| 75 |
0.005596 |
0.2441 |
bsu19669 |
yoyF |
-14.84 |
0.032440 |
230 -- 255 |
26 -- 52 |
Evidence 5: No homology to any previously reported sequences |
|
_____________229_________________________256\n_____________|___________________________|\n5'-GUA...CUGGA____GAAA_________UCAUAA____AAAAA...GUC-3'\n______________CGGA____GAUUUGAUA_______UGG\n______________||||____|||:|||||_______|||\n______________GCCU____CUAGACUAU_______ACC\n3'-UUU...AAAAA____AGAA_________CUCCCCA___CCUUG...AUA-5'\n_____________|___________________________|\n_____________53__________________________25\n |
240 -- 246 |
36 -- 42 |
-17.4265 kcal/mol |
1.6069 kcal/mol |
0.980817 kcal/mol |
229 256@LB@ | |@LB@5'-GUA...CUGGA GAAA UCAUAA AAAAA...GUC-3'@LB@ CGGA GAUUUGAUA UGG@LB@ |||| |||:||||| |||@LB@ GCCU CUAGACUAU ACC@LB@3'-UUU...AAAAA AGAA CUCCCCA CCUUG...AUA-5'@LB@ | |@LB@ 53 25@LB@@LB@@LB@Energy -14.84 kcal/mol@LB@Hybridization Energy -17.4265 kcal/mol@LB@Unfolding Energy - mRNA 1.6069 kcal/mol@LB@Unfolding Energy - sRNA 0.980817 kcal/mol@LB@@LB@Position - mRNA 230 -- 255@LB@Position - sRNA 26 -- 52@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 162 |
0.01461 |
0.3008 |
bsu28700 |
ysfE |
-18.20 |
0.006050 |
31 -- 59 |
26 -- 51 |
Evidence 5: No homology to any previously reported sequences |
bsu26940 |
_____________30____________________________60\n_____________|_____________________________|\n5'-AAG...UAGAA___CUA____AAA__CUG___________CAAUG...AAA-3'\n______________GGA___UUGA___UG___AGAGGGGUUGG\n______________|||___||||___||___|||||||||||\n______________CCU___AACU___AC___UCUCCCCAACC\n3'-UUU...AAAAG___AG_____AG___UA____________CCUUG...AUA-5'\n_____________|_____________________________|\n_____________52____________________________25\n |
53 -- 59 |
26 -- 32 |
-27.3955 kcal/mol |
8.22133 kcal/mol |
0.975822 kcal/mol |
30 60@LB@ | |@LB@5'-AAG...UAGAA CUA AAA CUG CAAUG...AAA-3'@LB@ GGA UUGA UG AGAGGGGUUGG@LB@ ||| |||| || |||||||||||@LB@ CCU AACU AC UCUCCCCAACC@LB@3'-UUU...AAAAG AG AG UA CCUUG...AUA-5'@LB@ | |@LB@ 52 25@LB@@LB@@LB@Energy -18.20 kcal/mol@LB@Hybridization Energy -27.3955 kcal/mol@LB@Unfolding Energy - mRNA 8.22133 kcal/mol@LB@Unfolding Energy - sRNA 0.975822 kcal/mol@LB@@LB@Position - mRNA 31 -- 59@LB@Position - sRNA 26 -- 51@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 26 -- 32@LB@ |
| 167 |
0.01619 |
0.3152 |
bsu31500 |
yuxK |
-13.98 |
0.047449 |
182 -- 199 |
26 -- 51 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________181__________________________200\n_____________|____________________________|\n5'-UAC...UUAAA______________________AUA___AGUGA...ACG-3'\n______________GGAUC__________GGAGGGG___UGG\n______________|||||__________:||||||___|||\n______________CCUAG__________UCUCCCC___ACC\n3'-UUU...AAAAG_____AACUAGACUA_______A_____CCUUG...AUA-5'\n_____________|____________________________|\n_____________52___________________________25\n |
187 -- 193 |
30 -- 36 |
-20.9361 kcal/mol |
5.98517 kcal/mol |
0.975822 kcal/mol |
181 200@LB@ | |@LB@5'-UAC...UUAAA AUA AGUGA...ACG-3'@LB@ GGAUC GGAGGGG UGG@LB@ ||||| :|||||| |||@LB@ CCUAG UCUCCCC ACC@LB@3'-UUU...AAAAG AACUAGACUA A CCUUG...AUA-5'@LB@ | |@LB@ 52 25@LB@@LB@@LB@Energy -13.98 kcal/mol@LB@Hybridization Energy -20.9361 kcal/mol@LB@Unfolding Energy - mRNA 5.98517 kcal/mol@LB@Unfolding Energy - sRNA 0.975822 kcal/mol@LB@@LB@Position - mRNA 182 -- 199@LB@Position - sRNA 26 -- 51@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 191 |
0.01913 |
0.3269 |
bsu37760 |
rocC |
-14.43 |
0.038902 |
267 -- 280 |
26 -- 36 |
amino acid permease RocC |
|
_____________266____________281\n_____________|______________|\n5'-CCG...CUUUG________GAU___CACUG...GCU-3'\n______________GGAGGGGU___UGG\n______________:|||||||___|||\n______________UCUCCCCA___ACC\n3'-UUU...GACUA______________CCUUG...AUA-5'\n_____________|______________|\n_____________37_____________25\n |
268 -- 274 |
29 -- 35 |
-16.8756 kcal/mol |
2.30826 kcal/mol |
0.135623 kcal/mol |
266 281@LB@ | |@LB@5'-CCG...CUUUG GAU CACUG...GCU-3'@LB@ GGAGGGGU UGG@LB@ :||||||| |||@LB@ UCUCCCCA ACC@LB@3'-UUU...GACUA CCUUG...AUA-5'@LB@ | |@LB@ 37 25@LB@@LB@@LB@Energy -14.43 kcal/mol@LB@Hybridization Energy -16.8756 kcal/mol@LB@Unfolding Energy - mRNA 2.30826 kcal/mol@LB@Unfolding Energy - sRNA 0.135623 kcal/mol@LB@@LB@Position - mRNA 267 -- 280@LB@Position - sRNA 26 -- 36@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 87 |
0.00681 |
0.2588 |
bsu29140 |
citZ |
-14.51 |
0.037644 |
213 -- 231 |
27 -- 52 |
citrate synthase 2 |
|
_____________212_________________________232\n_____________|___________________________|\n5'-GAG...CGACA__C________________________UAGCA...AUG-3'\n______________CG_GGUCUUGA________AGGGGUUG\n______________||_|:||||||________||||||||\n______________GC_CUAGAACU________UCCCCAAC\n3'-UUU...AAAAA___________AGACUAUC________CCCUU...AUA-5'\n_____________|___________________________|\n_____________53__________________________26\n |
225 -- 231 |
27 -- 33 |
-23.9033 kcal/mol |
8.41647 kcal/mol |
0.980744 kcal/mol |
212 232@LB@ | |@LB@5'-GAG...CGACA C UAGCA...AUG-3'@LB@ CG GGUCUUGA AGGGGUUG@LB@ || |:|||||| ||||||||@LB@ GC CUAGAACU UCCCCAAC@LB@3'-UUU...AAAAA AGACUAUC CCCUU...AUA-5'@LB@ | |@LB@ 53 26@LB@@LB@@LB@Energy -14.51 kcal/mol@LB@Hybridization Energy -23.9033 kcal/mol@LB@Unfolding Energy - mRNA 8.41647 kcal/mol@LB@Unfolding Energy - sRNA 0.980744 kcal/mol@LB@@LB@Position - mRNA 213 -- 231@LB@Position - sRNA 27 -- 52@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 103 |
0.008788 |
0.2778 |
bsu06070 |
ydiP |
-8.66 |
0.321247 |
183 -- 194 |
27 -- 44 |
BsuMI modification methylase subunit YdiP |
bsu20250 |
_____________182________________195\n_____________|__________________|\n5'-GGA...AAAAA__________________AUAUC...UGG-3'\n______________GAUUUGA______GGUUG\n______________|||:|||______|||||\n______________CUAGACU______CCAAC\n3'-UUU...UAGAA_______AUCUCC_____CCCUU...AUA-5'\n_____________|__________________|\n_____________45_________________26\n |
183 -- 189 |
38 -- 44 |
-12.3729 kcal/mol |
2.73997 kcal/mol |
0.971452 kcal/mol |
182 195@LB@ | |@LB@5'-GGA...AAAAA AUAUC...UGG-3'@LB@ GAUUUGA GGUUG@LB@ |||:||| |||||@LB@ CUAGACU CCAAC@LB@3'-UUU...UAGAA AUCUCC CCCUU...AUA-5'@LB@ | |@LB@ 45 26@LB@@LB@@LB@Energy -8.66 kcal/mol@LB@Hybridization Energy -12.3729 kcal/mol@LB@Unfolding Energy - mRNA 2.73997 kcal/mol@LB@Unfolding Energy - sRNA 0.971452 kcal/mol@LB@@LB@Position - mRNA 183 -- 194@LB@Position - sRNA 27 -- 44@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 123 |
0.01093 |
0.2883 |
bsu22970 |
ypbH |
-15.60 |
0.022809 |
254 -- 275 |
27 -- 45 |
adapter protein MecA |
|
_____________253____________________276\n_____________|______________________|\n5'-GAG...CUCGA________C___CC____A___ACAAA...ACU-3'\n______________UGAUUUGA_AGA__GGGG_UUG\n______________||||:|||_|||__||||_|||\n______________ACUAGACU_UCU__CCCC_AAC\n3'-UUU...CUAGA________A_____________CCCUU...AUA-5'\n_____________|______________________|\n_____________46_____________________26\n |
255 -- 261 |
38 -- 44 |
-20.0369 kcal/mol |
3.46393 kcal/mol |
0.971562 kcal/mol |
253 276@LB@ | |@LB@5'-GAG...CUCGA C CC A ACAAA...ACU-3'@LB@ UGAUUUGA AGA GGGG UUG@LB@ ||||:||| ||| |||| |||@LB@ ACUAGACU UCU CCCC AAC@LB@3'-UUU...CUAGA A CCCUU...AUA-5'@LB@ | |@LB@ 46 26@LB@@LB@@LB@Energy -15.60 kcal/mol@LB@Hybridization Energy -20.0369 kcal/mol@LB@Unfolding Energy - mRNA 3.46393 kcal/mol@LB@Unfolding Energy - sRNA 0.971562 kcal/mol@LB@@LB@Position - mRNA 254 -- 275@LB@Position - sRNA 27 -- 45@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 131 |
0.01151 |
0.2883 |
bsu03110 |
ycgH |
-12.02 |
0.104581 |
161 -- 192 |
27 -- 50 |
transporter |
|
_____________160______________________________193\n_____________|________________________________|\n5'-UUU...GGACA____ACA____AACCUUA____AA________AAUAA...UCG-3'\n______________GAUC___AUCU_______GAUA__AGGGGUUG\n______________||||___||||_______||||__||||||||\n______________CUAG___UAGA_______CUAU__UCCCCAAC\n3'-UUU...AAAGC____AAC_______________C_________CCCUU...AUA-5'\n_____________|________________________________|\n_____________51_______________________________26\n |
186 -- 192 |
27 -- 33 |
-20.341 kcal/mol |
7.34553 kcal/mol |
0.975523 kcal/mol |
160 193@LB@ | |@LB@5'-UUU...GGACA ACA AACCUUA AA AAUAA...UCG-3'@LB@ GAUC AUCU GAUA AGGGGUUG@LB@ |||| |||| |||| ||||||||@LB@ CUAG UAGA CUAU UCCCCAAC@LB@3'-UUU...AAAGC AAC C CCCUU...AUA-5'@LB@ | |@LB@ 51 26@LB@@LB@@LB@Energy -12.02 kcal/mol@LB@Hybridization Energy -20.341 kcal/mol@LB@Unfolding Energy - mRNA 7.34553 kcal/mol@LB@Unfolding Energy - sRNA 0.975523 kcal/mol@LB@@LB@Position - mRNA 161 -- 192@LB@Position - sRNA 27 -- 50@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 136 |
0.01205 |
0.2883 |
bsu35319 |
yvzG |
-14.21 |
0.042782 |
258 -- 272 |
27 -- 43 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________257_______________273\n_____________|_________________|\n5'-AAG...UUACC_________________UGUUA...AUU-3'\n______________AUC_GA_AGAGGGGUUG\n______________|||_||_||||||||||\n______________UAG_CU_UCUCCCCAAC\n3'-UUU...AGAAC___A__A__________CCCUU...AUA-5'\n_____________|_________________|\n_____________44________________26\n |
266 -- 272 |
27 -- 33 |
-20.1473 kcal/mol |
4.96591 kcal/mol |
0.966729 kcal/mol |
257 273@LB@ | |@LB@5'-AAG...UUACC UGUUA...AUU-3'@LB@ AUC GA AGAGGGGUUG@LB@ ||| || ||||||||||@LB@ UAG CU UCUCCCCAAC@LB@3'-UUU...AGAAC A A CCCUU...AUA-5'@LB@ | |@LB@ 44 26@LB@@LB@@LB@Energy -14.21 kcal/mol@LB@Hybridization Energy -20.1473 kcal/mol@LB@Unfolding Energy - mRNA 4.96591 kcal/mol@LB@Unfolding Energy - sRNA 0.966729 kcal/mol@LB@@LB@Position - mRNA 258 -- 272@LB@Position - sRNA 27 -- 43@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 142 |
0.01236 |
0.2883 |
bsu37940 |
ywdJ |
-14.78 |
0.033255 |
157 -- 193 |
27 -- 52 |
purine permease YwdJ |
|
_____________156___________________________________194\n_____________|_____________________________________|\n5'-GGA...GGAAA____GAG____U_______UUUUAAAG____A_____AGCUU...ACU-3'\n______________CGGA___UCUU_AUUUGAU________AGAG_GGUUG\n______________||||___||||_||:||||________||||_|||||\n______________GCCU___AGAA_UAGACUA________UCUC_CCAAC\n3'-UUU...AAAAA___________C___________________C_____CCCUU...AUA-5'\n_____________|_____________________________________|\n_____________53____________________________________26\n |
169 -- 175 |
37 -- 43 |
-23.5373 kcal/mol |
7.77274 kcal/mol |
0.980744 kcal/mol |
156 194@LB@ | |@LB@5'-GGA...GGAAA GAG U UUUUAAAG A AGCUU...ACU-3'@LB@ CGGA UCUU AUUUGAU AGAG GGUUG@LB@ |||| |||| ||:|||| |||| |||||@LB@ GCCU AGAA UAGACUA UCUC CCAAC@LB@3'-UUU...AAAAA C C CCCUU...AUA-5'@LB@ | |@LB@ 53 26@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -23.5373 kcal/mol@LB@Unfolding Energy - mRNA 7.77274 kcal/mol@LB@Unfolding Energy - sRNA 0.980744 kcal/mol@LB@@LB@Position - mRNA 157 -- 193@LB@Position - sRNA 27 -- 52@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 37 -- 43@LB@ |
