Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
155	0.02668	0.5559	b1625	cnu	-8.12	0.218326	240 -- 246	81 -- 87	nucleoid-associated oriC-binding protein; H-NS and StpA stabilizing factor	_____________239_____247\n_____________\|_______\|\n5'-UUU...AAAUC_______UCGAA...AUC-3'\n______________AGUUCAC\n______________\|\|\|\|\|\|\|\n______________UCAAGUG\n3'-CGC...CGUUU_______CAACC...AAG-5'\n_____________\|_______\|\n_____________88______80\n	240 -- 246	81 -- 87	-9.1905 kcal/mol	0.576286 kcal/mol	0.494577 kcal/mol	239 247@LB@ \| \|@LB@5'-UUU...AAAUC UCGAA...AUC-3'@LB@ AGUUCAC@LB@ \|\|\|\|\|\|\|@LB@ UCAAGUG@LB@3'-CGC...CGUUU CAACC...AAG-5'@LB@ \| \|@LB@ 88 80@LB@@LB@@LB@Energy -8.12 kcal/mol@LB@Hybridization Energy -9.1905 kcal/mol@LB@Unfolding Energy - mRNA 0.576286 kcal/mol@LB@Unfolding Energy - sRNA 0.494577 kcal/mol@LB@@LB@Position - mRNA 240 -- 246@LB@Position - sRNA 81 -- 87@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 81 -- 87@LB@
176	0.03112	0.5559	b2536	hcaT	-7.63	0.261550	240 -- 259	67 -- 85	putative 3-phenylpropionic transporter	_____________239__________________260\n_____________\|____________________\|\n5'-UCG...GCUAU_____AUACUU_________CGGCA...GGA-3'\n______________UUCAC______UUUUAGUUA\n______________\|\|\|\|\|______:\|\|\|\|\|\|\|\|\n______________AAGUG______GAAAUCAAU\n3'-CGC...UUUUC_____CAACC__________AAGCU...AAG-5'\n_____________\|____________________\|\n_____________86___________________66\n	253 -- 259	67 -- 73	-10.0019 kcal/mol	1.80629 kcal/mol	0.569003 kcal/mol	239 260@LB@ \| \|@LB@5'-UCG...GCUAU AUACUU CGGCA...GGA-3'@LB@ UUCAC UUUUAGUUA@LB@ \|\|\|\|\| :\|\|\|\|\|\|\|\|@LB@ AAGUG GAAAUCAAU@LB@3'-CGC...UUUUC CAACC AAGCU...AAG-5'@LB@ \| \|@LB@ 86 66@LB@@LB@@LB@Energy -7.63 kcal/mol@LB@Hybridization Energy -10.0019 kcal/mol@LB@Unfolding Energy - mRNA 1.80629 kcal/mol@LB@Unfolding Energy - sRNA 0.569003 kcal/mol@LB@@LB@Position - mRNA 240 -- 259@LB@Position - sRNA 67 -- 85@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 67 -- 73@LB@
13	0.002299	0.5385	b3663	yicN	-11.49	0.058154	241 -- 256	60 -- 78	DUF1198 family protein	_____________240_________________257\n_____________\|___________________\|\n5'-CUU...GUUUG___________________GCUGA...AUC-3'\n______________UGGU__UGGUU_UUCGGGU\n______________\|\|\|:__\|:\|\|\|_\|\|\|\|:\|\|\n______________ACCG__AUCAA_AAGCUCA\n3'-CGC...GUGCA____AA_____U_______ACUCU...AAG-5'\n_____________\|___________________\|\n_____________79__________________59\n	250 -- 256	60 -- 66	-15.2298 kcal/mol	2.94333 kcal/mol	0.797083 kcal/mol	240 257@LB@ \| \|@LB@5'-CUU...GUUUG GCUGA...AUC-3'@LB@ UGGU UGGUU UUCGGGU@LB@ \|\|\|: \|:\|\|\| \|\|\|\|:\|\|@LB@ ACCG AUCAA AAGCUCA@LB@3'-CGC...GUGCA AA U ACUCU...AAG-5'@LB@ \| \|@LB@ 79 59@LB@@LB@@LB@Energy -11.49 kcal/mol@LB@Hybridization Energy -15.2298 kcal/mol@LB@Unfolding Energy - mRNA 2.94333 kcal/mol@LB@Unfolding Energy - sRNA 0.797083 kcal/mol@LB@@LB@Position - mRNA 241 -- 256@LB@Position - sRNA 60 -- 78@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 60 -- 66@LB@
68	0.013	0.5385	b2823	ppdC	-12.74	0.034901	243 -- 262	69 -- 89	putative prepilin peptidase-dependent protein	_____________242___________________263\n_____________\|_____________________\|\n5'-ACG...UGCCG_____A_________GA____GUUGA...UCC-3'\n______________GAGGU__AUGUUGGC__UGGU\n______________:\|:\|\|__\|:\|\|\|\|\|\|__\|:\|\|\n______________UUUCA__UGCAACCG__AUCA\n3'-CGC...GGCGU_____AG________AA____AUAAG...AAG-5'\n_____________\|_____________________\|\n_____________90____________________68\n	250 -- 256	75 -- 81	-18.5568 kcal/mol	5.24273 kcal/mol	0.572143 kcal/mol	242 263@LB@ \| \|@LB@5'-ACG...UGCCG A GA GUUGA...UCC-3'@LB@ GAGGU AUGUUGGC UGGU@LB@ :\|:\|\| \|:\|\|\|\|\|\| \|:\|\|@LB@ UUUCA UGCAACCG AUCA@LB@3'-CGC...GGCGU AG AA AUAAG...AAG-5'@LB@ \| \|@LB@ 90 68@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -18.5568 kcal/mol@LB@Unfolding Energy - mRNA 5.24273 kcal/mol@LB@Unfolding Energy - sRNA 0.572143 kcal/mol@LB@@LB@Position - mRNA 243 -- 262@LB@Position - sRNA 69 -- 89@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 75 -- 81@LB@
23	0.004272	0.5385	b3315	rplV	-10.64	0.081808	245 -- 278	60 -- 90	50S ribosomal subunit protein L22	_____________244________________________________279\n_____________\|__________________________________\|\n5'-CGC...GCUCA__________C___UU___GACCUG_____C___AAGAA...UGG-3'\n______________GAAGGUUCGC_UUG__GCU______AUUCG_GGU\n______________:\|\|:\|\|\|\|:\|_\|\|\|__\|\|\|______\|\|\|\|\|_:\|\|\n______________UUUUCAAGUG_AAC__CGA______UAAGC_UCA\n3'-CGC...AGGCG__________C________AAUCAA_________ACUCU...AAG-5'\n_____________\|__________________________________\|\n_____________91_________________________________59\n	248 -- 254	81 -- 87	-21.0379 kcal/mol	8.68511 kcal/mol	1.70935 kcal/mol	244 279@LB@ \| \|@LB@5'-CGC...GCUCA C UU GACCUG C AAGAA...UGG-3'@LB@ GAAGGUUCGC UUG GCU AUUCG GGU@LB@ :\|\|:\|\|\|\|:\| \|\|\| \|\|\| \|\|\|\|\| :\|\|@LB@ UUUUCAAGUG AAC CGA UAAGC UCA@LB@3'-CGC...AGGCG C AAUCAA ACUCU...AAG-5'@LB@ \| \|@LB@ 91 59@LB@@LB@@LB@Energy -10.64 kcal/mol@LB@Hybridization Energy -21.0379 kcal/mol@LB@Unfolding Energy - mRNA 8.68511 kcal/mol@LB@Unfolding Energy - sRNA 1.70935 kcal/mol@LB@@LB@Position - mRNA 245 -- 278@LB@Position - sRNA 60 -- 90@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 81 -- 87@LB@
96	0.0164	0.5385	b2670	alaE	-9.59	0.124335	245 -- 257	74 -- 86	alanine exporter alanine-inducible stress-responsive	_____________244___________258\n_____________\|_____________\|\n5'-CGG...GAUAC_______A_____GUUUA...UCU-3'\n______________GUUCGCG_UGGUU\n______________\|\|\|\|:\|\|_\|\|\|:\|\n______________CAAGUGC_ACCGA\n3'-CGC...GUUUU_______A_____AAUCA...AAG-5'\n_____________\|_____________\|\n_____________87____________73\n	245 -- 251	80 -- 86	-15.4589 kcal/mol	5.35611 kcal/mol	0.513318 kcal/mol	244 258@LB@ \| \|@LB@5'-CGG...GAUAC A GUUUA...UCU-3'@LB@ GUUCGCG UGGUU@LB@ \|\|\|\|:\|\| \|\|\|:\|@LB@ CAAGUGC ACCGA@LB@3'-CGC...GUUUU A AAUCA...AAG-5'@LB@ \| \|@LB@ 87 73@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -15.4589 kcal/mol@LB@Unfolding Energy - mRNA 5.35611 kcal/mol@LB@Unfolding Energy - sRNA 0.513318 kcal/mol@LB@@LB@Position - mRNA 245 -- 257@LB@Position - sRNA 74 -- 86@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 80 -- 86@LB@
91	0.0156	0.5385	b0881	clpS	-13.09	0.030255	248 -- 265	74 -- 90	regulatory protein for ClpA substrate specificity	_____________247________________266\n_____________\|__________________\|\n5'-GCA...GAAGA___________AC_C___AAAAC...UCA-3'\n______________AAAAGUUCGCG__G_GCU\n______________\|\|\|\|\|\|\|\|:\|\|__\|_\|\|\|\n______________UUUUCAAGUGC__C_CGA\n3'-CGC...AGGCG___________AA_____AAUCA...AAG-5'\n_____________\|__________________\|\n_____________91_________________73\n	252 -- 258	80 -- 86	-16.9257 kcal/mol	3.31752 kcal/mol	0.517587 kcal/mol	247 266@LB@ \| \|@LB@5'-GCA...GAAGA AC C AAAAC...UCA-3'@LB@ AAAAGUUCGCG G GCU@LB@ \|\|\|\|\|\|\|\|:\|\| \| \|\|\|@LB@ UUUUCAAGUGC C CGA@LB@3'-CGC...AGGCG AA AAUCA...AAG-5'@LB@ \| \|@LB@ 91 73@LB@@LB@@LB@Energy -13.09 kcal/mol@LB@Hybridization Energy -16.9257 kcal/mol@LB@Unfolding Energy - mRNA 3.31752 kcal/mol@LB@Unfolding Energy - sRNA 0.517587 kcal/mol@LB@@LB@Position - mRNA 248 -- 265@LB@Position - sRNA 74 -- 90@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 80 -- 86@LB@
179	0.03159	0.5559	b0790	ybhP	-12.03	0.046743	250 -- 264	71 -- 85	endo/exonuclease/phosphatase family protein	_____________249_____________265\n_____________\|_______________\|\n5'-AGU...CAAUA_____AAA_______CCGCG...UUC-3'\n______________UUCAC___GGCUUUA\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\n______________AAGUG___CCGAAAU\n3'-CGC...UUUUC_____CAA_______CAAUA...AAG-5'\n_____________\|_______________\|\n_____________86______________70\n	258 -- 264	71 -- 77	-13.6298 kcal/mol	1.04243 kcal/mol	0.560731 kcal/mol	249 265@LB@ \| \|@LB@5'-AGU...CAAUA AAA CCGCG...UUC-3'@LB@ UUCAC GGCUUUA@LB@ \|\|\|\|\| \|\|\|\|\|\|\|@LB@ AAGUG CCGAAAU@LB@3'-CGC...UUUUC CAA CAAUA...AAG-5'@LB@ \| \|@LB@ 86 70@LB@@LB@@LB@Energy -12.03 kcal/mol@LB@Hybridization Energy -13.6298 kcal/mol@LB@Unfolding Energy - mRNA 1.04243 kcal/mol@LB@Unfolding Energy - sRNA 0.560731 kcal/mol@LB@@LB@Position - mRNA 250 -- 264@LB@Position - sRNA 71 -- 85@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 71 -- 77@LB@
134	0.0229	0.5385	b3729	glmS	-8.05	0.223933	255 -- 266	79 -- 90	L-glutamine:D-fructose-6-phosphate aminotransferase	_____________254__________267\n_____________\|____________\|\n5'-CAG...UUCUU____________CGUCU...GUC-3'\n______________GAAGGUUUACGU\n______________:\|\|:\|\|\|:\|\|\|\|\n______________UUUUCAAGUGCA\n3'-CGC...AGGCG____________ACCGA...AAG-5'\n_____________\|____________\|\n_____________91___________78\n	260 -- 266	79 -- 85	-12.2414 kcal/mol	3.68327 kcal/mol	0.506924 kcal/mol	254 267@LB@ \| \|@LB@5'-CAG...UUCUU CGUCU...GUC-3'@LB@ GAAGGUUUACGU@LB@ :\|\|:\|\|\|:\|\|\|\|@LB@ UUUUCAAGUGCA@LB@3'-CGC...AGGCG ACCGA...AAG-5'@LB@ \| \|@LB@ 91 78@LB@@LB@@LB@Energy -8.05 kcal/mol@LB@Hybridization Energy -12.2414 kcal/mol@LB@Unfolding Energy - mRNA 3.68327 kcal/mol@LB@Unfolding Energy - sRNA 0.506924 kcal/mol@LB@@LB@Position - mRNA 255 -- 266@LB@Position - sRNA 79 -- 90@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 79 -- 85@LB@
22	0.004141	0.5385	b0462	acrB	-13.08	0.030428	256 -- 265	75 -- 84	multidrug efflux system protein	_____________255________266\n_____________\|__________\|\n5'-AAG...UAUCA__________AGGGG...CGC-3'\n______________UCAUGUUGGC\n______________\|\|\|:\|\|\|\|\|\|\n______________AGUGCAACCG\n3'-CGC...UUUCA__________AAAUC...AAG-5'\n_____________\|__________\|\n_____________85_________74\n	259 -- 265	75 -- 81	-16.8887 kcal/mol	3.30616 kcal/mol	0.505918 kcal/mol	255 266@LB@ \| \|@LB@5'-AAG...UAUCA AGGGG...CGC-3'@LB@ UCAUGUUGGC@LB@ \|\|\|:\|\|\|\|\|\|@LB@ AGUGCAACCG@LB@3'-CGC...UUUCA AAAUC...AAG-5'@LB@ \| \|@LB@ 85 74@LB@@LB@@LB@Energy -13.08 kcal/mol@LB@Hybridization Energy -16.8887 kcal/mol@LB@Unfolding Energy - mRNA 3.30616 kcal/mol@LB@Unfolding Energy - sRNA 0.505918 kcal/mol@LB@@LB@Position - mRNA 256 -- 265@LB@Position - sRNA 75 -- 84@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 75 -- 81@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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