Input and runtime details for job 5774122 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5774122 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:30:38 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:16:08 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 23:32:42 CET 2018
	Post-Processing Finished		@ Fri Feb 16 23:32:50 CET 2018
	Job Completed		@ Fri Feb 16 23:33:10 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5774122&toolName=CopraRNA ( 30 days expiry )

Description of the job

FsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000964 Bacillus subtilis subsp. subtilis str. 168

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
141	0.01225	0.2883	bsu32310	yutD	-11.41	0.131333	145 -- 158	24 -- 40	Evidence 4: Homologs of previously reported genes of unknown function; PubMedId: 12770717	_____________144_______________159\n_____________\|_________________\|\n5'-ACA...GCAAU________AC_______CAAUG...AUA-3'\n______________UGAUGGGG_____GGGG\n______________\|\|\|\|:\|:\|_____\|\|\|\|\n______________ACUAUCUC_____CCCC\n3'-UUU...ACUAG________CCCAA____UUGUC...AUA-5'\n_____________\|_________________\|\n_____________41________________23\n	146 -- 152	33 -- 39	-14.827 kcal/mol	2.62921 kcal/mol	0.790979 kcal/mol	144 159@LB@ \| \|@LB@5'-ACA...GCAAU AC CAAUG...AUA-3'@LB@ UGAUGGGG GGGG@LB@ \|\|\|\|:\|:\| \|\|\|\|@LB@ ACUAUCUC CCCC@LB@3'-UUU...ACUAG CCCAA UUGUC...AUA-5'@LB@ \| \|@LB@ 41 23@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -14.827 kcal/mol@LB@Unfolding Energy - mRNA 2.62921 kcal/mol@LB@Unfolding Energy - sRNA 0.790979 kcal/mol@LB@@LB@Position - mRNA 145 -- 158@LB@Position - sRNA 24 -- 40@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 33 -- 39@LB@
98	0.008247	0.2778	bsu19360	odhB	-11.30	0.136272	146 -- 191	23 -- 51	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex	_____________145____________________________________________192\n_____________\|______________________________________________\|\n5'-AAG...GAACA___ACGUA___U_________CUU_ACUCGCAAAAACUAAG_____AAUGA...AAU-3'\n______________GGA_____UUG_AUCUGAUAG___G________________GGGGA\n______________\|\|\|_____\|\|\|_\|\|\|\|\|\|\|\|\|___\|________________\|\|\|\|\|\n______________CCU_____AAC_UAGACUAUC___C________________CCCCU\n3'-UUU...AAAAG___AG________________UCC_CAA__________________UGUCU...AUA-5'\n_____________\|______________________________________________\|\n_____________52_____________________________________________22\n	160 -- 166	35 -- 41	-19.511 kcal/mol	7.22607 kcal/mol	0.980049 kcal/mol	145 192@LB@ \| \|@LB@5'-AAG...GAACA ACGUA U CUU ACUCGCAAAAACUAAG AAUGA...AAU-3'@LB@ GGA UUG AUCUGAUAG G GGGGA@LB@ \|\|\| \|\|\| \|\|\|\|\|\|\|\|\| \| \|\|\|\|\|@LB@ CCU AAC UAGACUAUC C CCCCU@LB@3'-UUU...AAAAG AG UCC CAA UGUCU...AUA-5'@LB@ \| \|@LB@ 52 22@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -19.511 kcal/mol@LB@Unfolding Energy - mRNA 7.22607 kcal/mol@LB@Unfolding Energy - sRNA 0.980049 kcal/mol@LB@@LB@Position - mRNA 146 -- 191@LB@Position - sRNA 23 -- 51@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 35 -- 41@LB@
149	0.01325	0.2966	bsu07610	citM	-7.43	0.445360	149 -- 162	15 -- 27	Mg(2+)/citrate complex secondary transporter	_____________148____________163\n_____________\|______________\|\n5'-AUU...AAAAA_______A______AAAAA...UUG-3'\n______________GGGGAGU_AGGGAG\n______________\|\|\|\|\|::_\|\|:\|\|\|\n______________CCCCUUG_UCUCUC\n3'-UUU...CCCAA______________AUCGA...AUA-5'\n_____________\|______________\|\n_____________28_____________14\n	149 -- 155	21 -- 27	-17.4184 kcal/mol	3.9771 kcal/mol	6.01129 kcal/mol	148 163@LB@ \| \|@LB@5'-AUU...AAAAA A AAAAA...UUG-3'@LB@ GGGGAGU AGGGAG@LB@ \|\|\|\|\|:: \|\|:\|\|\|@LB@ CCCCUUG UCUCUC@LB@3'-UUU...CCCAA AUCGA...AUA-5'@LB@ \| \|@LB@ 28 14@LB@@LB@@LB@Energy -7.43 kcal/mol@LB@Hybridization Energy -17.4184 kcal/mol@LB@Unfolding Energy - mRNA 3.9771 kcal/mol@LB@Unfolding Energy - sRNA 6.01129 kcal/mol@LB@@LB@Position - mRNA 149 -- 162@LB@Position - sRNA 15 -- 27@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 21 -- 27@LB@
194	0.01929	0.3269	bsu14860	pycA	-10.87	0.159030	149 -- 193	23 -- 51	pyruvate carboxylase	_____________148___________________________________________194\n_____________\|_____________________________________________\|\n5'-GGG...GGGAA____AUUCCGCUA_______UUAUAUUCCAUUC____AAG_____GAAAA...UUG-3'\n______________GGAU_________UGAUAGA_____________GGGU___GGGGA\n______________\|\|\|\|_________\|\|\|\|\|\|\|_____________\|\|\|\|___\|\|\|\|\|\n______________CCUA_________ACUAUCU_____________CCCA___CCCCU\n3'-UUU...AAAAG____GAACUAG_________C________________A_______UGUCU...AUA-5'\n_____________\|_____________________________________________\|\n_____________52____________________________________________22\n	162 -- 168	34 -- 40	-22.9068 kcal/mol	11.0566 kcal/mol	0.980049 kcal/mol	148 194@LB@ \| \|@LB@5'-GGG...GGGAA AUUCCGCUA UUAUAUUCCAUUC AAG GAAAA...UUG-3'@LB@ GGAU UGAUAGA GGGU GGGGA@LB@ \|\|\|\| \|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|@LB@ CCUA ACUAUCU CCCA CCCCU@LB@3'-UUU...AAAAG GAACUAG C A UGUCU...AUA-5'@LB@ \| \|@LB@ 52 22@LB@@LB@@LB@Energy -10.87 kcal/mol@LB@Hybridization Energy -22.9068 kcal/mol@LB@Unfolding Energy - mRNA 11.0566 kcal/mol@LB@Unfolding Energy - sRNA 0.980049 kcal/mol@LB@@LB@Position - mRNA 149 -- 193@LB@Position - sRNA 23 -- 51@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 34 -- 40@LB@
29	0.0009972	0.1113	bsu38140	qoxD	-9.49	0.251109	150 -- 190	24 -- 51	quinol oxidase subunit 4	_____________149_______________________________________191\n_____________\|_________________________________________\|\n5'-UUU...UGUAU_______C____UUAC___UGUCUAUCUCAU___A______GGUCU...CAG-3'\n______________GGAUUUU_AUCU____AGG____________GGG_UUGGGG\n______________\|\|\|\|:\|\|_\|\|\|\|____\|\|:____________\|\|\|_\|\|\|\|\|\|\n______________CCUAGAA_UAGA____UCU____________CCC_AACCCC\n3'-UUU...AAAAG_______C____CUA___________________C______UUGUC...AUA-5'\n_____________\|_________________________________________\|\n_____________52________________________________________23\n	150 -- 156	45 -- 51	-23.5355 kcal/mol	13.0692 kcal/mol	0.978921 kcal/mol	149 191@LB@ \| \|@LB@5'-UUU...UGUAU C UUAC UGUCUAUCUCAU A GGUCU...CAG-3'@LB@ GGAUUUU AUCU AGG GGG UUGGGG@LB@ \|\|\|\|:\|\| \|\|\|\| \|\|: \|\|\| \|\|\|\|\|\|@LB@ CCUAGAA UAGA UCU CCC AACCCC@LB@3'-UUU...AAAAG C CUA C UUGUC...AUA-5'@LB@ \| \|@LB@ 52 23@LB@@LB@@LB@Energy -9.49 kcal/mol@LB@Hybridization Energy -23.5355 kcal/mol@LB@Unfolding Energy - mRNA 13.0692 kcal/mol@LB@Unfolding Energy - sRNA 0.978921 kcal/mol@LB@@LB@Position - mRNA 150 -- 190@LB@Position - sRNA 24 -- 51@LB@Position Seed - mRNA 150 -- 156@LB@Position Seed - sRNA 45 -- 51@LB@
148	0.01313	0.296	bsu02100	cypC	-16.43	0.015277	151 -- 198	11 -- 53	fatty-acid peroxygenase	_____________150______________________________________________199\n_____________\|________________________________________________\|\n5'-CAA...UAUAG_____AGAUAACG___AAA_______UAAAAA____G_C_____A___GGAUG...AUU-3'\n______________UCGGA________UGA___UAGAGGG______GGAA_G_AGGGU_AGC\n______________\|\|\|\|\|________\|\|\|___\|\|\|\|\|\|\|______\|\|\|\|_:_\|\|:\|\|_\|\|\|\n______________AGCCU________ACU___AUCUCCC______CCUU_U_UCUCA_UCG\n3'-UUU...AAAAA_____AGAACUAG_____________CAACC_____G_C_________AAGAG...AUA-5'\n_____________\|________________________________________________\|\n_____________54_______________________________________________10\n	170 -- 176	31 -- 37	-31.9294 kcal/mol	5.5363 kcal/mol	9.96369 kcal/mol	150 199@LB@ \| \|@LB@5'-CAA...UAUAG AGAUAACG AAA UAAAAA G C A GGAUG...AUU-3'@LB@ UCGGA UGA UAGAGGG GGAA G AGGGU AGC@LB@ \|\|\|\|\| \|\|\| \|\|\|\|\|\|\| \|\|\|\| : \|\|:\|\| \|\|\|@LB@ AGCCU ACU AUCUCCC CCUU U UCUCA UCG@LB@3'-UUU...AAAAA AGAACUAG CAACC G C AAGAG...AUA-5'@LB@ \| \|@LB@ 54 10@LB@@LB@@LB@Energy -16.43 kcal/mol@LB@Hybridization Energy -31.9294 kcal/mol@LB@Unfolding Energy - mRNA 5.5363 kcal/mol@LB@Unfolding Energy - sRNA 9.96369 kcal/mol@LB@@LB@Position - mRNA 151 -- 198@LB@Position - sRNA 11 -- 53@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 31 -- 37@LB@
8	5.206e-05	0.02171	bsu37180	fadF	-12.43	0.089383	154 -- 199	26 -- 74	'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 17189250; Product type pe : enzyme'	_____________153____________________________________________________200\n_____________\|______________________________________________________\|\n5'-CAA...AGAUU_________G_GA___________C______U___A_UAAAA______AA____AAUGA...ACA-3'\n______________CGGGGGUAG_G__________UCG_GGUCUU_AUC_G_____AGGGGG__UUGG\n______________\|\|\|\|\|\|\|\|\|_\|__________\|\|\|_\|:\|\|\|\|_\|\|\|_\|_____\|\|:\|\|\|__\|\|\|\|\n______________GCCCCCAUC_C__________AGC_CUAGAA_UAG_C_____UCUCCC__AACC\n3'-UUU...GAACC_________G_GGUUCAAAAA__________C___A_UA_________C_____CCUUG...AUA-5'\n_____________\|______________________________________________________\|\n_____________75_____________________________________________________25\n	156 -- 162	66 -- 72	-40.4869 kcal/mol	12.2576 kcal/mol	15.8011 kcal/mol	153 200@LB@ \| \|@LB@5'-CAA...AGAUU G GA C U A UAAAA AA AAUGA...ACA-3'@LB@ CGGGGGUAG G UCG GGUCUU AUC G AGGGGG UUGG@LB@ \|\|\|\|\|\|\|\|\| \| \|\|\| \|:\|\|\|\| \|\|\| \| \|\|:\|\|\| \|\|\|\|@LB@ GCCCCCAUC C AGC CUAGAA UAG C UCUCCC AACC@LB@3'-UUU...GAACC G GGUUCAAAAA C A UA C CCUUG...AUA-5'@LB@ \| \|@LB@ 75 25@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -40.4869 kcal/mol@LB@Unfolding Energy - mRNA 12.2576 kcal/mol@LB@Unfolding Energy - sRNA 15.8011 kcal/mol@LB@@LB@Position - mRNA 154 -- 199@LB@Position - sRNA 26 -- 74@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 66 -- 72@LB@
57	0.003091	0.1809	bsu19720	yodR	-13.42	0.059995	155 -- 217	11 -- 51	coenzyme A transferase subunit beta	_____________154_________________________________________________________________218\n_____________\|___________________________________________________________________\|\n5'-GCU...ACCGG_______UG________________UACGAAGCGA____GUGAAGUGGAAAUGG__UU_________AGAAA...CCU-3'\n______________GGGUUUU___UUGA___GGGGGUUG__________GGGA_______________GC__GGGAGUAGC\n______________\|\|:\|:\|\|___:\|\|\|___:\|\|\|\|\|\|\|__________\|\|\|\|_______________:\|__\|:\|\|\|\|\|\|\|\n______________CCUAGAA___GACU___UCCCCAAC__________CCCU_______________UG__CUCUCAUCG\n3'-UUU...AAAAG_______CUA____AUC_______________________________________U__________AAGAG...AUA-5'\n_____________\|___________________________________________________________________\|\n_____________52__________________________________________________________________10\n	211 -- 217	11 -- 17	-37.5623 kcal/mol	14.1868 kcal/mol	9.95641 kcal/mol	154 218@LB@ \| \|@LB@5'-GCU...ACCGG UG UACGAAGCGA GUGAAGUGGAAAUGG UU AGAAA...CCU-3'@LB@ GGGUUUU UUGA GGGGGUUG GGGA GC GGGAGUAGC@LB@ \|\|:\|:\|\| :\|\|\| :\|\|\|\|\|\|\| \|\|\|\| :\| \|:\|\|\|\|\|\|\|@LB@ CCUAGAA GACU UCCCCAAC CCCU UG CUCUCAUCG@LB@3'-UUU...AAAAG CUA AUC U AAGAG...AUA-5'@LB@ \| \|@LB@ 52 10@LB@@LB@@LB@Energy -13.42 kcal/mol@LB@Hybridization Energy -37.5623 kcal/mol@LB@Unfolding Energy - mRNA 14.1868 kcal/mol@LB@Unfolding Energy - sRNA 9.95641 kcal/mol@LB@@LB@Position - mRNA 155 -- 217@LB@Position - sRNA 11 -- 51@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 11 -- 17@LB@
63	0.003897	0.2064	bsu14900	ctaD	-14.39	0.039562	155 -- 192	22 -- 52	cytochrome c oxidase subunit 1	_____________154____________________________________193\n_____________\|______________________________________\|\n5'-GUG...AAAAG____AAGCAA__AG__A___AA___AGAUA________GGUCA...UGG-3'\n______________CGGA______GU__GG_AGA__GGG_____UUGGGGGA\n______________\|\|\|\|______:\|__\|:_\|\|\|__\|\|\|_____\|\|\|\|\|\|:\|\n______________GCCU______UA__CU_UCU__CCC_____AACCCCUU\n3'-UUU...AAAAA____AGAAC___GA__A___C_________________GUCUC...AUA-5'\n_____________\|______________________________________\|\n_____________53_____________________________________21\n	186 -- 192	22 -- 28	-20.3858 kcal/mol	4.11152 kcal/mol	1.8808 kcal/mol	154 193@LB@ \| \|@LB@5'-GUG...AAAAG AAGCAA AG A AA AGAUA GGUCA...UGG-3'@LB@ CGGA GU GG AGA GGG UUGGGGGA@LB@ \|\|\|\| :\| \|: \|\|\| \|\|\| \|\|\|\|\|\|:\|@LB@ GCCU UA CU UCU CCC AACCCCUU@LB@3'-UUU...AAAAA AGAAC GA A C GUCUC...AUA-5'@LB@ \| \|@LB@ 53 21@LB@@LB@@LB@Energy -14.39 kcal/mol@LB@Hybridization Energy -20.3858 kcal/mol@LB@Unfolding Energy - mRNA 4.11152 kcal/mol@LB@Unfolding Energy - sRNA 1.8808 kcal/mol@LB@@LB@Position - mRNA 155 -- 192@LB@Position - sRNA 22 -- 52@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 22 -- 28@LB@
111	0.009313	0.2778	bsu16780	rnjB	-13.26	0.064171	155 -- 177	29 -- 51	ribonuclease J 2	_____________154_____________________178\n_____________\|_______________________\|\n5'-GCA...UAAUA___ACAAGUGA_______C____AUGAG...UAU-3'\n______________GGA________UGAUGGA_GGGU\n______________\|\|\|________\|\|\|\|:\|\|_\|\|\|\|\n______________CCU________ACUAUCU_CCCA\n3'-UUU...AAAAG___AGAACUAG_______C____ACCCC...AUA-5'\n_____________\|_______________________\|\n_____________52______________________28\n	166 -- 172	34 -- 40	-18.6769 kcal/mol	4.44629 kcal/mol	0.974539 kcal/mol	154 178@LB@ \| \|@LB@5'-GCA...UAAUA ACAAGUGA C AUGAG...UAU-3'@LB@ GGA UGAUGGA GGGU@LB@ \|\|\| \|\|\|\|:\|\| \|\|\|\|@LB@ CCU ACUAUCU CCCA@LB@3'-UUU...AAAAG AGAACUAG C ACCCC...AUA-5'@LB@ \| \|@LB@ 52 28@LB@@LB@@LB@Energy -13.26 kcal/mol@LB@Hybridization Energy -18.6769 kcal/mol@LB@Unfolding Energy - mRNA 4.44629 kcal/mol@LB@Unfolding Energy - sRNA 0.974539 kcal/mol@LB@@LB@Position - mRNA 155 -- 177@LB@Position - sRNA 29 -- 51@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 34 -- 40@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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