Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
90	0.01478	0.5059	b0740	tolB	-11.20	0.054749	186 -- 207	1 -- 21	periplasmic protein		_____________185_____________________208\n_____________\|_______________________\|\n5'-UCC...GUCCA_______GAGAU___________AGGCA...CCG-3'\n______________GAUAAGG_____AUGAUGA_AGC\n______________:\|\|\|\|:\|_____\|\|\|\|\|\|\|_\|\|\|\n______________UUAUUUC_____UACUACU_UCG\n3'-UUU...AUUAU_______AAU_________A___-5'\n_____________\|______________________\|\n_____________22_____________________1\n	198 -- 204	5 -- 11	-16.1118 kcal/mol	4.84175 kcal/mol	0.0669945 kcal/mol	185 208@LB@ \| \|@LB@5'-UCC...GUCCA GAGAU AGGCA...CCG-3'@LB@ GAUAAGG AUGAUGA AGC@LB@ :\|\|\|\|:\| \|\|\|\|\|\|\| \|\|\|@LB@ UUAUUUC UACUACU UCG@LB@3'-UUU...AUUAU AAU A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -11.20 kcal/mol@LB@Hybridization Energy -16.1118 kcal/mol@LB@Unfolding Energy - mRNA 4.84175 kcal/mol@LB@Unfolding Energy - sRNA 0.0669945 kcal/mol@LB@@LB@Position - mRNA 186 -- 207@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 5 -- 11@LB@
199	0.03375	0.5555	b3860	dsbA	-9.14	0.142422	273 -- 281	48 -- 56	periplasmic protein disulfide isomerase I		_____________272_______282\n_____________\|_________\|\n5'-UUU...AAGAU_________UACAC...AAC-3'\n______________GGUAAACAG\n______________\|\|\|\|\|\|\|\|\|\n______________CCAUUUGUC\n3'-UUU...UAUCC_________UGUAA...UCG-5'\n_____________\|_________\|\n_____________57________47\n	275 -- 281	48 -- 54	-12.2563 kcal/mol	1.07557 kcal/mol	2.03935 kcal/mol	272 282@LB@ \| \|@LB@5'-UUU...AAGAU UACAC...AAC-3'@LB@ GGUAAACAG@LB@ \|\|\|\|\|\|\|\|\|@LB@ CCAUUUGUC@LB@3'-UUU...UAUCC UGUAA...UCG-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -9.14 kcal/mol@LB@Hybridization Energy -12.2563 kcal/mol@LB@Unfolding Energy - mRNA 1.07557 kcal/mol@LB@Unfolding Energy - sRNA 2.03935 kcal/mol@LB@@LB@Position - mRNA 273 -- 281@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 48 -- 54@LB@
168	0.02737	0.531	b1385	feaB	-11.89	0.038862	180 -- 210	1 -- 28	phenylacetaldehyde dehydrogenase		_____________179_____________________________211\n_____________\|_______________________________\|\n5'-UUG...CACUG_____AU_______AAA________CAG___CGCAU...GUG-3'\n______________GGUAA__AAUAAGG___AGUGAUGA___AGC\n______________\|\|\|\|\|__\|\|\|\|\|:\|___\|:\|\|\|\|\|\|___\|\|\|\n______________CCAUU__UUAUUUC___UUACUACU___UCG\n3'-UUU...UACUG_____AU_______AA_________A_____-5'\n_____________\|______________________________\|\n_____________29_____________________________1\n	198 -- 204	5 -- 11	-19.4278 kcal/mol	6.77275 kcal/mol	0.766821 kcal/mol	179 211@LB@ \| \|@LB@5'-UUG...CACUG AU AAA CAG CGCAU...GUG-3'@LB@ GGUAA AAUAAGG AGUGAUGA AGC@LB@ \|\|\|\|\| \|\|\|\|\|:\| \|:\|\|\|\|\|\| \|\|\|@LB@ CCAUU UUAUUUC UUACUACU UCG@LB@3'-UUU...UACUG AU AA A -5'@LB@ \| \|@LB@ 29 1@LB@@LB@@LB@Energy -11.89 kcal/mol@LB@Hybridization Energy -19.4278 kcal/mol@LB@Unfolding Energy - mRNA 6.77275 kcal/mol@LB@Unfolding Energy - sRNA 0.766821 kcal/mol@LB@@LB@Position - mRNA 180 -- 210@LB@Position - sRNA 1 -- 28@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 5 -- 11@LB@
172	0.02805	0.531	b1912	pgsA	-10.33	0.083391	160 -- 169	52 -- 61	phosphatidylglycerophosphate synthetase		_____________159________170\n_____________\|__________\|\n5'-GUC...GCAAC__________UCUUA...UUG-3'\n______________AUAGGGGUAA\n______________\|\|\|\|\|\|\|\|\|\|\n______________UAUCCCCAUU\n3'-UUU...AACUU__________UGUCU...UCG-5'\n_____________\|__________\|\n_____________62_________51\n	163 -- 169	52 -- 58	-13.9577 kcal/mol	3.55629 kcal/mol	0.0747282 kcal/mol	159 170@LB@ \| \|@LB@5'-GUC...GCAAC UCUUA...UUG-3'@LB@ AUAGGGGUAA@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ UAUCCCCAUU@LB@3'-UUU...AACUU UGUCU...UCG-5'@LB@ \| \|@LB@ 62 51@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -13.9577 kcal/mol@LB@Unfolding Energy - mRNA 3.55629 kcal/mol@LB@Unfolding Energy - sRNA 0.0747282 kcal/mol@LB@@LB@Position - mRNA 160 -- 169@LB@Position - sRNA 52 -- 61@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 52 -- 58@LB@
74	0.0107	0.4523	b3385	gph	-12.85	0.023515	41 -- 77	8 -- 48	phosphoglycolate phosphatase		_____________40____________________________________________78\n_____________\|_____________________________________________\|\n5'-ACU...GCUUU_______GACU__________________C_______________ACAAC...UGU-3'\n______________GACAUUC____AGAAGUG___GACGGUGG___GUGAAG___GUGA\n______________\|\|\|\|\|\|\|____\|\|\|\|:\|\|___\|\|\|\|\|\|::___:\|:\|\|\|___:\|\|\|\n______________CUGUAAG____UCUUUAC___CUGCCAUU___UAUUUC___UACU\n3'-UUU...UUUGU__________________UUA________AUU______AAU____ACUAUCG-5'\n_____________\|_____________________________________________\|\n_____________49____________________________________________7\n	60 -- 66	24 -- 30	-29.3756 kcal/mol	10.2075 kcal/mol	6.31567 kcal/mol	40 78@LB@ \| \|@LB@5'-ACU...GCUUU GACU C ACAAC...UGU-3'@LB@ GACAUUC AGAAGUG GACGGUGG GUGAAG GUGA@LB@ \|\|\|\|\|\|\| \|\|\|\|:\|\| \|\|\|\|\|\|:: :\|:\|\|\| :\|\|\|@LB@ CUGUAAG UCUUUAC CUGCCAUU UAUUUC UACU@LB@3'-UUU...UUUGU UUA AUU AAU ACUAUCG-5'@LB@ \| \|@LB@ 49 7@LB@@LB@@LB@Energy -12.85 kcal/mol@LB@Hybridization Energy -29.3756 kcal/mol@LB@Unfolding Energy - mRNA 10.2075 kcal/mol@LB@Unfolding Energy - sRNA 6.31567 kcal/mol@LB@@LB@Position - mRNA 41 -- 77@LB@Position - sRNA 8 -- 48@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 24 -- 30@LB@
175	0.0283	0.531	b2048	cpsG	-16.42	0.002969	89 -- 138	17 -- 58	phosphomannomutase		_____________88___________________________________________________139\n_____________\|____________________________________________________\|\n5'-GGG...CGGAC___U_______UC_________C________CGAAACC______________CGACA...GCG-3'\n______________GGG_GUAAACG__GCA_UCAGG_AAUGAAUG_______GCGGU_GUAAAUAA\n______________\|\|\|_\|\|\|\|\|\|:__:\|\|_\|\|\|\|:_\|\|\|\|\|\|\|\|_______:\|\|\|\|_:\|\|\|\|\|\|\|\n______________CCC_CAUUUGU__UGU_AGUCU_UUACUUAC_______UGCCA_UAUUUAUU\n3'-UUU...UUUAU___________C____A__________________________U________UCAAU...UCG-5'\n_____________\|____________________________________________________\|\n_____________59___________________________________________________16\n	132 -- 138	17 -- 23	-37.1596 kcal/mol	14.4139 kcal/mol	6.32276 kcal/mol	88 139@LB@ \| \|@LB@5'-GGG...CGGAC U UC C CGAAACC CGACA...GCG-3'@LB@ GGG GUAAACG GCA UCAGG AAUGAAUG GCGGU GUAAAUAA@LB@ \|\|\| \|\|\|\|\|\|: :\|\| \|\|\|\|: \|\|\|\|\|\|\|\| :\|\|\|\| :\|\|\|\|\|\|\|@LB@ CCC CAUUUGU UGU AGUCU UUACUUAC UGCCA UAUUUAUU@LB@3'-UUU...UUUAU C A U UCAAU...UCG-5'@LB@ \| \|@LB@ 59 16@LB@@LB@@LB@Energy -16.42 kcal/mol@LB@Hybridization Energy -37.1596 kcal/mol@LB@Unfolding Energy - mRNA 14.4139 kcal/mol@LB@Unfolding Energy - sRNA 6.32276 kcal/mol@LB@@LB@Position - mRNA 89 -- 138@LB@Position - sRNA 17 -- 58@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 17 -- 23@LB@
48	0.005342	0.3653	b3380	yhfW	-15.20	0.006283	218 -- 233	1 -- 15	phosphopentomutase-related metalloenzyme superfamily protein		_____________217______________234\n_____________\|________________\|\n5'-CUC...GUGGU________U_______UUUGG...GUG-3'\n______________GUUAGUGA_UGAUAGC\n______________\|\|\|\|:\|\|\|_\|\|\|\|\|\|\|\n______________CAAUUACU_ACUAUCG\n3'-UUU...UAUUU________________-5'\n_____________\|_______________\|\n_____________16______________1\n	227 -- 233	1 -- 7	-17.7091 kcal/mol	2.46465 kcal/mol	0.0474282 kcal/mol	217 234@LB@ \| \|@LB@5'-CUC...GUGGU U UUUGG...GUG-3'@LB@ GUUAGUGA UGAUAGC@LB@ \|\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ CAAUUACU ACUAUCG@LB@3'-UUU...UAUUU -5'@LB@ \| \|@LB@ 16 1@LB@@LB@@LB@Energy -15.20 kcal/mol@LB@Hybridization Energy -17.7091 kcal/mol@LB@Unfolding Energy - mRNA 2.46465 kcal/mol@LB@Unfolding Energy - sRNA 0.0474282 kcal/mol@LB@@LB@Position - mRNA 218 -- 233@LB@Position - sRNA 1 -- 15@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 1 -- 7@LB@
36	0.003415	0.3114	b1849	purT	-10.41	0.080237	8 -- 43	29 -- 56	phosphoribosylglycinamide formyltransferase 2		_____________7_____________________________________44\n_____________\|_____________________________________\|\n____5'-UUUUGCU_______________UCUCUUCGCCCUCAC_______UACGU...GUC-3'\n______________GGUAAAC_GACAUUU_______________GAAUGAC\n______________\|\|\|\|\|\|\|_\|\|\|\|\|\|:_______________\|\|\|\|\|\|\|\n______________CCAUUUG_CUGUAAG_______________CUUACUG\n3'-UUU...UAUCC_______U_______UCUUUA________________CCAUU...UCG-5'\n_____________\|_____________________________________\|\n_____________57____________________________________28\n	37 -- 43	29 -- 35	-21.896 kcal/mol	5.20104 kcal/mol	6.28612 kcal/mol	7 44@LB@ \| \|@LB@ 5'-UUUUGCU UCUCUUCGCCCUCAC UACGU...GUC-3'@LB@ GGUAAAC GACAUUU GAAUGAC@LB@ \|\|\|\|\|\|\| \|\|\|\|\|\|: \|\|\|\|\|\|\|@LB@ CCAUUUG CUGUAAG CUUACUG@LB@3'-UUU...UAUCC U UCUUUA CCAUU...UCG-5'@LB@ \| \|@LB@ 57 28@LB@@LB@@LB@Energy -10.41 kcal/mol@LB@Hybridization Energy -21.896 kcal/mol@LB@Unfolding Energy - mRNA 5.20104 kcal/mol@LB@Unfolding Energy - sRNA 6.28612 kcal/mol@LB@@LB@Position - mRNA 8 -- 43@LB@Position - sRNA 29 -- 56@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 29 -- 35@LB@
20	0.0009027	0.1482	b2696	csrA	-13.42	0.017327	268 -- 280	48 -- 60	pleiotropic regulatory protein for carbon source metabolism		_____________267___________281\n_____________\|_____________\|\n5'-CGG...AGUUU__________G__CAACC...GCG-3'\n______________UAGGGGUAAA_GG\n______________\|\|\|\|\|\|\|\|\|\|_:\|\n______________AUCCCCAUUU_UC\n3'-UUU...ACUUU__________G__UGUAA...UCG-5'\n_____________\|_____________\|\n_____________61____________47\n	271 -- 277	51 -- 57	-17.9026 kcal/mol	2.43021 kcal/mol	2.05446 kcal/mol	267 281@LB@ \| \|@LB@5'-CGG...AGUUU G CAACC...GCG-3'@LB@ UAGGGGUAAA GG@LB@ \|\|\|\|\|\|\|\|\|\| :\|@LB@ AUCCCCAUUU UC@LB@3'-UUU...ACUUU G UGUAA...UCG-5'@LB@ \| \|@LB@ 61 47@LB@@LB@@LB@Energy -13.42 kcal/mol@LB@Hybridization Energy -17.9026 kcal/mol@LB@Unfolding Energy - mRNA 2.43021 kcal/mol@LB@Unfolding Energy - sRNA 2.05446 kcal/mol@LB@@LB@Position - mRNA 268 -- 280@LB@Position - sRNA 48 -- 60@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 51 -- 57@LB@
59	0.008416	0.4523	b0566	envY	-9.46	0.123665	249 -- 263	50 -- 64	porin thermoregulatory transcriptional activator	b1499 b0564 b3515 b4116 b3516	_____________248_____________264\n_____________\|_______________\|\n5'-GGC...AAAAA_______A_______GUCAA...ACU-3'\n______________GAGGUAG_GGUAAGC\n______________\|\|::\|\|\|_\|\|\|\|\|:\|\n______________CUUUAUC_CCAUUUG\n3'-UUU...GCCAA_______C_______UCUGU...UCG-5'\n_____________\|_______________\|\n_____________65______________49\n	257 -- 263	50 -- 56	-15.2892 kcal/mol	5.08919 kcal/mol	0.736811 kcal/mol	248 264@LB@ \| \|@LB@5'-GGC...AAAAA A GUCAA...ACU-3'@LB@ GAGGUAG GGUAAGC@LB@ \|\|::\|\|\| \|\|\|\|\|:\|@LB@ CUUUAUC CCAUUUG@LB@3'-UUU...GCCAA C UCUGU...UCG-5'@LB@ \| \|@LB@ 65 49@LB@@LB@@LB@Energy -9.46 kcal/mol@LB@Hybridization Energy -15.2892 kcal/mol@LB@Unfolding Energy - mRNA 5.08919 kcal/mol@LB@Unfolding Energy - sRNA 0.736811 kcal/mol@LB@@LB@Position - mRNA 249 -- 263@LB@Position - sRNA 50 -- 64@LB@Position Seed - mRNA 257 -- 263@LB@Position Seed - sRNA 50 -- 56@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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