________275\n_____________\|____________________________\|\n5'-GUC...CAUCC_________UCUU_______________UCUGC...UUG-3'\n______________UUAUCCCAU____UGUAUUGGUCUUUUA\n______________\|:\|\|\|\|\|\|:____\|\|:\|\|::\|\|\|:\|\|:\|\n______________AGUAGGGUG____ACGUAGUCAGGAAGU\n3'-UUU...ACGCC____________________________UCGUA...UAG-5'\n_____________\|____________________________\|\n_____________135__________________________110\n	268 -- 274	111 -- 117	-27.9222 kcal/mol	1.79949 kcal/mol	14.1586 kcal/mol	246 275@LB@ \| \|@LB@5'-GUC...CAUCC UCUU UCUGC...UUG-3'@LB@ UUAUCCCAU UGUAUUGGUCUUUUA@LB@ \|:\|\|\|\|\|\|: \|\|:\|\|::\|\|\|:\|\|:\|@LB@ AGUAGGGUG ACGUAGUCAGGAAGU@LB@3'-UUU...ACGCC UCGUA...UAG-5'@LB@ \| \|@LB@ 135 110@LB@@LB@@LB@Energy -14.68 kcal/mol@LB@Hybridization Energy -27.9222 kcal/mol@LB@Unfolding Energy - mRNA 1.79949 kcal/mol@LB@Unfolding Energy - sRNA 14.1586 kcal/mol@LB@@LB@Position - mRNA 247 -- 274@LB@Position - sRNA 111 -- 134@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 111 -- 117@LB@
144	0.01541	0.3575	b4555	yicS	-14.57	0.033136	213 -- 290	115 -- 187	putative periplasmic protein	_____________212_________________________________________________________________291\n_____________\|___________________________________________________________________\|\n5'-AUU...CAACG______C______UUU____UUUUUUC__U_____C______UCGUAUACGCCG_____C_______CUCAC...AGA-3'\n______________ACACUA_UGCUUA___UCAC_______GC___AUG_CAGGCA____________AGUCG_CUUUUAG\n______________\|\|\|\|:\|_\|:\|\|:\|___\|\|\|\|_______:\|___\|\|\|_\|\|\|\|\|\|____________\|\|\|\|:_\|\|\|\|:\|\|\n______________UGUGGU_AUGAGU___AGUG_______UG___UAC_GUCCGU____________UCAGU_GAAAGUC\n3'-UUU...UAAAA______U______C_______________UAC___________________________U_______UUAAC...UAG-5'\n_____________\|___________________________________________________________________\|\n_____________188_________________________________________________________________114\n	271 -- 277	143 -- 149	-34.4495 kcal/mol	11.6435 kcal/mol	13.2932 kcal/mol	212 291@LB@ \| \|@LB@5'-AUU...CAACG C UUU UUUUUUC U C UCGUAUACGCCG C CUCAC...AGA-3'@LB@ ACACUA UGCUUA UCAC GC AUG CAGGCA AGUCG CUUUUAG@LB@ \|\|\|\|:\| \|:\|\|:\| \|\|\|\| :\| \|\|\| \|\|\|\|\|\| \|\|\|\|: \|\|\|\|:\|\|@LB@ UGUGGU AUGAGU AGUG UG UAC GUCCGU UCAGU GAAAGUC@LB@3'-UUU...UAAAA U C UAC U UUAAC...UAG-5'@LB@ \| \|@LB@ 188 114@LB@@LB@@LB@Energy -14.57 kcal/mol@LB@Hybridization Energy -34.4495 kcal/mol@LB@Unfolding Energy - mRNA 11.6435 kcal/mol@LB@Unfolding Energy - sRNA 13.2932 kcal/mol@LB@@LB@Position - mRNA 213 -- 290@LB@Position - sRNA 115 -- 187@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 143 -- 149@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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