Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
182	0.009082	0.1747	b3998	nfi	-11.38	0.137700	53 -- 76	45 -- 75	endonuclease V; deoxyinosine 3' endonuclease		_____________52______________________________77\n_____________\|_______________________________\|\n5'-UGG...GUAGC________UU_________________G___CGGCG...GAG-3'\n______________GACUAUAC___GCAG______GUUUCG_UCA\n______________\|\|\|\|\|:\|\|___\|\|\|:______\|\|\|\|\|:_\|\|\|\n______________CUGAUGUG___CGUU______CAAAGU_AGU\n3'-UUU...CGUUU________UGU____AAGCAA______G___CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	54 -- 60	68 -- 74	-21.6188 kcal/mol	6.49773 kcal/mol	3.74078 kcal/mol	52 77@LB@ \| \|@LB@5'-UGG...GUAGC UU G CGGCG...GAG-3'@LB@ GACUAUAC GCAG GUUUCG UCA@LB@ \|\|\|\|\|:\|\| \|\|\|: \|\|\|\|\|: \|\|\|@LB@ CUGAUGUG CGUU CAAAGU AGU@LB@3'-UUU...CGUUU UGU AAGCAA G CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -11.38 kcal/mol@LB@Hybridization Energy -21.6188 kcal/mol@LB@Unfolding Energy - mRNA 6.49773 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 53 -- 76@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 68 -- 74@LB@
59	0.002304	0.1363	b2508	guaB	-11.36	0.139274	1 -- 26	38 -- 71	IMP dehydrogenase		______________1__________________________________27\n______________\|__________________________________\|\n___________5'-__UA_______AA____________G_________GAGAA...AGC-3'\n______________AC__ACGGUAA___________AUU_CAGGGGAUU\n______________\|\|__\|\|:\|:\|\|___________\|:\|_\|\|\|\|\|\|\|\|\|\n______________UG__UGUCGUU___________UGA_GUCCCCUAA\n3'-UUU...UCUGA__UG_______AAGCAACAAAG_____________AGGUA...GCA-5'\n_____________\|___________________________________\|\n_____________72__________________________________37\n	20 -- 26	38 -- 44	-21.1303 kcal/mol	5.02575 kcal/mol	4.74986 kcal/mol	1 27@LB@ \| \|@LB@ 5'- UA AA G GAGAA...AGC-3'@LB@ AC ACGGUAA AUU CAGGGGAUU@LB@ \|\| \|\|:\|:\|\| \|:\| \|\|\|\|\|\|\|\|\|@LB@ UG UGUCGUU UGA GUCCCCUAA@LB@3'-UUU...UCUGA UG AAGCAACAAAG AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -21.1303 kcal/mol@LB@Unfolding Energy - mRNA 5.02575 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 1 -- 26@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 38 -- 44@LB@
63	0.002639	0.1417	b2751	cysN	-11.35	0.139541	181 -- 198	34 -- 49	sulfate adenylyltransferase subunit 1		_____________180________________199\n_____________\|__________________\|\n5'-AGU...AAAAA__G_______U_______AGAUG...GUG-3'\n______________AC_UCAGGGG_AUUUUUA\n______________\|\|_\|\|\|\|\|\|\|_\|\|\|\|::\|\n______________UG_AGUCCCC_UAAAGGU\n3'-UUU...CAAAG__________________ACGAA...GCA-5'\n_____________\|__________________\|\n_____________50_________________33\n	184 -- 190	41 -- 47	-17.8149 kcal/mol	4.64155 kcal/mol	1.82276 kcal/mol	180 199@LB@ \| \|@LB@5'-AGU...AAAAA G U AGAUG...GUG-3'@LB@ AC UCAGGGG AUUUUUA@LB@ \|\| \|\|\|\|\|\|\| \|\|\|\|::\|@LB@ UG AGUCCCC UAAAGGU@LB@3'-UUU...CAAAG ACGAA...GCA-5'@LB@ \| \|@LB@ 50 33@LB@@LB@@LB@Energy -11.35 kcal/mol@LB@Hybridization Energy -17.8149 kcal/mol@LB@Unfolding Energy - mRNA 4.64155 kcal/mol@LB@Unfolding Energy - sRNA 1.82276 kcal/mol@LB@@LB@Position - mRNA 181 -- 198@LB@Position - sRNA 34 -- 49@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 41 -- 47@LB@
62	0.002558	0.1417	b3579	yiaO	-11.31	0.142375	245 -- 258	62 -- 75	2 3-diketo-L-gulonate-binding periplasmic protein		_____________244____________259\n_____________\|______________\|\n5'-ACU...GGCCU________U_____CAUCU...AAA-3'\n______________GGCUGCAU_CAGCA\n______________\|:\|\|:\|\|:_\|\|\|\|\|\n______________CUGAUGUG_GUCGU\n3'-UUU...CGUUU________U_____UAAGC...GCA-5'\n_____________\|______________\|\n_____________76_____________61\n	246 -- 252	68 -- 74	-19.3904 kcal/mol	4.83304 kcal/mol	3.25167 kcal/mol	244 259@LB@ \| \|@LB@5'-ACU...GGCCU U CAUCU...AAA-3'@LB@ GGCUGCAU CAGCA@LB@ \|:\|\|:\|\|: \|\|\|\|\|@LB@ CUGAUGUG GUCGU@LB@3'-UUU...CGUUU U UAAGC...GCA-5'@LB@ \| \|@LB@ 76 61@LB@@LB@@LB@Energy -11.31 kcal/mol@LB@Hybridization Energy -19.3904 kcal/mol@LB@Unfolding Energy - mRNA 4.83304 kcal/mol@LB@Unfolding Energy - sRNA 3.25167 kcal/mol@LB@@LB@Position - mRNA 245 -- 258@LB@Position - sRNA 62 -- 75@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 68 -- 74@LB@
71	0.002886	0.1417	b2431	yfeX	-11.26	0.145044	235 -- 277	40 -- 74	porphyrinogen oxidase cytoplasmic		_____________234_________________________________________278\n_____________\|___________________________________________\|\n5'-AUU...AGAAC____C______________GAUCGAA__CAACGUGAA______AGUUG...CCA-3'\n______________AUUG_CGCGCGGCGAUUUG_______GC_________AGGGGA\n______________\|:\|:_\|:\|:\|:\|\|:\|\|\|:\|_______:\|_________\|\|\|\|\|\|\n______________UGAU_GUGUGUCGUUAAGC_______UG_________UCCCCU\n3'-UUU...GUUUC___________________AACAAAG__AG_____________AAAGG...GCA-5'\n_____________\|___________________________________________\|\n_____________75__________________________________________39\n	246 -- 252	58 -- 64	-30.0675 kcal/mol	14.0273 kcal/mol	4.77707 kcal/mol	234 278@LB@ \| \|@LB@5'-AUU...AGAAC C GAUCGAA CAACGUGAA AGUUG...CCA-3'@LB@ AUUG CGCGCGGCGAUUUG GC AGGGGA@LB@ \|:\|: \|:\|:\|:\|\|:\|\|\|:\| :\| \|\|\|\|\|\|@LB@ UGAU GUGUGUCGUUAAGC UG UCCCCU@LB@3'-UUU...GUUUC AACAAAG AG AAAGG...GCA-5'@LB@ \| \|@LB@ 75 39@LB@@LB@@LB@Energy -11.26 kcal/mol@LB@Hybridization Energy -30.0675 kcal/mol@LB@Unfolding Energy - mRNA 14.0273 kcal/mol@LB@Unfolding Energy - sRNA 4.77707 kcal/mol@LB@@LB@Position - mRNA 235 -- 277@LB@Position - sRNA 40 -- 74@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 58 -- 64@LB@
160	0.007693	0.1668	b1671	ydhX	-11.21	0.148807	210 -- 235	39 -- 74	putative 4Fe-4S ferridoxin-type protein; FNR Nar NarP-regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex	b4072	_____________209__________________________________236\n_____________\|____________________________________\|\n5'-CAC...CCUUC___CG________________________U______GGGAA...AGG-3'\n______________ACU__ACGCA__AAUUUGU________UC_GGGGAU\n______________\|\|\|__\|\|:\|\|__\|\|\|\|:\|\|________\|\|_\|\|\|\|\|\|\n______________UGA__UGUGU__UUAAGCA________AG_CCCCUA\n3'-UUU...GUUUC___UG_____CG_______ACAAAGUG__U______AAGGU...GCA-5'\n_____________\|____________________________________\|\n_____________75___________________________________38\n	220 -- 226	56 -- 62	-21.5478 kcal/mol	5.56457 kcal/mol	4.77831 kcal/mol	209 236@LB@ \| \|@LB@5'-CAC...CCUUC CG U GGGAA...AGG-3'@LB@ ACU ACGCA AAUUUGU UC GGGGAU@LB@ \|\|\| \|\|:\|\| \|\|\|\|:\|\| \|\| \|\|\|\|\|\|@LB@ UGA UGUGU UUAAGCA AG CCCCUA@LB@3'-UUU...GUUUC UG CG ACAAAGUG U AAGGU...GCA-5'@LB@ \| \|@LB@ 75 38@LB@@LB@@LB@Energy -11.21 kcal/mol@LB@Hybridization Energy -21.5478 kcal/mol@LB@Unfolding Energy - mRNA 5.56457 kcal/mol@LB@Unfolding Energy - sRNA 4.77831 kcal/mol@LB@@LB@Position - mRNA 210 -- 235@LB@Position - sRNA 39 -- 74@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 56 -- 62@LB@
173	0.008582	0.1745	b0060	polB	-11.19	0.150164	8 -- 23	39 -- 51	DNA polymerase II		_____________7________________24\n_____________\|________________\|\n____5'-GAAGAGG_______UAU______AUUCU...AGG-3'\n______________UCGCUUA___GGGGAU\n______________\|\|:\|\|:\|___\|\|\|\|\|\|\n______________AGUGAGU___CCCCUA\n3'-UUU...AACAA________________AAGGU...GCA-5'\n_____________\|________________\|\n_____________52_______________38\n	8 -- 14	45 -- 51	-16.4647 kcal/mol	3.48224 kcal/mol	1.79807 kcal/mol	7 24@LB@ \| \|@LB@ 5'-GAAGAGG UAU AUUCU...AGG-3'@LB@ UCGCUUA GGGGAU@LB@ \|\|:\|\|:\| \|\|\|\|\|\|@LB@ AGUGAGU CCCCUA@LB@3'-UUU...AACAA AAGGU...GCA-5'@LB@ \| \|@LB@ 52 38@LB@@LB@@LB@Energy -11.19 kcal/mol@LB@Hybridization Energy -16.4647 kcal/mol@LB@Unfolding Energy - mRNA 3.48224 kcal/mol@LB@Unfolding Energy - sRNA 1.79807 kcal/mol@LB@@LB@Position - mRNA 8 -- 23@LB@Position - sRNA 39 -- 51@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 45 -- 51@LB@
111	0.004903	0.1545	b1735	chbR	-11.17	0.151186	109 -- 138	30 -- 50	repressor of chb operon for N N'-diacetylchitobiose utilization		_____________108____________________________139\n_____________\|______________________________\|\n5'-GAU...GUCCA________AUCAUUUAAU__CG________UGCGC...CUG-3'\n______________CGCUCAGG__________GA__UCCAUGCU\n______________\|:\|\|\|\|\|\|__________\|\|__\|\|\|\|\|\|\|\|\n______________GUGAGUCC__________CU__AGGUACGA\n3'-UUU...ACAAA________C___________AA________AUAUU...GCA-5'\n_____________\|______________________________\|\n_____________51_____________________________29\n	132 -- 138	30 -- 36	-23.5874 kcal/mol	7.28235 kcal/mol	5.13509 kcal/mol	108 139@LB@ \| \|@LB@5'-GAU...GUCCA AUCAUUUAAU CG UGCGC...CUG-3'@LB@ CGCUCAGG GA UCCAUGCU@LB@ \|:\|\|\|\|\|\| \|\| \|\|\|\|\|\|\|\|@LB@ GUGAGUCC CU AGGUACGA@LB@3'-UUU...ACAAA C AA AUAUU...GCA-5'@LB@ \| \|@LB@ 51 29@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -23.5874 kcal/mol@LB@Unfolding Energy - mRNA 7.28235 kcal/mol@LB@Unfolding Energy - sRNA 5.13509 kcal/mol@LB@@LB@Position - mRNA 109 -- 138@LB@Position - sRNA 30 -- 50@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 30 -- 36@LB@
199	0.01026	0.1776	b2324	mnmC	-11.15	0.152427	29 -- 45	45 -- 74	fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme		_____________28_____________________________46\n_____________\|______________________________\|\n5'-GCU...UAAUC______________________________AUACC...ACG-3'\n______________ACUGCAC_____________GUUUCAUUCA\n______________\|\|\|:\|\|\|_____________\|\|\|\|\|\|:\|\|\|\n______________UGAUGUG_____________CAAAGUGAGU\n3'-UUU...GUUUC_______UGUCGUUAAGCAA__________CCCCU...GCA-5'\n_____________\|______________________________\|\n_____________75_____________________________44\n	39 -- 45	45 -- 51	-17.885 kcal/mol	2.99426 kcal/mol	3.74056 kcal/mol	28 46@LB@ \| \|@LB@5'-GCU...UAAUC AUACC...ACG-3'@LB@ ACUGCAC GUUUCAUUCA@LB@ \|\|\|:\|\|\| \|\|\|\|\|\|:\|\|\|@LB@ UGAUGUG CAAAGUGAGU@LB@3'-UUU...GUUUC UGUCGUUAAGCAA CCCCU...GCA-5'@LB@ \| \|@LB@ 75 44@LB@@LB@@LB@Energy -11.15 kcal/mol@LB@Hybridization Energy -17.885 kcal/mol@LB@Unfolding Energy - mRNA 2.99426 kcal/mol@LB@Unfolding Energy - sRNA 3.74056 kcal/mol@LB@@LB@Position - mRNA 29 -- 45@LB@Position - sRNA 45 -- 74@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 45 -- 51@LB@
38	0.0006674	0.0618	b2069	yegD	-11.14	0.153468	57 -- 84	48 -- 76	Hsp70 chaperone family protein		_____________56____________________________85\n_____________\|_____________________________\|\n5'-CAC...AGUUC___G_______C___UC__CUUA______CCGCU...UGC-3'\n______________AGG_UAUACAU_GCA__UC_____UUCAU\n______________\|\|:_\|\|:\|\|\|:_\|\|\|__\|\|_____\|\|\|\|:\n______________UCU_AUGUGUG_CGU__AG_____AAGUG\n3'-UUU...CCGUU___G_______U___UA__CAACA_____AGUCC...GCA-5'\n_____________\|_____________________________\|\n_____________77____________________________47\n	61 -- 67	66 -- 72	-18.1339 kcal/mol	3.31217 kcal/mol	3.68714 kcal/mol	56 85@LB@ \| \|@LB@5'-CAC...AGUUC G C UC CUUA CCGCU...UGC-3'@LB@ AGG UAUACAU GCA UC UUCAU@LB@ \|\|: \|\|:\|\|\|: \|\|\| \|\| \|\|\|\|:@LB@ UCU AUGUGUG CGU AG AAGUG@LB@3'-UUU...CCGUU G U UA CAACA AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -11.14 kcal/mol@LB@Hybridization Energy -18.1339 kcal/mol@LB@Unfolding Energy - mRNA 3.31217 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 57 -- 84@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 66 -- 72@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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