| 197 |
0.0195 |
0.3269 |
bsu04670 |
rsbRA |
-10.67 |
0.170227 |
19 -- 37 |
34 -- 52 |
RsbT co-antagonist protein RsbRA |
|
_____________18__________________38\n_____________|___________________|\n5'-CGG...UCAUC_____GA___AA_______UAAGG...AAU-3'\n______________UGGAU__UGA__UGAUGGA\n______________:||||__|||__||||:||\n______________GCCUA__ACU__ACUAUCU\n3'-UUU...AAAAA_____GA___AG_______CCCCA...AUA-5'\n_____________|___________________|\n_____________53__________________33\n |
31 -- 37 |
34 -- 40 |
-17.5102 kcal/mol |
5.86002 kcal/mol |
0.976339 kcal/mol |
18 38@LB@ | |@LB@5'-CGG...UCAUC GA AA UAAGG...AAU-3'@LB@ UGGAU UGA UGAUGGA@LB@ :|||| ||| ||||:||@LB@ GCCUA ACU ACUAUCU@LB@3'-UUU...AAAAA GA AG CCCCA...AUA-5'@LB@ | |@LB@ 53 33@LB@@LB@@LB@Energy -10.67 kcal/mol@LB@Hybridization Energy -17.5102 kcal/mol@LB@Unfolding Energy - mRNA 5.86002 kcal/mol@LB@Unfolding Energy - sRNA 0.976339 kcal/mol@LB@@LB@Position - mRNA 19 -- 37@LB@Position - sRNA 34 -- 52@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 64 |
0.004032 |
0.2071 |
bsu23050 |
fmnP |
-11.92 |
0.108419 |
30 -- 48 |
32 -- 51 |
riboflavin transporter FmnP |
|
_____________29___________________49\n_____________|____________________|\n5'-GUG...AGUCU____GG___GAA________UUCAU...UAA-3'\n______________GGAU___GA___GAUGGAGG\n______________||||___||___|||:||||\n______________CCUA___CU___CUAUCUCC\n3'-UUU...AAAAG____GAA__AGA________CCAAC...AUA-5'\n_____________|____________________|\n_____________52___________________31\n |
42 -- 48 |
32 -- 38 |
-18.1391 kcal/mol |
5.24431 kcal/mol |
0.97021 kcal/mol |
29 49@LB@ | |@LB@5'-GUG...AGUCU GG GAA UUCAU...UAA-3'@LB@ GGAU GA GAUGGAGG@LB@ |||| || |||:||||@LB@ CCUA CU CUAUCUCC@LB@3'-UUU...AAAAG GAA AGA CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -11.92 kcal/mol@LB@Hybridization Energy -18.1391 kcal/mol@LB@Unfolding Energy - mRNA 5.24431 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 30 -- 48@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 182 |
0.01724 |
0.3161 |
bsu22340 |
nth |
-14.82 |
0.032761 |
30 -- 59 |
25 -- 52 |
endonuclease III |
|
_____________29______________________________60\n_____________|_______________________________|\n5'-CAG...UUGAC______________A_____AGAAAAA____CUUAA...UAG-3'\n______________CGGAUUUUG_UUUG_AUGGA_______UGGG\n______________|||||:|||_|:||_||:||_______||||\n______________GCCUAGAAC_AGAC_UAUCU_______ACCC\n3'-UUU...AAAAA_________U__________CCCCA______CUUGU...AUA-5'\n_____________|_______________________________|\n_____________53______________________________24\n |
32 -- 38 |
44 -- 50 |
-22.2299 kcal/mol |
6.43208 kcal/mol |
0.980838 kcal/mol |
29 60@LB@ | |@LB@5'-CAG...UUGAC A AGAAAAA CUUAA...UAG-3'@LB@ CGGAUUUUG UUUG AUGGA UGGG@LB@ |||||:||| |:|| ||:|| ||||@LB@ GCCUAGAAC AGAC UAUCU ACCC@LB@3'-UUU...AAAAA U CCCCA CUUGU...AUA-5'@LB@ | |@LB@ 53 24@LB@@LB@@LB@Energy -14.82 kcal/mol@LB@Hybridization Energy -22.2299 kcal/mol@LB@Unfolding Energy - mRNA 6.43208 kcal/mol@LB@Unfolding Energy - sRNA 0.980838 kcal/mol@LB@@LB@Position - mRNA 30 -- 59@LB@Position - sRNA 25 -- 52@LB@Position Seed - mRNA 32 -- 38@LB@Position Seed - sRNA 44 -- 50@LB@ |
| 140 |
0.01224 |
0.2883 |
bsu36480 |
ywoD |
-6.33 |
0.569704 |
31 -- 37 |
66 -- 72 |
MFS transporter |
|
_____________30______38\n_____________|_______|\n5'-AUU...AUAUC_______AAAGC...AUA-3'\n______________GGGGUGG\n______________|||||:|\n______________CCCCAUC\n3'-UUU...ACCGC_______GCGGU...AUA-5'\n_____________|_______|\n_____________73______65\n |
31 -- 37 |
66 -- 72 |
-11.1749 kcal/mol |
4.83937 kcal/mol |
0.00366414 kcal/mol |
30 38@LB@ | |@LB@5'-AUU...AUAUC AAAGC...AUA-3'@LB@ GGGGUGG@LB@ |||||:|@LB@ CCCCAUC@LB@3'-UUU...ACCGC GCGGU...AUA-5'@LB@ | |@LB@ 73 65@LB@@LB@@LB@Energy -6.33 kcal/mol@LB@Hybridization Energy -11.1749 kcal/mol@LB@Unfolding Energy - mRNA 4.83937 kcal/mol@LB@Unfolding Energy - sRNA 0.00366414 kcal/mol@LB@@LB@Position - mRNA 31 -- 37@LB@Position - sRNA 66 -- 72@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 162 |
0.01461 |
0.3008 |
bsu28700 |
ysfE |
-18.20 |
0.006050 |
31 -- 59 |
26 -- 51 |
Evidence 5: No homology to any previously reported sequences |
bsu26940 |
_____________30____________________________60\n_____________|_____________________________|\n5'-AAG...UAGAA___CUA____AAA__CUG___________CAAUG...AAA-3'\n______________GGA___UUGA___UG___AGAGGGGUUGG\n______________|||___||||___||___|||||||||||\n______________CCU___AACU___AC___UCUCCCCAACC\n3'-UUU...AAAAG___AG_____AG___UA____________CCUUG...AUA-5'\n_____________|_____________________________|\n_____________52____________________________25\n |
53 -- 59 |
26 -- 32 |
-27.3955 kcal/mol |
8.22133 kcal/mol |
0.975822 kcal/mol |
30 60@LB@ | |@LB@5'-AAG...UAGAA CUA AAA CUG CAAUG...AAA-3'@LB@ GGA UUGA UG AGAGGGGUUGG@LB@ ||| |||| || |||||||||||@LB@ CCU AACU AC UCUCCCCAACC@LB@3'-UUU...AAAAG AG AG UA CCUUG...AUA-5'@LB@ | |@LB@ 52 25@LB@@LB@@LB@Energy -18.20 kcal/mol@LB@Hybridization Energy -27.3955 kcal/mol@LB@Unfolding Energy - mRNA 8.22133 kcal/mol@LB@Unfolding Energy - sRNA 0.975822 kcal/mol@LB@@LB@Position - mRNA 31 -- 59@LB@Position - sRNA 26 -- 51@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 26 -- 32@LB@ |
| 69 |
0.004753 |
0.2298 |
bsu24850 |
glcK |
-6.97 |
0.496286 |
33 -- 46 |
15 -- 29 |
glucokinase |
|
_____________32______________47\n_____________|_______________|\n5'-GCU...UUAUU____A__________CUUGA...UUC-3'\n______________UUGG__GACAGAGAG\n______________||||__:||||||||\n______________AACC__UUGUCUCUC\n3'-UUU...UCCCC____CC_________AUCGA...AUA-5'\n_____________|_______________|\n_____________30______________14\n |
40 -- 46 |
15 -- 21 |
-16.6304 kcal/mol |
3.64593 kcal/mol |
6.01316 kcal/mol |
32 47@LB@ | |@LB@5'-GCU...UUAUU A CUUGA...UUC-3'@LB@ UUGG GACAGAGAG@LB@ |||| :||||||||@LB@ AACC UUGUCUCUC@LB@3'-UUU...UCCCC CC AUCGA...AUA-5'@LB@ | |@LB@ 30 14@LB@@LB@@LB@Energy -6.97 kcal/mol@LB@Hybridization Energy -16.6304 kcal/mol@LB@Unfolding Energy - mRNA 3.64593 kcal/mol@LB@Unfolding Energy - sRNA 6.01316 kcal/mol@LB@@LB@Position - mRNA 33 -- 46@LB@Position - sRNA 15 -- 29@LB@Position Seed - mRNA 40 -- 46@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 45 |
0.002015 |
0.1494 |
bsu34040 |
yvfW |
-14.27 |
0.041807 |
34 -- 68 |
10 -- 49 |
lactate utilization protein B |
|
_____________33_______________________________________69\n_____________|________________________________________|\n5'-GCG...AAAAA___GA___G_________AAAC__________________GAUCG...GUC-3'\n______________GUC___AU_UG_UGGAGG_____GGGAGCAGAG_GU_GCU\n______________:||___||_||_|:||||_____||||:|||||_||_|||\n______________UAG___UA_AC_AUCUCC_____CCCUUGUCUC_CA_CGA\n3'-UUU...AAGCC___AAC__G__U______CCAAC__________U__U___AGAGA...AUA-5'\n_____________|________________________________________|\n_____________50_______________________________________9\n |
55 -- 61 |
19 -- 25 |
-34.7578 kcal/mol |
10.5343 kcal/mol |
9.9558 kcal/mol |
33 69@LB@ | |@LB@5'-GCG...AAAAA GA G AAAC GAUCG...GUC-3'@LB@ GUC AU UG UGGAGG GGGAGCAGAG GU GCU@LB@ :|| || || |:|||| ||||:||||| || |||@LB@ UAG UA AC AUCUCC CCCUUGUCUC CA CGA@LB@3'-UUU...AAGCC AAC G U CCAAC U U AGAGA...AUA-5'@LB@ | |@LB@ 50 9@LB@@LB@@LB@Energy -14.27 kcal/mol@LB@Hybridization Energy -34.7578 kcal/mol@LB@Unfolding Energy - mRNA 10.5343 kcal/mol@LB@Unfolding Energy - sRNA 9.9558 kcal/mol@LB@@LB@Position - mRNA 34 -- 68@LB@Position - sRNA 10 -- 49@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 79 |
0.005838 |
0.2448 |
bsu25980 |
yqbT |
-11.66 |
0.119794 |
52 -- 65 |
32 -- 53 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type ps: structure' |
bsu12730 |
_____________51_____________________66\n_____________|______________________|\n5'-CGU...AUCCC______________________AAGCA...CGU-3'\n______________UCGG________CUGAUAGAGG\n______________||||________||||||||||\n______________AGCC________GACUAUCUCC\n3'-UUU...AAAAA____UAGAACUA__________CCAAC...AUA-5'\n_____________|______________________|\n_____________54_____________________31\n |
59 -- 65 |
32 -- 38 |
-19.4839 kcal/mol |
6.84464 kcal/mol |
0.981755 kcal/mol |
51 66@LB@ | |@LB@5'-CGU...AUCCC AAGCA...CGU-3'@LB@ UCGG CUGAUAGAGG@LB@ |||| ||||||||||@LB@ AGCC GACUAUCUCC@LB@3'-UUU...AAAAA UAGAACUA CCAAC...AUA-5'@LB@ | |@LB@ 54 31@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -19.4839 kcal/mol@LB@Unfolding Energy - mRNA 6.84464 kcal/mol@LB@Unfolding Energy - sRNA 0.981755 kcal/mol@LB@@LB@Position - mRNA 52 -- 65@LB@Position - sRNA 32 -- 53@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 151 |
0.01347 |
0.2966 |
bsu25570 |
comEC |
-9.44 |
0.254651 |
59 -- 89 |
9 -- 50 |
ComE operon protein 3 |
|
_____________58__________________________________________90\n_____________|___________________________________________|\n5'-CGC...GAAAU____G_________AA____________________AA_____UCAUU...UCU-3'\n______________GAUC_UUGAUCUGA______________AACAGAGA__AGCUU\n______________||||_|||||||||______________||||||||__|||||\n______________CUAG_AACUAGACU______________UUGUCUCU__UCGAA\n3'-UUU...AAAGC______________AUCUCCCCAACCCC________CA_____GAGAGAUA-5'\n_____________|___________________________________________|\n_____________51__________________________________________8\n |
76 -- 82 |
16 -- 22 |
-28.9501 kcal/mol |
9.55134 kcal/mol |
9.9565 kcal/mol |
58 90@LB@ | |@LB@5'-CGC...GAAAU G AA AA UCAUU...UCU-3'@LB@ GAUC UUGAUCUGA AACAGAGA AGCUU@LB@ |||| ||||||||| |||||||| |||||@LB@ CUAG AACUAGACU UUGUCUCU UCGAA@LB@3'-UUU...AAAGC AUCUCCCCAACCCC CA GAGAGAUA-5'@LB@ | |@LB@ 51 8@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -28.9501 kcal/mol@LB@Unfolding Energy - mRNA 9.55134 kcal/mol@LB@Unfolding Energy - sRNA 9.9565 kcal/mol@LB@@LB@Position - mRNA 59 -- 89@LB@Position - sRNA 9 -- 50@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 16 -- 22@LB@ |
| 83 |
0.006595 |
0.2588 |
bsu17210 |
pksN |
-11.93 |
0.108023 |
61 -- 115 |
29 -- 70 |
polyketide synthase PksN |
|
_____________60_______________________________________________________116\n_____________|________________________________________________________|\n5'-AGC...CAAGA____________GAAA__________AA__G_G__CAAAACAUC____A____A__AAAUC...UAU-3'\n______________GGUGGCGCUAGG_____UUGGAUUUU__GA_U_UG_________AUAG_AGGG_GU\n______________|||:||||:|:|_____|:||||:||__||_|_||_________||||_||||_||\n______________CCAUCGCGGUUC_____AGCCUAGAA__CU_A_AC_________UAUC_UCCC_CA\n3'-UUU...CGCCC____________AAAAA_______________G_______________________ACCCC...AUA-5'\n_____________|________________________________________________________|\n_____________71_______________________________________________________28\n |
79 -- 85 |
45 -- 51 |
-37.7722 kcal/mol |
10.06 kcal/mol |
15.7778 kcal/mol |
60 116@LB@ | |@LB@5'-AGC...CAAGA GAAA AA G G CAAAACAUC A A AAAUC...UAU-3'@LB@ GGUGGCGCUAGG UUGGAUUUU GA U UG AUAG AGGG GU@LB@ |||:||||:|:| |:||||:|| || | || |||| |||| ||@LB@ CCAUCGCGGUUC AGCCUAGAA CU A AC UAUC UCCC CA@LB@3'-UUU...CGCCC AAAAA G ACCCC...AUA-5'@LB@ | |@LB@ 71 28@LB@@LB@@LB@Energy -11.93 kcal/mol@LB@Hybridization Energy -37.7722 kcal/mol@LB@Unfolding Energy - mRNA 10.06 kcal/mol@LB@Unfolding Energy - sRNA 15.7778 kcal/mol@LB@@LB@Position - mRNA 61 -- 115@LB@Position - sRNA 29 -- 70@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 45 -- 51@LB@ |
