Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
184	0.03077	0.549	b1101	ptsG	-13.95	0.012872	39 -- 58	1 -- 19	fused glucose-specific PTS enzymes: IIB component/IIC component	_____________38___________________59\n_____________\|____________________\|\n5'-CAU...UGAUC_________AAC________CGUCA...GUU-3'\n______________UGAAGUUGA___GUGAUAGC\n______________\|:\|\|\|\|\|:\|___:\|\|\|\|\|\|\|\n______________AUUUCAAUU___UACUAUCG\n3'-UUU...UAUUU_________AC_________-5'\n_____________\|___________________\|\n_____________20__________________1\n	52 -- 58	1 -- 7	-17.415 kcal/mol	3.39679 kcal/mol	0.0638423 kcal/mol	38 59@LB@ \| \|@LB@5'-CAU...UGAUC AAC CGUCA...GUU-3'@LB@ UGAAGUUGA GUGAUAGC@LB@ \|:\|\|\|\|\|:\| :\|\|\|\|\|\|\|@LB@ AUUUCAAUU UACUAUCG@LB@3'-UUU...UAUUU AC -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -13.95 kcal/mol@LB@Hybridization Energy -17.415 kcal/mol@LB@Unfolding Energy - mRNA 3.39679 kcal/mol@LB@Unfolding Energy - sRNA 0.0638423 kcal/mol@LB@@LB@Position - mRNA 39 -- 58@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 1 -- 7@LB@
165	0.02655	0.526	b2388	glk	-7.68	0.259430	184 -- 233	1 -- 52	glucokinase	_____________183_____________________________________________________234\n_____________\|_______________________________________________________\|\n5'-CUA...ACUUU______G__GUU_____________AAA___UGCAUU_____C_______UG___GGCAC...AUU-3'\n______________AGCGGA_CA____GAA__GAAUGAC___GUA________AGU_GGUGAUG__GGC\n______________\|:\|:\|\|_\|\|____\|\|\|__\|\|\|\|\|\|\|___\|\|\|________\|\|\|_::\|\|\|\|\|__:\|\|\n______________UUGUCU_GU____CUU__CUUACUG___CAU________UCA_UUACUAC__UCG\n3'-UUU...CCCAU_________AAGU___UA_______C_____UAUUUAUU___A_______UA___-5'\n_____________\|______________________________________________________\|\n_____________53_____________________________________________________1\n	222 -- 228	6 -- 12	-28.455 kcal/mol	14.4434 kcal/mol	6.33555 kcal/mol	183 234@LB@ \| \|@LB@5'-CUA...ACUUU G GUU AAA UGCAUU C UG GGCAC...AUU-3'@LB@ AGCGGA CA GAA GAAUGAC GUA AGU GGUGAUG GGC@LB@ \|:\|:\|\| \|\| \|\|\| \|\|\|\|\|\|\| \|\|\| \|\|\| ::\|\|\|\|\| :\|\|@LB@ UUGUCU GU CUU CUUACUG CAU UCA UUACUAC UCG@LB@3'-UUU...CCCAU AAGU UA C UAUUUAUU A UA -5'@LB@ \| \|@LB@ 53 1@LB@@LB@@LB@Energy -7.68 kcal/mol@LB@Hybridization Energy -28.455 kcal/mol@LB@Unfolding Energy - mRNA 14.4434 kcal/mol@LB@Unfolding Energy - sRNA 6.33555 kcal/mol@LB@@LB@Position - mRNA 184 -- 233@LB@Position - sRNA 1 -- 52@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 6 -- 12@LB@
195	0.03271	0.5507	b3213	gltD	-10.79	0.067022	188 -- 211	38 -- 59	glutamate synthase 4Fe-4S protein small subunit	_____________187______________________212\n_____________\|________________________\|\n5'-AGC...CAGUA_______GC____AUGAG______UGUUU...AGC-3'\n______________AGGGGUA__AACA_____UCAGAA\n______________\|\|\|\|\|\|\|__\|\|\|\|_____\|\|\|\|\|\|\n______________UCCCCAU__UUGU_____AGUCUU\n3'-UUU...CUUUA_____________CUGUA______UACUU...UCG-5'\n_____________\|________________________\|\n_____________60_______________________37\n	188 -- 194	53 -- 59	-20.1632 kcal/mol	3.12506 kcal/mol	6.25108 kcal/mol	187 212@LB@ \| \|@LB@5'-AGC...CAGUA GC AUGAG UGUUU...AGC-3'@LB@ AGGGGUA AACA UCAGAA@LB@ \|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|@LB@ UCCCCAU UUGU AGUCUU@LB@3'-UUU...CUUUA CUGUA UACUU...UCG-5'@LB@ \| \|@LB@ 60 37@LB@@LB@@LB@Energy -10.79 kcal/mol@LB@Hybridization Energy -20.1632 kcal/mol@LB@Unfolding Energy - mRNA 3.12506 kcal/mol@LB@Unfolding Energy - sRNA 6.25108 kcal/mol@LB@@LB@Position - mRNA 188 -- 211@LB@Position - sRNA 38 -- 59@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 53 -- 59@LB@
15	0.0006204	0.1358	b0652	gltL	-11.66	0.043639	269 -- 295	1 -- 25	glutamate/aspartate ABC transporter ATPase	_____________268_________________________296\n_____________\|___________________________\|\n5'-CUC...ACCGA______A____GCGA____________UUGCG-3'\n______________AGUGAA_AAAG____AGUGGUGGUGGU\n______________\|:\|:\|\|_\|\|\|\|____\|:\|\|:\|\|:\|:\|:\n______________UUAUUU_UUUC____UUACUACUAUCG\n3'-UUU...UGCCA______A____AA______________-5'\n_____________\|__________________________\|\n_____________26_________________________1\n	289 -- 295	1 -- 7	-17.2962 kcal/mol	5.52495 kcal/mol	0.112205 kcal/mol	268 296@LB@ \| \|@LB@5'-CUC...ACCGA A GCGA UUGCG-3'@LB@ AGUGAA AAAG AGUGGUGGUGGU@LB@ \|:\|:\|\| \|\|\|\| \|:\|\|:\|\|:\|:\|:@LB@ UUAUUU UUUC UUACUACUAUCG@LB@3'-UUU...UGCCA A AA -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -17.2962 kcal/mol@LB@Unfolding Energy - mRNA 5.52495 kcal/mol@LB@Unfolding Energy - sRNA 0.112205 kcal/mol@LB@@LB@Position - mRNA 269 -- 295@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 1 -- 7@LB@
107	0.01649	0.5059	b0830	gsiB	-12.67	0.025954	282 -- 298	1 -- 18	glutathione ABC transporter periplasmic binding protein	_____________281________________299\n_____________\|__________________\|\n5'-GGC...CUGCC____AU____________GG-3'\n______________AAAG___GUGGUGGUGGC\n______________\|\|\|\|___:\|\|:\|\|:\|:\|\|\n______________UUUC___UACUACUAUCG\n3'-UUU...AUUUA____AAU___________-5'\n_____________\|_________________\|\n_____________19________________1\n	292 -- 298	1 -- 7	-15.5562 kcal/mol	2.82856 kcal/mol	0.0611543 kcal/mol	281 299@LB@ \| \|@LB@5'-GGC...CUGCC AU GG-3'@LB@ AAAG GUGGUGGUGGC@LB@ \|\|\|\| :\|\|:\|\|:\|:\|\|@LB@ UUUC UACUACUAUCG@LB@3'-UUU...AUUUA AAU -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -12.67 kcal/mol@LB@Hybridization Energy -15.5562 kcal/mol@LB@Unfolding Energy - mRNA 2.82856 kcal/mol@LB@Unfolding Energy - sRNA 0.0611543 kcal/mol@LB@@LB@Position - mRNA 282 -- 298@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 1 -- 7@LB@
78	0.01113	0.4523	b2678	proW	-13.08	0.020853	190 -- 200	49 -- 59	glycine betaine/proline ABC transporter permease	_____________189_________201\n_____________\|___________\|\n5'-GUU...CGUGA___________AUGGC...ACG-3'\n______________GGGGGUAAAUA\n______________:\|\|\|\|\|\|\|\|:\|\n______________UCCCCAUUUGU\n3'-UUU...CUUUA___________CUGUA...UCG-5'\n_____________\|___________\|\n_____________60__________48\n	194 -- 200	49 -- 55	-15.0309 kcal/mol	1.22377 kcal/mol	0.730523 kcal/mol	189 201@LB@ \| \|@LB@5'-GUU...CGUGA AUGGC...ACG-3'@LB@ GGGGGUAAAUA@LB@ :\|\|\|\|\|\|\|\|:\|@LB@ UCCCCAUUUGU@LB@3'-UUU...CUUUA CUGUA...UCG-5'@LB@ \| \|@LB@ 60 48@LB@@LB@@LB@Energy -13.08 kcal/mol@LB@Hybridization Energy -15.0309 kcal/mol@LB@Unfolding Energy - mRNA 1.22377 kcal/mol@LB@Unfolding Energy - sRNA 0.730523 kcal/mol@LB@@LB@Position - mRNA 190 -- 200@LB@Position - sRNA 49 -- 59@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 49 -- 55@LB@
163	0.02649	0.526	b3156	yhbS	-11.68	0.043230	178 -- 217	16 -- 53	GNAT family putative N-acetyltransferase	_____________177_______________________________________218\n_____________\|_________________________________________\|\n5'-CCG...AAACC______________U_______CC___C____UC_______AAUUC...AUC-3'\n______________AAACAGACA_UCGG_AGGUGAA__AUG_UAAU__GAGUAGA\n______________\|\|\|\|\|\|\|\|\|_\|\|:\|_\|::\|\|\|\|__\|:\|_\|\|\|\|__:\|:\|\|:\|\n______________UUUGUCUGU_AGUC_UUUACUU__UGC_AUUA__UUUAUUU\n3'-UUU...CCCCA_________A____________AC___C_____________CAAUU...UCG-5'\n_____________\|_________________________________________\|\n_____________54________________________________________15\n	211 -- 217	16 -- 22	-26.1463 kcal/mol	8.14596 kcal/mol	6.32252 kcal/mol	177 218@LB@ \| \|@LB@5'-CCG...AAACC U CC C UC AAUUC...AUC-3'@LB@ AAACAGACA UCGG AGGUGAA AUG UAAU GAGUAGA@LB@ \|\|\|\|\|\|\|\|\| \|\|:\| \|::\|\|\|\| \|:\| \|\|\|\| :\|:\|\|:\|@LB@ UUUGUCUGU AGUC UUUACUU UGC AUUA UUUAUUU@LB@3'-UUU...CCCCA A AC C CAAUU...UCG-5'@LB@ \| \|@LB@ 54 15@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -26.1463 kcal/mol@LB@Unfolding Energy - mRNA 8.14596 kcal/mol@LB@Unfolding Energy - sRNA 6.32252 kcal/mol@LB@@LB@Position - mRNA 178 -- 217@LB@Position - sRNA 16 -- 53@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 16 -- 22@LB@
111	0.01748	0.5155	b4012	yjaB	-9.42	0.126217	155 -- 212	1 -- 59	GNAT-family putative N-acetyltransferase; acetyl coenzyme A-binding protein	_____________154_____________________________________________________________213\n_____________\|_______________________________________________________________\|\n5'-AAG...UUUCA___AAA____U___________UAUAA____________A________AA______UAU____AUUCG...CAG-3'\n______________AGG___AGAC__ACG_UUAGAA_____GAAUGAC__UAA_AGG_AGAG__AAUGGU___UAGU\n______________\|\|\|___\|:\|\|__\|\|:_\|:\|\|\|\|_____\|\|\|\|\|\|\|__\|\|\|_\|::_\|:\|\|__\|\|\|\|:\|___\|\|\|:\n______________UCC___UUUG__UGU_AGUCUU_____CUUACUG__AUU_UUU_UUUC__UUACUA___AUCG\n3'-UUU...CUUUA___CCA____UC___A______UA__________CC___A___A____AA______CU_____-5'\n_____________\|______________________________________________________________\|\n_____________60_____________________________________________________________1\n	180 -- 186	29 -- 35	-23.4815 kcal/mol	7.7268 kcal/mol	6.33764 kcal/mol	154 213@LB@ \| \|@LB@5'-AAG...UUUCA AAA U UAUAA A AA UAU AUUCG...CAG-3'@LB@ AGG AGAC ACG UUAGAA GAAUGAC UAA AGG AGAG AAUGGU UAGU@LB@ \|\|\| \|:\|\| \|\|: \|:\|\|\|\| \|\|\|\|\|\|\| \|\|\| \|:: \|:\|\| \|\|\|\|:\| \|\|\|:@LB@ UCC UUUG UGU AGUCUU CUUACUG AUU UUU UUUC UUACUA AUCG@LB@3'-UUU...CUUUA CCA UC A UA CC A A AA CU -5'@LB@ \| \|@LB@ 60 1@LB@@LB@@LB@Energy -9.42 kcal/mol@LB@Hybridization Energy -23.4815 kcal/mol@LB@Unfolding Energy - mRNA 7.7268 kcal/mol@LB@Unfolding Energy - sRNA 6.33764 kcal/mol@LB@@LB@Position - mRNA 155 -- 212@LB@Position - sRNA 1 -- 59@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 29 -- 35@LB@
10	0.0003219	0.1057	b2727	hypB	-18.91	0.000562	181 -- 230	1 -- 53	GTP hydrolase involved in nickel liganding into hydrogenases	_____________180____________________________________________________231\n_____________\|______________________________________________________\|\n5'-GCC...GAUAG_____________________C____U___CAAC___C_____C______A___AACCU...CGG-3'\n______________AAAUAGAC___CAGGAGUGAG_GAUG_GUA____AUG_GGUUG__GGUGA_GGC\n______________\|\|\|:\|\|\|\|___\|\|\|:\|:\|\|\|:_\|\|:\|_\|\|\|____\|\|:_:\|\|\|:__\|:\|\|\|_:\|\|\n______________UUUGUCUG___GUCUUUACUU_CUGC_CAU____UAU_UCAAU__CUACU_UCG\n3'-UUU...CCCCA________UAA__________A________UAUU___U_____UA_____A___-5'\n_____________\|_____________________________________________________\|\n_____________54____________________________________________________1\n	182 -- 188	46 -- 52	-37.7845 kcal/mol	12.539 kcal/mol	6.33709 kcal/mol	180 231@LB@ \| \|@LB@5'-GCC...GAUAG C U CAAC C C A AACCU...CGG-3'@LB@ AAAUAGAC CAGGAGUGAG GAUG GUA AUG GGUUG GGUGA GGC@LB@ \|\|\|:\|\|\|\| \|\|\|:\|:\|\|\|: \|\|:\| \|\|\| \|\|: :\|\|\|: \|:\|\|\| :\|\|@LB@ UUUGUCUG GUCUUUACUU CUGC CAU UAU UCAAU CUACU UCG@LB@3'-UUU...CCCCA UAA A UAUU U UA A -5'@LB@ \| \|@LB@ 54 1@LB@@LB@@LB@Energy -18.91 kcal/mol@LB@Hybridization Energy -37.7845 kcal/mol@LB@Unfolding Energy - mRNA 12.539 kcal/mol@LB@Unfolding Energy - sRNA 6.33709 kcal/mol@LB@@LB@Position - mRNA 181 -- 230@LB@Position - sRNA 1 -- 53@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 46 -- 52@LB@
6	0.0001423	0.07172	b3183	obgE	-17.58	0.001399	174 -- 220	1 -- 51	GTPase involved in cell partioning and DNA repair	_____________173____________________________________________________221\n_____________\|______________________________________________________\|\n5'-UUU...CCCGC__________________UUU____AG___A__________UGU__________AUCGA...GCC-3'\n______________GCAGGC______GAAUGA___ACGG__AAU_AAAUGAAGUU___UGAUGA_AGC\n______________:\|\|\|:\|______:\|\|\|\|\|___\|\|\|\|__\|\|\|_\|\|\|\|:\|\|\|\|\|___\|\|\|\|\|\|_\|\|\|\n______________UGUCUG______UUUACU___UGCC__UUA_UUUAUUUCAA___ACUACU_UCG\n3'-UUU...CCAUU______UAAGUC______UAC____A_______________UU_______A___-5'\n_____________\|_____________________________________________________\|\n_____________52____________________________________________________1\n	202 -- 208	13 -- 19	-33.7182 kcal/mol	9.79823 kcal/mol	6.33528 kcal/mol	173 221@LB@ \| \|@LB@5'-UUU...CCCGC UUU AG A UGU AUCGA...GCC-3'@LB@ GCAGGC GAAUGA ACGG AAU AAAUGAAGUU UGAUGA AGC@LB@ :\|\|\|:\| :\|\|\|\|\| \|\|\|\| \|\|\| \|\|\|\|:\|\|\|\|\| \|\|\|\|\|\| \|\|\|@LB@ UGUCUG UUUACU UGCC UUA UUUAUUUCAA ACUACU UCG@LB@3'-UUU...CCAUU UAAGUC UAC A UU A -5'@LB@ \| \|@LB@ 52 1@LB@@LB@@LB@Energy -17.58 kcal/mol@LB@Hybridization Energy -33.7182 kcal/mol@LB@Unfolding Energy - mRNA 9.79823 kcal/mol@LB@Unfolding Energy - sRNA 6.33528 kcal/mol@LB@@LB@Position - mRNA 174 -- 220@LB@Position - sRNA 1 -- 51@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 13 -- 19@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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