Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
137	0.006282	0.1605	b0123	cueO	-13.57	0.047118	144 -- 176	48 -- 78	multicopper oxidase (laccase)	_____________143________________________________177\n_____________\|__________________________________\|\n5'-CUG...AGGGG___________U__A_______AUU_________CUGCU...CGU-3'\n______________AAGGACUAUGC_CA_C_GUUUG___UUGUUUCGC\n______________\|\|:\|\|\|\|\|::\|_\|\|_\|_:\|\|:\|___\|\|\|\|\|\|\|:\|\n______________UUUCUGAUGUG_GU_G_UAAGC___AACAAAGUG\n3'-UUU...ACCCG___________U__C_U_________________AGUCC...GCA-5'\n_____________\|__________________________________\|\n_____________79_________________________________47\n	170 -- 176	48 -- 54	-24.1368 kcal/mol	6.88378 kcal/mol	3.68736 kcal/mol	143 177@LB@ \| \|@LB@5'-CUG...AGGGG U A AUU CUGCU...CGU-3'@LB@ AAGGACUAUGC CA C GUUUG UUGUUUCGC@LB@ \|\|:\|\|\|\|\|::\| \|\| \| :\|\|:\| \|\|\|\|\|\|\|:\|@LB@ UUUCUGAUGUG GU G UAAGC AACAAAGUG@LB@3'-UUU...ACCCG U C U AGUCC...GCA-5'@LB@ \| \|@LB@ 79 47@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -24.1368 kcal/mol@LB@Unfolding Energy - mRNA 6.88378 kcal/mol@LB@Unfolding Energy - sRNA 3.68736 kcal/mol@LB@@LB@Position - mRNA 144 -- 176@LB@Position - sRNA 48 -- 78@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 48 -- 54@LB@
23	0.0001955	0.02926	b3846	fadB	-13.56	0.047358	39 -- 83	35 -- 77	fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase	_____________38________________________________________________84\n_____________\|_________________________________________________\|\n5'-AGU...ACCUU___U__C_____CUU____CA____________C___A_C_U_______GCCGA...CUG-3'\n______________AAG_CU_CGCAU___AGCA__UCGU____UCAU_CAG_G_G_GAUUUCU\n______________\|\|\|_\|\|_\|:\|\|:___\|\|\|\|__\|\|\|\|____\|\|\|:_\|\|\|_\|_\|_\|\|\|\|\|\|:\n______________UUC_GA_GUGUG___UCGU__AGCA____AGUG_GUC_C_C_CUAAAGG\n3'-UUU...CCCGU___U__U____________UA____ACAA____A_______________UACGA...GCA-5'\n_____________\|_________________________________________________\|\n_____________78________________________________________________34\n	77 -- 83	35 -- 41	-27.7125 kcal/mol	9.35112 kcal/mol	4.80542 kcal/mol	38 84@LB@ \| \|@LB@5'-AGU...ACCUU U C CUU CA C A C U GCCGA...CUG-3'@LB@ AAG CU CGCAU AGCA UCGU UCAU CAG G G GAUUUCU@LB@ \|\|\| \|\| \|:\|\|: \|\|\|\| \|\|\|\| \|\|\|: \|\|\| \| \| \|\|\|\|\|\|:@LB@ UUC GA GUGUG UCGU AGCA AGUG GUC C C CUAAAGG@LB@3'-UUU...CCCGU U U UA ACAA A UACGA...GCA-5'@LB@ \| \|@LB@ 78 34@LB@@LB@@LB@Energy -13.56 kcal/mol@LB@Hybridization Energy -27.7125 kcal/mol@LB@Unfolding Energy - mRNA 9.35112 kcal/mol@LB@Unfolding Energy - sRNA 4.80542 kcal/mol@LB@@LB@Position - mRNA 39 -- 83@LB@Position - sRNA 35 -- 77@LB@Position Seed - mRNA 77 -- 83@LB@Position Seed - sRNA 35 -- 41@LB@
123	0.005464	0.1553	b3746	ravA	-13.51	0.048673	243 -- 263	41 -- 65	hexameric AAA+ MoxR family ATPase putative molecular chaperone	_____________242_______________________264\n_____________\|_________________________\|\n5'-UGA...GCCUG____________GAAA_________CUUUA...UAU-3'\n______________AGCAGUUCGUUG________AGGGG\n______________\|\|\|\|:\|\|\|\|\|\|\|________\|\|\|\|\|\n______________UCGUUAAGCAAC________UCCCC\n3'-UUU...GUGUG____________AAAGUGAG_____UAAAG...GCA-5'\n_____________\|_________________________\|\n_____________66________________________40\n	248 -- 254	54 -- 60	-25.3509 kcal/mol	7.14336 kcal/mol	4.70281 kcal/mol	242 264@LB@ \| \|@LB@5'-UGA...GCCUG GAAA CUUUA...UAU-3'@LB@ AGCAGUUCGUUG AGGGG@LB@ \|\|\|\|:\|\|\|\|\|\|\| \|\|\|\|\|@LB@ UCGUUAAGCAAC UCCCC@LB@3'-UUU...GUGUG AAAGUGAG UAAAG...GCA-5'@LB@ \| \|@LB@ 66 40@LB@@LB@@LB@Energy -13.51 kcal/mol@LB@Hybridization Energy -25.3509 kcal/mol@LB@Unfolding Energy - mRNA 7.14336 kcal/mol@LB@Unfolding Energy - sRNA 4.70281 kcal/mol@LB@@LB@Position - mRNA 243 -- 263@LB@Position - sRNA 41 -- 65@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 54 -- 60@LB@
73	0.00298	0.1437	b0474	adk	-13.48	0.049381	180 -- 198	34 -- 50	adenylate kinase	_____________179_________________199\n_____________\|___________________\|\n5'-ACC...CGACA_____UA____________CAAUG...AUA-3'\n______________CAUUU__AGGGGAUUUUCG\n______________\|\|:\|:__\|\|\|\|\|\|\|\|\|:\|:\n______________GUGAG__UCCCCUAAAGGU\n3'-UUU...ACAAA___________________ACGAA...GCA-5'\n_____________\|___________________\|\n_____________51__________________33\n	191 -- 197	35 -- 41	-19.9961 kcal/mol	4.64453 kcal/mol	1.87382 kcal/mol	179 199@LB@ \| \|@LB@5'-ACC...CGACA UA CAAUG...AUA-3'@LB@ CAUUU AGGGGAUUUUCG@LB@ \|\|:\|: \|\|\|\|\|\|\|\|\|:\|:@LB@ GUGAG UCCCCUAAAGGU@LB@3'-UUU...ACAAA ACGAA...GCA-5'@LB@ \| \|@LB@ 51 33@LB@@LB@@LB@Energy -13.48 kcal/mol@LB@Hybridization Energy -19.9961 kcal/mol@LB@Unfolding Energy - mRNA 4.64453 kcal/mol@LB@Unfolding Energy - sRNA 1.87382 kcal/mol@LB@@LB@Position - mRNA 180 -- 198@LB@Position - sRNA 34 -- 50@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 35 -- 41@LB@
61	0.002486	0.1417	b1487	ddpA	-13.46	0.049908	168 -- 192	43 -- 71	D D-dipeptide ABC transporter periplasmic binding protein	_____________167___________________________193\n_____________\|_____________________________\|\n5'-UCA...CCCUG________________CAG____A_____AAACU...UGA-3'\n______________GCACACAGCA___CGU____UUA_UCAGG\n______________:\|\|\|\|\|\|\|\|\|___\|\|\|____\|:\|_\|\|\|\|\|\n______________UGUGUGUCGU___GCA____AGU_AGUCC\n3'-UUU...UCUGA__________UAA___ACAA___G_____CCUAA...GCA-5'\n_____________\|_____________________________\|\n_____________72____________________________42\n	171 -- 177	62 -- 68	-25.043 kcal/mol	7.26137 kcal/mol	4.32409 kcal/mol	167 193@LB@ \| \|@LB@5'-UCA...CCCUG CAG A AAACU...UGA-3'@LB@ GCACACAGCA CGU UUA UCAGG@LB@ :\|\|\|\|\|\|\|\|\| \|\|\| \|:\| \|\|\|\|\|@LB@ UGUGUGUCGU GCA AGU AGUCC@LB@3'-UUU...UCUGA UAA ACAA G CCUAA...GCA-5'@LB@ \| \|@LB@ 72 42@LB@@LB@@LB@Energy -13.46 kcal/mol@LB@Hybridization Energy -25.043 kcal/mol@LB@Unfolding Energy - mRNA 7.26137 kcal/mol@LB@Unfolding Energy - sRNA 4.32409 kcal/mol@LB@@LB@Position - mRNA 168 -- 192@LB@Position - sRNA 43 -- 71@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 62 -- 68@LB@
91	0.00412	0.1538	b0931	pncB	-13.41	0.051237	255 -- 284	48 -- 77	nicotinate phosphoribosyltransferase	_____________254______________________________285\n_____________\|________________________________\|\n5'-AUG...CUUAU__________AU_______GC___________CACUA...UGC-3'\n______________AAG_UUGCAU__GCAGCAA__CG_UGUUUCAU\n______________\|\|\|_:\|:\|\|:__:\|\|\|\|\|\|__\|\|_\|\|\|\|\|\|\|:\n______________UUC_GAUGUG__UGUCGUU__GC_ACAAAGUG\n3'-UUU...CCCGU___U_______________AA__A________AGUCC...GCA-5'\n_____________\|________________________________\|\n_____________78_______________________________47\n	278 -- 284	48 -- 54	-26.8209 kcal/mol	9.72536 kcal/mol	3.68729 kcal/mol	254 285@LB@ \| \|@LB@5'-AUG...CUUAU AU GC CACUA...UGC-3'@LB@ AAG UUGCAU GCAGCAA CG UGUUUCAU@LB@ \|\|\| :\|:\|\|: :\|\|\|\|\|\| \|\| \|\|\|\|\|\|\|:@LB@ UUC GAUGUG UGUCGUU GC ACAAAGUG@LB@3'-UUU...CCCGU U AA A AGUCC...GCA-5'@LB@ \| \|@LB@ 78 47@LB@@LB@@LB@Energy -13.41 kcal/mol@LB@Hybridization Energy -26.8209 kcal/mol@LB@Unfolding Energy - mRNA 9.72536 kcal/mol@LB@Unfolding Energy - sRNA 3.68729 kcal/mol@LB@@LB@Position - mRNA 255 -- 284@LB@Position - sRNA 48 -- 77@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 48 -- 54@LB@
51	0.001671	0.1153	b3527	yhjJ	-13.34	0.053290	153 -- 204	31 -- 75	putative periplasmic M16 family chaperone	_____________152_____________________________________________________205\n_____________\|_______________________________________________________\|\n5'-CUC...UGACC___A___G_____UU___UAUUACCAGGAUA___GA____________CA_____AGGGC...GGC-3'\n______________GAC_ACG_GCAGU__UUU_____________GUU____UCAGGGG_UU__CAUGC\n______________\|\|\|_\|\|:_:\|\|\|:__\|\|:_____________\|\|\|____\|\|\|\|\|\|\|_\|\|__\|\|\|\|\|\n______________CUG_UGU_UGUCG__AAG_____________CAA____AGUCCCC_AA__GUACG\n3'-UUU...CGUUU___A___G_____UU___CAA_____________AGUG_______U__AG_____AAUAU...GCA-5'\n_____________\|_______________________________________________________\|\n_____________76______________________________________________________30\n	189 -- 195	41 -- 47	-32.6504 kcal/mol	12.0549 kcal/mol	7.26183 kcal/mol	152 205@LB@ \| \|@LB@5'-CUC...UGACC A G UU UAUUACCAGGAUA GA CA AGGGC...GGC-3'@LB@ GAC ACG GCAGU UUU GUU UCAGGGG UU CAUGC@LB@ \|\|\| \|\|: :\|\|\|: \|\|: \|\|\| \|\|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ CUG UGU UGUCG AAG CAA AGUCCCC AA GUACG@LB@3'-UUU...CGUUU A G UU CAA AGUG U AG AAUAU...GCA-5'@LB@ \| \|@LB@ 76 30@LB@@LB@@LB@Energy -13.34 kcal/mol@LB@Hybridization Energy -32.6504 kcal/mol@LB@Unfolding Energy - mRNA 12.0549 kcal/mol@LB@Unfolding Energy - sRNA 7.26183 kcal/mol@LB@@LB@Position - mRNA 153 -- 204@LB@Position - sRNA 31 -- 75@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 41 -- 47@LB@
46	0.001357	0.1038	b1235	rssB	-13.33	0.053430	184 -- 194	40 -- 50	PcnB-degradosome interaction factor; response regulator	_____________183_________195\n_____________\|___________\|\n5'-AAU...AAAGC__G________GAGAA...CAA-3'\n______________CA_UCAGGGGA\n______________\|\|_\|\|\|\|\|\|\|\|\n______________GU_AGUCCCCU\n3'-UUU...ACAAA__G________AAAGG...GCA-5'\n_____________\|___________\|\n_____________51__________39\n	188 -- 194	40 -- 46	-19.1981 kcal/mol	4.11697 kcal/mol	1.75243 kcal/mol	183 195@LB@ \| \|@LB@5'-AAU...AAAGC G GAGAA...CAA-3'@LB@ CA UCAGGGGA@LB@ \|\| \|\|\|\|\|\|\|\|@LB@ GU AGUCCCCU@LB@3'-UUU...ACAAA G AAAGG...GCA-5'@LB@ \| \|@LB@ 51 39@LB@@LB@@LB@Energy -13.33 kcal/mol@LB@Hybridization Energy -19.1981 kcal/mol@LB@Unfolding Energy - mRNA 4.11697 kcal/mol@LB@Unfolding Energy - sRNA 1.75243 kcal/mol@LB@@LB@Position - mRNA 184 -- 194@LB@Position - sRNA 40 -- 50@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 40 -- 46@LB@
184	0.00937	0.1776	b2467	nudK	-13.30	0.054361	69 -- 94	38 -- 78	GDP-mannose pyrophosphatase	_____________68________________________________________95\n_____________\|_________________________________________\|\n5'-CAA...GGCAG______GU_________________________________AUUGA...AAG-3'\n______________AAGGAC__CAUGCGGCA_______________CAGGGGAUU\n______________\|\|:\|\|\|__\|\|::\|:\|\|\|_______________\|\|\|\|\|\|\|\|\|\n______________UUUCUG__GUGUGUCGU_______________GUCCCCUAA\n3'-UUU...ACCCG______AU_________UAAGCAACAAAGUGA_________AGGUA...GCA-5'\n_____________\|_________________________________________\|\n_____________79________________________________________37\n	88 -- 94	38 -- 44	-30.5001 kcal/mol	12.4248 kcal/mol	4.77973 kcal/mol	68 95@LB@ \| \|@LB@5'-CAA...GGCAG GU AUUGA...AAG-3'@LB@ AAGGAC CAUGCGGCA CAGGGGAUU@LB@ \|\|:\|\|\| \|\|::\|:\|\|\| \|\|\|\|\|\|\|\|\|@LB@ UUUCUG GUGUGUCGU GUCCCCUAA@LB@3'-UUU...ACCCG AU UAAGCAACAAAGUGA AGGUA...GCA-5'@LB@ \| \|@LB@ 79 37@LB@@LB@@LB@Energy -13.30 kcal/mol@LB@Hybridization Energy -30.5001 kcal/mol@LB@Unfolding Energy - mRNA 12.4248 kcal/mol@LB@Unfolding Energy - sRNA 4.77973 kcal/mol@LB@@LB@Position - mRNA 69 -- 94@LB@Position - sRNA 38 -- 78@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 38 -- 44@LB@
41	0.0008963	0.07693	b1378	pfo	-13.27	0.055135	136 -- 164	58 -- 78	pyruvate-flavodoxin oxidoreductase	_____________135___________________________165\n_____________\|_____________________________\|\n5'-GCU...AAUUG_________UACUGGGC____________CGUGC...CAG-3'\n______________AAAGAUUAC________GCGCAGCAAUUU\n______________\|\|\|\|\|:\|\|\|________:\|:\|\|\|\|\|\|\|\|:\n______________UUUCUGAUG________UGUGUCGUUAAG\n3'-UUU...ACCCG_____________________________CAACA...GCA-5'\n_____________\|_____________________________\|\n_____________79____________________________57\n	158 -- 164	58 -- 64	-22.0871 kcal/mol	5.50788 kcal/mol	3.31018 kcal/mol	135 165@LB@ \| \|@LB@5'-GCU...AAUUG UACUGGGC CGUGC...CAG-3'@LB@ AAAGAUUAC GCGCAGCAAUUU@LB@ \|\|\|\|\|:\|\|\| :\|:\|\|\|\|\|\|\|\|:@LB@ UUUCUGAUG UGUGUCGUUAAG@LB@3'-UUU...ACCCG CAACA...GCA-5'@LB@ \| \|@LB@ 79 57@LB@@LB@@LB@Energy -13.27 kcal/mol@LB@Hybridization Energy -22.0871 kcal/mol@LB@Unfolding Energy - mRNA 5.50788 kcal/mol@LB@Unfolding Energy - sRNA 3.31018 kcal/mol@LB@@LB@Position - mRNA 136 -- 164@LB@Position - sRNA 58 -- 78@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 58 -- 64@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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