| 96 |
0.00803 |
0.2778 |
bsu39710 |
iolF |
-10.11 |
0.206009 |
172 -- 191 |
30 -- 53 |
minor myo-inositol transporter IolF |
|
_____________171______________________192\n_____________|________________________|\n5'-GGC...AGUUG__________________AU____AAUGA...CCG-3'\n______________UUGGAUCU_GG_CU__UA__GGGG\n______________|:||||||_|:_||__||__||||\n______________AGCCUAGA_CU_GA__AU__CCCC\n3'-UUU...AAAAA________A__A__CU__CU____AACCC...AUA-5'\n_____________|________________________|\n_____________54_______________________29\n |
173 -- 179 |
46 -- 52 |
-17.6548 kcal/mol |
6.56277 kcal/mol |
0.985344 kcal/mol |
171 192@LB@ | |@LB@5'-GGC...AGUUG AU AAUGA...CCG-3'@LB@ UUGGAUCU GG CU UA GGGG@LB@ |:|||||| |: || || ||||@LB@ AGCCUAGA CU GA AU CCCC@LB@3'-UUU...AAAAA A A CU CU AACCC...AUA-5'@LB@ | |@LB@ 54 29@LB@@LB@@LB@Energy -10.11 kcal/mol@LB@Hybridization Energy -17.6548 kcal/mol@LB@Unfolding Energy - mRNA 6.56277 kcal/mol@LB@Unfolding Energy - sRNA 0.985344 kcal/mol@LB@@LB@Position - mRNA 172 -- 191@LB@Position - sRNA 30 -- 53@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 46 -- 52@LB@ |
| 183 |
0.01776 |
0.3237 |
bsu08550 |
sspK |
-13.38 |
0.060997 |
172 -- 193 |
31 -- 48 |
small acid-soluble spore protein K |
|
_____________171____________________194\n_____________|______________________|\n5'-UUU...CAACU_________C__ACC_______AUAUG...ACG-3'\n______________UUUUGAUCU_GA___UAGGGGG\n______________|:|||||||_||___|||:|||\n______________AGAACUAGA_CU___AUCUCCC\n3'-UUU...AGCCU______________________CAACC...AUA-5'\n_____________|______________________|\n_____________49_____________________30\n |
187 -- 193 |
31 -- 37 |
-20.1262 kcal/mol |
5.7766 kcal/mol |
0.970395 kcal/mol |
171 194@LB@ | |@LB@5'-UUU...CAACU C ACC AUAUG...ACG-3'@LB@ UUUUGAUCU GA UAGGGGG@LB@ |:||||||| || |||:|||@LB@ AGAACUAGA CU AUCUCCC@LB@3'-UUU...AGCCU CAACC...AUA-5'@LB@ | |@LB@ 49 30@LB@@LB@@LB@Energy -13.38 kcal/mol@LB@Hybridization Energy -20.1262 kcal/mol@LB@Unfolding Energy - mRNA 5.7766 kcal/mol@LB@Unfolding Energy - sRNA 0.970395 kcal/mol@LB@@LB@Position - mRNA 172 -- 193@LB@Position - sRNA 31 -- 48@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 3 |
5.299e-07 |
0.0005893 |
bsu18000 |
citB |
-22.45 |
0.000394 |
173 -- 202 |
21 -- 50 |
aconitate hydratase |
|
_____________172______________________________203\n_____________|________________________________|\n5'-UCU...AGAAU____________________AUU____A____GGCAA...CGU-3'\n______________GAUC_UGAUCUGA_AGGGGG___UUGG_GAAU\n______________||||_||||||||_||:|||___||||_|||:\n______________CUAG_ACUAGACU_UCUCCC___AACC_CUUG\n3'-UUU...AAAGC____A________A______C______C____UCUCU...AUA-5'\n_____________|________________________________|\n_____________51_______________________________20\n |
178 -- 184 |
38 -- 44 |
-32.0259 kcal/mol |
6.8093 kcal/mol |
2.76846 kcal/mol |
172 203@LB@ | |@LB@5'-UCU...AGAAU AUU A GGCAA...CGU-3'@LB@ GAUC UGAUCUGA AGGGGG UUGG GAAU@LB@ |||| |||||||| ||:||| |||| |||:@LB@ CUAG ACUAGACU UCUCCC AACC CUUG@LB@3'-UUU...AAAGC A A C C UCUCU...AUA-5'@LB@ | |@LB@ 51 20@LB@@LB@@LB@Energy -22.45 kcal/mol@LB@Hybridization Energy -32.0259 kcal/mol@LB@Unfolding Energy - mRNA 6.8093 kcal/mol@LB@Unfolding Energy - sRNA 2.76846 kcal/mol@LB@@LB@Position - mRNA 173 -- 202@LB@Position - sRNA 21 -- 50@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 39 |
0.001675 |
0.1432 |
bsu23240 |
ypzK |
-16.45 |
0.015090 |
173 -- 197 |
28 -- 52 |
riboflavin biosynthesis protein RibT |
|
_____________172_________________________198\n_____________|___________________________|\n5'-GCG...CAGAC____CA______AU_____________UUUAU...UUA-3'\n______________UGGA__UUUUGG____AUAGAGGGGUU\n______________:|||__|:|||:____|||||||||||\n______________GCCU__AGAACU____UAUCUCCCCAA\n3'-UUU...AAAAA____________AGAC___________CCCCU...AUA-5'\n_____________|___________________________|\n_____________53__________________________27\n |
191 -- 197 |
28 -- 34 |
-23.4842 kcal/mol |
6.04944 kcal/mol |
0.980555 kcal/mol |
172 198@LB@ | |@LB@5'-GCG...CAGAC CA AU UUUAU...UUA-3'@LB@ UGGA UUUUGG AUAGAGGGGUU@LB@ :||| |:|||: |||||||||||@LB@ GCCU AGAACU UAUCUCCCCAA@LB@3'-UUU...AAAAA AGAC CCCCU...AUA-5'@LB@ | |@LB@ 53 27@LB@@LB@@LB@Energy -16.45 kcal/mol@LB@Hybridization Energy -23.4842 kcal/mol@LB@Unfolding Energy - mRNA 6.04944 kcal/mol@LB@Unfolding Energy - sRNA 0.980555 kcal/mol@LB@@LB@Position - mRNA 173 -- 197@LB@Position - sRNA 28 -- 52@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 66 |
0.004158 |
0.2071 |
bsu22770 |
mtrB |
-14.22 |
0.042769 |
173 -- 192 |
31 -- 51 |
transcription attenuation protein MtrB |
|
_____________172___________________193\n_____________|_____________________|\n5'-UAG...CGCCA______AAUAAA_________UGCUA...ACC-3'\n______________GGA_CU______GAUAGAGGG\n______________|||_||______|||||||||\n______________CCU_GA______CUAUCUCCC\n3'-UUU...AAAAG___A__ACUAGA_________CAACC...AUA-5'\n_____________|_____________________|\n_____________52____________________30\n |
186 -- 192 |
31 -- 37 |
-18.8398 kcal/mol |
3.65373 kcal/mol |
0.970589 kcal/mol |
172 193@LB@ | |@LB@5'-UAG...CGCCA AAUAAA UGCUA...ACC-3'@LB@ GGA CU GAUAGAGGG@LB@ ||| || |||||||||@LB@ CCU GA CUAUCUCCC@LB@3'-UUU...AAAAG A ACUAGA CAACC...AUA-5'@LB@ | |@LB@ 52 30@LB@@LB@@LB@Energy -14.22 kcal/mol@LB@Hybridization Energy -18.8398 kcal/mol@LB@Unfolding Energy - mRNA 3.65373 kcal/mol@LB@Unfolding Energy - sRNA 0.970589 kcal/mol@LB@@LB@Position - mRNA 173 -- 192@LB@Position - sRNA 31 -- 51@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 152 |
0.01351 |
0.2966 |
bsu25600 |
comER |
-9.59 |
0.243237 |
173 -- 194 |
30 -- 51 |
ComE operon protein 4 |
|
_____________172____________________195\n_____________|______________________|\n5'-AGC...UUAGA____AAUAGCUCAAA_______AAACC...CAA-3'\n______________GGAU___________GGAGGGG\n______________||||___________:||||||\n______________CCUA___________UCUCCCC\n3'-UUU...AAAAG____GAACUAGACUA_______AACCC...AUA-5'\n_____________|______________________|\n_____________52_____________________29\n |
188 -- 194 |
30 -- 36 |
-16.7526 kcal/mol |
6.18889 kcal/mol |
0.974282 kcal/mol |
172 195@LB@ | |@LB@5'-AGC...UUAGA AAUAGCUCAAA AAACC...CAA-3'@LB@ GGAU GGAGGGG@LB@ |||| :||||||@LB@ CCUA UCUCCCC@LB@3'-UUU...AAAAG GAACUAGACUA AACCC...AUA-5'@LB@ | |@LB@ 52 29@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -16.7526 kcal/mol@LB@Unfolding Energy - mRNA 6.18889 kcal/mol@LB@Unfolding Energy - sRNA 0.974282 kcal/mol@LB@@LB@Position - mRNA 173 -- 194@LB@Position - sRNA 30 -- 51@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 89 |
0.00694 |
0.2588 |
bsu06230 |
iolT |
-9.37 |
0.260314 |
174 -- 193 |
31 -- 49 |
major myo-inositol transporter IolT |
|
_____________173__________________194\n_____________|____________________|\n5'-AAA...UACGC_______AAAA__CU_____AAACA...AUG-3'\n______________AUUUUGA____GA__GGGGG\n______________||:||||____||__|:|||\n______________UAGAACU____CU__CUCCC\n3'-UUU...AAGCC_______AGA___AU_____CAACC...AUA-5'\n_____________|____________________|\n_____________50___________________30\n |
174 -- 180 |
43 -- 49 |
-14.1903 kcal/mol |
3.84886 kcal/mol |
0.970571 kcal/mol |
173 194@LB@ | |@LB@5'-AAA...UACGC AAAA CU AAACA...AUG-3'@LB@ AUUUUGA GA GGGGG@LB@ ||:|||| || |:|||@LB@ UAGAACU CU CUCCC@LB@3'-UUU...AAGCC AGA AU CAACC...AUA-5'@LB@ | |@LB@ 50 30@LB@@LB@@LB@Energy -9.37 kcal/mol@LB@Hybridization Energy -14.1903 kcal/mol@LB@Unfolding Energy - mRNA 3.84886 kcal/mol@LB@Unfolding Energy - sRNA 0.970571 kcal/mol@LB@@LB@Position - mRNA 174 -- 193@LB@Position - sRNA 31 -- 49@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 43 -- 49@LB@ |
| 130 |
0.01149 |
0.2883 |
bsu02620 |
phoD |
-13.55 |
0.056825 |
174 -- 193 |
30 -- 50 |
alkaline phosphatase D |
|
_____________173____________________194\n_____________|______________________|\n5'-GCC...GAGAC____AA___G____G_______AUCUU...CGG-3'\n______________GAUC__UGA___GA_AGAGGGG\n______________||||__|||___||_|||||||\n______________CUAG__ACU___CU_UCUCCCC\n3'-UUU...AAAGC____A____AGA__A_______AACCC...AUA-5'\n_____________|______________________|\n_____________51_____________________29\n |
187 -- 193 |
30 -- 36 |
-18.9864 kcal/mol |
4.46268 kcal/mol |
0.974272 kcal/mol |
173 194@LB@ | |@LB@5'-GCC...GAGAC AA G G AUCUU...CGG-3'@LB@ GAUC UGA GA AGAGGGG@LB@ |||| ||| || |||||||@LB@ CUAG ACU CU UCUCCCC@LB@3'-UUU...AAAGC A AGA A AACCC...AUA-5'@LB@ | |@LB@ 51 29@LB@@LB@@LB@Energy -13.55 kcal/mol@LB@Hybridization Energy -18.9864 kcal/mol@LB@Unfolding Energy - mRNA 4.46268 kcal/mol@LB@Unfolding Energy - sRNA 0.974272 kcal/mol@LB@@LB@Position - mRNA 174 -- 193@LB@Position - sRNA 30 -- 50@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 18 |
0.00036 |
0.06216 |
bsu24510 |
yqhO |
-21.34 |
0.000873 |
175 -- 205 |
13 -- 44 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________174_______________________________206\n_____________|_________________________________|\n5'-UAU...UAAAC____G__________GA____________U___UAUUG...GAG-3'\n______________GAUC_GAUGGAGGGG___GGGAAU_GAGA_GUA\n______________||||_|||:||||||___|||||:_||||_|||\n______________CUAG_CUAUCUCCCC___CCCUUG_CUCU_CAU\n3'-UUU...UAGAA____A__________AAC______U________CGAAG...AUA-5'\n_____________|_________________________________|\n_____________45________________________________12\n |
183 -- 189 |
30 -- 36 |
-39.4543 kcal/mol |
8.17177 kcal/mol |
9.94171 kcal/mol |
174 206@LB@ | |@LB@5'-UAU...UAAAC G GA U UAUUG...GAG-3'@LB@ GAUC GAUGGAGGGG GGGAAU GAGA GUA@LB@ |||| |||:|||||| |||||: |||| |||@LB@ CUAG CUAUCUCCCC CCCUUG CUCU CAU@LB@3'-UUU...UAGAA A AAC U CGAAG...AUA-5'@LB@ | |@LB@ 45 12@LB@@LB@@LB@Energy -21.34 kcal/mol@LB@Hybridization Energy -39.4543 kcal/mol@LB@Unfolding Energy - mRNA 8.17177 kcal/mol@LB@Unfolding Energy - sRNA 9.94171 kcal/mol@LB@@LB@Position - mRNA 175 -- 205@LB@Position - sRNA 13 -- 44@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 106 |
0.009109 |
0.2778 |
bsu01390 |
infA |
-12.86 |
0.075307 |
175 -- 189 |
30 -- 45 |
translation initiation factor IF-1 |
|
_____________174______________190\n_____________|________________|\n5'-CCA...GUUAC____________A___AGAAC...UGG-3'\n______________UGAUUUGA_AGA_GGG\n______________||||:|||_|||_|||\n______________ACUAGACU_UCU_CCC\n3'-UUU...CUAGA________A___C___AACCC...AUA-5'\n_____________|________________|\n_____________46_______________29\n |
176 -- 182 |
38 -- 44 |
-16.3452 kcal/mol |
2.51395 kcal/mol |
0.969561 kcal/mol |
174 190@LB@ | |@LB@5'-CCA...GUUAC A AGAAC...UGG-3'@LB@ UGAUUUGA AGA GGG@LB@ ||||:||| ||| |||@LB@ ACUAGACU UCU CCC@LB@3'-UUU...CUAGA A C AACCC...AUA-5'@LB@ | |@LB@ 46 29@LB@@LB@@LB@Energy -12.86 kcal/mol@LB@Hybridization Energy -16.3452 kcal/mol@LB@Unfolding Energy - mRNA 2.51395 kcal/mol@LB@Unfolding Energy - sRNA 0.969561 kcal/mol@LB@@LB@Position - mRNA 175 -- 189@LB@Position - sRNA 30 -- 45@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 38 -- 44@LB@ |
