Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
56	0.001982	0.1223	b2304	yfcH	-16.49	0.008143	193 -- 225	40 -- 77	putative NAD-dependent nucleotide-sugar epimerase	_____________192_________________________________________226\n_____________\|___________________________________________\|\n5'-AAA...AUCGG________AUGA__________________________A____CAGGA...ACC-3'\n______________GAGGCUGC____ACAUAGUGA_________UCAC_CGG_GGGA\n______________:\|\|:\|\|:\|____\|\|\|:\|\|::\|_________\|\|\|\|_\|:\|_\|\|\|\|\n______________UUCUGAUG____UGUGUCGUU_________AGUG_GUC_CCCU\n3'-UUU...CCCGU_____________________AAGCAACAA____A________AAAGG...GCA-5'\n_____________\|___________________________________________\|\n_____________78__________________________________________39\n	206 -- 212	62 -- 68	-29.217 kcal/mol	7.9505 kcal/mol	4.77871 kcal/mol	192 226@LB@ \| \|@LB@5'-AAA...AUCGG AUGA A CAGGA...ACC-3'@LB@ GAGGCUGC ACAUAGUGA UCAC CGG GGGA@LB@ :\|\|:\|\|:\| \|\|\|:\|\|::\| \|\|\|\| \|:\| \|\|\|\|@LB@ UUCUGAUG UGUGUCGUU AGUG GUC CCCU@LB@3'-UUU...CCCGU AAGCAACAA A AAAGG...GCA-5'@LB@ \| \|@LB@ 78 39@LB@@LB@@LB@Energy -16.49 kcal/mol@LB@Hybridization Energy -29.217 kcal/mol@LB@Unfolding Energy - mRNA 7.9505 kcal/mol@LB@Unfolding Energy - sRNA 4.77871 kcal/mol@LB@@LB@Position - mRNA 193 -- 225@LB@Position - sRNA 40 -- 77@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 62 -- 68@LB@
69	0.002809	0.1417	b1925	fliS	-16.42	0.008504	133 -- 153	57 -- 74	flagellar protein potentiates polymerization	_____________132___________________154\n_____________\|_____________________\|\n5'-UUA...CAGCA_____UUA_____________AAAAC...AUG-3'\n______________GCUAC___ACGCAGCAGUUCG\n______________:\|\|\|\|___\|\|:\|\|\|\|\|:\|\|\|\|\n______________UGAUG___UGUGUCGUUAAGC\n3'-UUU...GUUUC_____________________AACAA...GCA-5'\n_____________\|_____________________\|\n_____________75____________________56\n	147 -- 153	57 -- 63	-25.0139 kcal/mol	5.019 kcal/mol	3.57148 kcal/mol	132 154@LB@ \| \|@LB@5'-UUA...CAGCA UUA AAAAC...AUG-3'@LB@ GCUAC ACGCAGCAGUUCG@LB@ :\|\|\|\| \|\|:\|\|\|\|\|:\|\|\|\|@LB@ UGAUG UGUGUCGUUAAGC@LB@3'-UUU...GUUUC AACAA...GCA-5'@LB@ \| \|@LB@ 75 56@LB@@LB@@LB@Energy -16.42 kcal/mol@LB@Hybridization Energy -25.0139 kcal/mol@LB@Unfolding Energy - mRNA 5.019 kcal/mol@LB@Unfolding Energy - sRNA 3.57148 kcal/mol@LB@@LB@Position - mRNA 133 -- 153@LB@Position - sRNA 57 -- 74@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 57 -- 63@LB@
156	0.007591	0.1668	b1044	ymdA	-16.39	0.008687	226 -- 252	46 -- 73	uncharacterized protein	_____________225__________________________253\n_____________\|____________________________\|\n5'-UGA...CUCUC______U____________U________UUAUU...UUU-3'\n______________UUAUGC_CGGCAGUUUGUU_UUUC_CUU\n______________:\|\|::\|_\|:\|\|\|:\|\|:\|\|\|_\|\|\|\|_\|\|:\n______________GAUGUG_GUCGUUAAGCAA_AAAG_GAG\n3'-UUU...UUUCU______U____________C____U___UCCCC...GCA-5'\n_____________\|____________________________\|\n_____________74___________________________45\n	236 -- 242	57 -- 63	-24.6333 kcal/mol	4.51706 kcal/mol	3.72492 kcal/mol	225 253@LB@ \| \|@LB@5'-UGA...CUCUC U U UUAUU...UUU-3'@LB@ UUAUGC CGGCAGUUUGUU UUUC CUU@LB@ :\|\|::\| \|:\|\|\|:\|\|:\|\|\| \|\|\|\| \|\|:@LB@ GAUGUG GUCGUUAAGCAA AAAG GAG@LB@3'-UUU...UUUCU U C U UCCCC...GCA-5'@LB@ \| \|@LB@ 74 45@LB@@LB@@LB@Energy -16.39 kcal/mol@LB@Hybridization Energy -24.6333 kcal/mol@LB@Unfolding Energy - mRNA 4.51706 kcal/mol@LB@Unfolding Energy - sRNA 3.72492 kcal/mol@LB@@LB@Position - mRNA 226 -- 252@LB@Position - sRNA 46 -- 73@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 57 -- 63@LB@
30	0.0004324	0.05073	b3364	tsgA	-16.38	0.008733	203 -- 236	38 -- 75	putative transporter	_____________202_____________________________________237\n_____________\|_______________________________________\|\n5'-CCA...AUCAU___________________CAUCAA______CAU_____AGCUU...AUA-3'\n______________GACUA___ACAGCAAU_CG_______GCUCA___GGAUU\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\|_\|\|_______:\|\|\|\|___\|\|\|\|\|\n______________CUGAU___UGUCGUUA_GC_______UGAGU___CCUAA\n3'-UUU...CGUUU_____GUG________A__AACAAAG_____CC______AGGUA...GCA-5'\n_____________\|_______________________________________\|\n_____________76______________________________________37\n	209 -- 215	60 -- 66	-26.4683 kcal/mol	5.30565 kcal/mol	4.77946 kcal/mol	202 237@LB@ \| \|@LB@5'-CCA...AUCAU CAUCAA CAU AGCUU...AUA-3'@LB@ GACUA ACAGCAAU CG GCUCA GGAUU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\| \|\| :\|\|\|\| \|\|\|\|\|@LB@ CUGAU UGUCGUUA GC UGAGU CCUAA@LB@3'-UUU...CGUUU GUG A AACAAAG CC AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -16.38 kcal/mol@LB@Hybridization Energy -26.4683 kcal/mol@LB@Unfolding Energy - mRNA 5.30565 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 203 -- 236@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 60 -- 66@LB@
81	0.003429	0.1462	b0413	nrdR	-16.32	0.009130	136 -- 177	33 -- 64	Nrd regulon repressor	_____________135_________________________________________178\n_____________\|___________________________________________\|\n5'-AGG...GUUGC__C_________A___UCAG_______UAAAAUA_________UAACC...UGU-3'\n______________GC__UUUGUUGU_UCG____UUCAGGG_______GAUUUCCGU\n______________\|\|__\|\|:\|\|\|\|\|_\|\|:____:\|\|\|\|\|\|_______\|\|\|\|\|\|\|:\|\n______________CG__AAGCAACA_AGU____GAGUCCC_______CUAAAGGUA\n3'-UUU...UGUGU__UU________A______________________________CGAAU...GCA-5'\n_____________\|___________________________________________\|\n_____________65__________________________________________32\n	171 -- 177	33 -- 39	-28.4719 kcal/mol	7.3911 kcal/mol	4.76435 kcal/mol	135 178@LB@ \| \|@LB@5'-AGG...GUUGC C A UCAG UAAAAUA UAACC...UGU-3'@LB@ GC UUUGUUGU UCG UUCAGGG GAUUUCCGU@LB@ \|\| \|\|:\|\|\|\|\| \|\|: :\|\|\|\|\|\| \|\|\|\|\|\|\|:\|@LB@ CG AAGCAACA AGU GAGUCCC CUAAAGGUA@LB@3'-UUU...UGUGU UU A CGAAU...GCA-5'@LB@ \| \|@LB@ 65 32@LB@@LB@@LB@Energy -16.32 kcal/mol@LB@Hybridization Energy -28.4719 kcal/mol@LB@Unfolding Energy - mRNA 7.3911 kcal/mol@LB@Unfolding Energy - sRNA 4.76435 kcal/mol@LB@@LB@Position - mRNA 136 -- 177@LB@Position - sRNA 33 -- 64@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 33 -- 39@LB@
102	0.004593	0.1538	b0343	lacY	-16.22	0.009713	12 -- 55	39 -- 78	lactose permease	_____________11___________________________________________56\n_____________\|____________________________________________\|\n5'-UGC...ACGCG_____AG_________UG___A___ACG_____CA_AU______UGGUG...GGC-3'\n______________GAAGA__GCACAUGGC__AAU_UCG___GUUUC__U__GGGGAU\n______________:\|\|\|\|__:\|\|\|\|::\|\|__\|\|\|_\|\|\|___\|\|\|\|\|__\|__\|\|\|\|\|\|\n______________UUUCU__UGUGUGUCG__UUA_AGC___CAAAG__A__CCCCUA\n3'-UUU...ACCCG_____GA__________________AA______UG_GU______AAGGU...GCA-5'\n_____________\|____________________________________________\|\n_____________79___________________________________________38\n	21 -- 27	63 -- 69	-31.4377 kcal/mol	10.4352 kcal/mol	4.77966 kcal/mol	11 56@LB@ \| \|@LB@5'-UGC...ACGCG AG UG A ACG CA AU UGGUG...GGC-3'@LB@ GAAGA GCACAUGGC AAU UCG GUUUC U GGGGAU@LB@ :\|\|\|\| :\|\|\|\|::\|\| \|\|\| \|\|\| \|\|\|\|\| \| \|\|\|\|\|\|@LB@ UUUCU UGUGUGUCG UUA AGC CAAAG A CCCCUA@LB@3'-UUU...ACCCG GA AA UG GU AAGGU...GCA-5'@LB@ \| \|@LB@ 79 38@LB@@LB@@LB@Energy -16.22 kcal/mol@LB@Hybridization Energy -31.4377 kcal/mol@LB@Unfolding Energy - mRNA 10.4352 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 12 -- 55@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 63 -- 69@LB@
39	0.0006857	0.06187	b1305	pspB	-16.08	0.010660	268 -- 296	48 -- 75	psp operon transcription co-activator	_____________267____________________________297\n_____________\|______________________________\|\n5'-AUU...GUUAU_______U______________CUGG____AGUG-3'\n______________GGCUGCA_UACAGCAAU_CGUU____UCGC\n______________\|:\|\|:\|\|_:\|\|\|\|\|\|\|\|_\|\|\|\|____\|\|:\|\n______________CUGAUGU_GUGUCGUUA_GCAA____AGUG\n3'-UUU...CGUUU_________________A____CAA_____AGUCC...GCA-5'\n_____________\|______________________________\|\n_____________76_____________________________47\n	278 -- 284	60 -- 66	-29.5086 kcal/mol	9.74074 kcal/mol	3.68696 kcal/mol	267 297@LB@ \| \|@LB@5'-AUU...GUUAU U CUGG AGUG-3'@LB@ GGCUGCA UACAGCAAU CGUU UCGC@LB@ \|:\|\|:\|\| :\|\|\|\|\|\|\|\| \|\|\|\| \|\|:\|@LB@ CUGAUGU GUGUCGUUA GCAA AGUG@LB@3'-UUU...CGUUU A CAA AGUCC...GCA-5'@LB@ \| \|@LB@ 76 47@LB@@LB@@LB@Energy -16.08 kcal/mol@LB@Hybridization Energy -29.5086 kcal/mol@LB@Unfolding Energy - mRNA 9.74074 kcal/mol@LB@Unfolding Energy - sRNA 3.68696 kcal/mol@LB@@LB@Position - mRNA 268 -- 296@LB@Position - sRNA 48 -- 75@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 60 -- 66@LB@
83	0.003448	0.1462	b4231	yjfF	-16.06	0.010834	60 -- 72	39 -- 51	putative sugar ABC transporter permease	_____________59____________73\n_____________\|_____________\|\n5'-CGG...GCUGA_____________GAACA...GAG-3'\n______________UUAUUCAGGGGAU\n______________\|:\|:\|\|\|\|\|\|\|\|\|\n______________AGUGAGUCCCCUA\n3'-UUU...AACAA_____________AAGGU...GCA-5'\n_____________\|_____________\|\n_____________52____________38\n	66 -- 72	39 -- 45	-20.8743 kcal/mol	3.02031 kcal/mol	1.79807 kcal/mol	59 73@LB@ \| \|@LB@5'-CGG...GCUGA GAACA...GAG-3'@LB@ UUAUUCAGGGGAU@LB@ \|:\|:\|\|\|\|\|\|\|\|\|@LB@ AGUGAGUCCCCUA@LB@3'-UUU...AACAA AAGGU...GCA-5'@LB@ \| \|@LB@ 52 38@LB@@LB@@LB@Energy -16.06 kcal/mol@LB@Hybridization Energy -20.8743 kcal/mol@LB@Unfolding Energy - mRNA 3.02031 kcal/mol@LB@Unfolding Energy - sRNA 1.79807 kcal/mol@LB@@LB@Position - mRNA 60 -- 72@LB@Position - sRNA 39 -- 51@LB@Position Seed - mRNA 66 -- 72@LB@Position Seed - sRNA 39 -- 45@LB@
29	0.0004091	0.04965	b0958	sulA	-15.92	0.011809	212 -- 243	48 -- 76	SOS cell division inhibitor	_____________211______________________________244\n_____________\|________________________________\|\n5'-AUA...ACUUC____________UC__UC_____C___C____CCGCA...UCA-3'\n______________AGGCUAUGCACA__GU__UUCGU_GUU_UCAU\n______________\|\|:\|\|\|::\|\|\|\|__\|:__\|\|\|\|\|_\|\|\|_\|\|\|:\n______________UCUGAUGUGUGU__CG__AAGCA_CAA_AGUG\n3'-UUU...CCGUU________________UU_____A________AGUCC...GCA-5'\n_____________\|________________________________\|\n_____________77_______________________________47\n	217 -- 223	65 -- 71	-24.9625 kcal/mol	5.35271 kcal/mol	3.68714 kcal/mol	211 244@LB@ \| \|@LB@5'-AUA...ACUUC UC UC C C CCGCA...UCA-3'@LB@ AGGCUAUGCACA GU UUCGU GUU UCAU@LB@ \|\|:\|\|\|::\|\|\|\| \|: \|\|\|\|\| \|\|\| \|\|\|:@LB@ UCUGAUGUGUGU CG AAGCA CAA AGUG@LB@3'-UUU...CCGUU UU A AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -15.92 kcal/mol@LB@Hybridization Energy -24.9625 kcal/mol@LB@Unfolding Energy - mRNA 5.35271 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 212 -- 243@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 65 -- 71@LB@
16	0.00012	0.02638	b3089	sstT	-15.87	0.012233	203 -- 224	42 -- 75	sodium:serine/threonine symporter	_____________202________________________________225\n_____________\|__________________________________\|\n5'-CAC...AAAAU_________ACG______________________CUAUU...CAU-3'\n______________GACUACGCA______________UUCAC_CGGGG\n______________\|\|\|\|\|\|:\|\|______________\|\|\|\|\|_\|:\|\|\|\n______________CUGAUGUGU______________AAGUG_GUCCC\n3'-UUU...CGUUU_________GUCGUUAAGCAACA_____A_____CUAAA...GCA-5'\n_____________\|__________________________________\|\n_____________76_________________________________41\n	205 -- 211	67 -- 73	-24.6329 kcal/mol	4.00159 kcal/mol	4.76397 kcal/mol	202 225@LB@ \| \|@LB@5'-CAC...AAAAU ACG CUAUU...CAU-3'@LB@ GACUACGCA UUCAC CGGGG@LB@ \|\|\|\|\|\|:\|\| \|\|\|\|\| \|:\|\|\|@LB@ CUGAUGUGU AAGUG GUCCC@LB@3'-UUU...CGUUU GUCGUUAAGCAACA A CUAAA...GCA-5'@LB@ \| \|@LB@ 76 41@LB@@LB@@LB@Energy -15.87 kcal/mol@LB@Hybridization Energy -24.6329 kcal/mol@LB@Unfolding Energy - mRNA 4.00159 kcal/mol@LB@Unfolding Energy - sRNA 4.76397 kcal/mol@LB@@LB@Position - mRNA 203 -- 224@LB@Position - sRNA 42 -- 75@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 67 -- 73@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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