| 90 |
0.006982 |
0.2588 |
bsu40920 |
yyaF |
-12.87 |
0.074972 |
6 -- 31 |
25 -- 50 |
GTP-dependent nucleic acid-binding protein EngD |
|
_____________5___________________________32\n_____________|___________________________|\n______5'-CAAAU___ACAAAU_______AU__AA_____AAGUA...CGU-3'\n______________GAU______UCUGAUA__GG___UGGG\n______________|||______|||||||__||___||||\n______________CUA______AGACUAU__CC___ACCC\n3'-UUU...AAAGC___GAACU________CU__CCA____CUUGU...AUA-5'\n_____________|___________________________|\n_____________51__________________________24\n |
15 -- 21 |
36 -- 42 |
-17.5884 kcal/mol |
3.73957 kcal/mol |
0.976057 kcal/mol |
5 32@LB@ | |@LB@ 5'-CAAAU ACAAAU AU AA AAGUA...CGU-3'@LB@ GAU UCUGAUA GG UGGG@LB@ ||| ||||||| || ||||@LB@ CUA AGACUAU CC ACCC@LB@3'-UUU...AAAGC GAACU CU CCA CUUGU...AUA-5'@LB@ | |@LB@ 51 24@LB@@LB@@LB@Energy -12.87 kcal/mol@LB@Hybridization Energy -17.5884 kcal/mol@LB@Unfolding Energy - mRNA 3.73957 kcal/mol@LB@Unfolding Energy - sRNA 0.976057 kcal/mol@LB@@LB@Position - mRNA 6 -- 31@LB@Position - sRNA 25 -- 50@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 92 |
0.007313 |
0.2652 |
bsu34470 |
yveA |
-16.72 |
0.013215 |
240 -- 265 |
25 -- 47 |
aspartate-proton symporter |
|
_____________239________________________266\n_____________|__________________________|\n5'-CCU...CGCUG___________UUU__UU___A____AGCCA...UCA-3'\n______________UUUGAUCUGAU___GA__GGG_UGGG\n______________:||||||||||___||__|||_||||\n______________GAACUAGACUA___CU__CCC_ACCC\n3'-UUU...GCCUA___________U____C____A____CUUGU...AUA-5'\n_____________|__________________________|\n_____________48_________________________24\n |
244 -- 250 |
37 -- 43 |
-24.6158 kcal/mol |
6.92071 kcal/mol |
0.975871 kcal/mol |
239 266@LB@ | |@LB@5'-CCU...CGCUG UUU UU A AGCCA...UCA-3'@LB@ UUUGAUCUGAU GA GGG UGGG@LB@ :|||||||||| || ||| ||||@LB@ GAACUAGACUA CU CCC ACCC@LB@3'-UUU...GCCUA U C A CUUGU...AUA-5'@LB@ | |@LB@ 48 24@LB@@LB@@LB@Energy -16.72 kcal/mol@LB@Hybridization Energy -24.6158 kcal/mol@LB@Unfolding Energy - mRNA 6.92071 kcal/mol@LB@Unfolding Energy - sRNA 0.975871 kcal/mol@LB@@LB@Position - mRNA 240 -- 265@LB@Position - sRNA 25 -- 47@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 163 |
0.01533 |
0.3122 |
bsu19639 |
yoyE |
-15.95 |
0.019304 |
185 -- 198 |
25 -- 39 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________184_____________199\n_____________|_______________|\n5'-UCA...CGCAG__A____________ACAUG...AAU-3'\n______________GA_GGAGGGGU_GGG\n______________||_:|||||||_|||\n______________CU_UCUCCCCA_CCC\n3'-UUU...CUAGA__A________A___CUUGU...AUA-5'\n_____________|_______________|\n_____________40______________24\n |
189 -- 195 |
29 -- 35 |
-22.4813 kcal/mol |
5.74668 kcal/mol |
0.783603 kcal/mol |
184 199@LB@ | |@LB@5'-UCA...CGCAG A ACAUG...AAU-3'@LB@ GA GGAGGGGU GGG@LB@ || :||||||| |||@LB@ CU UCUCCCCA CCC@LB@3'-UUU...CUAGA A A CUUGU...AUA-5'@LB@ | |@LB@ 40 24@LB@@LB@@LB@Energy -15.95 kcal/mol@LB@Hybridization Energy -22.4813 kcal/mol@LB@Unfolding Energy - mRNA 5.74668 kcal/mol@LB@Unfolding Energy - sRNA 0.783603 kcal/mol@LB@@LB@Position - mRNA 185 -- 198@LB@Position - sRNA 25 -- 39@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 182 |
0.01724 |
0.3161 |
bsu22340 |
nth |
-14.82 |
0.032761 |
30 -- 59 |
25 -- 52 |
endonuclease III |
|
_____________29______________________________60\n_____________|_______________________________|\n5'-CAG...UUGAC______________A_____AGAAAAA____CUUAA...UAG-3'\n______________CGGAUUUUG_UUUG_AUGGA_______UGGG\n______________|||||:|||_|:||_||:||_______||||\n______________GCCUAGAAC_AGAC_UAUCU_______ACCC\n3'-UUU...AAAAA_________U__________CCCCA______CUUGU...AUA-5'\n_____________|_______________________________|\n_____________53______________________________24\n |
32 -- 38 |
44 -- 50 |
-22.2299 kcal/mol |
6.43208 kcal/mol |
0.980838 kcal/mol |
29 60@LB@ | |@LB@5'-CAG...UUGAC A AGAAAAA CUUAA...UAG-3'@LB@ CGGAUUUUG UUUG AUGGA UGGG@LB@ |||||:||| |:|| ||:|| ||||@LB@ GCCUAGAAC AGAC UAUCU ACCC@LB@3'-UUU...AAAAA U CCCCA CUUGU...AUA-5'@LB@ | |@LB@ 53 24@LB@@LB@@LB@Energy -14.82 kcal/mol@LB@Hybridization Energy -22.2299 kcal/mol@LB@Unfolding Energy - mRNA 6.43208 kcal/mol@LB@Unfolding Energy - sRNA 0.980838 kcal/mol@LB@@LB@Position - mRNA 30 -- 59@LB@Position - sRNA 25 -- 52@LB@Position Seed - mRNA 32 -- 38@LB@Position Seed - sRNA 44 -- 50@LB@ |
| 185 |
0.01858 |
0.3269 |
bsu27560 |
hisS |
-8.40 |
0.345340 |
122 -- 132 |
25 -- 38 |
histidine--tRNA ligase |
|
_____________121____________133\n_____________|______________|\n5'-GCG...GUAUA_______C______AGUUU...CUU-3'\n______________GUAGGGG____GGG\n______________:|||:||____|||\n______________UAUCUCC____CCC\n3'-UUU...UAGAC_______CCAA___CUUGU...AUA-5'\n_____________|______________|\n_____________39_____________24\n |
122 -- 128 |
32 -- 38 |
-11.8618 kcal/mol |
2.79445 kcal/mol |
0.662893 kcal/mol |
121 133@LB@ | |@LB@5'-GCG...GUAUA C AGUUU...CUU-3'@LB@ GUAGGGG GGG@LB@ :|||:|| |||@LB@ UAUCUCC CCC@LB@3'-UUU...UAGAC CCAA CUUGU...AUA-5'@LB@ | |@LB@ 39 24@LB@@LB@@LB@Energy -8.40 kcal/mol@LB@Hybridization Energy -11.8618 kcal/mol@LB@Unfolding Energy - mRNA 2.79445 kcal/mol@LB@Unfolding Energy - sRNA 0.662893 kcal/mol@LB@@LB@Position - mRNA 122 -- 132@LB@Position - sRNA 25 -- 38@LB@Position Seed - mRNA 122 -- 128@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 8 |
5.206e-05 |
0.02171 |
bsu37180 |
fadF |
-12.43 |
0.089383 |
154 -- 199 |
26 -- 74 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 17189250; Product type pe : enzyme' |
|
_____________153____________________________________________________200\n_____________|______________________________________________________|\n5'-CAA...AGAUU_________G_GA___________C______U___A_UAAAA______AA____AAUGA...ACA-3'\n______________CGGGGGUAG_G__________UCG_GGUCUU_AUC_G_____AGGGGG__UUGG\n______________|||||||||_|__________|||_|:||||_|||_|_____||:|||__||||\n______________GCCCCCAUC_C__________AGC_CUAGAA_UAG_C_____UCUCCC__AACC\n3'-UUU...GAACC_________G_GGUUCAAAAA__________C___A_UA_________C_____CCUUG...AUA-5'\n_____________|______________________________________________________|\n_____________75_____________________________________________________25\n |
156 -- 162 |
66 -- 72 |
-40.4869 kcal/mol |
12.2576 kcal/mol |
15.8011 kcal/mol |
153 200@LB@ | |@LB@5'-CAA...AGAUU G GA C U A UAAAA AA AAUGA...ACA-3'@LB@ CGGGGGUAG G UCG GGUCUU AUC G AGGGGG UUGG@LB@ ||||||||| | ||| |:|||| ||| | ||:||| ||||@LB@ GCCCCCAUC C AGC CUAGAA UAG C UCUCCC AACC@LB@3'-UUU...GAACC G GGUUCAAAAA C A UA C CCUUG...AUA-5'@LB@ | |@LB@ 75 25@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -40.4869 kcal/mol@LB@Unfolding Energy - mRNA 12.2576 kcal/mol@LB@Unfolding Energy - sRNA 15.8011 kcal/mol@LB@@LB@Position - mRNA 154 -- 199@LB@Position - sRNA 26 -- 74@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 13 |
0.0002088 |
0.05257 |
bsu22150 |
ypvA |
-19.04 |
0.003759 |
161 -- 194 |
26 -- 53 |
ATP-dependent helicase YpvA |
|
_____________160____________________________________195\n_____________|______________________________________|\n5'-GUG...UAUUA________________AUGUCGAA___UU_________AACUA...CCG-3'\n______________UUGG____UGGUCUGA________UAG__GGGGGUUGG\n______________|:||____||:|||||________|||__:||||||||\n______________AGCC____ACUAGACU________AUC__UCCCCAACC\n3'-UUU...AAAAA____UAGA______________________________CCUUG...AUA-5'\n_____________|______________________________________|\n_____________54_____________________________________25\n |
188 -- 194 |
26 -- 32 |
-27.605 kcal/mol |
7.58324 kcal/mol |
0.986187 kcal/mol |
160 195@LB@ | |@LB@5'-GUG...UAUUA AUGUCGAA UU AACUA...CCG-3'@LB@ UUGG UGGUCUGA UAG GGGGGUUGG@LB@ |:|| ||:||||| ||| :||||||||@LB@ AGCC ACUAGACU AUC UCCCCAACC@LB@3'-UUU...AAAAA UAGA CCUUG...AUA-5'@LB@ | |@LB@ 54 25@LB@@LB@@LB@Energy -19.04 kcal/mol@LB@Hybridization Energy -27.605 kcal/mol@LB@Unfolding Energy - mRNA 7.58324 kcal/mol@LB@Unfolding Energy - sRNA 0.986187 kcal/mol@LB@@LB@Position - mRNA 161 -- 194@LB@Position - sRNA 26 -- 53@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 26 -- 32@LB@ |
| 21 |
0.0003947 |
0.0627 |
bsu28260 |
leuC |
-16.01 |
0.018761 |
166 -- 191 |
26 -- 52 |
3-isopropylmalate dehydratase large subunit |
|
_____________165___________________________192\n_____________|_____________________________|\n5'-GAA...UUAAG________A_C___A_________A____AAAUA...UUC-3'\n______________CGG_UCUU_G_UCU_GGUAGAGGG___GG\n______________|||_||||_|_|||_|:|||||||___||\n______________GCC_AGAA_C_AGA_CUAUCUCCC___CC\n3'-UUU...AAAAA___U______U_____________CAA__CCUUG...AUA-5'\n_____________|_____________________________|\n_____________53____________________________25\n |
182 -- 188 |
31 -- 37 |
-26.3594 kcal/mol |
9.36849 kcal/mol |
0.980817 kcal/mol |
165 192@LB@ | |@LB@5'-GAA...UUAAG A C A A AAAUA...UUC-3'@LB@ CGG UCUU G UCU GGUAGAGGG GG@LB@ ||| |||| | ||| |:||||||| ||@LB@ GCC AGAA C AGA CUAUCUCCC CC@LB@3'-UUU...AAAAA U U CAA CCUUG...AUA-5'@LB@ | |@LB@ 53 25@LB@@LB@@LB@Energy -16.01 kcal/mol@LB@Hybridization Energy -26.3594 kcal/mol@LB@Unfolding Energy - mRNA 9.36849 kcal/mol@LB@Unfolding Energy - sRNA 0.980817 kcal/mol@LB@@LB@Position - mRNA 166 -- 191@LB@Position - sRNA 26 -- 52@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 53 |
0.002724 |
0.1697 |
bsu06970 |
yesO |
-13.13 |
0.067695 |
188 -- 199 |
26 -- 36 |
ABC transporter substrate-binding protein |
|
_____________187__________200\n_____________|____________|\n5'-CGA...AUUAA_______A____UUUGA...UUA-3'\n______________AGGGGGG_UUGG\n______________||:||||_||||\n______________UCUCCCC_AACC\n3'-UUU...GACUA____________CCUUG...AUA-5'\n_____________|____________|\n_____________37___________25\n |
188 -- 194 |
30 -- 36 |
-17.5942 kcal/mol |
4.33325 kcal/mol |
0.135623 kcal/mol |
187 200@LB@ | |@LB@5'-CGA...AUUAA A UUUGA...UUA-3'@LB@ AGGGGGG UUGG@LB@ ||:|||| ||||@LB@ UCUCCCC AACC@LB@3'-UUU...GACUA CCUUG...AUA-5'@LB@ | |@LB@ 37 25@LB@@LB@@LB@Energy -13.13 kcal/mol@LB@Hybridization Energy -17.5942 kcal/mol@LB@Unfolding Energy - mRNA 4.33325 kcal/mol@LB@Unfolding Energy - sRNA 0.135623 kcal/mol@LB@@LB@Position - mRNA 188 -- 199@LB@Position - sRNA 26 -- 36@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 61 |
0.003607 |
0.1972 |
bsu06860 |
yezE |
-12.02 |
0.104394 |
226 -- 262 |
26 -- 52 |
TetR family transcriptional regulator |
|
_____________225___________________________________263\n_____________|_____________________________________|\n5'-AGG...UGAUA___CG____U___UCACAGGGC________UUU____AGAGU...UGC-3'\n______________CGG__CUUG_UCU_________GAUAGAGG___UUGG\n______________|||__||||_|||_________||||||||___||||\n______________GCC__GAAC_AGA_________CUAUCUCC___AACC\n3'-UUU...AAAAA___UA____U____________________CC_____CCUUG...AUA-5'\n_____________|_____________________________________|\n_____________53____________________________________25\n |
249 -- 255 |
32 -- 38 |
-24.4528 kcal/mol |
11.4473 kcal/mol |
0.980817 kcal/mol |
225 263@LB@ | |@LB@5'-AGG...UGAUA CG U UCACAGGGC UUU AGAGU...UGC-3'@LB@ CGG CUUG UCU GAUAGAGG UUGG@LB@ ||| |||| ||| |||||||| ||||@LB@ GCC GAAC AGA CUAUCUCC AACC@LB@3'-UUU...AAAAA UA U CC CCUUG...AUA-5'@LB@ | |@LB@ 53 25@LB@@LB@@LB@Energy -12.02 kcal/mol@LB@Hybridization Energy -24.4528 kcal/mol@LB@Unfolding Energy - mRNA 11.4473 kcal/mol@LB@Unfolding Energy - sRNA 0.980817 kcal/mol@LB@@LB@Position - mRNA 226 -- 262@LB@Position - sRNA 26 -- 52@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 32 -- 38@LB@ |
