Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
136	0.00981	0.2504	b4063	soxR	-14.24	0.041834	39 -- 60	1 -- 21	redox-sensitive transcriptional activator of soxS; autorepressor	_____________38_____________________61\n_____________\|______________________\|\n5'-GCA...CGACU_______U_UU______C____CAAUA...GUA-3'\n______________ACAUCAA_G__AAGCGG_UGGU\n______________\|\|\|\|\|\|\|_\|__\|\|\|\|:\|_\|\|\|:\n______________UGUAGUU_C__UUCGUC_ACCG\n3'-UUU...ACCCC_______U_UU___________-5'\n_____________\|_____________________\|\n_____________22____________________1\n	39 -- 45	15 -- 21	-21.0533 kcal/mol	5.29766 kcal/mol	1.51103 kcal/mol	38 61@LB@ \| \|@LB@5'-GCA...CGACU U UU C CAAUA...GUA-3'@LB@ ACAUCAA G AAGCGG UGGU@LB@ \|\|\|\|\|\|\| \| \|\|\|\|:\| \|\|\|:@LB@ UGUAGUU C UUCGUC ACCG@LB@3'-UUU...ACCCC U UU -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -14.24 kcal/mol@LB@Hybridization Energy -21.0533 kcal/mol@LB@Unfolding Energy - mRNA 5.29766 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 39 -- 60@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 15 -- 21@LB@
137	0.0102	0.2504	b0957	ompA	-10.25	0.250752	198 -- 232	1 -- 30	outer membrane protein A (3a;II*;G;d)	_____________197_________________________________233\n_____________\|___________________________________\|\n5'-CUU...CGCAA______AAAAA____GCUAUCGC__UU________ACUGG...AAC-3'\n______________AAAAUG_____GACA________GA__GCAGUGGC\n______________\|\|\|\|\|\|_____\|\|\|\|________:\|__\|\|\|\|\|\|\|\|\n______________UUUUAC_____CUGU________UU__CGUCACCG\n3'-UUU...AGGUG______CC_______AGUUUC____UU________-5'\n_____________\|__________________________________\|\n_____________31_________________________________1\n	226 -- 232	1 -- 7	-21.624 kcal/mol	8.85247 kcal/mol	2.51911 kcal/mol	197 233@LB@ \| \|@LB@5'-CUU...CGCAA AAAAA GCUAUCGC UU ACUGG...AAC-3'@LB@ AAAAUG GACA GA GCAGUGGC@LB@ \|\|\|\|\|\| \|\|\|\| :\| \|\|\|\|\|\|\|\|@LB@ UUUUAC CUGU UU CGUCACCG@LB@3'-UUU...AGGUG CC AGUUUC UU -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -21.624 kcal/mol@LB@Unfolding Energy - mRNA 8.85247 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 198 -- 232@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 1 -- 7@LB@
138	0.0102	0.2504	b0830	gsiB	-12.88	0.082739	282 -- 298	1 -- 16	glutathione ABC transporter periplasmic binding protein	_____________281_______________299\n_____________\|_________________\|\n5'-GGC...CUGCC_____UGU_________GG-3'\n______________AAAGA___GGUGGUGGC\n______________\|\|\|\|\|___:\|::\|\|\|\|\|\n______________UUUCU___UCGUCACCG\n3'-UUU...UGUAG_____UU__________-5'\n_____________\|________________\|\n_____________17_______________1\n	292 -- 298	1 -- 7	-17.0557 kcal/mol	2.82856 kcal/mol	1.34555 kcal/mol	281 299@LB@ \| \|@LB@5'-GGC...CUGCC UGU GG-3'@LB@ AAAGA GGUGGUGGC@LB@ \|\|\|\|\| :\|::\|\|\|\|\|@LB@ UUUCU UCGUCACCG@LB@3'-UUU...UGUAG UU -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -17.0557 kcal/mol@LB@Unfolding Energy - mRNA 2.82856 kcal/mol@LB@Unfolding Energy - sRNA 1.34555 kcal/mol@LB@@LB@Position - mRNA 282 -- 298@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 1 -- 7@LB@
139	0.01022	0.2504	b3163	nlpI	-13.31	0.067339	19 -- 42	4 -- 21	lipoprotein involved in osmotic sensitivity and filamentation	_____________18_______________________43\n_____________\|________________________\|\n5'-AGG...UCUGA___________GCGACU_______CUCAA...CAU-3'\n______________GCAUUAAAGAA______GAGCAGU\n______________:\|\|\|:\|\|\|\|\|\|______:\|\|\|\|\|\|\n______________UGUAGUUUCUU______UUCGUCA\n3'-UUU...ACCCC________________________CCG-5'\n_____________\|________________________\|\n_____________22_______________________3\n	36 -- 42	4 -- 10	-18.4935 kcal/mol	3.67449 kcal/mol	1.51 kcal/mol	18 43@LB@ \| \|@LB@5'-AGG...UCUGA GCGACU CUCAA...CAU-3'@LB@ GCAUUAAAGAA GAGCAGU@LB@ :\|\|\|:\|\|\|\|\|\| :\|\|\|\|\|\|@LB@ UGUAGUUUCUU UUCGUCA@LB@3'-UUU...ACCCC CCG-5'@LB@ \| \|@LB@ 22 3@LB@@LB@@LB@Energy -13.31 kcal/mol@LB@Hybridization Energy -18.4935 kcal/mol@LB@Unfolding Energy - mRNA 3.67449 kcal/mol@LB@Unfolding Energy - sRNA 1.51 kcal/mol@LB@@LB@Position - mRNA 19 -- 42@LB@Position - sRNA 4 -- 21@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 4 -- 10@LB@
140	0.01029	0.2504	b3247	rng	-10.20	0.255190	49 -- 63	1 -- 14	ribonuclease G	_____________48______________64\n_____________\|_______________\|\n5'-GUU...CCGUU___U_______GU__AUACG...CGC-3'\n______________AGA_AAAGCAG__GC\n______________\|\|\|_\|\|\|\|\|\|\|__\|\|\n______________UCU_UUUCGUC__CG\n3'-UUU...UAGUU___________AC__-5'\n_____________\|______________\|\n_____________15_____________1\n	53 -- 59	5 -- 11	-13.7724 kcal/mol	2.22711 kcal/mol	1.34059 kcal/mol	48 64@LB@ \| \|@LB@5'-GUU...CCGUU U GU AUACG...CGC-3'@LB@ AGA AAAGCAG GC@LB@ \|\|\| \|\|\|\|\|\|\| \|\|@LB@ UCU UUUCGUC CG@LB@3'-UUU...UAGUU AC -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -10.20 kcal/mol@LB@Hybridization Energy -13.7724 kcal/mol@LB@Unfolding Energy - mRNA 2.22711 kcal/mol@LB@Unfolding Energy - sRNA 1.34059 kcal/mol@LB@@LB@Position - mRNA 49 -- 63@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 5 -- 11@LB@
142	0.01047	0.2504	b3998	nfi	-11.68	0.142276	34 -- 51	5 -- 21	endonuclease V; deoxyinosine 3' endonuclease	_____________33_________________52\n_____________\|__________________\|\n5'-UGG...UGCCC_________A________CGACU...GAG-3'\n______________GCAUUGAAG_AGAAGUAG\n______________:\|\|\|::\|\|\|_\|:\|\|\|:\|\|\n______________UGUAGUUUC_UUUUCGUC\n3'-UUU...ACCCC__________________ACCG-5'\n_____________\|__________________\|\n_____________22_________________4\n	45 -- 51	5 -- 11	-17.0993 kcal/mol	3.91468 kcal/mol	1.5073 kcal/mol	33 52@LB@ \| \|@LB@5'-UGG...UGCCC A CGACU...GAG-3'@LB@ GCAUUGAAG AGAAGUAG@LB@ :\|\|\|::\|\|\| \|:\|\|\|:\|\|@LB@ UGUAGUUUC UUUUCGUC@LB@3'-UUU...ACCCC ACCG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -17.0993 kcal/mol@LB@Unfolding Energy - mRNA 3.91468 kcal/mol@LB@Unfolding Energy - sRNA 1.5073 kcal/mol@LB@@LB@Position - mRNA 34 -- 51@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 5 -- 11@LB@
143	0.01049	0.2504	b1004	wrbA	-9.61	0.314473	272 -- 293	1 -- 25	NAD(P)H:quinone oxidoreductase	_____________271________________________294\n_____________\|__________________________\|\n5'-UUA...GCUGA___U_________C_______A____GCUGAAG-3'\n______________GGG_GCA____AG_AAAGUGG_UGGC\n______________\|\|\|_:\|\|____\|\|_\|\|\|\|::\|_\|\|\|\|\n______________CCC_UGU____UC_UUUCGUC_ACCG\n3'-UUU...UUUUA___C___AGUU__U____________-5'\n_____________\|_________________________\|\n_____________26________________________1\n	282 -- 288	5 -- 11	-20.1219 kcal/mol	7.99134 kcal/mol	2.51748 kcal/mol	271 294@LB@ \| \|@LB@5'-UUA...GCUGA U C A GCUGAAG-3'@LB@ GGG GCA AG AAAGUGG UGGC@LB@ \|\|\| :\|\| \|\| \|\|\|\|::\| \|\|\|\|@LB@ CCC UGU UC UUUCGUC ACCG@LB@3'-UUU...UUUUA C AGUU U -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -9.61 kcal/mol@LB@Hybridization Energy -20.1219 kcal/mol@LB@Unfolding Energy - mRNA 7.99134 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 272 -- 293@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 282 -- 288@LB@Position Seed - sRNA 5 -- 11@LB@
144	0.01052	0.2504	b1066	rimJ	-12.60	0.094480	174 -- 195	1 -- 22	ribosomal-protein-S5-alanine N-acetyltransferase	_____________173_____________________196\n_____________\|_______________________\|\n5'-AUU...UUAAA_______C___________A___AUAGA...AUU-3'\n______________GACGUUA_GGGAAGG_AGU_GGU\n______________\|\|\|:\|:\|_:\|:\|\|:\|_\|\|\|_\|\|:\n______________CUGUAGU_UCUUUUC_UCA_CCG\n3'-UUU...UACCC_______U_______G_______-5'\n_____________\|______________________\|\n_____________23_____________________1\n	182 -- 188	8 -- 14	-20.3318 kcal/mol	6.19349 kcal/mol	1.54023 kcal/mol	173 196@LB@ \| \|@LB@5'-AUU...UUAAA C A AUAGA...AUU-3'@LB@ GACGUUA GGGAAGG AGU GGU@LB@ \|\|\|:\|:\| :\|:\|\|:\| \|\|\| \|\|:@LB@ CUGUAGU UCUUUUC UCA CCG@LB@3'-UUU...UACCC U G -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -12.60 kcal/mol@LB@Hybridization Energy -20.3318 kcal/mol@LB@Unfolding Energy - mRNA 6.19349 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 174 -- 195@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 8 -- 14@LB@
145	0.01066	0.2504	b2153	folE	-11.37	0.162147	44 -- 68	1 -- 21	GTP cyclohydrolase I	_____________43________________________69\n_____________\|_________________________\|\n5'-CUG...UUUUA_____UGCU__U_________C___AAUUA...ACG-3'\n______________ACAUC____GA_GAAAGGCAG_GGC\n______________\|\|\|\|\|____:\|_\|\|\|\|:\|\|\|\|_\|\|\|\n______________UGUAG____UU_CUUUUCGUC_CCG\n3'-UUU...ACCCC___________U_________A___-5'\n_____________\|________________________\|\n_____________22_______________________1\n	58 -- 64	5 -- 11	-23.5216 kcal/mol	10.6442 kcal/mol	1.51103 kcal/mol	43 69@LB@ \| \|@LB@5'-CUG...UUUUA UGCU U C AAUUA...ACG-3'@LB@ ACAUC GA GAAAGGCAG GGC@LB@ \|\|\|\|\| :\| \|\|\|\|:\|\|\|\| \|\|\|@LB@ UGUAG UU CUUUUCGUC CCG@LB@3'-UUU...ACCCC U A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -11.37 kcal/mol@LB@Hybridization Energy -23.5216 kcal/mol@LB@Unfolding Energy - mRNA 10.6442 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 44 -- 68@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 5 -- 11@LB@
146	0.01079	0.2504	b2453	eutG	-16.22	0.013596	18 -- 37	1 -- 22	ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase	_____________17_____________________38\n_____________\|______________________\|\n5'-GGC...CGCCC_____AUU______________AUUAC...GUC-3'\n______________GGCAU____GAAGGGCAG_GGC\n______________\|:\|\|\|____\|\|\|::\|\|\|\|_\|\|\|\n______________CUGUA____CUUUUCGUC_CCG\n3'-UUU...UACCC_____GUUU_________A___-5'\n_____________\|_____________________\|\n_____________23____________________1\n	28 -- 34	5 -- 11	-23.1742 kcal/mol	5.41796 kcal/mol	1.54023 kcal/mol	17 38@LB@ \| \|@LB@5'-GGC...CGCCC AUU AUUAC...GUC-3'@LB@ GGCAU GAAGGGCAG GGC@LB@ \|:\|\|\| \|\|\|::\|\|\|\| \|\|\|@LB@ CUGUA CUUUUCGUC CCG@LB@3'-UUU...UACCC GUUU A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -16.22 kcal/mol@LB@Hybridization Energy -23.1742 kcal/mol@LB@Unfolding Energy - mRNA 5.41796 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 18 -- 37@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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