Input and runtime details for job 1654565 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1654565 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:23:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:05 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:25:53 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:26:01 CET 2018
	Job Completed		@ Fri Feb 16 22:26:34 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1654565&toolName=CopraRNA ( 30 days expiry )

Description of the job

FnrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
146	0.005657	0.1365	b4322	uxuA	-7.47	0.371307	180 -- 195	52 -- 75	mannonate hydrolase		_____________179______________________196\n_____________\|________________________\|\n5'-CAU...ACCCA________________________AAAUC...AUA-3'\n______________CUAUGU________GAAAGAGGAA\n______________\|\|\|\|\|:________:\|\|\|:\|\|\|\|\|\n______________GAUACG________UUUUUUCCUU\n3'-UUU...AACAC______UCAUUAAG__________CAUUC...ACG-5'\n_____________\|________________________\|\n_____________76_______________________51\n	189 -- 195	52 -- 58	-13.8583 kcal/mol	2.68591 kcal/mol	3.70263 kcal/mol	179 196@LB@ \| \|@LB@5'-CAU...ACCCA AAAUC...AUA-3'@LB@ CUAUGU GAAAGAGGAA@LB@ \|\|\|\|\|: :\|\|\|:\|\|\|\|\|@LB@ GAUACG UUUUUUCCUU@LB@3'-UUU...AACAC UCAUUAAG CAUUC...ACG-5'@LB@ \| \|@LB@ 76 51@LB@@LB@@LB@Energy -7.47 kcal/mol@LB@Hybridization Energy -13.8583 kcal/mol@LB@Unfolding Energy - mRNA 2.68591 kcal/mol@LB@Unfolding Energy - sRNA 3.70263 kcal/mol@LB@@LB@Position - mRNA 180 -- 195@LB@Position - sRNA 52 -- 75@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 52 -- 58@LB@
168	0.006702	0.1406	b2051	wcaH	-7.67	0.342256	86 -- 123	5 -- 40	GDP-mannose mannosyl hydrolase		_____________85_______________________________________124\n_____________\|________________________________________\|\n5'-GCG...ACUGC____U___AC_____UG____A_______UG_________GAAAU...UUG-3'\n______________UGGA_GUG__GCGCC__CAUC_GCUUGGC___GUA_UCAC\n______________\|\|\|\|_\|:\|__:\|\|\|\|__\|\|\|\|_\|\|\|\|\|:\|___\|:\|_\|\|\|\|\n______________ACCU_CGC__UGCGG__GUAG_CGAACUG___CGU_AGUG\n3'-UUU...GUUAU________GU___________________CAA___A____GACG-5'\n_____________\|________________________________________\|\n_____________41_______________________________________4\n	108 -- 114	16 -- 22	-36.6804 kcal/mol	13.756 kcal/mol	15.262 kcal/mol	85 124@LB@ \| \|@LB@5'-GCG...ACUGC U AC UG A UG GAAAU...UUG-3'@LB@ UGGA GUG GCGCC CAUC GCUUGGC GUA UCAC@LB@ \|\|\|\| \|:\| :\|\|\|\| \|\|\|\| \|\|\|\|\|:\| \|:\| \|\|\|\|@LB@ ACCU CGC UGCGG GUAG CGAACUG CGU AGUG@LB@3'-UUU...GUUAU GU CAA A GACG-5'@LB@ \| \|@LB@ 41 4@LB@@LB@@LB@Energy -7.67 kcal/mol@LB@Hybridization Energy -36.6804 kcal/mol@LB@Unfolding Energy - mRNA 13.756 kcal/mol@LB@Unfolding Energy - sRNA 15.262 kcal/mol@LB@@LB@Position - mRNA 86 -- 123@LB@Position - sRNA 5 -- 40@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 16 -- 22@LB@
32	0.0001844	0.0203	b0006	yaaA	-10.88	0.067423	215 -- 223	1 -- 9	peroxide resistance protein lowers intracellular iron		_____________214_______224\n_____________\|_________\|\n5'-UUC...CUUAU_________GAAAA...AGU-3'\n______________UUCACCUGC\n______________\|\|\|\|\|\|\|\|\|\n______________AAGUGGACG\n3'-UUU...AACGU_________-5'\n_____________\|________\|\n_____________10_______1\n	217 -- 223	1 -- 7	-15.2887 kcal/mol	2.04449 kcal/mol	2.37317 kcal/mol	214 224@LB@ \| \|@LB@5'-UUC...CUUAU GAAAA...AGU-3'@LB@ UUCACCUGC@LB@ \|\|\|\|\|\|\|\|\|@LB@ AAGUGGACG@LB@3'-UUU...AACGU -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -10.88 kcal/mol@LB@Hybridization Energy -15.2887 kcal/mol@LB@Unfolding Energy - mRNA 2.04449 kcal/mol@LB@Unfolding Energy - sRNA 2.37317 kcal/mol@LB@@LB@Position - mRNA 215 -- 223@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 1 -- 7@LB@
167	0.006646	0.1402	b0007	yaaJ	-8.17	0.274480	254 -- 261	1 -- 8	putative transporter		_____________253______262\n_____________\|________\|\n5'-CAA...AUGAU________UCUUC...GAU-3'\n______________UUACCUGC\n______________\|:\|\|\|\|\|\|\n______________AGUGGACG\n3'-UUU...ACGUA________-5'\n_____________\|_______\|\n_____________9_______1\n	255 -- 261	1 -- 7	-12.8229 kcal/mol	2.29081 kcal/mol	2.36381 kcal/mol	253 262@LB@ \| \|@LB@5'-CAA...AUGAU UCUUC...GAU-3'@LB@ UUACCUGC@LB@ \|:\|\|\|\|\|\|@LB@ AGUGGACG@LB@3'-UUU...ACGUA -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -8.17 kcal/mol@LB@Hybridization Energy -12.8229 kcal/mol@LB@Unfolding Energy - mRNA 2.29081 kcal/mol@LB@Unfolding Energy - sRNA 2.36381 kcal/mol@LB@@LB@Position - mRNA 254 -- 261@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 1 -- 7@LB@
156	0.006066	0.137	b0005	yaaX	-9.16	0.170742	96 -- 118	53 -- 73	DUF2502 family putative periplasmic protein	b2390	_____________95______________________119\n_____________\|_______________________\|\n5'-UCU...AAUAA_________C_______UU____UUGCG...AAU-3'\n______________AUGCGGUAA_UUAGAGA__AGGA\n______________\|\|\|\|:\|\|\|\|_\|:\|:\|:\|__\|\|\|\|\n______________UACGUCAUU_AGUUUUU__UCCU\n3'-UUU...CACGA_________A_____________UCAUU...ACG-5'\n_____________\|_______________________\|\n_____________74______________________52\n	98 -- 104	65 -- 71	-18.9701 kcal/mol	6.27714 kcal/mol	3.52907 kcal/mol	95 119@LB@ \| \|@LB@5'-UCU...AAUAA C UU UUGCG...AAU-3'@LB@ AUGCGGUAA UUAGAGA AGGA@LB@ \|\|\|\|:\|\|\|\| \|:\|:\|:\| \|\|\|\|@LB@ UACGUCAUU AGUUUUU UCCU@LB@3'-UUU...CACGA A UCAUU...ACG-5'@LB@ \| \|@LB@ 74 52@LB@@LB@@LB@Energy -9.16 kcal/mol@LB@Hybridization Energy -18.9701 kcal/mol@LB@Unfolding Energy - mRNA 6.27714 kcal/mol@LB@Unfolding Energy - sRNA 3.52907 kcal/mol@LB@@LB@Position - mRNA 96 -- 118@LB@Position - sRNA 53 -- 73@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 65 -- 71@LB@
89	0.002455	0.09689	b0466	ybaM	-10.99	0.063193	248 -- 263	68 -- 82	DUF2496 family protein		_____________247______________264\n_____________\|________________\|\n5'-GCA...GAUUU__________C__G__GAAAA...CGC-3'\n______________GAUUGUGCUG_UG_AG\n______________:\|\|\|\|\|\|\|\|:_\|\|_\|\|\n______________UUAACACGAU_AC_UC\n3'-UUU...CUUAG_____________G__AUUAA...ACG-5'\n_____________\|________________\|\n_____________83_______________67\n	251 -- 257	73 -- 79	-16.1643 kcal/mol	2.45269 kcal/mol	2.72435 kcal/mol	247 264@LB@ \| \|@LB@5'-GCA...GAUUU C G GAAAA...CGC-3'@LB@ GAUUGUGCUG UG AG@LB@ :\|\|\|\|\|\|\|\|: \|\| \|\|@LB@ UUAACACGAU AC UC@LB@3'-UUU...CUUAG G AUUAA...ACG-5'@LB@ \| \|@LB@ 83 67@LB@@LB@@LB@Energy -10.99 kcal/mol@LB@Hybridization Energy -16.1643 kcal/mol@LB@Unfolding Energy - mRNA 2.45269 kcal/mol@LB@Unfolding Energy - sRNA 2.72435 kcal/mol@LB@@LB@Position - mRNA 248 -- 263@LB@Position - sRNA 68 -- 82@LB@Position Seed - mRNA 251 -- 257@LB@Position Seed - sRNA 73 -- 79@LB@
126	0.004539	0.1258	b0546	ybcM	-11.00	0.062763	188 -- 220	45 -- 91	DLP12 prophage; putative DNA-binding transcriptional regulator	b1499 b0564 b3515 b4116 b0566 b3516	_____________187_____________________________________________221\n_____________\|_______________________________________________\|\n5'-GCU...AUAAA___G__UGA__GUA_________A_______________________UAACU...GUC-3'\n______________GUA_GG___GA___UGCUG_GCA______________GAGGUAAGAC\n______________\|\|\|_\|:___:\|___\|\|\|\|:_\|\|\|______________\|\|:\|\|\|\|\|\|\|\n______________CAU_CU___UU___ACGAU_CGU______________CUUCAUUCUG\n3'-UUU...CGCAG___G__UAG__AAC_____A___CAUUAAGUUUUUUC__________UUAUA...ACG-5'\n_____________\|_______________________________________________\|\n_____________92______________________________________________44\n	214 -- 220	45 -- 51	-23.4531 kcal/mol	6.72952 kcal/mol	5.72765 kcal/mol	187 221@LB@ \| \|@LB@5'-GCU...AUAAA G UGA GUA A UAACU...GUC-3'@LB@ GUA GG GA UGCUG GCA GAGGUAAGAC@LB@ \|\|\| \|: :\| \|\|\|\|: \|\|\| \|\|:\|\|\|\|\|\|\|@LB@ CAU CU UU ACGAU CGU CUUCAUUCUG@LB@3'-UUU...CGCAG G UAG AAC A CAUUAAGUUUUUUC UUAUA...ACG-5'@LB@ \| \|@LB@ 92 44@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -23.4531 kcal/mol@LB@Unfolding Energy - mRNA 6.72952 kcal/mol@LB@Unfolding Energy - sRNA 5.72765 kcal/mol@LB@@LB@Position - mRNA 188 -- 220@LB@Position - sRNA 45 -- 91@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 45 -- 51@LB@
69	0.001222	0.06239	b0699	ybfA	-13.19	0.016050	183 -- 205	37 -- 62	DUF2517 family protein		_____________182________________________206\n_____________\|__________________________\|\n5'-AUG...AGAUA____________AAA___________ACUCU...GGA-3'\n______________CAAAAGGGGGAG______AGUAUGGA\n______________\|\|\|\|\|::\|\|:\|\|______\|:\|\|\|\|\|\|\n______________GUUUUUUCCUUC______UUAUACCU\n3'-UUU...AUUAA____________AUUCUG________CGCGU...ACG-5'\n_____________\|__________________________\|\n_____________63_________________________36\n	199 -- 205	37 -- 43	-21.7 kcal/mol	3.27833 kcal/mol	5.2343 kcal/mol	182 206@LB@ \| \|@LB@5'-AUG...AGAUA AAA ACUCU...GGA-3'@LB@ CAAAAGGGGGAG AGUAUGGA@LB@ \|\|\|\|\|::\|\|:\|\| \|:\|\|\|\|\|\|@LB@ GUUUUUUCCUUC UUAUACCU@LB@3'-UUU...AUUAA AUUCUG CGCGU...ACG-5'@LB@ \| \|@LB@ 63 36@LB@@LB@@LB@Energy -13.19 kcal/mol@LB@Hybridization Energy -21.7 kcal/mol@LB@Unfolding Energy - mRNA 3.27833 kcal/mol@LB@Unfolding Energy - sRNA 5.2343 kcal/mol@LB@@LB@Position - mRNA 183 -- 205@LB@Position - sRNA 37 -- 62@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 37 -- 43@LB@
62	0.001027	0.05816	b0717	ybgP	-6.75	0.488398	48 -- 57	53 -- 62	putative periplasmic pilin chaperone	b3047	_____________47_________58\n_____________\|__________\|\n5'-GCG...GUUGG__________CAUGC...CCA-3'\n______________CGAGGAAGGA\n______________\|:\|::\|\|\|\|\|\n______________GUUUUUUCCU\n3'-UUU...AUUAA__________UCAUU...ACG-5'\n_____________\|__________\|\n_____________63_________52\n	51 -- 57	53 -- 59	-10.1576 kcal/mol	0.982686 kcal/mol	2.4254 kcal/mol	47 58@LB@ \| \|@LB@5'-GCG...GUUGG CAUGC...CCA-3'@LB@ CGAGGAAGGA@LB@ \|:\|::\|\|\|\|\|@LB@ GUUUUUUCCU@LB@3'-UUU...AUUAA UCAUU...ACG-5'@LB@ \| \|@LB@ 63 52@LB@@LB@@LB@Energy -6.75 kcal/mol@LB@Hybridization Energy -10.1576 kcal/mol@LB@Unfolding Energy - mRNA 0.982686 kcal/mol@LB@Unfolding Energy - sRNA 2.4254 kcal/mol@LB@@LB@Position - mRNA 48 -- 57@LB@Position - sRNA 53 -- 62@LB@Position Seed - mRNA 51 -- 57@LB@Position Seed - sRNA 53 -- 59@LB@
132	0.004965	0.1313	b0876	ybjD	-10.35	0.091015	20 -- 74	49 -- 93	putative OLD family ATP-dependent endonuclease; DUF2813 family protein		_____________19______________________________________________________75\n_____________\|_______________________________________________________\|\n5'-CGU...AUUUA__A___U_____U___AUUU_____UUUU_____ACUUA____C___________UAACG...GGG-3'\n______________UC_UGC_AAUUA_UUG____UGUUG____UGCAG_____UCAG_AAGAGGGAGUA\n______________\|\|_\|:\|_\|\|\|:\|_\|\|\|____\|\|:\|:____\|\|\|\|\|_____\|\|\|:_\|\|:\|\|\|:\|\|\|\|\n______________AG_AUG_UUAGU_AAC____ACGAU____ACGUC_____AGUU_UUUUCCUUCAU\n3'-UUU...GCCGC__C___C_____U_____________________AUUA_________________UCUGU...ACG-5'\n_____________\|_______________________________________________________\|\n_____________94______________________________________________________48\n	68 -- 74	49 -- 55	-28.5607 kcal/mol	13.348 kcal/mol	4.86987 kcal/mol	19 75@LB@ \| \|@LB@5'-CGU...AUUUA A U U AUUU UUUU ACUUA C UAACG...GGG-3'@LB@ UC UGC AAUUA UUG UGUUG UGCAG UCAG AAGAGGGAGUA@LB@ \|\| \|:\| \|\|\|:\| \|\|\| \|\|:\|: \|\|\|\|\| \|\|\|: \|\|:\|\|\|:\|\|\|\|@LB@ AG AUG UUAGU AAC ACGAU ACGUC AGUU UUUUCCUUCAU@LB@3'-UUU...GCCGC C C U AUUA UCUGU...ACG-5'@LB@ \| \|@LB@ 94 48@LB@@LB@@LB@Energy -10.35 kcal/mol@LB@Hybridization Energy -28.5607 kcal/mol@LB@Unfolding Energy - mRNA 13.348 kcal/mol@LB@Unfolding Energy - sRNA 4.86987 kcal/mol@LB@@LB@Position - mRNA 20 -- 74@LB@Position - sRNA 49 -- 93@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 49 -- 55@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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