Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
141	0.01524	0.3575	b0133	panC	-11.57	0.136229	4 -- 24	142 -- 162	pantothenate synthetase	_____________3_________25\n_____________\|_________\|\n5'-GGC...ACCGG_________UCCUA...UGC-3'\n______________CGGUCACAU\n______________\|:\|\|\|\|\|\|:\n______________GUCAGUGUG\n3'-UUU...UAUGA_________UACUA...UAG-5'\n_____________\|_________\|\n_____________163_______141\n	65 -- 71	168 -- 174	-12.1402 kcal/mol	2.67616 kcal/mol	0.276974 kcal/mol	3 25@LB@ \| \|@LB@5'-GGC...ACCGG UCCUA...UGC-3'@LB@ CGGUCACAU@LB@ \|:\|\|\|\|\|\|:@LB@ GUCAGUGUG@LB@3'-UUU...UAUGA UACUA...UAG-5'@LB@ \| \|@LB@ 163 141@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -12.1402 kcal/mol@LB@Unfolding Energy - mRNA 2.67616 kcal/mol@LB@Unfolding Energy - sRNA 0.276974 kcal/mol@LB@@LB@Position - mRNA 4 -- 24@LB@Position - sRNA 142 -- 162@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 168 -- 174@LB@
142	0.01524	0.3575	b0611	rna	-12.80	0.076457	138 -- 176	173 -- 202	ribonuclease I	_____________137_____________________________________177\n_____________\|_______________________________________\|\n5'-UAC...CCCAC________CUUUUUUA______A___GA___________GUAUG...AUU-3'\n______________CUGGCGGG________UGAUUU_AUA__UAGUAUUCAGU\n______________\|\|\|\|\|\|\|\|________\|\|\|\|\|\|_\|:\|__:\|:\|\|:\|\|\|\|\|\n______________GACCGCCC________ACUAAA_UGU__GUUAUGAGUCA\n3'-UUU...UAGAC______________________A___G____________GUGUG...UAG-5'\n_____________\|_______________________________________\|\n_____________203_____________________________________172\n	170 -- 176	173 -- 179	-31.7889 kcal/mol	9.30484 kcal/mol	14.6886 kcal/mol	137 177@LB@ \| \|@LB@5'-UAC...CCCAC CUUUUUUA A GA GUAUG...AUU-3'@LB@ CUGGCGGG UGAUUU AUA UAGUAUUCAGU@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\| \|:\| :\|:\|\|:\|\|\|\|\|@LB@ GACCGCCC ACUAAA UGU GUUAUGAGUCA@LB@3'-UUU...UAGAC A G GUGUG...UAG-5'@LB@ \| \|@LB@ 203 172@LB@@LB@@LB@Energy -12.80 kcal/mol@LB@Hybridization Energy -31.7889 kcal/mol@LB@Unfolding Energy - mRNA 9.30484 kcal/mol@LB@Unfolding Energy - sRNA 14.6886 kcal/mol@LB@@LB@Position - mRNA 138 -- 176@LB@Position - sRNA 173 -- 202@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 173 -- 179@LB@
143	0.01531	0.3575	b1641	slyB	-10.83	0.190300	158 -- 167	83 -- 92	outer membrane lipoprotein	_____________157____________________________168\n_____________\|______________________________\|\n5'-CCG...UCACA_______U______UGAC____AGG_____UUGCG...AAG-3'\n______________UAUGCCA_AUACUU____CAUG___GAUGC\n______________\|\|::\|\|\|_\|\|\|\|\|:____\|\|::___\|\|\|\|\|\n______________AUGUGGU_UAUGAG____GUGU___CUACG\n3'-UUU...CUAAA______________UCA_____GUA_____UCCGU...UAG-5'\n_____________\|______________________________\|\n_____________93_____________________________82\n	135 -- 141	182 -- 188	-20.8318 kcal/mol	7.22081 kcal/mol	7.72227 kcal/mol	157 168@LB@ \| \|@LB@5'-CCG...UCACA U UGAC AGG UUGCG...AAG-3'@LB@ UAUGCCA AUACUU CAUG GAUGC@LB@ \|\|::\|\|\| \|\|\|\|\|: \|\|:: \|\|\|\|\|@LB@ AUGUGGU UAUGAG GUGU CUACG@LB@3'-UUU...CUAAA UCA GUA UCCGU...UAG-5'@LB@ \| \|@LB@ 93 82@LB@@LB@@LB@Energy -10.83 kcal/mol@LB@Hybridization Energy -20.8318 kcal/mol@LB@Unfolding Energy - mRNA 7.22081 kcal/mol@LB@Unfolding Energy - sRNA 7.72227 kcal/mol@LB@@LB@Position - mRNA 158 -- 167@LB@Position - sRNA 83 -- 92@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 182 -- 188@LB@
144	0.01541	0.3575	b4555	yicS	-14.57	0.033136	213 -- 290	115 -- 187	putative periplasmic protein	_____________212_________________________________________________________________291\n_____________\|___________________________________________________________________\|\n5'-AUU...CAACG______C______UUU____UUUUUUC__U_____C______UCGUAUACGCCG_____C_______CUCAC...AGA-3'\n______________ACACUA_UGCUUA___UCAC_______GC___AUG_CAGGCA____________AGUCG_CUUUUAG\n______________\|\|\|\|:\|_\|:\|\|:\|___\|\|\|\|_______:\|___\|\|\|_\|\|\|\|\|\|____________\|\|\|\|:_\|\|\|\|:\|\|\n______________UGUGGU_AUGAGU___AGUG_______UG___UAC_GUCCGU____________UCAGU_GAAAGUC\n3'-UUU...UAAAA______U______C_______________UAC___________________________U_______UUAAC...UAG-5'\n_____________\|___________________________________________________________________\|\n_____________188_________________________________________________________________114\n	271 -- 277	143 -- 149	-34.4495 kcal/mol	11.6435 kcal/mol	13.2932 kcal/mol	212 291@LB@ \| \|@LB@5'-AUU...CAACG C UUU UUUUUUC U C UCGUAUACGCCG C CUCAC...AGA-3'@LB@ ACACUA UGCUUA UCAC GC AUG CAGGCA AGUCG CUUUUAG@LB@ \|\|\|\|:\| \|:\|\|:\| \|\|\|\| :\| \|\|\| \|\|\|\|\|\| \|\|\|\|: \|\|\|\|:\|\|@LB@ UGUGGU AUGAGU AGUG UG UAC GUCCGU UCAGU GAAAGUC@LB@3'-UUU...UAAAA U C UAC U UUAAC...UAG-5'@LB@ \| \|@LB@ 188 114@LB@@LB@@LB@Energy -14.57 kcal/mol@LB@Hybridization Energy -34.4495 kcal/mol@LB@Unfolding Energy - mRNA 11.6435 kcal/mol@LB@Unfolding Energy - sRNA 13.2932 kcal/mol@LB@@LB@Position - mRNA 213 -- 290@LB@Position - sRNA 115 -- 187@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 143 -- 149@LB@
145	0.01541	0.3575	b1191	cvrA	-14.91	0.028165	61 -- 91	128 -- 160	putative cation/proton antiporter	_____________60___________________________________92\n_____________\|____________________________________\|\n5'-UGA...CGGGA_____CUC_______GA___________________GUUCU...CUA-3'\n______________AGGCA___AGUUGAC__UUA_____GCGGUCAUCCU\n______________\|\|\|\|\|___\|\|\|::\|\|__\|:\|_____\|\|\|\|\|\|\|\|\|\|:\n______________UCCGU___UCAGUUG__AGU_____CGCCAGUAGGG\n3'-UUU...CUACG_______________AA___CUUAA___________UGACG...UAG-5'\n_____________\|____________________________________\|\n_____________161__________________________________127\n	85 -- 91	128 -- 134	-32.547 kcal/mol	9.03914 kcal/mol	12.2557 kcal/mol	60 92@LB@ \| \|@LB@5'-UGA...CGGGA CUC GA GUUCU...CUA-3'@LB@ AGGCA AGUUGAC UUA GCGGUCAUCCU@LB@ \|\|\|\|\| \|\|\|::\|\| \|:\| \|\|\|\|\|\|\|\|\|\|:@LB@ UCCGU UCAGUUG AGU CGCCAGUAGGG@LB@3'-UUU...CUACG AA CUUAA UGACG...UAG-5'@LB@ \| \|@LB@ 161 127@LB@@LB@@LB@Energy -14.91 kcal/mol@LB@Hybridization Energy -32.547 kcal/mol@LB@Unfolding Energy - mRNA 9.03914 kcal/mol@LB@Unfolding Energy - sRNA 12.2557 kcal/mol@LB@@LB@Position - mRNA 61 -- 91@LB@Position - sRNA 128 -- 160@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 128 -- 134@LB@
146	0.01559	0.358	b4394	yjjX	-8.60	0.483218	101 -- 114	123 -- 137	non-canonical purine NTP phosphatase ITPase/XTPase	_____________100__________________________________115\n_____________\|____________________________________\|\n5'-UGC...AAUCC______A______________UC________UU___CGAGA...CAU-3'\n______________CGCUAA_AUUCAG__GC_CAU__UGCAGGCA__UCA\n______________\|:\|:\|\|_\|:\|\|\|\|__:\|_\|\|\|__\|\|\|\|\|\|\|\|__\|\|\|\n______________GUGGUU_UGAGUC__UG_GUA__ACGUCCGU__AGU\n3'-UUU...AAAAU______A______AG__U___CU________UC___UGAAA...UAG-5'\n_____________\|____________________________________\|\n_____________138__________________________________122\n	254 -- 260	156 -- 162	-32.2061 kcal/mol	8.98762 kcal/mol	12.5635 kcal/mol	100 115@LB@ \| \|@LB@5'-UGC...AAUCC A UC UU CGAGA...CAU-3'@LB@ CGCUAA AUUCAG GC CAU UGCAGGCA UCA@LB@ \|:\|:\|\| \|:\|\|\|\| :\| \|\|\| \|\|\|\|\|\|\|\| \|\|\|@LB@ GUGGUU UGAGUC UG GUA ACGUCCGU AGU@LB@3'-UUU...AAAAU A AG U CU UC UGAAA...UAG-5'@LB@ \| \|@LB@ 138 122@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -32.2061 kcal/mol@LB@Unfolding Energy - mRNA 8.98762 kcal/mol@LB@Unfolding Energy - sRNA 12.5635 kcal/mol@LB@@LB@Position - mRNA 101 -- 114@LB@Position - sRNA 123 -- 137@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 156 -- 162@LB@
147	0.01564	0.358	b3153	yhbO	-15.63	0.020103	154 -- 186	72 -- 98	stress-resistance protein	_____________153_______________________________187\n_____________\|_________________________________\|\n5'-GUU...AUCCC__A________AAUU___CUCAU__________AACGC...UUA-3'\n______________CG_UUGUGGGA____CGC_____CCAAUGCAAC\n______________\|\|_\|\|\|\|\|\|\|\|____\|\|\|_____\|\|\|\|:\|\|\|\|\|\n______________GC_AACACCCU____GCG_____GGUUGCGUUG\n3'-UUU...AAUUA__________________AAUU___________GUCGU...UAG-5'\n_____________\|_________________________________\|\n_____________99________________________________71\n	180 -- 186	72 -- 78	-31.4938 kcal/mol	8.17407 kcal/mol	13.9341 kcal/mol	153 187@LB@ \| \|@LB@5'-GUU...AUCCC A AAUU CUCAU AACGC...UUA-3'@LB@ CG UUGUGGGA CGC CCAAUGCAAC@LB@ \|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|:\|\|\|\|\|@LB@ GC AACACCCU GCG GGUUGCGUUG@LB@3'-UUU...AAUUA AAUU GUCGU...UAG-5'@LB@ \| \|@LB@ 99 71@LB@@LB@@LB@Energy -15.63 kcal/mol@LB@Hybridization Energy -31.4938 kcal/mol@LB@Unfolding Energy - mRNA 8.17407 kcal/mol@LB@Unfolding Energy - sRNA 13.9341 kcal/mol@LB@@LB@Position - mRNA 154 -- 186@LB@Position - sRNA 72 -- 98@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 72 -- 78@LB@
148	0.01584	0.3602	b1822	rlmA	-10.68	0.203557	148 -- 239	111 -- 186	23S rRNA m(1)G745 methyltransferase SAM-dependent	_____________147___________240\n_____________\|_____________\|\n5'-AAG...GCGUG_____C_______UUGCG...GGU-3'\n______________AUUCG_GUUACAC\n______________\|:\|\|:_\|\|:\|\|\|\|\n______________UGAGU_CAGUGUG\n3'-UUU...GGUUA_____________UACUA...UAG-5'\n_____________\|_____________\|\n_____________187___________110\n	163 -- 169	168 -- 174	-12.5288 kcal/mol	1.89227 kcal/mol	0.283892 kcal/mol	147 240@LB@ \| \|@LB@5'-AAG...GCGUG C UUGCG...GGU-3'@LB@ AUUCG GUUACAC@LB@ \|:\|\|: \|\|:\|\|\|\|@LB@ UGAGU CAGUGUG@LB@3'-UUU...GGUUA UACUA...UAG-5'@LB@ \| \|@LB@ 187 110@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -12.5288 kcal/mol@LB@Unfolding Energy - mRNA 1.89227 kcal/mol@LB@Unfolding Energy - sRNA 0.283892 kcal/mol@LB@@LB@Position - mRNA 148 -- 239@LB@Position - sRNA 111 -- 186@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 168 -- 174@LB@
149	0.01599	0.3612	b3236	mdh	-10.77	0.195799	223 -- 232	196 -- 205	malate dehydrogenase NAD(P)-binding	_____________222_____233\n_____________\|_______\|\n5'-GUU...AUGAA_______GUCCU...AUG-3'\n______________AGUCGCA\n______________\|\|\|\|:\|\|\n______________UCAGUGU\n3'-UUU...AUGAG_______GUACU...UAG-5'\n_____________\|_______\|\n_____________206_____195\n	206 -- 212	169 -- 175	-8.87418 kcal/mol	1.40028 kcal/mol	0.26566 kcal/mol	222 233@LB@ \| \|@LB@5'-GUU...AUGAA GUCCU...AUG-3'@LB@ AGUCGCA@LB@ \|\|\|\|:\|\|@LB@ UCAGUGU@LB@3'-UUU...AUGAG GUACU...UAG-5'@LB@ \| \|@LB@ 206 195@LB@@LB@@LB@Energy -10.77 kcal/mol@LB@Hybridization Energy -8.87418 kcal/mol@LB@Unfolding Energy - mRNA 1.40028 kcal/mol@LB@Unfolding Energy - sRNA 0.26566 kcal/mol@LB@@LB@Position - mRNA 223 -- 232@LB@Position - sRNA 196 -- 205@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 169 -- 175@LB@
150	0.01679	0.3749	b3550	yiaC	-14.78	0.030008	125 -- 180	123 -- 174	GNAT family putative N-acetyltransferase	_____________124_______________________________________________181\n_____________\|_________________________________________________\|\n5'-AAU...UCUGU_____CU_______G_C________U___G___GA______________GGCUG...GGC-3'\n______________UACUC__UUAUGCA_G_AUGUGGGC_GGU_AAU__UCA____UGUGGUU\n______________\|\|\|\|\|__\|:\|::\|\|_\|_\|\|\|::\|\|\|_:\|\|_\|\|:__\|\|\|____\|\|:\|\|\|:\n______________AUGAG__AGUGUGU_C_UACGUCCG_UCA_UUG__AGU____ACGCCAG\n3'-UUU...UGGUU_____UC_______A__________U___G___AA___CUUA_______UAGGG...UAG-5'\n_____________\|_________________________________________________\|\n_____________175_______________________________________________122\n	134 -- 140	167 -- 173	-39.4647 kcal/mol	14.8163 kcal/mol	12.8033 kcal/mol	124 181@LB@ \| \|@LB@5'-AAU...UCUGU CU G C U G GA GGCUG...GGC-3'@LB@ UACUC UUAUGCA G AUGUGGGC GGU AAU UCA UGUGGUU@LB@ \|\|\|\|\| \|:\|::\|\| \| \|\|\|::\|\|\| :\|\| \|\|: \|\|\| \|\|:\|\|\|:@LB@ AUGAG AGUGUGU C UACGUCCG UCA UUG AGU ACGCCAG@LB@3'-UUU...UGGUU UC A U G AA CUUA UAGGG...UAG-5'@LB@ \| \|@LB@ 175 122@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -39.4647 kcal/mol@LB@Unfolding Energy - mRNA 14.8163 kcal/mol@LB@Unfolding Energy - sRNA 12.8033 kcal/mol@LB@@LB@Position - mRNA 125 -- 180@LB@Position - sRNA 123 -- 174@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 167 -- 173@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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