Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
101	0.01097	0.3575	b1882	cheY	-8.64	0.475467	226 -- 255	72 -- 99	chemotaxis regulator transmitting signal to flagellar motor component	_____________225_______________256\n_____________\|_________________\|\n5'-GCG...AGGCG_______GGA_______GCAAA...UUG-3'\n______________GGGGCAU___CCCUUGC\n______________\|\|\|\|\|\|:___\|\|\|\|\|\|\|\n______________CCCCGUG___GGGAACG\n3'-UUU...GCGUA_______G_________AAGUAG-5'\n_____________\|_________________\|\n_____________100_______________71\n	38 -- 44	7 -- 13	-25.3164 kcal/mol	8.48869 kcal/mol	10.1447 kcal/mol	225 256@LB@ \| \|@LB@5'-GCG...AGGCG GGA GCAAA...UUG-3'@LB@ GGGGCAU CCCUUGC@LB@ \|\|\|\|\|\|: \|\|\|\|\|\|\|@LB@ CCCCGUG GGGAACG@LB@3'-UUU...GCGUA G AAGUAG-5'@LB@ \| \|@LB@ 100 71@LB@@LB@@LB@Energy -8.64 kcal/mol@LB@Hybridization Energy -25.3164 kcal/mol@LB@Unfolding Energy - mRNA 8.48869 kcal/mol@LB@Unfolding Energy - sRNA 10.1447 kcal/mol@LB@@LB@Position - mRNA 226 -- 255@LB@Position - sRNA 72 -- 99@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 7 -- 13@LB@
102	0.01106	0.3575	b0647	ybeT	-15.01	0.026876	192 -- 264	115 -- 176	Sel1 family TPR-like repeat protein	_____________191___________265\n_____________\|_____________\|\n5'-AUA...CGAAG__U__________AUAAA...UGG-3'\n______________CA_UCACACAUGA\n______________\|\|_\|\|\|\|\|\|\|\|\|\|\n______________GU_AGUGUGUACU\n3'-UUU...UAUGA__C__________ACGUC...UAG-5'\n_____________\|_____________\|\n_____________177___________114\n	198 -- 204	164 -- 170	-15.9732 kcal/mol	2.87622 kcal/mol	4.17773 kcal/mol	191 265@LB@ \| \|@LB@5'-AUA...CGAAG U AUAAA...UGG-3'@LB@ CA UCACACAUGA@LB@ \|\| \|\|\|\|\|\|\|\|\|\|@LB@ GU AGUGUGUACU@LB@3'-UUU...UAUGA C ACGUC...UAG-5'@LB@ \| \|@LB@ 177 114@LB@@LB@@LB@Energy -15.01 kcal/mol@LB@Hybridization Energy -15.9732 kcal/mol@LB@Unfolding Energy - mRNA 2.87622 kcal/mol@LB@Unfolding Energy - sRNA 4.17773 kcal/mol@LB@@LB@Position - mRNA 192 -- 264@LB@Position - sRNA 115 -- 176@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 164 -- 170@LB@
103	0.0111	0.3575	b2821	ptrA	-11.45	0.143539	261 -- 295	141 -- 175	protease III	_____________260_______________________________________296\n_____________\|_________________________________________\|\n5'-GCU...GCACC___A_____G______AA____AU_______CGA_______AACCA-3'\n______________CUU_AGUCA_GCA_GA__CGGG__GG_CAGC___UUCAGGA\n______________\|\|:_\|\|\|\|\|_:\|\|_\|\|__\|:\|\|__:\|_\|\|:\|___\|\|\|\|\|:\|\n______________GAG_UCAGU_UGU_CU__GUCC__UC_GUUG___AAGUCUU\n3'-UUU...GUUAU_________G___A__AC____GU__A____A_________AACGC...UAG-5'\n_____________\|_________________________________________\|\n_____________176_______________________________________140\n	289 -- 295	141 -- 147	-30.7779 kcal/mol	8.8583 kcal/mol	8.83168 kcal/mol	260 296@LB@ \| \|@LB@5'-GCU...GCACC A G AA AU CGA AACCA-3'@LB@ CUU AGUCA GCA GA CGGG GG CAGC UUCAGGA@LB@ \|\|: \|\|\|\|\| :\|\| \|\| \|:\|\| :\| \|\|:\| \|\|\|\|\|:\|@LB@ GAG UCAGU UGU CU GUCC UC GUUG AAGUCUU@LB@3'-UUU...GUUAU G A AC GU A A AACGC...UAG-5'@LB@ \| \|@LB@ 176 140@LB@@LB@@LB@Energy -11.45 kcal/mol@LB@Hybridization Energy -30.7779 kcal/mol@LB@Unfolding Energy - mRNA 8.8583 kcal/mol@LB@Unfolding Energy - sRNA 8.83168 kcal/mol@LB@@LB@Position - mRNA 261 -- 295@LB@Position - sRNA 141 -- 175@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 141 -- 147@LB@
104	0.01113	0.3575	b2108	yehA	-14.54	0.033608	284 -- 300	56 -- 72	putative fimbrial-like adhesin protein	_____________283_____________300\n_____________\|_______________\|\n5'-GUU...CUGUU___________G____-3'\n______________CAGCACAACUU_GUUG\n______________\|\|\|\|\|\|\|\|\|\|\|_\|:\|\|\n______________GUCGUGUUGAA_CGAC\n3'-UUU...CGUUG___________G____AGCGC...UAG-5'\n_____________\|________________\|\n_____________73_______________55\n	289 -- 295	61 -- 67	-24.1215 kcal/mol	2.36332 kcal/mol	11.1531 kcal/mol	283 300@LB@ \| \|@LB@5'-GUU...CUGUU G -3'@LB@ CAGCACAACUU GUUG@LB@ \|\|\|\|\|\|\|\|\|\|\| \|:\|\|@LB@ GUCGUGUUGAA CGAC@LB@3'-UUU...CGUUG G AGCGC...UAG-5'@LB@ \| \|@LB@ 73 55@LB@@LB@@LB@Energy -14.54 kcal/mol@LB@Hybridization Energy -24.1215 kcal/mol@LB@Unfolding Energy - mRNA 2.36332 kcal/mol@LB@Unfolding Energy - sRNA 11.1531 kcal/mol@LB@@LB@Position - mRNA 284 -- 300@LB@Position - sRNA 56 -- 72@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 61 -- 67@LB@
105	0.01118	0.3575	b0439	lon	-14.09	0.041610	272 -- 293	1 -- 24	DNA-binding ATP-dependent protease La	_____________271_______294\n_____________\|_________\|\n5'-AUC...CACAU_________CUUAU...GGG-3'\n______________GGUCAUCCC\n______________\|\|\|\|\|\|\|\|\|\n______________CCAGUAGGG\n3'-UUU...UAACG_________UGACG...UAG-5'\n_____________\|_________\|\n_____________25________0\n	277 -- 283	128 -- 134	-16.7868 kcal/mol	1.03204 kcal/mol	4.55487 kcal/mol	271 294@LB@ \| \|@LB@5'-AUC...CACAU CUUAU...GGG-3'@LB@ GGUCAUCCC@LB@ \|\|\|\|\|\|\|\|\|@LB@ CCAGUAGGG@LB@3'-UUU...UAACG UGACG...UAG-5'@LB@ \| \|@LB@ 25 0@LB@@LB@@LB@Energy -14.09 kcal/mol@LB@Hybridization Energy -16.7868 kcal/mol@LB@Unfolding Energy - mRNA 1.03204 kcal/mol@LB@Unfolding Energy - sRNA 4.55487 kcal/mol@LB@@LB@Position - mRNA 272 -- 293@LB@Position - sRNA 1 -- 24@LB@Position Seed - mRNA 277 -- 283@LB@Position Seed - sRNA 128 -- 134@LB@
106	0.01145	0.3575	b2350	gtrA	-11.86	0.119007	4 -- 25	148 -- 167	CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase/flippase	_____________3_____________26\n_____________\|_____________\|\n5'-GAA...UGUAU__AU_________ACAAACC-3'\n______________CU__GUCGCGCAU\n______________\|\|__\|\|\|:\|:\|\|\|\n______________GA__CAGUGUGUA\n3'-UUU...GUUAU__GU_________CUACG...UAG-5'\n_____________\|_____________\|\n_____________168___________147\n	287 -- 293	166 -- 172	-13.6011 kcal/mol	2.0532 kcal/mol	0.604639 kcal/mol	3 26@LB@ \| \|@LB@5'-GAA...UGUAU AU ACAAACC-3'@LB@ CU GUCGCGCAU@LB@ \|\| \|\|\|:\|:\|\|\|@LB@ GA CAGUGUGUA@LB@3'-UUU...GUUAU GU CUACG...UAG-5'@LB@ \| \|@LB@ 168 147@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -13.6011 kcal/mol@LB@Unfolding Energy - mRNA 2.0532 kcal/mol@LB@Unfolding Energy - sRNA 0.604639 kcal/mol@LB@@LB@Position - mRNA 4 -- 25@LB@Position - sRNA 148 -- 167@LB@Position Seed - mRNA 287 -- 293@LB@Position Seed - sRNA 166 -- 172@LB@
107	0.01169	0.3575	b3858	yihD	-12.50	0.087903	89 -- 104	63 -- 77	DUF1040 protein YihD	_____________88______________________________105\n_____________\|_______________________________\|\n5'-AUA...UUUAC___CU___________GCC____AUC_____CAUCA...CGA-3'\n______________CGC__GCCAGCACAAC___UGUU___GUCGU\n______________\|\|\|__:\|\|\|\|\|\|\|\|\|\|___:\|:\|___\|\|\|\|:\n______________GCG__UGGUCGUGUUG___GCGA___CAGCG\n3'-UUU...UGGUU___U____________AA_____________CCAUU...UAG-5'\n_____________\|_______________________________\|\n_____________78______________________________62\n	98 -- 104	63 -- 69	-31.4348 kcal/mol	4.67185 kcal/mol	17.7932 kcal/mol	88 105@LB@ \| \|@LB@5'-AUA...UUUAC CU GCC AUC CAUCA...CGA-3'@LB@ CGC GCCAGCACAAC UGUU GUCGU@LB@ \|\|\| :\|\|\|\|\|\|\|\|\|\| :\|:\| \|\|\|\|:@LB@ GCG UGGUCGUGUUG GCGA CAGCG@LB@3'-UUU...UGGUU U AA CCAUU...UAG-5'@LB@ \| \|@LB@ 78 62@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -31.4348 kcal/mol@LB@Unfolding Energy - mRNA 4.67185 kcal/mol@LB@Unfolding Energy - sRNA 17.7932 kcal/mol@LB@@LB@Position - mRNA 89 -- 104@LB@Position - sRNA 63 -- 77@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 63 -- 69@LB@
108	0.01175	0.3575	b0723	sdhA	-10.39	0.232370	178 -- 209	28 -- 62	succinate dehydrogenase flavoprotein subunit	_____________177_______________________210\n_____________\|_________________________\|\n5'-GGU...GAAAU__________G_G_AUU________UUGUG...GUG-3'\n______________UGC_CAGUCA_A_A___UGAUGCAG\n______________\|:\|_\|\|\|\|\|\|_\|_\|___\|\|\|\|\|\|\|\|\n______________AUG_GUCAGU_U_U___ACUACGUC\n3'-UUU...UGGUU___A______G_G____________CGUUC...UAG-5'\n_____________\|_________________________\|\n_____________63________________________27\n	225 -- 231	159 -- 165	-22.5908 kcal/mol	8.24888 kcal/mol	4.81331 kcal/mol	177 210@LB@ \| \|@LB@5'-GGU...GAAAU G G AUU UUGUG...GUG-3'@LB@ UGC CAGUCA A A UGAUGCAG@LB@ \|:\| \|\|\|\|\|\| \| \| \|\|\|\|\|\|\|\|@LB@ AUG GUCAGU U U ACUACGUC@LB@3'-UUU...UGGUU A G G CGUUC...UAG-5'@LB@ \| \|@LB@ 63 27@LB@@LB@@LB@Energy -10.39 kcal/mol@LB@Hybridization Energy -22.5908 kcal/mol@LB@Unfolding Energy - mRNA 8.24888 kcal/mol@LB@Unfolding Energy - sRNA 4.81331 kcal/mol@LB@@LB@Position - mRNA 178 -- 209@LB@Position - sRNA 28 -- 62@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 159 -- 165@LB@
109	0.01201	0.3575	b1096	pabC	-8.08	0.578936	115 -- 139	144 -- 163	4-amino-4-deoxychorismate lyase component of para-aminobenzoate synthase multienzyme complex	_____________114____________140\n_____________\|______________\|\n5'-GUU...GUGUA_______CG_____GGUGG...ACG-3'\n______________ACUCCUU__GCUUC\n______________\|\|:\|\|:\|__\|\|\|\|\|\n______________UGGGGGA__CGAAG\n3'-UUU...CCCCG_______A______UAG-5'\n_____________\|______________\|\n_____________164____________143\n	29 -- 35	10 -- 16	-15.037 kcal/mol	0.971533 kcal/mol	6.96912 kcal/mol	114 140@LB@ \| \|@LB@5'-GUU...GUGUA CG GGUGG...ACG-3'@LB@ ACUCCUU GCUUC@LB@ \|\|:\|\|:\| \|\|\|\|\|@LB@ UGGGGGA CGAAG@LB@3'-UUU...CCCCG A UAG-5'@LB@ \| \|@LB@ 164 143@LB@@LB@@LB@Energy -8.08 kcal/mol@LB@Hybridization Energy -15.037 kcal/mol@LB@Unfolding Energy - mRNA 0.971533 kcal/mol@LB@Unfolding Energy - sRNA 6.96912 kcal/mol@LB@@LB@Position - mRNA 115 -- 139@LB@Position - sRNA 144 -- 163@LB@Position Seed - mRNA 29 -- 35@LB@Position Seed - sRNA 10 -- 16@LB@
110	0.01217	0.3575	b1324	tpx	-10.54	0.217228	251 -- 258	126 -- 133	lipid hydroperoxide peroxidase	_____________250____________259\n_____________\|______________\|\n5'-GCG...ACUCC______________GUCUG...AAG-3'\n______________UUGUUGUCGCGGUG\n______________\|:\|:\|\|\|\|\|\|\|\|\|:\n______________AGCGACAGCGCCAU\n3'-UUU...GUUGA______________UUUAU...UAG-5'\n_____________\|______________\|\n_____________134____________125\n	99 -- 105	48 -- 54	-22.761 kcal/mol	7.03952 kcal/mol	5.88061 kcal/mol	250 259@LB@ \| \|@LB@5'-GCG...ACUCC GUCUG...AAG-3'@LB@ UUGUUGUCGCGGUG@LB@ \|:\|:\|\|\|\|\|\|\|\|\|:@LB@ AGCGACAGCGCCAU@LB@3'-UUU...GUUGA UUUAU...UAG-5'@LB@ \| \|@LB@ 134 125@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -22.761 kcal/mol@LB@Unfolding Energy - mRNA 7.03952 kcal/mol@LB@Unfolding Energy - sRNA 5.88061 kcal/mol@LB@@LB@Position - mRNA 251 -- 258@LB@Position - sRNA 126 -- 133@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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