| 82 |
0.006245 |
0.254 |
bsu12400 |
yjnA |
-14.90 |
0.031572 |
235 -- 273 |
19 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________234_____________________________________274\n_____________|_______________________________________|\n5'-UUA...GUUUG_____GC___A___G___U__C___A__G__U_______CCUUA...UGC-3'\n______________UCGGA__CUU_GUC_GAU_GA_GGG_GU_GG_GGAGCGG\n______________|||||__|||_:||_|||_||_|||_||_||_|||:|:|\n______________AGCCU__GAA_UAG_CUA_CU_CCC_CA_CC_CCUUGUC\n3'-UUU...AAAAA_____A____C___A___U_________A__________UCUCA...AUA-5'\n_____________|_______________________________________|\n_____________54______________________________________18\n |
267 -- 273 |
19 -- 25 |
-33.8463 kcal/mol |
12.0212 kcal/mol |
6.92635 kcal/mol |
234 274@LB@ | |@LB@5'-UUA...GUUUG GC A G U C A G U CCUUA...UGC-3'@LB@ UCGGA CUU GUC GAU GA GGG GU GG GGAGCGG@LB@ ||||| ||| :|| ||| || ||| || || |||:|:|@LB@ AGCCU GAA UAG CUA CU CCC CA CC CCUUGUC@LB@3'-UUU...AAAAA A C A U A UCUCA...AUA-5'@LB@ | |@LB@ 54 18@LB@@LB@@LB@Energy -14.90 kcal/mol@LB@Hybridization Energy -33.8463 kcal/mol@LB@Unfolding Energy - mRNA 12.0212 kcal/mol@LB@Unfolding Energy - sRNA 6.92635 kcal/mol@LB@@LB@Position - mRNA 235 -- 273@LB@Position - sRNA 19 -- 53@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 32 |
0.001154 |
0.119 |
bsu32820 |
fadE |
-9.01 |
0.290043 |
236 -- 252 |
32 -- 52 |
acyl-CoA dehydrogenase |
|
_____________235____________________253\n_____________|______________________|\n5'-AUU...GGCGG________UA____________AUGUC...AGC-3'\n______________CGGAUUUU__AUC_____GAGG\n______________|||||:||__|||_____||||\n______________GCCUAGAA__UAG_____CUCC\n3'-UUU...AAAAA________C____ACUAU____CCAAC...AUA-5'\n_____________|______________________|\n_____________53_____________________31\n |
237 -- 243 |
45 -- 51 |
-13.909 kcal/mol |
3.91995 kcal/mol |
0.976384 kcal/mol |
235 253@LB@ | |@LB@5'-AUU...GGCGG UA AUGUC...AGC-3'@LB@ CGGAUUUU AUC GAGG@LB@ |||||:|| ||| ||||@LB@ GCCUAGAA UAG CUCC@LB@3'-UUU...AAAAA C ACUAU CCAAC...AUA-5'@LB@ | |@LB@ 53 31@LB@@LB@@LB@Energy -9.01 kcal/mol@LB@Hybridization Energy -13.909 kcal/mol@LB@Unfolding Energy - mRNA 3.91995 kcal/mol@LB@Unfolding Energy - sRNA 0.976384 kcal/mol@LB@@LB@Position - mRNA 236 -- 252@LB@Position - sRNA 32 -- 52@LB@Position Seed - mRNA 237 -- 243@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 92 |
0.007313 |
0.2652 |
bsu34470 |
yveA |
-16.72 |
0.013215 |
240 -- 265 |
25 -- 47 |
aspartate-proton symporter |
|
_____________239________________________266\n_____________|__________________________|\n5'-CCU...CGCUG___________UUU__UU___A____AGCCA...UCA-3'\n______________UUUGAUCUGAU___GA__GGG_UGGG\n______________:||||||||||___||__|||_||||\n______________GAACUAGACUA___CU__CCC_ACCC\n3'-UUU...GCCUA___________U____C____A____CUUGU...AUA-5'\n_____________|__________________________|\n_____________48_________________________24\n |
244 -- 250 |
37 -- 43 |
-24.6158 kcal/mol |
6.92071 kcal/mol |
0.975871 kcal/mol |
239 266@LB@ | |@LB@5'-CCU...CGCUG UUU UU A AGCCA...UCA-3'@LB@ UUUGAUCUGAU GA GGG UGGG@LB@ :|||||||||| || ||| ||||@LB@ GAACUAGACUA CU CCC ACCC@LB@3'-UUU...GCCUA U C A CUUGU...AUA-5'@LB@ | |@LB@ 48 24@LB@@LB@@LB@Energy -16.72 kcal/mol@LB@Hybridization Energy -24.6158 kcal/mol@LB@Unfolding Energy - mRNA 6.92071 kcal/mol@LB@Unfolding Energy - sRNA 0.975871 kcal/mol@LB@@LB@Position - mRNA 240 -- 265@LB@Position - sRNA 25 -- 47@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 159 |
0.01439 |
0.3008 |
bsu00560 |
spoVT |
-10.85 |
0.160189 |
242 -- 254 |
41 -- 53 |
stage V sporulation protein T |
|
_____________241___________255\n_____________|_____________|\n5'-ACA...UUAGG_______G_____CCUAA...CGC-3'\n______________UCGGGUC_UGAUU\n______________||||:||_||||:\n______________AGCCUAG_ACUAG\n3'-UUU...AAAAA_______A_____ACUAU...AUA-5'\n_____________|_____________|\n_____________54____________40\n |
242 -- 248 |
47 -- 53 |
-14.0147 kcal/mol |
2.18551 kcal/mol |
0.979862 kcal/mol |
241 255@LB@ | |@LB@5'-ACA...UUAGG G CCUAA...CGC-3'@LB@ UCGGGUC UGAUU@LB@ ||||:|| ||||:@LB@ AGCCUAG ACUAG@LB@3'-UUU...AAAAA A ACUAU...AUA-5'@LB@ | |@LB@ 54 40@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -14.0147 kcal/mol@LB@Unfolding Energy - mRNA 2.18551 kcal/mol@LB@Unfolding Energy - sRNA 0.979862 kcal/mol@LB@@LB@Position - mRNA 242 -- 254@LB@Position - sRNA 41 -- 53@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 71 |
0.005061 |
0.2378 |
bsu02170 |
ybfB |
-12.87 |
0.075045 |
244 -- 269 |
24 -- 48 |
MFS transporter |
bsu03530 |
_____________243__________________________270\n_____________|____________________________|\n5'-CUU...CACCU_____________UU__C____CA____GUGCG...AGC-3'\n______________UUUUGAUCU__UA__GG_GGUU__GGGG\n______________|:|||||||__||__||_||||__||||\n______________AGAACUAGA__AU__CC_CCAA__CCCC\n3'-UUU...AGCCU_________CU__CU_____________UUGUC...AUA-5'\n_____________|____________________________|\n_____________49___________________________23\n |
246 -- 252 |
40 -- 46 |
-21.3597 kcal/mol |
7.5105 kcal/mol |
0.978761 kcal/mol |
243 270@LB@ | |@LB@5'-CUU...CACCU UU C CA GUGCG...AGC-3'@LB@ UUUUGAUCU UA GG GGUU GGGG@LB@ |:||||||| || || |||| ||||@LB@ AGAACUAGA AU CC CCAA CCCC@LB@3'-UUU...AGCCU CU CU UUGUC...AUA-5'@LB@ | |@LB@ 49 23@LB@@LB@@LB@Energy -12.87 kcal/mol@LB@Hybridization Energy -21.3597 kcal/mol@LB@Unfolding Energy - mRNA 7.5105 kcal/mol@LB@Unfolding Energy - sRNA 0.978761 kcal/mol@LB@@LB@Position - mRNA 244 -- 269@LB@Position - sRNA 24 -- 48@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 56 |
0.002918 |
0.1738 |
bsu04390 |
ydbA |
-13.95 |
0.047884 |
245 -- 258 |
25 -- 39 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________244_____________259\n_____________|_______________|\n5'-UUC...AAAGA_______AAA_____AAAAA...GCC-3'\n______________GAUAGAG____UGGG\n______________|||||||____||||\n______________CUAUCUC____ACCC\n3'-UUU...CUAGA_______CCCA____CUUGU...AUA-5'\n_____________|_______________|\n_____________40______________24\n |
245 -- 251 |
33 -- 39 |
-15.533 kcal/mol |
0.795684 kcal/mol |
0.783603 kcal/mol |
244 259@LB@ | |@LB@5'-UUC...AAAGA AAA AAAAA...GCC-3'@LB@ GAUAGAG UGGG@LB@ ||||||| ||||@LB@ CUAUCUC ACCC@LB@3'-UUU...CUAGA CCCA CUUGU...AUA-5'@LB@ | |@LB@ 40 24@LB@@LB@@LB@Energy -13.95 kcal/mol@LB@Hybridization Energy -15.533 kcal/mol@LB@Unfolding Energy - mRNA 0.795684 kcal/mol@LB@Unfolding Energy - sRNA 0.783603 kcal/mol@LB@@LB@Position - mRNA 245 -- 258@LB@Position - sRNA 25 -- 39@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 91 |
0.007102 |
0.2604 |
bsu36520 |
glnK |
-7.98 |
0.387566 |
247 -- 267 |
32 -- 49 |
nitrogen regulatory PII-like protein |
|
_____________246____________________268\n_____________|______________________|\n5'-GGU...GGCAA_______AAAG_______C___AACUC...CUU-3'\n______________AUUUUGA____CUGA_AG_AGG\n______________||:||||____||||_||_|||\n______________UAGAACU____GACU_UC_UCC\n3'-UUU...AAGCC_______A_______A______CCAAC...AUA-5'\n_____________|______________________|\n_____________50_____________________31\n |
247 -- 253 |
43 -- 49 |
-11.0905 kcal/mol |
2.14263 kcal/mol |
0.970192 kcal/mol |
246 268@LB@ | |@LB@5'-GGU...GGCAA AAAG C AACUC...CUU-3'@LB@ AUUUUGA CUGA AG AGG@LB@ ||:|||| |||| || |||@LB@ UAGAACU GACU UC UCC@LB@3'-UUU...AAGCC A A CCAAC...AUA-5'@LB@ | |@LB@ 50 31@LB@@LB@@LB@Energy -7.98 kcal/mol@LB@Hybridization Energy -11.0905 kcal/mol@LB@Unfolding Energy - mRNA 2.14263 kcal/mol@LB@Unfolding Energy - sRNA 0.970192 kcal/mol@LB@@LB@Position - mRNA 247 -- 267@LB@Position - sRNA 32 -- 49@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 43 -- 49@LB@ |
| 128 |
0.01143 |
0.2883 |
bsu29690 |
acuA |
-15.81 |
0.020690 |
248 -- 269 |
30 -- 52 |
acetoin utilization protein AcuA |
|
_____________247_____________________270\n_____________|_______________________|\n5'-UAU...GCAAC___C____UA_________A___CCUGU...AUG-3'\n______________CGG_UCUU___CUGAUAGA_GGG\n______________|||_||||___||||||||_|||\n______________GCC_AGAA___GACUAUCU_CCC\n3'-UUU...AAAAA___U____CUA________C___AACCC...AUA-5'\n_____________|_______________________|\n_____________53______________________29\n |
259 -- 265 |
34 -- 40 |
-22.8929 kcal/mol |
6.10629 kcal/mol |
0.979974 kcal/mol |
247 270@LB@ | |@LB@5'-UAU...GCAAC C UA A CCUGU...AUG-3'@LB@ CGG UCUU CUGAUAGA GGG@LB@ ||| |||| |||||||| |||@LB@ GCC AGAA GACUAUCU CCC@LB@3'-UUU...AAAAA U CUA C AACCC...AUA-5'@LB@ | |@LB@ 53 29@LB@@LB@@LB@Energy -15.81 kcal/mol@LB@Hybridization Energy -22.8929 kcal/mol@LB@Unfolding Energy - mRNA 6.10629 kcal/mol@LB@Unfolding Energy - sRNA 0.979974 kcal/mol@LB@@LB@Position - mRNA 248 -- 269@LB@Position - sRNA 30 -- 52@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 123 |
0.01093 |
0.2883 |
bsu22970 |
ypbH |
-15.60 |
0.022809 |
254 -- 275 |
27 -- 45 |
adapter protein MecA |
|
_____________253____________________276\n_____________|______________________|\n5'-GAG...CUCGA________C___CC____A___ACAAA...ACU-3'\n______________UGAUUUGA_AGA__GGGG_UUG\n______________||||:|||_|||__||||_|||\n______________ACUAGACU_UCU__CCCC_AAC\n3'-UUU...CUAGA________A_____________CCCUU...AUA-5'\n_____________|______________________|\n_____________46_____________________26\n |
255 -- 261 |
38 -- 44 |
-20.0369 kcal/mol |
3.46393 kcal/mol |
0.971562 kcal/mol |
253 276@LB@ | |@LB@5'-GAG...CUCGA C CC A ACAAA...ACU-3'@LB@ UGAUUUGA AGA GGGG UUG@LB@ ||||:||| ||| |||| |||@LB@ ACUAGACU UCU CCCC AAC@LB@3'-UUU...CUAGA A CCCUU...AUA-5'@LB@ | |@LB@ 46 26@LB@@LB@@LB@Energy -15.60 kcal/mol@LB@Hybridization Energy -20.0369 kcal/mol@LB@Unfolding Energy - mRNA 3.46393 kcal/mol@LB@Unfolding Energy - sRNA 0.971562 kcal/mol@LB@@LB@Position - mRNA 254 -- 275@LB@Position - sRNA 27 -- 45@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 35 |
0.001389 |
0.1324 |
bsu24570 |
gcvT |
-12.27 |
0.095214 |
256 -- 273 |
25 -- 46 |
aminomethyltransferase |
|
_____________255____________________274\n_____________|______________________|\n5'-CUC...AACGA_______C_______C______AGCUU...UAA-3'\n______________UUGAUUU___GGAGG___UGGG\n______________|||||:|___:||||___||||\n______________AACUAGA___UCUCC___ACCC\n3'-UUU...CCUAG_______CUA_____CCA____CUUGU...AUA-5'\n_____________|______________________|\n_____________47_____________________24\n |
256 -- 262 |
40 -- 46 |
-17.62 kcal/mol |
4.38211 kcal/mol |
0.972727 kcal/mol |
255 274@LB@ | |@LB@5'-CUC...AACGA C C AGCUU...UAA-3'@LB@ UUGAUUU GGAGG UGGG@LB@ |||||:| :|||| ||||@LB@ AACUAGA UCUCC ACCC@LB@3'-UUU...CCUAG CUA CCA CUUGU...AUA-5'@LB@ | |@LB@ 47 24@LB@@LB@@LB@Energy -12.27 kcal/mol@LB@Hybridization Energy -17.62 kcal/mol@LB@Unfolding Energy - mRNA 4.38211 kcal/mol@LB@Unfolding Energy - sRNA 0.972727 kcal/mol@LB@@LB@Position - mRNA 256 -- 273@LB@Position - sRNA 25 -- 46@LB@Position Seed - mRNA 256 -- 262@LB@Position Seed - sRNA 40 -- 46@LB@ |
