Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
18	0.0008759	0.1482	b3626	waaR	-11.62	0.044565	224 -- 238	11 -- 25	lipopolysaccharide 1 2-glucosyltransferase; UDP-glucose:(glucosyl)LPS alpha-1 2-glucosyltransferase	_____________223_____________239\n_____________\|_______________\|\n5'-UGG...AUAGA_______________AGCCU...GUG-3'\n______________GAUAGAUAAAGUUAA\n______________:\|\|\|:\|\|\|\|\|\|\|\|\|\|\n______________UUAUUUAUUUCAAUU\n3'-UUU...UGCCA_______________ACUAC...UCG-5'\n_____________\|_______________\|\n_____________26______________10\n	232 -- 238	11 -- 17	-12.9968 kcal/mol	1.27318 kcal/mol	0.10636 kcal/mol	223 239@LB@ \| \|@LB@5'-UGG...AUAGA AGCCU...GUG-3'@LB@ GAUAGAUAAAGUUAA@LB@ :\|\|\|:\|\|\|\|\|\|\|\|\|\|@LB@ UUAUUUAUUUCAAUU@LB@3'-UUU...UGCCA ACUAC...UCG-5'@LB@ \| \|@LB@ 26 10@LB@@LB@@LB@Energy -11.62 kcal/mol@LB@Hybridization Energy -12.9968 kcal/mol@LB@Unfolding Energy - mRNA 1.27318 kcal/mol@LB@Unfolding Energy - sRNA 0.10636 kcal/mol@LB@@LB@Position - mRNA 224 -- 238@LB@Position - sRNA 11 -- 25@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 11 -- 17@LB@
142	0.02395	0.526	b0192	nlpE	-14.44	0.009771	167 -- 226	1 -- 55	lipoprotein involved with copper homeostasis and adhesion	_____________166_______________________________________________________________227\n_____________\|_________________________________________________________________\|\n5'-UCA...GCAAA___C_______G________AAAAAGAAG____G________AA_______C____A____C___GAUGG...CGC-3'\n______________GUG_GCAG_CG_UCGGGAAU_________GAAU_GAUGGUGA_____AAAG__GAU_GUGA_AGC\n______________\|\|:_:\|\|\|_\|:_\|\|:\|:\|\|\|_________\|\|\|\|_\|\|:\|\|\|:\|_____\|\|\|\|__:\|\|_:\|\|\|_\|\|\|\n______________CAU_UGUC_GU_AGUCUUUA_________CUUA_CUGCCAUU_____UUUC__UUA_UACU_UCG\n3'-UUU...AUCCC___U____U__A______________________________AUUUA____AA___C____A___-5'\n_____________\|________________________________________________________________\|\n_____________56_______________________________________________________________1\n	201 -- 207	24 -- 30	-31.0616 kcal/mol	10.2836 kcal/mol	6.33758 kcal/mol	166 227@LB@ \| \|@LB@5'-UCA...GCAAA C G AAAAAGAAG G AA C A C GAUGG...CGC-3'@LB@ GUG GCAG CG UCGGGAAU GAAU GAUGGUGA AAAG GAU GUGA AGC@LB@ \|\|: :\|\|\| \|: \|\|:\|:\|\|\| \|\|\|\| \|\|:\|\|\|:\| \|\|\|\| :\|\| :\|\|\| \|\|\|@LB@ CAU UGUC GU AGUCUUUA CUUA CUGCCAUU UUUC UUA UACU UCG@LB@3'-UUU...AUCCC U U A AUUUA AA C A -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -14.44 kcal/mol@LB@Hybridization Energy -31.0616 kcal/mol@LB@Unfolding Energy - mRNA 10.2836 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 167 -- 226@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 24 -- 30@LB@
140	0.02319	0.526	b3615	waaH	-10.58	0.073933	178 -- 212	1 -- 47	LPS(HepIII)-glucuronic acid glycosyltransferase	_____________177______________________________________________213\n_____________\|________________________________________________\|\n5'-GAU...GGAUA____________C____U_______UU________________AC___ACCAA...GGA-3'\n______________AC_UUCAGAGGU____G_CGGUAAU___________AUGAUGA__AGC\n______________\|\|_\|\|\|\|\|\|::\|____\|_\|\|\|\|\|\|\|___________\|\|\|\|\|\|\|__\|\|\|\n______________UG_AAGUCUUUA____C_GCCAUUA___________UACUACU__UCG\n3'-UUU...UUGUC__U_________CUUA_U_______UUUAUUUCAAU_______A____-5'\n_____________\|_______________________________________________\|\n_____________48______________________________________________1\n	201 -- 207	5 -- 11	-24.9941 kcal/mol	8.09222 kcal/mol	6.32011 kcal/mol	177 213@LB@ \| \|@LB@5'-GAU...GGAUA C U UU AC ACCAA...GGA-3'@LB@ AC UUCAGAGGU G CGGUAAU AUGAUGA AGC@LB@ \|\| \|\|\|\|\|\|::\| \| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ UG AAGUCUUUA C GCCAUUA UACUACU UCG@LB@3'-UUU...UUGUC U CUUA U UUUAUUUCAAU A -5'@LB@ \| \|@LB@ 48 1@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -24.9941 kcal/mol@LB@Unfolding Energy - mRNA 8.09222 kcal/mol@LB@Unfolding Energy - sRNA 6.32011 kcal/mol@LB@@LB@Position - mRNA 178 -- 212@LB@Position - sRNA 1 -- 47@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 5 -- 11@LB@
40	0.004127	0.3349	b1659	ydhB	-12.78	0.024485	130 -- 177	1 -- 61	LysR family putative transcriptional regulator	_____________129_______________________________________________________________178\n_____________\|_________________________________________________________________\|\n5'-CGA...AAAGU______A_____AU_____________________________UU_______________C____UUCAA...UCA-3'\n______________GUAGGG_GUGAA__UAG_UGUU_____GUGAA__ACGGUAAUA___________UGAUGA_UGGU\n______________:\|\|\|\|\|_\|\|:\|\|__:\|\|_::\|\|_____:\|\|\|\|__\|\|\|\|\|\|\|\|\|___________\|\|\|\|\|\|_\|:\|:\n______________UAUCCC_CAUUU__GUC_GUAA_____UACUU__UGCCAUUAU___________ACUACU_AUCG\n3'-UUU...AACUU_________________U____GUCUU_____AC_________UUAUUUCAAUU___________-5'\n_____________\|________________________________________________________________\|\n_____________62_______________________________________________________________1\n	158 -- 164	22 -- 28	-31.01 kcal/mol	11.8895 kcal/mol	6.344 kcal/mol	129 178@LB@ \| \|@LB@5'-CGA...AAAGU A AU UU C UUCAA...UCA-3'@LB@ GUAGGG GUGAA UAG UGUU GUGAA ACGGUAAUA UGAUGA UGGU@LB@ :\|\|\|\|\| \|\|:\|\| :\|\| ::\|\| :\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|:\|:@LB@ UAUCCC CAUUU GUC GUAA UACUU UGCCAUUAU ACUACU AUCG@LB@3'-UUU...AACUU U GUCUU AC UUAUUUCAAUU -5'@LB@ \| \|@LB@ 62 1@LB@@LB@@LB@Energy -12.78 kcal/mol@LB@Hybridization Energy -31.01 kcal/mol@LB@Unfolding Energy - mRNA 11.8895 kcal/mol@LB@Unfolding Energy - sRNA 6.344 kcal/mol@LB@@LB@Position - mRNA 130 -- 177@LB@Position - sRNA 1 -- 61@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 22 -- 28@LB@
69	0.009861	0.4523	b0879	macB	-12.35	0.030572	144 -- 182	1 -- 59	macrolide ABC transporter peremase/ATPase	_____________143___________________________________________________________183\n_____________\|_____________________________________________________________\|\n5'-AAU...GUCAA____CUUG_____________________________________________________CAAAC...CGG-3'\n______________AGGG____AAGCGGGC_______GAUGAA_G_UGGUGAU____________UGGUGA_GGC\n______________\|\|\|\|____\|\|:\|:\|:\|_______:\|\|\|\|\|_\|_:\|\|\|:\|\|____________\|\|:\|\|\|_:\|\|\n______________UCCC____UUUGUCUG_______UUACUU_C_GCCAUUA____________ACUACU_UCG\n3'-UUU...CUUUA____CA__________UAAGUCU______A_U_______UUUAUUUCAAUU______A___-5'\n_____________\|____________________________________________________________\|\n_____________60___________________________________________________________1\n	167 -- 173	23 -- 29	-29.0987 kcal/mol	10.4067 kcal/mol	6.33764 kcal/mol	143 183@LB@ \| \|@LB@5'-AAU...GUCAA CUUG CAAAC...CGG-3'@LB@ AGGG AAGCGGGC GAUGAA G UGGUGAU UGGUGA GGC@LB@ \|\|\|\| \|\|:\|:\|:\| :\|\|\|\|\| \| :\|\|\|:\|\| \|\|:\|\|\| :\|\|@LB@ UCCC UUUGUCUG UUACUU C GCCAUUA ACUACU UCG@LB@3'-UUU...CUUUA CA UAAGUCU A U UUUAUUUCAAUU A -5'@LB@ \| \|@LB@ 60 1@LB@@LB@@LB@Energy -12.35 kcal/mol@LB@Hybridization Energy -29.0987 kcal/mol@LB@Unfolding Energy - mRNA 10.4067 kcal/mol@LB@Unfolding Energy - sRNA 6.33764 kcal/mol@LB@@LB@Position - mRNA 144 -- 182@LB@Position - sRNA 1 -- 59@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 23 -- 29@LB@
29	0.00201	0.2275	b1479	maeA	-13.16	0.019904	170 -- 200	29 -- 59	malate dehydrogenase decarboxylating NAD-requiring; malic enzyme	_____________169_________________________________201\n_____________\|___________________________________\|\n5'-UAG...CCCCC___________________AAAAA___________AUGGA...GCA-3'\n______________AGGG____AUGGAUAUUCA_____GAGUGAGUGAC\n______________\|\|\|\|____\|::\|\|:\|\|\|\|\|_____:\|:\|\|\|:\|\|\|\|\n______________UCCC____UGUCUGUAAGU_____UUUACUUACUG\n3'-UUU...CUUUA____CAUU___________C_______________CCAUU...UCG-5'\n_____________\|___________________________________\|\n_____________60__________________________________28\n	194 -- 200	29 -- 35	-27.1811 kcal/mol	7.73175 kcal/mol	6.28641 kcal/mol	169 201@LB@ \| \|@LB@5'-UAG...CCCCC AAAAA AUGGA...GCA-3'@LB@ AGGG AUGGAUAUUCA GAGUGAGUGAC@LB@ \|\|\|\| \|::\|\|:\|\|\|\|\| :\|:\|\|\|:\|\|\|\|@LB@ UCCC UGUCUGUAAGU UUUACUUACUG@LB@3'-UUU...CUUUA CAUU C CCAUU...UCG-5'@LB@ \| \|@LB@ 60 28@LB@@LB@@LB@Energy -13.16 kcal/mol@LB@Hybridization Energy -27.1811 kcal/mol@LB@Unfolding Energy - mRNA 7.73175 kcal/mol@LB@Unfolding Energy - sRNA 6.28641 kcal/mol@LB@@LB@Position - mRNA 170 -- 200@LB@Position - sRNA 29 -- 59@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 29 -- 35@LB@
66	0.009484	0.4523	b4034	malE	-12.98	0.021943	73 -- 123	2 -- 55	maltose transporter subunit	_____________72_____________________________________________________124\n_____________\|______________________________________________________\|\n5'-AGG...AUUCU_________G_____CACAAAGC_________GGC______GC__G__G_____AAAGA...UGG-3'\n______________GU_AACAGA_A_UCA_________GACGGUGG___GUAGGG__AA_GA_GAUGG\n______________\|\|_\|\|\|\|\|\|_\|_\|\|\|_________\|\|\|\|\|\|::___:\|\|::\|__\|\|_\|\|_\|\|\|:\|\n______________CA_UUGUCU_U_AGU_________CUGCCAUU___UAUUUC__UU_CU_CUAUC\n3'-UUU...AUCCC__U______G_A___CUUUACUUA________AUU______AA__A__A_____G-5'\n_____________\|______________________________________________________\|\n_____________56_____________________________________________________1\n	95 -- 101	24 -- 30	-30.2337 kcal/mol	10.9143 kcal/mol	6.33752 kcal/mol	72 124@LB@ \| \|@LB@5'-AGG...AUUCU G CACAAAGC GGC GC G G AAAGA...UGG-3'@LB@ GU AACAGA A UCA GACGGUGG GUAGGG AA GA GAUGG@LB@ \|\| \|\|\|\|\|\| \| \|\|\| \|\|\|\|\|\|:: :\|\|::\| \|\| \|\| \|\|\|:\|@LB@ CA UUGUCU U AGU CUGCCAUU UAUUUC UU CU CUAUC@LB@3'-UUU...AUCCC U G A CUUUACUUA AUU AA A A G-5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -12.98 kcal/mol@LB@Hybridization Energy -30.2337 kcal/mol@LB@Unfolding Energy - mRNA 10.9143 kcal/mol@LB@Unfolding Energy - sRNA 6.33752 kcal/mol@LB@@LB@Position - mRNA 73 -- 123@LB@Position - sRNA 2 -- 55@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 24 -- 30@LB@
63	0.008816	0.4523	b3600	mtlD	-11.23	0.053963	184 -- 208	1 -- 23	mannitol-1-phosphate dehydrogenase NAD-dependent	_____________183_______________________209\n_____________\|_________________________\|\n5'-GUG...AACAU___UG_________ACU____A___AUUAC...AUC-3'\n______________UGA__AAGGUUAAU___AUGA_AGC\n______________\|:\|__\|\|:\|\|\|\|\|\|___\|\|\|\|_\|\|\|\n______________AUU__UUUCAAUUA___UACU_UCG\n3'-UUU...CCAUU___UA_________C______A___-5'\n_____________\|________________________\|\n_____________24_______________________1\n	191 -- 197	10 -- 16	-14.8386 kcal/mol	3.50103 kcal/mol	0.105066 kcal/mol	183 209@LB@ \| \|@LB@5'-GUG...AACAU UG ACU A AUUAC...AUC-3'@LB@ UGA AAGGUUAAU AUGA AGC@LB@ \|:\| \|\|:\|\|\|\|\|\| \|\|\|\| \|\|\|@LB@ AUU UUUCAAUUA UACU UCG@LB@3'-UUU...CCAUU UA C A -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -11.23 kcal/mol@LB@Hybridization Energy -14.8386 kcal/mol@LB@Unfolding Energy - mRNA 3.50103 kcal/mol@LB@Unfolding Energy - sRNA 0.105066 kcal/mol@LB@@LB@Position - mRNA 184 -- 208@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 10 -- 16@LB@
194	0.03269	0.5507	b2049	cpsB	-9.94	0.099932	179 -- 235	1 -- 54	mannose-1-phosphate guanyltransferase	_____________178________________________________________________________236\n_____________\|__________________________________________________________\|\n5'-ACA...AUUUA___________________AAUU______CA__CGAAACUCUAUCC___U________AGGUG...GCC-3'\n______________UAAAU_GAUAUUCGGGGAU____AUGGCG__GU_____________AGU_GUGAUGGC\n______________\|\|\|\|:_\|\|:\|\|\|\|:\|::\|\|____\|\|\|:\|\|__\|\|_____________\|:\|_:\|\|\|\|:\|\|\n______________AUUUG_CUGUAAGUCUUUA____UACUGC__CA_____________UUA_UACUAUCG\n3'-UUU...UCCCC_____U_____________CU____________UUAUUUAUUUCAA___C________-5'\n_____________\|_________________________________________________________\|\n_____________55________________________________________________________1\n	229 -- 235	1 -- 7	-30.0746 kcal/mol	13.8011 kcal/mol	6.33758 kcal/mol	178 236@LB@ \| \|@LB@5'-ACA...AUUUA AAUU CA CGAAACUCUAUCC U AGGUG...GCC-3'@LB@ UAAAU GAUAUUCGGGGAU AUGGCG GU AGU GUGAUGGC@LB@ \|\|\|\|: \|\|:\|\|\|\|:\|::\|\| \|\|\|:\|\| \|\| \|:\| :\|\|\|\|:\|\|@LB@ AUUUG CUGUAAGUCUUUA UACUGC CA UUA UACUAUCG@LB@3'-UUU...UCCCC U CU UUAUUUAUUUCAA C -5'@LB@ \| \|@LB@ 55 1@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -30.0746 kcal/mol@LB@Unfolding Energy - mRNA 13.8011 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 179 -- 235@LB@Position - sRNA 1 -- 54@LB@Position Seed - mRNA 229 -- 235@LB@Position Seed - sRNA 1 -- 7@LB@
167	0.02708	0.531	b3705	yidC	-10.20	0.088356	85 -- 115	24 -- 58	membrane protein insertase	_____________84____________________________________116\n_____________\|_____________________________________\|\n5'-CGC...GGUUU__A____UAAA_____GU_________U_________AACGC...CCC-3'\n______________GG_GUGA____AGGCA________UGG_UGACGGUGA\n______________\|\|_\|\|:\|____\|\|:\|\|________\|\|:_\|\|\|\|\|\|\|:\|\n______________CC_CAUU____UCUGU________ACU_ACUGCCAUU\n3'-UUU...UUUAU__C____UG_______AAGUCUUU___U_________AUUUA...UCG-5'\n_____________\|_____________________________________\|\n_____________59____________________________________23\n	109 -- 115	24 -- 30	-23.9536 kcal/mol	7.45063 kcal/mol	6.30019 kcal/mol	84 116@LB@ \| \|@LB@5'-CGC...GGUUU A UAAA GU U AACGC...CCC-3'@LB@ GG GUGA AGGCA UGG UGACGGUGA@LB@ \|\| \|\|:\| \|\|:\|\| \|\|: \|\|\|\|\|\|\|:\|@LB@ CC CAUU UCUGU ACU ACUGCCAUU@LB@3'-UUU...UUUAU C UG AAGUCUUU U AUUUA...UCG-5'@LB@ \| \|@LB@ 59 23@LB@@LB@@LB@Energy -10.20 kcal/mol@LB@Hybridization Energy -23.9536 kcal/mol@LB@Unfolding Energy - mRNA 7.45063 kcal/mol@LB@Unfolding Energy - sRNA 6.30019 kcal/mol@LB@@LB@Position - mRNA 85 -- 115@LB@Position - sRNA 24 -- 58@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 24 -- 30@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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