Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
189	0.009605	0.1776	b0927	ycbL	-15.21	0.018444	14 -- 51	33 -- 78	putative metal-binding enzyme	_____________13__________________________________________________52\n_____________\|___________________________________________________\|\n5'-GGA...GAAGA________U_____UAAAG__________________CGAU__________AUUGA...GCG-3'\n______________AAAG_CUA_CACAC________________GC_CAGG____GGAUUUCCAU\n______________\|\|\|\|_\|\|\|_\|\|\|\|\|________________:\|_\|\|\|\|____\|\|\|\|\|\|\|\|\|\|\n______________UUUC_GAU_GUGUG________________UG_GUCC____CCUAAAGGUA\n3'-UUU...ACCCG____U_________UCGUUAAGCAACAAAG__A__________________CGAAU...GCA-5'\n_____________\|___________________________________________________\|\n_____________79__________________________________________________32\n	45 -- 51	33 -- 39	-26.8189 kcal/mol	6.79395 kcal/mol	4.81602 kcal/mol	13 52@LB@ \| \|@LB@5'-GGA...GAAGA U UAAAG CGAU AUUGA...GCG-3'@LB@ AAAG CUA CACAC GC CAGG GGAUUUCCAU@LB@ \|\|\|\| \|\|\| \|\|\|\|\| :\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ UUUC GAU GUGUG UG GUCC CCUAAAGGUA@LB@3'-UUU...ACCCG U UCGUUAAGCAACAAAG A CGAAU...GCA-5'@LB@ \| \|@LB@ 79 32@LB@@LB@@LB@Energy -15.21 kcal/mol@LB@Hybridization Energy -26.8189 kcal/mol@LB@Unfolding Energy - mRNA 6.79395 kcal/mol@LB@Unfolding Energy - sRNA 4.81602 kcal/mol@LB@@LB@Position - mRNA 14 -- 51@LB@Position - sRNA 33 -- 78@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@
112	0.004986	0.1545	b0118	acnB	-15.18	0.018766	221 -- 251	38 -- 71	aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase	_____________220________________________________252\n_____________\|__________________________________\|\n5'-GAG...CGUAA_________UGAG___GC_______G________GCGCC...UGC-3'\n______________GCACGUAGC____CGU_____CGCU_AGGGGAUU\n______________:\|\|\|::\|\|\|____\|\|\|_____\|:\|\|_\|\|\|\|\|\|\|\|\n______________UGUGUGUCG____GCA_____GUGA_UCCCCUAA\n3'-UUU...UCUGA_________UUAA___ACAAA____G________AGGUA...GCA-5'\n_____________\|__________________________________\|\n_____________72_________________________________37\n	245 -- 251	38 -- 44	-33.2374 kcal/mol	13.3072 kcal/mol	4.74986 kcal/mol	220 252@LB@ \| \|@LB@5'-GAG...CGUAA UGAG GC G GCGCC...UGC-3'@LB@ GCACGUAGC CGU CGCU AGGGGAUU@LB@ :\|\|\|::\|\|\| \|\|\| \|:\|\| \|\|\|\|\|\|\|\|@LB@ UGUGUGUCG GCA GUGA UCCCCUAA@LB@3'-UUU...UCUGA UUAA ACAAA G AGGUA...GCA-5'@LB@ \| \|@LB@ 72 37@LB@@LB@@LB@Energy -15.18 kcal/mol@LB@Hybridization Energy -33.2374 kcal/mol@LB@Unfolding Energy - mRNA 13.3072 kcal/mol@LB@Unfolding Energy - sRNA 4.74986 kcal/mol@LB@@LB@Position - mRNA 221 -- 251@LB@Position - sRNA 38 -- 71@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 38 -- 44@LB@
169	0.008352	0.1723	b1222	narX	-15.18	0.018797	58 -- 74	61 -- 75	sensory histidine kinase in two-component regulatory system with NarL	_____________57________________75\n_____________\|_________________\|\n5'-AAA...AAAGC_____AC__________AUAAG...CUG-3'\n______________GACUA__CACACGGCAA\n______________\|\|\|\|\|__\|\|\|\|\|:\|\|\|\|\n______________CUGAU__GUGUGUCGUU\n3'-UUU...CGUUU_________________AAGCA...GCA-5'\n_____________\|_________________\|\n_____________76________________60\n	68 -- 74	61 -- 67	-20.5903 kcal/mol	2.1582 kcal/mol	3.25399 kcal/mol	57 75@LB@ \| \|@LB@5'-AAA...AAAGC AC AUAAG...CUG-3'@LB@ GACUA CACACGGCAA@LB@ \|\|\|\|\| \|\|\|\|\|:\|\|\|\|@LB@ CUGAU GUGUGUCGUU@LB@3'-UUU...CGUUU AAGCA...GCA-5'@LB@ \| \|@LB@ 76 60@LB@@LB@@LB@Energy -15.18 kcal/mol@LB@Hybridization Energy -20.5903 kcal/mol@LB@Unfolding Energy - mRNA 2.1582 kcal/mol@LB@Unfolding Energy - sRNA 3.25399 kcal/mol@LB@@LB@Position - mRNA 58 -- 74@LB@Position - sRNA 61 -- 75@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 61 -- 67@LB@
92	0.004122	0.1538	b3912	cpxR	-15.13	0.019325	106 -- 130	39 -- 76	response regulator in two-component regulatory system with CpxA	_____________105_____________________________________131\n_____________\|_______________________________________\|\n5'-UGA...GAUUC________A___U__________________________GUAAA...AUG-3'\n______________AGGCUGCA_ACA_GCG______________UCAGGGGGU\n______________\|\|:\|\|:\|\|_\|\|\|_\|\|:______________\|\|\|\|\|\|\|:\|\n______________UCUGAUGU_UGU_CGU______________AGUCCCCUA\n3'-UUU...CCGUU________G_______UAAGCAACAAAGUG_________AAGGU...GCA-5'\n_____________\|_______________________________________\|\n_____________77______________________________________38\n	124 -- 130	39 -- 45	-28.779 kcal/mol	8.86764 kcal/mol	4.77949 kcal/mol	105 131@LB@ \| \|@LB@5'-UGA...GAUUC A U GUAAA...AUG-3'@LB@ AGGCUGCA ACA GCG UCAGGGGGU@LB@ \|\|:\|\|:\|\| \|\|\| \|\|: \|\|\|\|\|\|\|:\|@LB@ UCUGAUGU UGU CGU AGUCCCCUA@LB@3'-UUU...CCGUU G UAAGCAACAAAGUG AAGGU...GCA-5'@LB@ \| \|@LB@ 77 38@LB@@LB@@LB@Energy -15.13 kcal/mol@LB@Hybridization Energy -28.779 kcal/mol@LB@Unfolding Energy - mRNA 8.86764 kcal/mol@LB@Unfolding Energy - sRNA 4.77949 kcal/mol@LB@@LB@Position - mRNA 106 -- 130@LB@Position - sRNA 39 -- 76@LB@Position Seed - mRNA 124 -- 130@LB@Position Seed - sRNA 39 -- 45@LB@
44	0.001243	0.09943	b3064	tsaD	-15.06	0.020167	98 -- 131	41 -- 82	tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein	_____________97___________________________________________132\n_____________\|____________________________________________\|\n5'-CAU...UAUAA_________G_______GA________________U________CGAAC...UGA-3'\n______________GGGUGAGGA_UAUACAC__GGC_________UUUC_C_CAGGGG\n______________\|\|\|::\|:\|\|_\|\|:\|\|\|\|__:\|\|_________\|\|\|\|_\|_\|\|\|\|\|\|\n______________CCCGUUUCU_AUGUGUG__UCG_________AAAG_G_GUCCCC\n3'-UUU...CUCUA_________G____________UUAAGCAAC____U_A______UAAAG...GCA-5'\n_____________\|____________________________________________\|\n_____________83___________________________________________40\n	108 -- 114	66 -- 72	-38.4936 kcal/mol	9.4641 kcal/mol	13.9685 kcal/mol	97 132@LB@ \| \|@LB@5'-CAU...UAUAA G GA U CGAAC...UGA-3'@LB@ GGGUGAGGA UAUACAC GGC UUUC C CAGGGG@LB@ \|\|\|::\|:\|\| \|\|:\|\|\|\| :\|\| \|\|\|\| \| \|\|\|\|\|\|@LB@ CCCGUUUCU AUGUGUG UCG AAAG G GUCCCC@LB@3'-UUU...CUCUA G UUAAGCAAC U A UAAAG...GCA-5'@LB@ \| \|@LB@ 83 40@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -38.4936 kcal/mol@LB@Unfolding Energy - mRNA 9.4641 kcal/mol@LB@Unfolding Energy - sRNA 13.9685 kcal/mol@LB@@LB@Position - mRNA 98 -- 131@LB@Position - sRNA 41 -- 82@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 66 -- 72@LB@
147	0.006785	0.1624	b1920	fliY	-15.06	0.020217	159 -- 198	40 -- 75	cystine transporter subunit	_____________158______________________________________199\n_____________\|________________________________________\|\n5'-CCA...ACAAU____C_____U____ACAGACAUAACAA_________U__AUAUG...UGC-3'\n______________GGCU_UACAC_GCAA_____________CAUUCGGGG_GA\n______________\|:\|\|_:\|\|\|\|_\|\|\|\|_____________\|\|:\|\|:\|\|\|_\|\|\n______________CUGA_GUGUG_CGUU_____________GUGAGUCCC_CU\n3'-UUU...CGUUU____U_____U____AAGCAACAAA_______________AAAGG...GCA-5'\n_____________\|________________________________________\|\n_____________76_______________________________________39\n	189 -- 195	42 -- 48	-27.6268 kcal/mol	7.79206 kcal/mol	4.77786 kcal/mol	158 199@LB@ \| \|@LB@5'-CCA...ACAAU C U ACAGACAUAACAA U AUAUG...UGC-3'@LB@ GGCU UACAC GCAA CAUUCGGGG GA@LB@ \|:\|\| :\|\|\|\| \|\|\|\| \|\|:\|\|:\|\|\| \|\|@LB@ CUGA GUGUG CGUU GUGAGUCCC CU@LB@3'-UUU...CGUUU U U AAGCAACAAA AAAGG...GCA-5'@LB@ \| \|@LB@ 76 39@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -27.6268 kcal/mol@LB@Unfolding Energy - mRNA 7.79206 kcal/mol@LB@Unfolding Energy - sRNA 4.77786 kcal/mol@LB@@LB@Position - mRNA 159 -- 198@LB@Position - sRNA 40 -- 75@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 42 -- 48@LB@
97	0.004509	0.1538	b0465	mscK	-15.00	0.020897	217 -- 248	49 -- 74	mechanosensitive channel protein intermediate conductance K+ regulated	_____________216______________________________249\n_____________\|________________________________\|\n5'-CGA...CCAGU______AACGAU_______U______U_____GCAUU...CGC-3'\n______________AUUACA______CACGGCA_UUUGUU_UUUCA\n______________\|:\|\|\|\|______\|\|\|:\|\|\|_\|\|:\|\|\|_\|\|\|\|\|\n______________UGAUGU______GUGUCGU_AAGCAA_AAAGU\n3'-UUU...GUUUC___________________U______C_____GAGUC...GCA-5'\n_____________\|________________________________\|\n_____________75_______________________________48\n	229 -- 235	62 -- 68	-21.8954 kcal/mol	3.22234 kcal/mol	3.67122 kcal/mol	216 249@LB@ \| \|@LB@5'-CGA...CCAGU AACGAU U U GCAUU...CGC-3'@LB@ AUUACA CACGGCA UUUGUU UUUCA@LB@ \|:\|\|\|\| \|\|\|:\|\|\| \|\|:\|\|\| \|\|\|\|\|@LB@ UGAUGU GUGUCGU AAGCAA AAAGU@LB@3'-UUU...GUUUC U C GAGUC...GCA-5'@LB@ \| \|@LB@ 75 48@LB@@LB@@LB@Energy -15.00 kcal/mol@LB@Hybridization Energy -21.8954 kcal/mol@LB@Unfolding Energy - mRNA 3.22234 kcal/mol@LB@Unfolding Energy - sRNA 3.67122 kcal/mol@LB@@LB@Position - mRNA 217 -- 248@LB@Position - sRNA 49 -- 74@LB@Position Seed - mRNA 229 -- 235@LB@Position Seed - sRNA 62 -- 68@LB@
129	0.005822	0.1577	b0713	ybgL	-14.99	0.021088	226 -- 247	61 -- 83	UPF0271 family protein	_____________225_____________________248\n_____________\|_______________________\|\n5'-GCC...CGAUC_______________________CGCAG...AUG-3'\n______________UGGGCGAAGGCUGCGC_CAGCGA\n______________\|\|\|\|\|:\|\|\|:\|\|:\|:\|_\|\|\|\|:\|\n______________ACCCGUUUCUGAUGUG_GUCGUU\n3'-UUU...CCUCU________________U______AAGCA...GCA-5'\n_____________\|_______________________\|\n_____________84______________________60\n	234 -- 240	69 -- 75	-36.9571 kcal/mol	11.7384 kcal/mol	10.2322 kcal/mol	225 248@LB@ \| \|@LB@5'-GCC...CGAUC CGCAG...AUG-3'@LB@ UGGGCGAAGGCUGCGC CAGCGA@LB@ \|\|\|\|\|:\|\|\|:\|\|:\|:\| \|\|\|\|:\|@LB@ ACCCGUUUCUGAUGUG GUCGUU@LB@3'-UUU...CCUCU U AAGCA...GCA-5'@LB@ \| \|@LB@ 84 60@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -36.9571 kcal/mol@LB@Unfolding Energy - mRNA 11.7384 kcal/mol@LB@Unfolding Energy - sRNA 10.2322 kcal/mol@LB@@LB@Position - mRNA 226 -- 247@LB@Position - sRNA 61 -- 83@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 69 -- 75@LB@
54	0.00187	0.1218	b3607	cysE	-14.87	0.022582	98 -- 129	42 -- 78	serine acetyltransferase	_____________97_________________________________________130\n_____________\|__________________________________________\|\n5'-CGG...CACGC______GA__________C_____________AACCC_____AACCU...CGA-3'\n______________AAGGAC___GCGCAGCAG__________CACU_____CAGGG\n______________\|\|:\|\|\|___:\|:\|\|\|\|\|:__________\|\|\|\|_____\|\|\|\|\|\n______________UUUCUG___UGUGUCGUU__________GUGA_____GUCCC\n3'-UUU...ACCCG______AUG_________AAGCAACAAA______________CUAAA...GCA-5'\n_____________\|__________________________________________\|\n_____________79_________________________________________41\n	108 -- 114	61 -- 67	-27.0645 kcal/mol	7.42824 kcal/mol	4.76487 kcal/mol	97 130@LB@ \| \|@LB@5'-CGG...CACGC GA C AACCC AACCU...CGA-3'@LB@ AAGGAC GCGCAGCAG CACU CAGGG@LB@ \|\|:\|\|\| :\|:\|\|\|\|\|: \|\|\|\| \|\|\|\|\|@LB@ UUUCUG UGUGUCGUU GUGA GUCCC@LB@3'-UUU...ACCCG AUG AAGCAACAAA CUAAA...GCA-5'@LB@ \| \|@LB@ 79 41@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -27.0645 kcal/mol@LB@Unfolding Energy - mRNA 7.42824 kcal/mol@LB@Unfolding Energy - sRNA 4.76487 kcal/mol@LB@@LB@Position - mRNA 98 -- 129@LB@Position - sRNA 42 -- 78@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 61 -- 67@LB@
22	0.0001899	0.02926	b2562	yfhL	-14.77	0.023959	28 -- 42	42 -- 56	putative 4Fe-4S cluster-containing protein	_____________27______________43\n_____________\|_______________\|\n5'-AGC...UCUGC____CA_________AAUGU...UUA-3'\n______________UUGU__CGCUCAGGG\n______________\|\|\|\|__\|:\|\|\|\|\|\|\|\n______________AACA__GUGAGUCCC\n3'-UUU...UAAGC____AA_________CUAAA...GCA-5'\n_____________\|_______________\|\n_____________57______________41\n	36 -- 42	42 -- 48	-19.6539 kcal/mol	2.98712 kcal/mol	1.89561 kcal/mol	27 43@LB@ \| \|@LB@5'-AGC...UCUGC CA AAUGU...UUA-3'@LB@ UUGU CGCUCAGGG@LB@ \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ AACA GUGAGUCCC@LB@3'-UUU...UAAGC AA CUAAA...GCA-5'@LB@ \| \|@LB@ 57 41@LB@@LB@@LB@Energy -14.77 kcal/mol@LB@Hybridization Energy -19.6539 kcal/mol@LB@Unfolding Energy - mRNA 2.98712 kcal/mol@LB@Unfolding Energy - sRNA 1.89561 kcal/mol@LB@@LB@Position - mRNA 28 -- 42@LB@Position - sRNA 42 -- 56@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 42 -- 48@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching