Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
111	0.0122	0.3575	b1813	nudL	-9.44	0.347588	99 -- 131	174 -- 206	putative CoA pyrophosphohydrolase weak 3-phosphohydroxypyruvate phosphatase	_____________98________________________________132\n_____________\|_________________________________\|\n5'-CCU...UCUGC_____G_____UG__A__GU________GC___GAAGA...CUG-3'\n______________UCUGC_GGCGG__GA_UU__CGCCGAUA__CAG\n______________\|\|\|\|\|_\|\|\|\|\|__\|\|_\|\|__\|:\|\|:\|\|\|__\|\|\|\n______________AGACG_CCGCC__CU_AA__GUGGUUAU__GUC\n3'-UUU...AUAUU_____A_____CA__A__AU________GA___AGUGU...UAG-5'\n_____________\|_________________________________\|\n_____________207_______________________________173\n	120 -- 126	179 -- 185	-36.2385 kcal/mol	15.3331 kcal/mol	14.8345 kcal/mol	98 132@LB@ \| \|@LB@5'-CCU...UCUGC G UG A GU GC GAAGA...CUG-3'@LB@ UCUGC GGCGG GA UU CGCCGAUA CAG@LB@ \|\|\|\|\| \|\|\|\|\| \|\| \|\| \|:\|\|:\|\|\| \|\|\|@LB@ AGACG CCGCC CU AA GUGGUUAU GUC@LB@3'-UUU...AUAUU A CA A AU GA AGUGU...UAG-5'@LB@ \| \|@LB@ 207 173@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -36.2385 kcal/mol@LB@Unfolding Energy - mRNA 15.3331 kcal/mol@LB@Unfolding Energy - sRNA 14.8345 kcal/mol@LB@@LB@Position - mRNA 99 -- 131@LB@Position - sRNA 174 -- 206@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 179 -- 185@LB@
112	0.01232	0.3575	b1540	rspR	-15.19	0.024761	13 -- 44	171 -- 202	transcriptional repressor for rspAB	_____________12_________________________________________________________45\n_____________\|__________________________________________________________\|\n5'-CAG...GCCGU__________UCA_______GCC______C___A____CAAUACCCUGGAA_______CGGUU...GCA-3'\n______________CUGGUGGGUG_____ACGCU___UGCUCA_GUC_AUAU_____________AUGAUGC\n______________\|\|\|\|:\|\|\|\|\|_____\|\|:\|:___\|:\|\|\|\|_\|\|\|_\|:\|:_____________\|\|\|\|\|\|\|\n______________GACCGCCCAC_____UGUGG___AUGAGU_CAG_UGUG_____________UACUACG\n3'-UUU...UAGAC__________UAAAA_____UU____________________________________UCCGU...UAG-5'\n_____________\|__________________________________________________________\|\n_____________203________________________________________________________170\n	62 -- 68	161 -- 167	-40.6001 kcal/mol	15.8189 kcal/mol	17.3482 kcal/mol	12 45@LB@ \| \|@LB@5'-CAG...GCCGU UCA GCC C A CAAUACCCUGGAA CGGUU...GCA-3'@LB@ CUGGUGGGUG ACGCU UGCUCA GUC AUAU AUGAUGC@LB@ \|\|\|\|:\|\|\|\|\| \|\|:\|: \|:\|\|\|\| \|\|\| \|:\|: \|\|\|\|\|\|\|@LB@ GACCGCCCAC UGUGG AUGAGU CAG UGUG UACUACG@LB@3'-UUU...UAGAC UAAAA UU UCCGU...UAG-5'@LB@ \| \|@LB@ 203 170@LB@@LB@@LB@Energy -15.19 kcal/mol@LB@Hybridization Energy -40.6001 kcal/mol@LB@Unfolding Energy - mRNA 15.8189 kcal/mol@LB@Unfolding Energy - sRNA 17.3482 kcal/mol@LB@@LB@Position - mRNA 13 -- 44@LB@Position - sRNA 171 -- 202@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 161 -- 167@LB@
113	0.01268	0.3575	b2066	udk	-9.33	0.364673	240 -- 253	17 -- 31	uridine/cytidine kinase	_____________239________________254\n_____________\|__________________\|\n5'-CGC...UUCGU_________C__AA____CAGGU...GUG-3'\n______________CGCCCCCUU_CU__CAUC\n______________\|:\|\|\|\|\|\|\|_\|\|__\|\|\|\|\n______________GUGGGGGAA_GA__GUAG\n3'-UUU...ACCCC_________C__A_____-5'\n_____________\|_________________\|\n_____________32________________17\n	161 -- 167	9 -- 15	-20.5437 kcal/mol	3.26859 kcal/mol	7.16879 kcal/mol	239 254@LB@ \| \|@LB@5'-CGC...UUCGU C AA CAGGU...GUG-3'@LB@ CGCCCCCUU CU CAUC@LB@ \|:\|\|\|\|\|\|\| \|\| \|\|\|\|@LB@ GUGGGGGAA GA GUAG@LB@3'-UUU...ACCCC C A -5'@LB@ \| \|@LB@ 32 17@LB@@LB@@LB@Energy -9.33 kcal/mol@LB@Hybridization Energy -20.5437 kcal/mol@LB@Unfolding Energy - mRNA 3.26859 kcal/mol@LB@Unfolding Energy - sRNA 7.16879 kcal/mol@LB@@LB@Position - mRNA 240 -- 253@LB@Position - sRNA 17 -- 31@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 9 -- 15@LB@
114	0.01268	0.3575	b0888	trxB	-13.24	0.061992	5 -- 14	83 -- 92	thioredoxin reductase FAD/NAD(P)-binding	_____________4__________15\n_____________\|__________\|\n_______5'-AACA__________UUCAU...UGC-3'\n______________GGGGCGUUUA\n______________\|\|\|:\|\|:\|\|\|\n______________CCCUGCGAAU\n3'-UUU...CAACA__________UGGUU...UAG-5'\n_____________\|__________\|\n_____________93_________82\n	7 -- 13	84 -- 90	-15.9307 kcal/mol	0.512019 kcal/mol	6.69187 kcal/mol	4 15@LB@ \| \|@LB@ 5'-AACA UUCAU...UGC-3'@LB@ GGGGCGUUUA@LB@ \|\|\|:\|\|:\|\|\|@LB@ CCCUGCGAAU@LB@3'-UUU...CAACA UGGUU...UAG-5'@LB@ \| \|@LB@ 93 82@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -15.9307 kcal/mol@LB@Unfolding Energy - mRNA 0.512019 kcal/mol@LB@Unfolding Energy - sRNA 6.69187 kcal/mol@LB@@LB@Position - mRNA 5 -- 14@LB@Position - sRNA 83 -- 92@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 84 -- 90@LB@
115	0.01273	0.3575	b3440	yhhX	-9.67	0.316530	203 -- 215	123 -- 136	putative oxidoreductase	_____________202______________________________216\n_____________\|________________________________\|\n5'-GAC...CGUAU_______AC____AG____A__U____U____CGCAUA-3'\n______________GUACUUA__CGCA___GAU_GC_GGCA_GUCG\n______________:\|\|\|\|:\|__\|:\|\|___\|\|\|_\|\|_\|\|\|\|_\|\|\|:\n______________UAUGAGU__GUGU___CUA_CG_CCGU_CAGU\n3'-UUU...GUGGU_______CA____GUA______U____U____UGAAA...UAG-5'\n_____________\|________________________________\|\n_____________137______________________________122\n	264 -- 270	175 -- 181	-25.3028 kcal/mol	7.53441 kcal/mol	8.15082 kcal/mol	202 216@LB@ \| \|@LB@5'-GAC...CGUAU AC AG A U U CGCAUA-3'@LB@ GUACUUA CGCA GAU GC GGCA GUCG@LB@ :\|\|\|\|:\| \|:\|\| \|\|\| \|\| \|\|\|\| \|\|\|:@LB@ UAUGAGU GUGU CUA CG CCGU CAGU@LB@3'-UUU...GUGGU CA GUA U U UGAAA...UAG-5'@LB@ \| \|@LB@ 137 122@LB@@LB@@LB@Energy -9.67 kcal/mol@LB@Hybridization Energy -25.3028 kcal/mol@LB@Unfolding Energy - mRNA 7.53441 kcal/mol@LB@Unfolding Energy - sRNA 8.15082 kcal/mol@LB@@LB@Position - mRNA 203 -- 215@LB@Position - sRNA 123 -- 136@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 175 -- 181@LB@
116	0.01276	0.3575	b2989	yghU	-14.25	0.038539	247 -- 281	170 -- 208	putative S-transferase	_____________246______________________________________282\n_____________\|________________________________________\|\n5'-UUA...GGAUA____C________C_C_UU________________A____CCGGU...CGC-3'\n______________AAUC_GCUGGCGG_G_G______CGCCAAUA_UCA_UCGC\n______________\|\|\|\|_\|\|\|\|\|\|\|\|_\|_\|______\|:\|\|\|\|\|\|_\|\|\|_\|\|:\|\n______________UUAG_CGACCGCC_C_C______GUGGUUAU_AGU_AGUG\n3'-UUU...CCAUA____A__________A_UAAAAU________G___C____UGUAC...UAG-5'\n_____________\|________________________________________\|\n_____________209______________________________________169\n	267 -- 273	179 -- 185	-35.9489 kcal/mol	9.63265 kcal/mol	15.256 kcal/mol	246 282@LB@ \| \|@LB@5'-UUA...GGAUA C C C UU A CCGGU...CGC-3'@LB@ AAUC GCUGGCGG G G CGCCAAUA UCA UCGC@LB@ \|\|\|\| \|\|\|\|\|\|\|\| \| \| \|:\|\|\|\|\|\| \|\|\| \|\|:\|@LB@ UUAG CGACCGCC C C GUGGUUAU AGU AGUG@LB@3'-UUU...CCAUA A A UAAAAU G C UGUAC...UAG-5'@LB@ \| \|@LB@ 209 169@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -35.9489 kcal/mol@LB@Unfolding Energy - mRNA 9.63265 kcal/mol@LB@Unfolding Energy - sRNA 15.256 kcal/mol@LB@@LB@Position - mRNA 247 -- 281@LB@Position - sRNA 170 -- 208@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 179 -- 185@LB@
117	0.01283	0.3575	b3096	mzrA	-10.41	0.230387	15 -- 55	168 -- 208	modulator of EnvZ/OmpR regulon	_____________14________________________________________________________56\n_____________\|_________________________________________________________\|\n_____5'-UCAACU____G____________________________GAUCCUGA___CU__G________GCUCG...AGC-3'\n______________GGAU_AGCGG______UCUGCU_GUGGGU_AUU________CAA__CU_GGUUACAU\n______________::\|:_\|\|\|:\|______\|\|\|\|\|\|_\|:\|\|\|\|_\|\|\|________\|\|\|__\|\|_:\|\|:\|\|\|:\n______________UUUG_UCGUC______AGACGA_CGCCCA_UAA________GUU__GA_UCAGUGUG\n______3'-UUUUU____G_____CAUAUU______C______C___AAUGUG_____AU__G________UACUA...UAG-5'\n_____________\|_________________________________________________________\|\n_____________209_______________________________________________________167\n	49 -- 55	168 -- 174	-40.4023 kcal/mol	11.8856 kcal/mol	18.3074 kcal/mol	14 56@LB@ \| \|@LB@ 5'-UCAACU G GAUCCUGA CU G GCUCG...AGC-3'@LB@ GGAU AGCGG UCUGCU GUGGGU AUU CAA CU GGUUACAU@LB@ ::\|: \|\|\|:\| \|\|\|\|\|\| \|:\|\|\|\| \|\|\| \|\|\| \|\| :\|\|:\|\|\|:@LB@ UUUG UCGUC AGACGA CGCCCA UAA GUU GA UCAGUGUG@LB@ 3'-UUUUU G CAUAUU C C AAUGUG AU G UACUA...UAG-5'@LB@ \| \|@LB@ 209 167@LB@@LB@@LB@Energy -10.41 kcal/mol@LB@Hybridization Energy -40.4023 kcal/mol@LB@Unfolding Energy - mRNA 11.8856 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 15 -- 55@LB@Position - sRNA 168 -- 208@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 168 -- 174@LB@
118	0.01293	0.3575	b1598	ydgD	-17.08	0.010207	53 -- 140	115 -- 186	putative peptidase	_____________52______________________________________________________________________141\n_____________\|_______________________________________________________________________\|\n5'-CUU...AUCCU_____UAUG__________UACC__CC___UUUAU_____CU______A____UCUGAUUUUAC_______UUCAU...AGG-3'\n______________UAUUU____CGCAC_UGAU____GC__GUU_____CUUUC__GAUUGU_GUUA___________UCCCACU\n______________\|\|:\|:____\|:\|\|\|_\|\|\|\|____\|\|__\|\|:_____\|\|\|\|\|__:\|\|\|\|:_\|\|:\|___________\|\|\|\|\|\|\|\n______________AUGAG____GUGUG_ACUA____CG__CAG_____GAAAG__UUAACG_CAGU___________AGGGUGA\n3'-UUU...UGGUU_____UCA______U____CGUC__UU___UU________UC______C______________________CGUAG...UAG-5'\n_____________\|_______________________________________________________________________\|\n_____________187_____________________________________________________________________114\n	122 -- 128	125 -- 131	-34.2678 kcal/mol	6.55961 kcal/mol	16.6417 kcal/mol	52 141@LB@ \| \|@LB@5'-CUU...AUCCU UAUG UACC CC UUUAU CU A UCUGAUUUUAC UUCAU...AGG-3'@LB@ UAUUU CGCAC UGAU GC GUU CUUUC GAUUGU GUUA UCCCACU@LB@ \|\|:\|: \|:\|\|\| \|\|\|\| \|\| \|\|: \|\|\|\|\| :\|\|\|\|: \|\|:\| \|\|\|\|\|\|\|@LB@ AUGAG GUGUG ACUA CG CAG GAAAG UUAACG CAGU AGGGUGA@LB@3'-UUU...UGGUU UCA U CGUC UU UU UC C CGUAG...UAG-5'@LB@ \| \|@LB@ 187 114@LB@@LB@@LB@Energy -17.08 kcal/mol@LB@Hybridization Energy -34.2678 kcal/mol@LB@Unfolding Energy - mRNA 6.55961 kcal/mol@LB@Unfolding Energy - sRNA 16.6417 kcal/mol@LB@@LB@Position - mRNA 53 -- 140@LB@Position - sRNA 115 -- 186@LB@Position Seed - mRNA 122 -- 128@LB@Position Seed - sRNA 125 -- 131@LB@
119	0.013	0.3575	b0801	ybiC	-16.26	0.015008	158 -- 253	108 -- 187	putative dehydrogenase	_____________157____________________________________________________________________________________________254\n_____________\|______________________________________________________________________________________________\|\n5'-GCU...AUAAA_____AA__AA______ACAUAAA_____________CAU_____A__________G_UUUGAUGCUCAGAC_____________UA_______UGUAU...UUG-3'\n______________AAUAC__CA__CACAUA_______CAGG__AGUUAAC____GGAA__GUGGUCAUC_C______________GCUGCA_CAGUUU__UUCAGGC\n______________\|\|\|\|\|__\|\|__\|\|\|\|:\|_______\|\|\|\|__\|\|\|:\|\|\|____:\|\|\|__\|:\|\|\|\|\|\|\|_\|______________:\|\|\|\|\|_\|\|\|\|::__\|\|\|\|:\|\|\n______________UUAUG__GU__GUGUGU_______GUCC__UCAGUUG____UCUU__CGCCAGUAG_G______________UGACGU_GUCAGG__AAGUUCG\n3'-UUU...UGUGG_____A___CA______ACUAC______GU_______AAAG____AA_________G_____________________A_______________UACCA...UAG-5'\n_____________\|______________________________________________________________________________________________\|\n_____________188____________________________________________________________________________________________107\n	247 -- 253	108 -- 114	-56.5044 kcal/mol	18.8914 kcal/mol	26.5178 kcal/mol	157 254@LB@ \| \|@LB@5'-GCU...AUAAA AA AA ACAUAAA CAU A G UUUGAUGCUCAGAC UA UGUAU...UUG-3'@LB@ AAUAC CA CACAUA CAGG AGUUAAC GGAA GUGGUCAUC C GCUGCA CAGUUU UUCAGGC@LB@ \|\|\|\|\| \|\| \|\|\|\|:\| \|\|\|\| \|\|\|:\|\|\| :\|\|\| \|:\|\|\|\|\|\|\| \| :\|\|\|\|\| \|\|\|\|:: \|\|\|\|:\|\|@LB@ UUAUG GU GUGUGU GUCC UCAGUUG UCUU CGCCAGUAG G UGACGU GUCAGG AAGUUCG@LB@3'-UUU...UGUGG A CA ACUAC GU AAAG AA G A UACCA...UAG-5'@LB@ \| \|@LB@ 188 107@LB@@LB@@LB@Energy -16.26 kcal/mol@LB@Hybridization Energy -56.5044 kcal/mol@LB@Unfolding Energy - mRNA 18.8914 kcal/mol@LB@Unfolding Energy - sRNA 26.5178 kcal/mol@LB@@LB@Position - mRNA 158 -- 253@LB@Position - sRNA 108 -- 187@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 108 -- 114@LB@
120	0.01317	0.3575	b1158	pinE	-11.56	0.136715	30 -- 59	115 -- 145	e14 prophage; site-specific DNA recombinase	_____________29_____________________60\n_____________\|______________________\|\n5'-AGC...CCGGG______________GUA_____GAGCC...ACA-3'\n______________ACACCAA____CAG___AUGCA\n______________\|\|\|\|\|\|\|____\|\|\|___\|::\|\|\n______________UGUGGUU____GUC___UGUGU\n3'-UUU...UAAAA_______AUGA___AG______ACUAC...UAG-5'\n_____________\|______________________\|\n_____________146____________________114\n	98 -- 104	181 -- 187	-13.8739 kcal/mol	4.34554 kcal/mol	5.05492 kcal/mol	29 60@LB@ \| \|@LB@5'-AGC...CCGGG GUA GAGCC...ACA-3'@LB@ ACACCAA CAG AUGCA@LB@ \|\|\|\|\|\|\| \|\|\| \|::\|\|@LB@ UGUGGUU GUC UGUGU@LB@3'-UUU...UAAAA AUGA AG ACUAC...UAG-5'@LB@ \| \|@LB@ 146 114@LB@@LB@@LB@Energy -11.56 kcal/mol@LB@Hybridization Energy -13.8739 kcal/mol@LB@Unfolding Energy - mRNA 4.34554 kcal/mol@LB@Unfolding Energy - sRNA 5.05492 kcal/mol@LB@@LB@Position - mRNA 30 -- 59@LB@Position - sRNA 115 -- 145@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 181 -- 187@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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