Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
11	0.0006963	0.1842	b1739	osmE	-13.14	0.065080	250 -- 260	194 -- 204	osmotically-inducible lipoprotein		_____________249_________261\n_____________\|___________\|\n5'-GAA...AACCA___________UGUAC...AGC-3'\n______________UGCUGGCGGGU\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________ACGACCGCCCA\n3'-UUU...AUUAG___________CUAAA...UAG-5'\n_____________\|___________\|\n_____________205_________193\n	254 -- 260	194 -- 200	-22.742 kcal/mol	5.67651 kcal/mol	8.00138 kcal/mol	249 261@LB@ \| \|@LB@5'-GAA...AACCA UGUAC...AGC-3'@LB@ UGCUGGCGGGU@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ ACGACCGCCCA@LB@3'-UUU...AUUAG CUAAA...UAG-5'@LB@ \| \|@LB@ 205 193@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -22.742 kcal/mol@LB@Unfolding Energy - mRNA 5.67651 kcal/mol@LB@Unfolding Energy - sRNA 8.00138 kcal/mol@LB@@LB@Position - mRNA 250 -- 260@LB@Position - sRNA 194 -- 204@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 194 -- 200@LB@
12	0.0007005	0.1842	b1101	ptsG	-16.86	0.011310	173 -- 192	168 -- 187	fused glucose-specific PTS enzymes: IIB component/IIC component		_____________172__________________193\n_____________\|____________________\|\n5'-CAU...AAAAA_____C________GA____UCUCA...GUU-3'\n______________GCACC_AUACUCAG__GCAC\n______________:\|\|\|\|_\|\|\|\|\|\|\|\|__:\|\|\|\n______________UGUGG_UAUGAGUC__UGUG\n3'-UUU...UAAAA_____U________AG____UACUA...UAG-5'\n_____________\|____________________\|\n_____________188__________________167\n	180 -- 186	174 -- 180	-27.3381 kcal/mol	3.90127 kcal/mol	4.88067 kcal/mol	172 193@LB@ \| \|@LB@5'-CAU...AAAAA C GA UCUCA...GUU-3'@LB@ GCACC AUACUCAG GCAC@LB@ :\|\|\|\| \|\|\|\|\|\|\|\| :\|\|\|@LB@ UGUGG UAUGAGUC UGUG@LB@3'-UUU...UAAAA U AG UACUA...UAG-5'@LB@ \| \|@LB@ 188 167@LB@@LB@@LB@Energy -16.86 kcal/mol@LB@Hybridization Energy -27.3381 kcal/mol@LB@Unfolding Energy - mRNA 3.90127 kcal/mol@LB@Unfolding Energy - sRNA 4.88067 kcal/mol@LB@@LB@Position - mRNA 173 -- 192@LB@Position - sRNA 168 -- 187@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 174 -- 180@LB@
13	0.0007116	0.1842	b3052	hldE	-13.53	0.054228	111 -- 134	141 -- 169	heptose 7-phosphate kinase and heptose 1-phosphate adenyltransferase		_____________110______________135\n_____________\|________________\|\n5'-GCG...CGUGA________________CUGGC...CGC-3'\n______________AC_UGGUGCGGGCAAG\n______________\|\|_\|\|:\|\|\|:\|\|\|\|\|\|\n______________UG_ACUACGUCCGUUC\n3'-UUU...CAGUG__U_____________AGUUG...UAG-5'\n_____________\|________________\|\n_____________170______________140\n	119 -- 125	154 -- 160	-22.7709 kcal/mol	5.4675 kcal/mol	7.07904 kcal/mol	110 135@LB@ \| \|@LB@5'-GCG...CGUGA CUGGC...CGC-3'@LB@ AC UGGUGCGGGCAAG@LB@ \|\| \|\|:\|\|\|:\|\|\|\|\|\|@LB@ UG ACUACGUCCGUUC@LB@3'-UUU...CAGUG U AGUUG...UAG-5'@LB@ \| \|@LB@ 170 140@LB@@LB@@LB@Energy -13.53 kcal/mol@LB@Hybridization Energy -22.7709 kcal/mol@LB@Unfolding Energy - mRNA 5.4675 kcal/mol@LB@Unfolding Energy - sRNA 7.07904 kcal/mol@LB@@LB@Position - mRNA 111 -- 134@LB@Position - sRNA 141 -- 169@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 154 -- 160@LB@
14	0.00083	0.1886	b2721	hycE	-15.91	0.017607	169 -- 185	190 -- 205	hydrogenase 3 large subunit		_____________168_______________186\n_____________\|_________________\|\n5'-UUC...UCUCC__________C______AAAGA...UGA-3'\n______________UUGCUGGCGG_GUGAUU\n______________:\|\|\|\|\|\|\|\|\|_\|\|\|\|\|\|\n______________GACGACCGCC_CACUAA\n3'-UUU...UAUUA_________________AAUGU...UAG-5'\n_____________\|_________________\|\n_____________206_______________189\n	172 -- 178	196 -- 202	-26.9802 kcal/mol	6.4269 kcal/mol	9.06848 kcal/mol	168 186@LB@ \| \|@LB@5'-UUC...UCUCC C AAAGA...UGA-3'@LB@ UUGCUGGCGG GUGAUU@LB@ :\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|@LB@ GACGACCGCC CACUAA@LB@3'-UUU...UAUUA AAUGU...UAG-5'@LB@ \| \|@LB@ 206 189@LB@@LB@@LB@Energy -15.91 kcal/mol@LB@Hybridization Energy -26.9802 kcal/mol@LB@Unfolding Energy - mRNA 6.4269 kcal/mol@LB@Unfolding Energy - sRNA 9.06848 kcal/mol@LB@@LB@Position - mRNA 169 -- 185@LB@Position - sRNA 190 -- 205@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 196 -- 202@LB@
15	0.0008498	0.1886	b4254	argI	-15.11	0.025648	62 -- 84	167 -- 185	ornithine carbamoyltransferase 1	b0273	_____________61______________________85\n_____________\|_______________________\|\n5'-UUC...GUCCU__________UUAUU________CCACU...AUA-3'\n______________GCCGAUACUC_____GUCACACA\n______________:\|\|:\|\|\|\|\|\|_____\|\|\|\|\|\|\|\|\n______________UGGUUAUGAG_____CAGUGUGU\n3'-UUU...AAAUG__________U____________ACUAC...UAG-5'\n_____________\|_______________________\|\n_____________186_____________________166\n	78 -- 84	167 -- 173	-24.735 kcal/mol	3.62809 kcal/mol	5.02083 kcal/mol	61 85@LB@ \| \|@LB@5'-UUC...GUCCU UUAUU CCACU...AUA-3'@LB@ GCCGAUACUC GUCACACA@LB@ :\|\|:\|\|\|\|\|\| \|\|\|\|\|\|\|\|@LB@ UGGUUAUGAG CAGUGUGU@LB@3'-UUU...AAAUG U ACUAC...UAG-5'@LB@ \| \|@LB@ 186 166@LB@@LB@@LB@Energy -15.11 kcal/mol@LB@Hybridization Energy -24.735 kcal/mol@LB@Unfolding Energy - mRNA 3.62809 kcal/mol@LB@Unfolding Energy - sRNA 5.02083 kcal/mol@LB@@LB@Position - mRNA 62 -- 84@LB@Position - sRNA 167 -- 185@LB@Position Seed - mRNA 78 -- 84@LB@Position Seed - sRNA 167 -- 173@LB@
16	0.0008966	0.1886	b0807	rlmF	-16.29	0.014793	117 -- 160	51 -- 97	23S rRNA m(6)A1618 methyltransferase SAM-dependent		_____________116_____________________________________________161\n_____________\|_______________________________________________\|\n5'-AUU...UUAAG___U_____AA______A_______________U__A_C________UUAUG...UCC-3'\n______________GUU_UGGGG____UGAU_AACGCAGCCAG_AUA_UU_C_CUGUCGCG\n______________\|\|\|_\|\|\|\|:____\|:\|:_\|\|\|\|\|\|:\|\|\|\|_\|:\|_:\|_\|_\|\|\|\|\|\|\|\|\n______________CAA_ACCCU____AUUG_UUGCGUUGGUC_UGU_GA_G_GACAGCGC\n3'-UUU...AUUAG___C_____GCGA____G___________G___U__A_C________CAUUU...UAG-5'\n_____________\|_______________________________________________\|\n_____________98______________________________________________50\n	154 -- 160	51 -- 57	-44.7843 kcal/mol	10.0752 kcal/mol	25.3667 kcal/mol	116 161@LB@ \| \|@LB@5'-AUU...UUAAG U AA A U A C UUAUG...UCC-3'@LB@ GUU UGGGG UGAU AACGCAGCCAG AUA UU C CUGUCGCG@LB@ \|\|\| \|\|\|\|: \|:\|: \|\|\|\|\|\|:\|\|\|\| \|:\| :\| \| \|\|\|\|\|\|\|\|@LB@ CAA ACCCU AUUG UUGCGUUGGUC UGU GA G GACAGCGC@LB@3'-UUU...AUUAG C GCGA G G U A C CAUUU...UAG-5'@LB@ \| \|@LB@ 98 50@LB@@LB@@LB@Energy -16.29 kcal/mol@LB@Hybridization Energy -44.7843 kcal/mol@LB@Unfolding Energy - mRNA 10.0752 kcal/mol@LB@Unfolding Energy - sRNA 25.3667 kcal/mol@LB@@LB@Position - mRNA 117 -- 160@LB@Position - sRNA 51 -- 97@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 51 -- 57@LB@
17	0.001014	0.2007	b4032	malG	-14.09	0.041561	127 -- 139	193 -- 205	maltose transporter subunit		_____________126___________140\n_____________\|_____________\|\n5'-CGU...UCUUC_____________CGCUG...UGG-3'\n______________CUGCUGGUGGGUG\n______________\|\|\|\|\|\|\|:\|\|\|\|\|\n______________GACGACCGCCCAC\n3'-UUU...UAUUA_____________UAAAA...UAG-5'\n_____________\|_____________\|\n_____________206___________192\n	132 -- 138	194 -- 200	-26.9183 kcal/mol	7.69341 kcal/mol	9.06058 kcal/mol	126 140@LB@ \| \|@LB@5'-CGU...UCUUC CGCUG...UGG-3'@LB@ CUGCUGGUGGGUG@LB@ \|\|\|\|\|\|\|:\|\|\|\|\|@LB@ GACGACCGCCCAC@LB@3'-UUU...UAUUA UAAAA...UAG-5'@LB@ \| \|@LB@ 206 192@LB@@LB@@LB@Energy -14.09 kcal/mol@LB@Hybridization Energy -26.9183 kcal/mol@LB@Unfolding Energy - mRNA 7.69341 kcal/mol@LB@Unfolding Energy - sRNA 9.06058 kcal/mol@LB@@LB@Position - mRNA 127 -- 139@LB@Position - sRNA 193 -- 205@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 194 -- 200@LB@
18	0.001156	0.216	b3474	yhhT	-13.05	0.067927	149 -- 189	169 -- 204	UPF0118 family putative transporter		_____________148__________________________________________________________________190\n_____________\|____________________________________________________________________\|\n5'-GAC...GACGU____________UAAG____AGAAUAU________U_ACUCCCGC_____U______AAAA_______AGGAA...UCA-3'\n______________GAAAAUCAGCAG____AAUC_______UGCUGGCG_G________UACAC_UAAUAC____AGUCACA\n______________:\|\|\|\|:\|\|\|\|\|\|____\|\|\|\|_______\|\|\|\|\|\|\|\|_\|________\|\|\|\|\|_:\|\|\|\|\|____\|\|\|\|\|\|\|\n______________UUUUUGGUCGUC____UUAG_______ACGACCGC_C________AUGUG_GUUAUG____UCAGUGU\n________3'-UUU____________CAUA_____________________CACUAAA_____________AG_________GUACU...UAG-5'\n_____________\|____________________________________________________________________\|\n_____________205__________________________________________________________________168\n	183 -- 189	169 -- 175	-48.4478 kcal/mol	16.2259 kcal/mol	18.3074 kcal/mol	148 190@LB@ \| \|@LB@5'-GAC...GACGU UAAG AGAAUAU U ACUCCCGC U AAAA AGGAA...UCA-3'@LB@ GAAAAUCAGCAG AAUC UGCUGGCG G UACAC UAAUAC AGUCACA@LB@ :\|\|\|\|:\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \| \|\|\|\|\| :\|\|\|\|\| \|\|\|\|\|\|\|@LB@ UUUUUGGUCGUC UUAG ACGACCGC C AUGUG GUUAUG UCAGUGU@LB@ 3'-UUU CAUA CACUAAA AG GUACU...UAG-5'@LB@ \| \|@LB@ 205 168@LB@@LB@@LB@Energy -13.05 kcal/mol@LB@Hybridization Energy -48.4478 kcal/mol@LB@Unfolding Energy - mRNA 16.2259 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 149 -- 189@LB@Position - sRNA 169 -- 204@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 169 -- 175@LB@
19	0.001698	0.2633	b2575	trmN	-12.50	0.088044	81 -- 92	14 -- 25	tRNA1(Val) (adenine(37)-N6)-methyltransferase		_____________80___________93\n_____________\|____________\|\n5'-ACC...UACAU__G_________GUAAU...GUA-3'\n______________CC_UGCUUCGUC\n______________\|\|_\|\|\|\|\|\|:\|\|\n______________GG_ACGAAGUAG\n3'-UUU...GUGGG__A_________-5'\n_____________\|___________\|\n_____________26__________14\n	222 -- 228	1 -- 7	-15.2724 kcal/mol	3.783 kcal/mol	1.04836 kcal/mol	80 93@LB@ \| \|@LB@5'-ACC...UACAU G GUAAU...GUA-3'@LB@ CC UGCUUCGUC@LB@ \|\| \|\|\|\|\|\|:\|\|@LB@ GG ACGAAGUAG@LB@3'-UUU...GUGGG A -5'@LB@ \| \|@LB@ 26 14@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -15.2724 kcal/mol@LB@Unfolding Energy - mRNA 3.783 kcal/mol@LB@Unfolding Energy - sRNA 1.04836 kcal/mol@LB@@LB@Position - mRNA 81 -- 92@LB@Position - sRNA 14 -- 25@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 1 -- 7@LB@
20	0.001715	0.2633	b3453	ugpB	-12.22	0.100645	241 -- 257	18 -- 32	sn-glycerol-3-phosphate ABC transporter periplasmic binding protein		_____________240______________258\n_____________\|________________\|\n5'-UUC...AAUUC_____U__________AAGAG...UGG-3'\n______________GCGCA_UCGGUACAAC\n______________:\|\|\|\|_:\|:\|:\|\|\|\|\|\n______________UGCGU_GGUCGUGUUG\n3'-UUU...UUGGU_____U__________AAGCG...UAG-5'\n_____________\|________________\|\n_____________33_______________17\n	180 -- 186	63 -- 69	-22.0099 kcal/mol	5.49331 kcal/mol	9.84719 kcal/mol	240 258@LB@ \| \|@LB@5'-UUC...AAUUC U AAGAG...UGG-3'@LB@ GCGCA UCGGUACAAC@LB@ :\|\|\|\| :\|:\|:\|\|\|\|\|@LB@ UGCGU GGUCGUGUUG@LB@3'-UUU...UUGGU U AAGCG...UAG-5'@LB@ \| \|@LB@ 33 17@LB@@LB@@LB@Energy -12.22 kcal/mol@LB@Hybridization Energy -22.0099 kcal/mol@LB@Unfolding Energy - mRNA 5.49331 kcal/mol@LB@Unfolding Energy - sRNA 9.84719 kcal/mol@LB@@LB@Position - mRNA 241 -- 257@LB@Position - sRNA 18 -- 32@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 63 -- 69@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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