Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
47	0.005156	0.3601	b2614	grpE	-12.03	0.036099	152 -- 179	1 -- 26	heat shock protein		_____________151__________________________180\n_____________\|____________________________\|\n5'-GAG...CCCCA_______C________CGA_____AU__GAAAA...CUG-3'\n______________UAAUAAG_GAAGUUAG___GAUGA__GC\n______________\|\|\|\|\|\|:_:\|\|\|\|\|\|:___\|\|\|\|\|__\|\|\n______________AUUAUUU_UUUCAAUU___CUACU__CG\n3'-UUU...CUGCC_______A________A_______AU__-5'\n_____________\|___________________________\|\n_____________27__________________________1\n	161 -- 167	11 -- 17	-17.6467 kcal/mol	5.03361 kcal/mol	0.580494 kcal/mol	151 180@LB@ \| \|@LB@5'-GAG...CCCCA C CGA AU GAAAA...CUG-3'@LB@ UAAUAAG GAAGUUAG GAUGA GC@LB@ \|\|\|\|\|\|: :\|\|\|\|\|\|: \|\|\|\|\| \|\|@LB@ AUUAUUU UUUCAAUU CUACU CG@LB@3'-UUU...CUGCC A A AU -5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -12.03 kcal/mol@LB@Hybridization Energy -17.6467 kcal/mol@LB@Unfolding Energy - mRNA 5.03361 kcal/mol@LB@Unfolding Energy - sRNA 0.580494 kcal/mol@LB@@LB@Position - mRNA 152 -- 179@LB@Position - sRNA 1 -- 26@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 11 -- 17@LB@
27	0.001735	0.211	b3052	hldE	-15.91	0.004078	227 -- 261	1 -- 47	heptose 7-phosphate kinase and heptose 1-phosphate adenyltransferase		_____________226______________________________________________262\n_____________\|________________________________________________\|\n5'-GCG...UUUGA____G_____________________________________UGAU__UGGAU...CGC-3'\n______________ACGU_CAG____GAGUGAUGGU_________GGUUGGUGAUG____GC\n______________\|\|:\|_\|\|\|____\|\|:\|\|\|:\|\|\|_________:\|\|\|::\|\|\|\|\|____\|\|\n______________UGUA_GUC____CUUACUGCCA_________UCAAUUACUAC____CG\n3'-UUU...UUGUC____A___UUUA__________UUAUUUAUU___________UAU___-5'\n_____________\|_______________________________________________\|\n_____________48______________________________________________1\n	249 -- 255	6 -- 12	-30.7414 kcal/mol	8.50852 kcal/mol	6.32011 kcal/mol	226 262@LB@ \| \|@LB@5'-GCG...UUUGA G UGAU UGGAU...CGC-3'@LB@ ACGU CAG GAGUGAUGGU GGUUGGUGAUG GC@LB@ \|\|:\| \|\|\| \|\|:\|\|\|:\|\|\| :\|\|\|::\|\|\|\|\| \|\|@LB@ UGUA GUC CUUACUGCCA UCAAUUACUAC CG@LB@3'-UUU...UUGUC A UUUA UUAUUUAUU UAU -5'@LB@ \| \|@LB@ 48 1@LB@@LB@@LB@Energy -15.91 kcal/mol@LB@Hybridization Energy -30.7414 kcal/mol@LB@Unfolding Energy - mRNA 8.50852 kcal/mol@LB@Unfolding Energy - sRNA 6.32011 kcal/mol@LB@@LB@Position - mRNA 227 -- 261@LB@Position - sRNA 1 -- 47@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 6 -- 12@LB@
91	0.01489	0.5059	b3805	hemC	-12.98	0.021909	168 -- 195	24 -- 52	hydroxymethylbilane synthase		_____________167___________________________196\n_____________\|_____________________________\|\n5'-AAA...GUUAG_______CA_____UGAU___________CAAGC...GCC-3'\n______________GAUGGAC___CGGA____AAUGACGGUAA\n______________:\|::\|\|\|___\|:\|\|____\|\|\|\|\|\|\|\|\|\|\|\n______________UUGUCUG___GUCU____UUACUGCCAUU\n3'-UUU...CCCAU_______UAA____UUAC___________AUUUA...UCG-5'\n_____________\|_____________________________\|\n_____________53____________________________23\n	189 -- 195	24 -- 30	-23.6136 kcal/mol	4.33145 kcal/mol	6.2974 kcal/mol	167 196@LB@ \| \|@LB@5'-AAA...GUUAG CA UGAU CAAGC...GCC-3'@LB@ GAUGGAC CGGA AAUGACGGUAA@LB@ :\|::\|\|\| \|:\|\| \|\|\|\|\|\|\|\|\|\|\|@LB@ UUGUCUG GUCU UUACUGCCAUU@LB@3'-UUU...CCCAU UAA UUAC AUUUA...UCG-5'@LB@ \| \|@LB@ 53 23@LB@@LB@@LB@Energy -12.98 kcal/mol@LB@Hybridization Energy -23.6136 kcal/mol@LB@Unfolding Energy - mRNA 4.33145 kcal/mol@LB@Unfolding Energy - sRNA 6.2974 kcal/mol@LB@@LB@Position - mRNA 168 -- 195@LB@Position - sRNA 24 -- 52@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 24 -- 30@LB@
146	0.02455	0.526	b0491	fetB	-8.60	0.179526	114 -- 156	14 -- 57	iron export ABC transporter permease; peroxide resistance protein		_____________113_______________________________________________157\n_____________\|_________________________________________________\|\n5'-GCC...GCUGU______C___C____AAGAC_____U___CAU_________________GAUUA...AGA-3'\n______________GGGUGA_ACA_GAUA_____GAAAU_AAU___GCGG______AUAAAGU\n______________\|\|\|\|:\|_\|\|\|_\|\|:\|_____\|\|\|\|\|_\|\|\|___:\|\|\|______\|\|\|\|\|\|\|\n______________CCCAUU_UGU_CUGU_____CUUUA_UUA___UGCC______UAUUUCA\n3'-UUU...UUAUC_______________AAGU______C___C______AUUAUU_______AUUAC...UCG-5'\n_____________\|_________________________________________________\|\n_____________58________________________________________________13\n	150 -- 156	14 -- 20	-22.3302 kcal/mol	7.40722 kcal/mol	6.32606 kcal/mol	113 157@LB@ \| \|@LB@5'-GCC...GCUGU C C AAGAC U CAU GAUUA...AGA-3'@LB@ GGGUGA ACA GAUA GAAAU AAU GCGG AUAAAGU@LB@ \|\|\|\|:\| \|\|\| \|\|:\| \|\|\|\|\| \|\|\| :\|\|\| \|\|\|\|\|\|\|@LB@ CCCAUU UGU CUGU CUUUA UUA UGCC UAUUUCA@LB@3'-UUU...UUAUC AAGU C C AUUAUU AUUAC...UCG-5'@LB@ \| \|@LB@ 58 13@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -22.3302 kcal/mol@LB@Unfolding Energy - mRNA 7.40722 kcal/mol@LB@Unfolding Energy - sRNA 6.32606 kcal/mol@LB@@LB@Position - mRNA 114 -- 156@LB@Position - sRNA 14 -- 57@LB@Position Seed - mRNA 150 -- 156@LB@Position Seed - sRNA 14 -- 20@LB@
150	0.02489	0.526	b2088	insE1	-9.25	0.136086	235 -- 276	1 -- 55	IS3 transposase A	b0298 b0373 b0540 b1027	_____________234_____________________________________________________277\n_____________\|_______________________________________________________\|\n5'-ACC...ACCCC_______________CC_______UU__C____________CCC___________UUGCU...CUG-3'\n______________GUAAACAG_CAUUCG____UGAAU__CG______AGUGAAG____UGA____AGC\n______________\|\|\|\|\|\|\|\|_\|\|\|\|\|:____\|\|\|\|\|__\|\|______\|:\|:\|\|\|____\|\|\|____\|\|\|\n______________CAUUUGUC_GUAAGU____ACUUA__GC______UUAUUUC____ACU____UCG\n3'-UUU...AUCCC________U______CUUU_____CU__CAUUAU_______AAUU___ACUA___-5'\n_____________\|______________________________________________________\|\n_____________56_____________________________________________________1\n	261 -- 267	15 -- 21	-23.7484 kcal/mol	8.16492 kcal/mol	6.33758 kcal/mol	234 277@LB@ \| \|@LB@5'-ACC...ACCCC CC UU C CCC UUGCU...CUG-3'@LB@ GUAAACAG CAUUCG UGAAU CG AGUGAAG UGA AGC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|: \|\|\|\|\| \|\| \|:\|:\|\|\| \|\|\| \|\|\|@LB@ CAUUUGUC GUAAGU ACUUA GC UUAUUUC ACU UCG@LB@3'-UUU...AUCCC U CUUU CU CAUUAU AAUU ACUA -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -9.25 kcal/mol@LB@Hybridization Energy -23.7484 kcal/mol@LB@Unfolding Energy - mRNA 8.16492 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 235 -- 276@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 15 -- 21@LB@
85	0.01268	0.4896	b1827	kdgR	-11.91	0.038409	291 -- 299	56 -- 64	KDG regulon transcriptional repressor		_____________290_______300\n_____________\|_________\|\n5'-GCC...AGCGC_________A-3'\n______________GAAAUAGGG\n______________\|\|\|\|\|\|\|\|\|\n______________CUUUAUCCC\n3'-UUU...GCCAA_________CAUUU...UCG-5'\n_____________\|_________\|\n_____________65________55\n	293 -- 299	56 -- 62	-12.6296 kcal/mol	0.690209 kcal/mol	0.02823 kcal/mol	290 300@LB@ \| \|@LB@5'-GCC...AGCGC A-3'@LB@ GAAAUAGGG@LB@ \|\|\|\|\|\|\|\|\|@LB@ CUUUAUCCC@LB@3'-UUU...GCCAA CAUUU...UCG-5'@LB@ \| \|@LB@ 65 55@LB@@LB@@LB@Energy -11.91 kcal/mol@LB@Hybridization Energy -12.6296 kcal/mol@LB@Unfolding Energy - mRNA 0.690209 kcal/mol@LB@Unfolding Energy - sRNA 0.02823 kcal/mol@LB@@LB@Position - mRNA 291 -- 299@LB@Position - sRNA 56 -- 64@LB@Position Seed - mRNA 293 -- 299@LB@Position Seed - sRNA 56 -- 62@LB@
145	0.02447	0.526	b4518	ymdF	-10.96	0.061577	258 -- 296	23 -- 64	KGG family protein		_____________257________________________________________________297\n_____________\|__________________________________________________\|\n5'-UUU...CAUCA____C___UAAAAAA_________C___GC_____________G______UUCA-3'\n______________GAAG_AGG_______GGUGGACAG_ACA_____________GG_GGUAAU\n______________\|\|\|:_\|\|\|_______\|\|\|::\|\|\|\|_\|\|\|_____________\|:_\|\|\|\|\|\|\n______________CUUU_UCC_______CCAUUUGUC_UGU_____________CU_CCAUUA\n3'-UUU...GCCAA____A_______________________AAGUCUUUACUUA__G______UUUAU...UCG-5'\n_____________\|__________________________________________________\|\n_____________65_________________________________________________22\n	275 -- 281	48 -- 54	-22.543 kcal/mol	5.2615 kcal/mol	6.31898 kcal/mol	257 297@LB@ \| \|@LB@5'-UUU...CAUCA C UAAAAAA C GC G UUCA-3'@LB@ GAAG AGG GGUGGACAG ACA GG GGUAAU@LB@ \|\|\|: \|\|\| \|\|\|::\|\|\|\| \|\|\| \|: \|\|\|\|\|\|@LB@ CUUU UCC CCAUUUGUC UGU CU CCAUUA@LB@3'-UUU...GCCAA A AAGUCUUUACUUA G UUUAU...UCG-5'@LB@ \| \|@LB@ 65 22@LB@@LB@@LB@Energy -10.96 kcal/mol@LB@Hybridization Energy -22.543 kcal/mol@LB@Unfolding Energy - mRNA 5.2615 kcal/mol@LB@Unfolding Energy - sRNA 6.31898 kcal/mol@LB@@LB@Position - mRNA 258 -- 296@LB@Position - sRNA 23 -- 64@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 48 -- 54@LB@
156	0.02543	0.526	b2805	fucR	-9.08	0.146396	128 -- 135	52 -- 59	l-fucose operon activator		_____________127______136\n_____________\|________\|\n5'-CAC...AAAAA________CACCG...AAA-3'\n______________AGGGGUAA\n______________\|\|\|\|\|\|\|\|\n______________UCCCCAUU\n3'-UUU...CUUUA________UGUCU...UCG-5'\n_____________\|________\|\n_____________60_______51\n	129 -- 135	52 -- 58	-12.2757 kcal/mol	3.13783 kcal/mol	0.0599826 kcal/mol	127 136@LB@ \| \|@LB@5'-CAC...AAAAA CACCG...AAA-3'@LB@ AGGGGUAA@LB@ \|\|\|\|\|\|\|\|@LB@ UCCCCAUU@LB@3'-UUU...CUUUA UGUCU...UCG-5'@LB@ \| \|@LB@ 60 51@LB@@LB@@LB@Energy -9.08 kcal/mol@LB@Hybridization Energy -12.2757 kcal/mol@LB@Unfolding Energy - mRNA 3.13783 kcal/mol@LB@Unfolding Energy - sRNA 0.0599826 kcal/mol@LB@@LB@Position - mRNA 128 -- 135@LB@Position - sRNA 52 -- 59@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 52 -- 58@LB@
72	0.01058	0.4523	b3458	livK	-8.91	0.157650	183 -- 215	28 -- 63	leucine transporter subunit		_____________182___________________________________216\n_____________\|_____________________________________\|\n5'-UGU...AUCAC________AUUUUU____UAU______CG________AAAAC...CGA-3'\n______________GAAUGGGG_______GAC_____GAAA__GAAUG_CG\n______________:\|\|\|:\|\|\|_______\|\|\|_____\|\|\|\|__\|\|\|\|\|_\|\|\n______________UUUAUCCC_______CUG_____CUUU__CUUAC_GC\n3'-UUU...CCAAC________CAUUUGU___UAAGU____A______U__CAUUA...UCG-5'\n_____________\|_____________________________________\|\n_____________64____________________________________27\n	184 -- 190	56 -- 62	-20.644 kcal/mol	5.44449 kcal/mol	6.29422 kcal/mol	182 216@LB@ \| \|@LB@5'-UGU...AUCAC AUUUUU UAU CG AAAAC...CGA-3'@LB@ GAAUGGGG GAC GAAA GAAUG CG@LB@ :\|\|\|:\|\|\| \|\|\| \|\|\|\| \|\|\|\|\| \|\|@LB@ UUUAUCCC CUG CUUU CUUAC GC@LB@3'-UUU...CCAAC CAUUUGU UAAGU A U CAUUA...UCG-5'@LB@ \| \|@LB@ 64 27@LB@@LB@@LB@Energy -8.91 kcal/mol@LB@Hybridization Energy -20.644 kcal/mol@LB@Unfolding Energy - mRNA 5.44449 kcal/mol@LB@Unfolding Energy - sRNA 6.29422 kcal/mol@LB@@LB@Position - mRNA 183 -- 215@LB@Position - sRNA 28 -- 63@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 56 -- 62@LB@
1	2.411e-07	0.0007914	b0889	lrp	-21.69	0.000065	168 -- 212	1 -- 64	leucine-responsive global transcriptional regulator		_____________167_______________________________________________________________213\n_____________\|_________________________________________________________________\|\n5'-GUG...AGACA_________AA_______AUA_____________________________________A______AAGAA...AGC-3'\n______________GGAGUAGGG______GGA____CAGAGA_____GAC_______AAUAA___UAAUGGU_GAUAGC\n______________\|:\|:\|\|\|\|\|______:\|\|____\|\|\|\|:\|_____\|\|\|_______\|\|\|\|\|___\|\|\|\|\|:\|_\|\|\|\|\|\|\n______________CUUUAUCCC______UCU____GUCUUU_____CUG_______UUAUU___AUUACUA_CUAUCG\n3'-UUU...GCCAA_________CAUUUG___GUAA______ACUUA___CCAUUAU_____UCA______________-5'\n_____________\|________________________________________________________________\|\n_____________65_______________________________________________________________1\n	199 -- 205	7 -- 13	-30.6118 kcal/mol	2.57614 kcal/mol	6.344 kcal/mol	167 213@LB@ \| \|@LB@5'-GUG...AGACA AA AUA A AAGAA...AGC-3'@LB@ GGAGUAGGG GGA CAGAGA GAC AAUAA UAAUGGU GAUAGC@LB@ \|:\|:\|\|\|\|\| :\|\| \|\|\|\|:\| \|\|\| \|\|\|\|\| \|\|\|\|\|:\| \|\|\|\|\|\|@LB@ CUUUAUCCC UCU GUCUUU CUG UUAUU AUUACUA CUAUCG@LB@3'-UUU...GCCAA CAUUUG GUAA ACUUA CCAUUAU UCA -5'@LB@ \| \|@LB@ 65 1@LB@@LB@@LB@Energy -21.69 kcal/mol@LB@Hybridization Energy -30.6118 kcal/mol@LB@Unfolding Energy - mRNA 2.57614 kcal/mol@LB@Unfolding Energy - sRNA 6.344 kcal/mol@LB@@LB@Position - mRNA 168 -- 212@LB@Position - sRNA 1 -- 64@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 7 -- 13@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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