Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
59	0.002304	0.1363	b2508	guaB	-11.36	0.139274	1 -- 26	38 -- 71	IMP dehydrogenase	______________1__________________________________27\n______________\|__________________________________\|\n___________5'-__UA_______AA____________G_________GAGAA...AGC-3'\n______________AC__ACGGUAA___________AUU_CAGGGGAUU\n______________\|\|__\|\|:\|:\|\|___________\|:\|_\|\|\|\|\|\|\|\|\|\n______________UG__UGUCGUU___________UGA_GUCCCCUAA\n3'-UUU...UCUGA__UG_______AAGCAACAAAG_____________AGGUA...GCA-5'\n_____________\|___________________________________\|\n_____________72__________________________________37\n	20 -- 26	38 -- 44	-21.1303 kcal/mol	5.02575 kcal/mol	4.74986 kcal/mol	1 27@LB@ \| \|@LB@ 5'- UA AA G GAGAA...AGC-3'@LB@ AC ACGGUAA AUU CAGGGGAUU@LB@ \|\| \|\|:\|:\|\| \|:\| \|\|\|\|\|\|\|\|\|@LB@ UG UGUCGUU UGA GUCCCCUAA@LB@3'-UUU...UCUGA UG AAGCAACAAAG AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -21.1303 kcal/mol@LB@Unfolding Energy - mRNA 5.02575 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 1 -- 26@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 38 -- 44@LB@
87	0.003844	0.1538	b3124	garK	-12.12	0.097757	2 -- 34	58 -- 88	glycerate kinase I	_____________1_________________________________35\n_____________\|_________________________________\|\n__________5'-U_______AU_____C______A____A______AGGAA...AUG-3'\n______________GGAGAUG__GCAGG_ACUGCG_GCAG_UGGUUU\n______________\|\|\|\|\|\|\|__\|\|\|::_\|\|\|:\|:_:\|\|\|_:::\|\|:\n______________CCUCUAC__CGUUU_UGAUGU_UGUC_GUUAAG\n3'-UUU...AGCAC_______C______C______G___________CAACA...GCA-5'\n_____________\|_________________________________\|\n_____________89________________________________57\n	2 -- 8	82 -- 88	-28.4892 kcal/mol	6.08629 kcal/mol	10.2803 kcal/mol	1 35@LB@ \| \|@LB@ 5'-U AU C A A AGGAA...AUG-3'@LB@ GGAGAUG GCAGG ACUGCG GCAG UGGUUU@LB@ \|\|\|\|\|\|\| \|\|\|:: \|\|\|:\|: :\|\|\| :::\|\|:@LB@ CCUCUAC CGUUU UGAUGU UGUC GUUAAG@LB@3'-UUU...AGCAC C C G CAACA...GCA-5'@LB@ \| \|@LB@ 89 57@LB@@LB@@LB@Energy -12.12 kcal/mol@LB@Hybridization Energy -28.4892 kcal/mol@LB@Unfolding Energy - mRNA 6.08629 kcal/mol@LB@Unfolding Energy - sRNA 10.2803 kcal/mol@LB@@LB@Position - mRNA 2 -- 34@LB@Position - sRNA 58 -- 88@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 82 -- 88@LB@
178	0.008884	0.1747	b0051	rsmA	-9.94	0.250586	2 -- 32	30 -- 58	16S rRNA m(6)A1518 m(6)A1519 dimethyltransferase SAM-dependent	_____________1_______________________________33\n_____________\|_______________________________\|\n__________5'-G__A___AC___GA______UC__C_______AAAAU...UUA-3'\n______________CG_UGU__CAC__UCAGGG__UU_CCGUGCU\n______________\|\|_\|\|\|__\|\|\|__\|\|\|\|\|\|__\|\|_\|\|:\|\|\|\|\n______________GC_ACA__GUG__AGUCCC__AA_GGUACGA\n3'-UUU...GUUAA__A___AA___________CU__A_______AUAUU...GCA-5'\n_____________\|_______________________________\|\n_____________59______________________________29\n	26 -- 32	30 -- 36	-26.3106 kcal/mol	10.5715 kcal/mol	5.79877 kcal/mol	1 33@LB@ \| \|@LB@ 5'-G A AC GA UC C AAAAU...UUA-3'@LB@ CG UGU CAC UCAGGG UU CCGUGCU@LB@ \|\| \|\|\| \|\|\| \|\|\|\|\|\| \|\| \|\|:\|\|\|\|@LB@ GC ACA GUG AGUCCC AA GGUACGA@LB@3'-UUU...GUUAA A AA CU A AUAUU...GCA-5'@LB@ \| \|@LB@ 59 29@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -26.3106 kcal/mol@LB@Unfolding Energy - mRNA 10.5715 kcal/mol@LB@Unfolding Energy - sRNA 5.79877 kcal/mol@LB@@LB@Position - mRNA 2 -- 32@LB@Position - sRNA 30 -- 58@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 30 -- 36@LB@
188	0.009562	0.1776	b0147	ligT	-10.24	0.223023	2 -- 33	35 -- 66	2'-5' RNA ligase	_____________1_________________________________34\n_____________\|_________________________________\|\n__________5'-C________GCAGCGG___GC____UU_______CGGCC...CGG-3'\n______________CAGCAGUU________CA__CAGG__GAUUUCC\n______________\|\|\|\|\|:\|\|________\|\|__\|\|\|\|__\|\|\|\|\|\|\|\n______________GUCGUUAA________GU__GUCC__CUAAAGG\n3'-UUU...UGUGU________GCAACAAA__GA____C________UACGA...GCA-5'\n_____________\|_________________________________\|\n_____________67________________________________34\n	27 -- 33	35 -- 41	-25.6384 kcal/mol	10.64 kcal/mol	4.7609 kcal/mol	1 34@LB@ \| \|@LB@ 5'-C GCAGCGG GC UU CGGCC...CGG-3'@LB@ CAGCAGUU CA CAGG GAUUUCC@LB@ \|\|\|\|\|:\|\| \|\| \|\|\|\| \|\|\|\|\|\|\|@LB@ GUCGUUAA GU GUCC CUAAAGG@LB@3'-UUU...UGUGU GCAACAAA GA C UACGA...GCA-5'@LB@ \| \|@LB@ 67 34@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -25.6384 kcal/mol@LB@Unfolding Energy - mRNA 10.64 kcal/mol@LB@Unfolding Energy - sRNA 4.7609 kcal/mol@LB@@LB@Position - mRNA 2 -- 33@LB@Position - sRNA 35 -- 66@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 35 -- 41@LB@
50	0.001668	0.1153	b2821	ptrA	-10.19	0.227412	3 -- 12	46 -- 55	protease III	_____________2__________13\n_____________\|__________\|\n_________5'-GC__________UAAUC...CCA-3'\n______________UGUUUCGCUU\n______________\|\|\|\|\|\|:\|\|:\n______________ACAAAGUGAG\n3'-UUU...AAGCA__________UCCCC...GCA-5'\n_____________\|__________\|\n_____________56_________45\n	6 -- 12	46 -- 52	-11.8599 kcal/mol	0.772948 kcal/mol	0.89782 kcal/mol	2 13@LB@ \| \|@LB@ 5'-GC UAAUC...CCA-3'@LB@ UGUUUCGCUU@LB@ \|\|\|\|\|\|:\|\|:@LB@ ACAAAGUGAG@LB@3'-UUU...AAGCA UCCCC...GCA-5'@LB@ \| \|@LB@ 56 45@LB@@LB@@LB@Energy -10.19 kcal/mol@LB@Hybridization Energy -11.8599 kcal/mol@LB@Unfolding Energy - mRNA 0.772948 kcal/mol@LB@Unfolding Energy - sRNA 0.89782 kcal/mol@LB@@LB@Position - mRNA 3 -- 12@LB@Position - sRNA 46 -- 55@LB@Position Seed - mRNA 6 -- 12@LB@Position Seed - sRNA 46 -- 52@LB@
127	0.005686	0.1575	b1176	minC	-17.54	0.003902	3 -- 40	35 -- 74	inhibitor of FtsZ ring polymerization	_____________2________________________________________41\n_____________\|________________________________________\|\n_________5'-AA_G_____________GG____A__G__A_____U______UAAGC...AAG-3'\n______________A_UACG_ACGGCAAU__GUUG_UU_AC__AGGG_AUUUUU\n______________\|_\|\|\|:_\|\|:\|\|\|\|\|__\|\|\|\|_\|\|_\|\|__\|\|\|\|_\|\|\|\|::\n______________U_AUGU_UGUCGUUA__CAAC_AA_UG__UCCC_UAAAGG\n3'-UUU...GUUUC_G____G________AG____A__G__AG____C______UACGA...GCA-5'\n_____________\|________________________________________\|\n_____________75_______________________________________34\n	10 -- 16	60 -- 66	-29.7113 kcal/mol	7.37167 kcal/mol	4.80541 kcal/mol	2 41@LB@ \| \|@LB@ 5'-AA G GG A G A U UAAGC...AAG-3'@LB@ A UACG ACGGCAAU GUUG UU AC AGGG AUUUUU@LB@ \| \|\|\|: \|\|:\|\|\|\|\| \|\|\|\| \|\| \|\| \|\|\|\| \|\|\|\|::@LB@ U AUGU UGUCGUUA CAAC AA UG UCCC UAAAGG@LB@3'-UUU...GUUUC G G AG A G AG C UACGA...GCA-5'@LB@ \| \|@LB@ 75 34@LB@@LB@@LB@Energy -17.54 kcal/mol@LB@Hybridization Energy -29.7113 kcal/mol@LB@Unfolding Energy - mRNA 7.37167 kcal/mol@LB@Unfolding Energy - sRNA 4.80541 kcal/mol@LB@@LB@Position - mRNA 3 -- 40@LB@Position - sRNA 35 -- 74@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 60 -- 66@LB@
138	0.006392	0.1605	b0345	lacI	-7.62	0.533808	7 -- 17	61 -- 71	lactose-inducible lac operon transcriptional repressor	_____________6___________18\n_____________\|___________\|\n_____5'-CAUUGA___G_______AAAAG...AAA-3'\n______________ACA_GCAGCGG\n______________\|\|\|_:\|\|\|\|::\n______________UGU_UGUCGUU\n3'-UUU...UCUGA___G_______AAGCA...GCA-5'\n_____________\|___________\|\n_____________72__________60\n	11 -- 17	61 -- 67	-13.1569 kcal/mol	2.35774 kcal/mol	3.17655 kcal/mol	6 18@LB@ \| \|@LB@ 5'-CAUUGA G AAAAG...AAA-3'@LB@ ACA GCAGCGG@LB@ \|\|\| :\|\|\|\|::@LB@ UGU UGUCGUU@LB@3'-UUU...UCUGA G AAGCA...GCA-5'@LB@ \| \|@LB@ 72 60@LB@@LB@@LB@Energy -7.62 kcal/mol@LB@Hybridization Energy -13.1569 kcal/mol@LB@Unfolding Energy - mRNA 2.35774 kcal/mol@LB@Unfolding Energy - sRNA 3.17655 kcal/mol@LB@@LB@Position - mRNA 7 -- 17@LB@Position - sRNA 61 -- 71@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 61 -- 67@LB@
197	0.01006	0.1776	b0069	sgrR	-8.41	0.425671	7 -- 22	58 -- 74	transcriptional DNA-binding transcriptional activator of sgrS sRNA	_____________6_________________23\n_____________\|_________________\|\n_____5'-CAUCCC____________CC___AAGCA...UGA-3'\n______________ACUGCAU_CAGU__UUC\n______________\|\|\|:\|\|:_\|\|\|:__\|\|\|\n______________UGAUGUG_GUCG__AAG\n3'-UUU...GUUUC_______U____UU___CAACA...GCA-5'\n_____________\|_________________\|\n_____________75________________57\n	7 -- 13	68 -- 74	-13.3461 kcal/mol	1.63335 kcal/mol	3.3008 kcal/mol	6 23@LB@ \| \|@LB@ 5'-CAUCCC CC AAGCA...UGA-3'@LB@ ACUGCAU CAGU UUC@LB@ \|\|\|:\|\|: \|\|\|: \|\|\|@LB@ UGAUGUG GUCG AAG@LB@3'-UUU...GUUUC U UU CAACA...GCA-5'@LB@ \| \|@LB@ 75 57@LB@@LB@@LB@Energy -8.41 kcal/mol@LB@Hybridization Energy -13.3461 kcal/mol@LB@Unfolding Energy - mRNA 1.63335 kcal/mol@LB@Unfolding Energy - sRNA 3.3008 kcal/mol@LB@@LB@Position - mRNA 7 -- 22@LB@Position - sRNA 58 -- 74@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 68 -- 74@LB@
99	0.004523	0.1538	b1516	lsrB	-10.33	0.215118	8 -- 28	39 -- 59	autoinducer 2-binding protein	_____________7______________________29\n_____________\|______________________\|\n____5'-ACCGCCA___CG___________U_____AUUUG...UUC-3'\n______________UUG__GUUUUAUU_AG_GGGAU\n______________\|:\|__\|\|\|\|:\|:\|_\|\|_\|\|\|\|\|\n______________AGC__CAAAGUGA_UC_CCCUA\n3'-UUU...CGUUA___AA________G________AAGGU...GCA-5'\n_____________\|______________________\|\n_____________60_____________________38\n	14 -- 20	47 -- 53	-16.4048 kcal/mol	3.58309 kcal/mol	2.49358 kcal/mol	7 29@LB@ \| \|@LB@ 5'-ACCGCCA CG U AUUUG...UUC-3'@LB@ UUG GUUUUAUU AG GGGAU@LB@ \|:\| \|\|\|\|:\|:\| \|\| \|\|\|\|\|@LB@ AGC CAAAGUGA UC CCCUA@LB@3'-UUU...CGUUA AA G AAGGU...GCA-5'@LB@ \| \|@LB@ 60 38@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -16.4048 kcal/mol@LB@Unfolding Energy - mRNA 3.58309 kcal/mol@LB@Unfolding Energy - sRNA 2.49358 kcal/mol@LB@@LB@Position - mRNA 8 -- 28@LB@Position - sRNA 39 -- 59@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 47 -- 53@LB@
103	0.004595	0.1538	b3554	yiaF	-10.61	0.191588	8 -- 38	46 -- 75	barrier effect co-colonization resistance factor; DUF3053 family lipoprotein	_____________7_________________________________39\n_____________\|_________________________________\|\n____5'-AAUCUCU_______AAAAA_________GCACUA______UUUAA...AGC-3'\n______________GGCUACA_____GGCAA__CG______UCAUUU\n______________\|:\|\|\|\|\|_____:\|\|\|\|__\|\|______\|\|\|:\|:\n______________CUGAUGU_____UCGUU__GC______AGUGAG\n3'-UUU...CGUUU_______GUG_______AA__AACAA_______UCCCC...GCA-5'\n_____________\|_________________________________\|\n_____________76________________________________45\n	8 -- 14	69 -- 75	-17.9003 kcal/mol	3.5618 kcal/mol	3.72616 kcal/mol	7 39@LB@ \| \|@LB@ 5'-AAUCUCU AAAAA GCACUA UUUAA...AGC-3'@LB@ GGCUACA GGCAA CG UCAUUU@LB@ \|:\|\|\|\|\| :\|\|\|\| \|\| \|\|\|:\|:@LB@ CUGAUGU UCGUU GC AGUGAG@LB@3'-UUU...CGUUU GUG AA AACAA UCCCC...GCA-5'@LB@ \| \|@LB@ 76 45@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -17.9003 kcal/mol@LB@Unfolding Energy - mRNA 3.5618 kcal/mol@LB@Unfolding Energy - sRNA 3.72616 kcal/mol@LB@@LB@Position - mRNA 8 -- 38@LB@Position - sRNA 46 -- 75@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 69 -- 75@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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