| 156 |
0.0141 |
0.3008 |
bsu23600 |
yqxK |
-15.33 |
0.025853 |
66 -- 91 |
32 -- 51 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________65_________________________92\n_____________|__________________________|\n5'-GAA...GAUGA___CGAGUU_G____AG_________UGACG...GAA-3'\n______________GGA______U_UUGA__UGAUGGAGG\n______________|||______|_||||__||||:||||\n______________CCU______A_AACU__ACUAUCUCC\n3'-UUU...AAAAG__________G____AG_________CCAAC...AUA-5'\n_____________|__________________________|\n_____________52_________________________31\n |
85 -- 91 |
32 -- 38 |
-19.9693 kcal/mol |
3.66503 kcal/mol |
0.97021 kcal/mol |
65 92@LB@ | |@LB@5'-GAA...GAUGA CGAGUU G AG UGACG...GAA-3'@LB@ GGA U UUGA UGAUGGAGG@LB@ ||| | |||| ||||:||||@LB@ CCU A AACU ACUAUCUCC@LB@3'-UUU...AAAAG G AG CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -15.33 kcal/mol@LB@Hybridization Energy -19.9693 kcal/mol@LB@Unfolding Energy - mRNA 3.66503 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 66 -- 91@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 95 |
0.007929 |
0.2778 |
bsu40080 |
gntZ |
-7.93 |
0.392252 |
74 -- 93 |
34 -- 54 |
6-phosphogluconate dehydrogenase |
|
_____________73____________________94\n_____________|_____________________|\n5'-ACU...AAUAC________AAGCA____A___AACAU...ACA-3'\n______________UUCGGAUU______UGA_AGA\n______________|||||||:______|||_|||\n______________AAGCCUAG______ACU_UCU\n3'-UUU...CAAAA________AACUAG___A___CCCCA...AUA-5'\n_____________|_____________________|\n_____________55____________________33\n |
75 -- 81 |
47 -- 53 |
-13.3211 kcal/mol |
3.78785 kcal/mol |
1.60142 kcal/mol |
73 94@LB@ | |@LB@5'-ACU...AAUAC AAGCA A AACAU...ACA-3'@LB@ UUCGGAUU UGA AGA@LB@ |||||||: ||| |||@LB@ AAGCCUAG ACU UCU@LB@3'-UUU...CAAAA AACUAG A CCCCA...AUA-5'@LB@ | |@LB@ 55 33@LB@@LB@@LB@Energy -7.93 kcal/mol@LB@Hybridization Energy -13.3211 kcal/mol@LB@Unfolding Energy - mRNA 3.78785 kcal/mol@LB@Unfolding Energy - sRNA 1.60142 kcal/mol@LB@@LB@Position - mRNA 74 -- 93@LB@Position - sRNA 34 -- 54@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 101 |
0.008451 |
0.2778 |
bsu32150 |
paiA |
-11.07 |
0.148459 |
77 -- 92 |
23 -- 39 |
protease synthase and sporulation negative regulatory protein PAI 1 |
|
_____________76________________93\n_____________|_________________|\n5'-AUU...AUCAC_______A______U__UGAAG...CAC-3'\n______________GAUAGGG__GUUGG_GA\n______________|||||:|__|||||_||\n______________CUAUCUC__CAACC_CU\n3'-UUU...CUAGA_______CC_____C__UGUCU...AUA-5'\n_____________|_________________|\n_____________40________________22\n |
77 -- 83 |
33 -- 39 |
-14.8457 kcal/mol |
2.98855 kcal/mol |
0.791731 kcal/mol |
76 93@LB@ | |@LB@5'-AUU...AUCAC A U UGAAG...CAC-3'@LB@ GAUAGGG GUUGG GA@LB@ |||||:| ||||| ||@LB@ CUAUCUC CAACC CU@LB@3'-UUU...CUAGA CC C UGUCU...AUA-5'@LB@ | |@LB@ 40 22@LB@@LB@@LB@Energy -11.07 kcal/mol@LB@Hybridization Energy -14.8457 kcal/mol@LB@Unfolding Energy - mRNA 2.98855 kcal/mol@LB@Unfolding Energy - sRNA 0.791731 kcal/mol@LB@@LB@Position - mRNA 77 -- 92@LB@Position - sRNA 23 -- 39@LB@Position Seed - mRNA 77 -- 83@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 107 |
0.009163 |
0.2778 |
bsu12780 |
xepA |
-15.86 |
0.020153 |
79 -- 99 |
29 -- 48 |
phage-like element PBSX protein XepA |
|
_____________78____________________100\n_____________|_____________________|\n5'-UUG...UCAAU__AG_______C______U__GAACA...UUU-3'\n______________UC__GAUCUGG_AGAGGG_GU\n______________||__||||||:_||||||_||\n______________AG__CUAGACU_UCUCCC_CA\n3'-UUU...AGCCU__AA_______A_________ACCCC...AUA-5'\n_____________|_____________________|\n_____________49____________________28\n |
83 -- 89 |
38 -- 44 |
-22.9588 kcal/mol |
6.12286 kcal/mol |
0.974361 kcal/mol |
78 100@LB@ | |@LB@5'-UUG...UCAAU AG C U GAACA...UUU-3'@LB@ UC GAUCUGG AGAGGG GU@LB@ || ||||||: |||||| ||@LB@ AG CUAGACU UCUCCC CA@LB@3'-UUU...AGCCU AA A ACCCC...AUA-5'@LB@ | |@LB@ 49 28@LB@@LB@@LB@Energy -15.86 kcal/mol@LB@Hybridization Energy -22.9588 kcal/mol@LB@Unfolding Energy - mRNA 6.12286 kcal/mol@LB@Unfolding Energy - sRNA 0.974361 kcal/mol@LB@@LB@Position - mRNA 79 -- 99@LB@Position - sRNA 29 -- 48@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 168 |
0.01631 |
0.3152 |
bsu14170 |
ykuP |
-12.43 |
0.089417 |
80 -- 100 |
11 -- 27 |
flavodoxin-2 |
|
_____________79____________________101\n_____________|_____________________|\n5'-CAC...AUUAA___ACAU______________CAAUC...UAG-3'\n______________GGG____GGAUGGAGAGUGGC\n______________|||____|:|::||||||:||\n______________CCC____CUUGUCUCUCAUCG\n3'-UUU...CCCAA_____________________AAGAG...AUA-5'\n_____________|_____________________|\n_____________28____________________10\n |
94 -- 100 |
11 -- 17 |
-24.7716 kcal/mol |
6.31596 kcal/mol |
6.0284 kcal/mol |
79 101@LB@ | |@LB@5'-CAC...AUUAA ACAU CAAUC...UAG-3'@LB@ GGG GGAUGGAGAGUGGC@LB@ ||| |:|::||||||:||@LB@ CCC CUUGUCUCUCAUCG@LB@3'-UUU...CCCAA AAGAG...AUA-5'@LB@ | |@LB@ 28 10@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -24.7716 kcal/mol@LB@Unfolding Energy - mRNA 6.31596 kcal/mol@LB@Unfolding Energy - sRNA 6.0284 kcal/mol@LB@@LB@Position - mRNA 80 -- 100@LB@Position - sRNA 11 -- 27@LB@Position Seed - mRNA 94 -- 100@LB@Position Seed - sRNA 11 -- 17@LB@ |
| 192 |
0.01921 |
0.3269 |
bsu12649 |
ykzM |
-11.69 |
0.118448 |
82 -- 103 |
11 -- 32 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu26089 |
_____________81______________________104\n_____________|_______________________|\n5'-AAG...GACAA____A____________C_A___GGCCA...UCG-3'\n______________GGGU__GGGGACAGGGG_G_GGC\n______________||||__|||:||||:|:_|_:||\n______________CCCA__CCCUUGUCUCU_C_UCG\n3'-UUU...AUCUC____AC_____________A___AAGAG...AUA-5'\n_____________|_______________________|\n_____________33______________________10\n |
88 -- 94 |
19 -- 25 |
-26.088 kcal/mol |
7.89329 kcal/mol |
6.50677 kcal/mol |
81 104@LB@ | |@LB@5'-AAG...GACAA A C A GGCCA...UCG-3'@LB@ GGGU GGGGACAGGGG G GGC@LB@ |||| |||:||||:|: | :||@LB@ CCCA CCCUUGUCUCU C UCG@LB@3'-UUU...AUCUC AC A AAGAG...AUA-5'@LB@ | |@LB@ 33 10@LB@@LB@@LB@Energy -11.69 kcal/mol@LB@Hybridization Energy -26.088 kcal/mol@LB@Unfolding Energy - mRNA 7.89329 kcal/mol@LB@Unfolding Energy - sRNA 6.50677 kcal/mol@LB@@LB@Position - mRNA 82 -- 103@LB@Position - sRNA 11 -- 32@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 77 |
0.005688 |
0.2441 |
bsu35180 |
csbA |
-19.97 |
0.002143 |
87 -- 126 |
11 -- 50 |
general stress protein CsbA |
|
_____________86__________________________________________127\n_____________|___________________________________________|\n5'-UGA...UGAUU_____________AA_______A___U_U__G___________GAAUG...CAG-3'\n______________GAUUUUGG_CUGA___AGGGGU_UGG_G_AA_UAGAGGG_AGC\n______________|||:|||:_||||___||||||_|||_|_||_:||||:|_|||\n______________CUAGAACU_GACU___UCCCCA_ACC_C_UU_GUCUCUC_UCG\n3'-UUU...AAAGC________A____AUC____________C__________A___AAGAG...AUA-5'\n_____________|___________________________________________|\n_____________51__________________________________________10\n |
117 -- 123 |
15 -- 21 |
-37.9995 kcal/mol |
8.07502 kcal/mol |
9.95602 kcal/mol |
86 127@LB@ | |@LB@5'-UGA...UGAUU AA A U U G GAAUG...CAG-3'@LB@ GAUUUUGG CUGA AGGGGU UGG G AA UAGAGGG AGC@LB@ |||:|||: |||| |||||| ||| | || :||||:| |||@LB@ CUAGAACU GACU UCCCCA ACC C UU GUCUCUC UCG@LB@3'-UUU...AAAGC A AUC C A AAGAG...AUA-5'@LB@ | |@LB@ 51 10@LB@@LB@@LB@Energy -19.97 kcal/mol@LB@Hybridization Energy -37.9995 kcal/mol@LB@Unfolding Energy - mRNA 8.07502 kcal/mol@LB@Unfolding Energy - sRNA 9.95602 kcal/mol@LB@@LB@Position - mRNA 87 -- 126@LB@Position - sRNA 11 -- 50@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 80 |
0.005932 |
0.2448 |
bsu24430 |
spoIIIAA |
-11.72 |
0.117179 |
88 -- 116 |
28 -- 52 |
stage III sporulation protein AA |
|
_____________87______________________________117\n_____________|_______________________________|\n5'-GUC...UUUUA___GUGUU_______________C___U___CAUAA...GGA-3'\n______________CGG_____GUCUUGAUUU__UGG_GGG_GUU\n______________|||_____:|||||||:|__|:|_|||_|||\n______________GCC_____UAGAACUAGA__AUC_CCC_CAA\n3'-UUU...AAAAA__________________CU___U_______CCCCU...AUA-5'\n_____________|_______________________________|\n_____________53______________________________27\n |
99 -- 105 |
40 -- 46 |
-20.9874 kcal/mol |
8.2899 kcal/mol |
0.980555 kcal/mol |
87 117@LB@ | |@LB@5'-GUC...UUUUA GUGUU C U CAUAA...GGA-3'@LB@ CGG GUCUUGAUUU UGG GGG GUU@LB@ ||| :|||||||:| |:| ||| |||@LB@ GCC UAGAACUAGA AUC CCC CAA@LB@3'-UUU...AAAAA CU U CCCCU...AUA-5'@LB@ | |@LB@ 53 27@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -20.9874 kcal/mol@LB@Unfolding Energy - mRNA 8.2899 kcal/mol@LB@Unfolding Energy - sRNA 0.980555 kcal/mol@LB@@LB@Position - mRNA 88 -- 116@LB@Position - sRNA 28 -- 52@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 153 |
0.01377 |
0.2994 |
bsu37510 |
pbpG |
-15.25 |
0.026855 |
95 -- 111 |
23 -- 38 |
penicillin-binding protein 2D |
|
_____________94_________________112\n_____________|__________________|\n5'-UGU...AAAGU_______AU__C______AAAAG...UGU-3'\n______________AUAGAGG__GG__GGGGA\n______________|||||||__||__|||||\n______________UAUCUCC__CC__CCCCU\n3'-UUU...UAGAC___________AA_____UGUCU...AUA-5'\n_____________|__________________|\n_____________39_________________22\n |
95 -- 101 |
32 -- 38 |
-18.2895 kcal/mol |
2.3676 kcal/mol |
0.66977 kcal/mol |
94 112@LB@ | |@LB@5'-UGU...AAAGU AU C AAAAG...UGU-3'@LB@ AUAGAGG GG GGGGA@LB@ ||||||| || |||||@LB@ UAUCUCC CC CCCCU@LB@3'-UUU...UAGAC AA UGUCU...AUA-5'@LB@ | |@LB@ 39 22@LB@@LB@@LB@Energy -15.25 kcal/mol@LB@Hybridization Energy -18.2895 kcal/mol@LB@Unfolding Energy - mRNA 2.3676 kcal/mol@LB@Unfolding Energy - sRNA 0.66977 kcal/mol@LB@@LB@Position - mRNA 95 -- 111@LB@Position - sRNA 23 -- 38@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 78 |
0.005707 |
0.2441 |
bsu18040 |
yneP |
-11.92 |
0.108570 |
96 -- 119 |
24 -- 45 |
acyl-CoA thioesterase |
|
_____________95_______________________120\n_____________|________________________|\n5'-UUG...CUUUC_________UAU__UAAAA_____CUAAA...AAA-3'\n______________UGAUUUGAU___GG_____UGGGG\n______________||||:||||___||_____|||||\n______________ACUAGACUA___CC_____ACCCC\n3'-UUU...CUAGA_________UCU__CCA_______UUGUC...AUA-5'\n_____________|________________________|\n_____________46_______________________23\n |
98 -- 104 |
37 -- 43 |
-18.893 kcal/mol |
5.99443 kcal/mol |
0.977634 kcal/mol |
95 120@LB@ | |@LB@5'-UUG...CUUUC UAU UAAAA CUAAA...AAA-3'@LB@ UGAUUUGAU GG UGGGG@LB@ ||||:|||| || |||||@LB@ ACUAGACUA CC ACCCC@LB@3'-UUU...CUAGA UCU CCA UUGUC...AUA-5'@LB@ | |@LB@ 46 23@LB@@LB@@LB@Energy -11.92 kcal/mol@LB@Hybridization Energy -18.893 kcal/mol@LB@Unfolding Energy - mRNA 5.99443 kcal/mol@LB@Unfolding Energy - sRNA 0.977634 kcal/mol@LB@@LB@Position - mRNA 96 -- 119@LB@Position - sRNA 24 -- 45@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 37 -- 43@LB@ |
