Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
149	0.005967	0.1406	b0472	recR	-13.66	0.002580	134 -- 160	3 -- 29	gap repair protein	_____________133_________________________161\n_____________\|___________________________\|\n5'-GUG...AACGC___A____AAAAUG_____________CCGGA...UUC-3'\n______________AGA_AGAA______GCCUCUGUAUCCU\n______________\|\|\|_\|\|\|\|______:\|\|\|\|\|\|\|\|:\|\|\|\n______________UCU_UCUU______UGGAGACAUGGGA\n3'-AUU...GACUU___A____GUAGAA_____________UG-5'\n_____________\|___________________________\|\n_____________30__________________________2\n	154 -- 160	3 -- 9	-27.6106 kcal/mol	5.92472 kcal/mol	8.05021 kcal/mol	133 161@LB@ \| \|@LB@5'-GUG...AACGC A AAAAUG CCGGA...UUC-3'@LB@ AGA AGAA GCCUCUGUAUCCU@LB@ \|\|\| \|\|\|\| :\|\|\|\|\|\|\|\|:\|\|\|@LB@ UCU UCUU UGGAGACAUGGGA@LB@3'-AUU...GACUU A GUAGAA UG-5'@LB@ \| \|@LB@ 30 2@LB@@LB@@LB@Energy -13.66 kcal/mol@LB@Hybridization Energy -27.6106 kcal/mol@LB@Unfolding Energy - mRNA 5.92472 kcal/mol@LB@Unfolding Energy - sRNA 8.05021 kcal/mol@LB@@LB@Position - mRNA 134 -- 160@LB@Position - sRNA 3 -- 29@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 3 -- 9@LB@
152	0.006159	0.1406	b2467	nudK	-9.74	0.053801	255 -- 261	1 -- 7	GDP-mannose pyrophosphatase	_____________254_____262\n_____________\|_______\|\n5'-CAA...AUUUC_______ACAAC...AAG-3'\n______________ACCCUGC\n______________\|\|\|\|\|:\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	255 -- 261	1 -- 7	-11.9691 kcal/mol	0.21022 kcal/mol	2.03949 kcal/mol	254 262@LB@ \| \|@LB@5'-CAA...AUUUC ACAAC...AAG-3'@LB@ ACCCUGC@LB@ \|\|\|\|\|:\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -11.9691 kcal/mol@LB@Unfolding Energy - mRNA 0.21022 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 255 -- 261@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 1 -- 7@LB@
30	0.0002612	0.03063	b3789	rffH	-9.15	0.078548	244 -- 254	48 -- 58	glucose-1-phosphate thymidylyltransferase	_____________243_________255\n_____________\|___________\|\n5'-GGC...AUUGC___________CGCGG...UGA-3'\n______________AUCCGAUUACG\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________UAGGCUAAUGC\n3'-AUU...CGGUU___________ACUUC...AUG-5'\n_____________\|___________\|\n_____________59__________47\n	248 -- 254	48 -- 54	-15.7126 kcal/mol	4.98099 kcal/mol	1.55987 kcal/mol	243 255@LB@ \| \|@LB@5'-GGC...AUUGC CGCGG...UGA-3'@LB@ AUCCGAUUACG@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ UAGGCUAAUGC@LB@3'-AUU...CGGUU ACUUC...AUG-5'@LB@ \| \|@LB@ 59 47@LB@@LB@@LB@Energy -9.15 kcal/mol@LB@Hybridization Energy -15.7126 kcal/mol@LB@Unfolding Energy - mRNA 4.98099 kcal/mol@LB@Unfolding Energy - sRNA 1.55987 kcal/mol@LB@@LB@Position - mRNA 244 -- 254@LB@Position - sRNA 48 -- 58@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 48 -- 54@LB@
15	5.569e-05	0.01183	b1761	gdhA	-12.26	0.008568	280 -- 294	40 -- 56	glutamate dehydrogenase NADP-specific	_____________279_______________295\n_____________\|_________________\|\n5'-UAA...GCAAG___UU____________UGACCA-3'\n______________CCG____CGUGAAGUAA\n______________\|\|\|____\|\|\|\|\|\|\|\|\|\|\n______________GGC____GCACUUCAUU\n3'-AUU...GUUUA___UAAU__________UUCCA...AUG-5'\n_____________\|_________________\|\n_____________57________________39\n	288 -- 294	40 -- 46	-17.1153 kcal/mol	1.5283 kcal/mol	3.34823 kcal/mol	279 295@LB@ \| \|@LB@5'-UAA...GCAAG UU UGACCA-3'@LB@ CCG CGUGAAGUAA@LB@ \|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ GGC GCACUUCAUU@LB@3'-AUU...GUUUA UAAU UUCCA...AUG-5'@LB@ \| \|@LB@ 57 39@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -17.1153 kcal/mol@LB@Unfolding Energy - mRNA 1.5283 kcal/mol@LB@Unfolding Energy - sRNA 3.34823 kcal/mol@LB@@LB@Position - mRNA 280 -- 294@LB@Position - sRNA 40 -- 56@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 40 -- 46@LB@
48	0.0005438	0.03933	b0811	glnH	-6.95	0.268265	70 -- 77	1 -- 8	glutamine transporter subunit	_____________69_______78\n_____________\|________\|\n5'-AUC...GGUAU________AAAUC...CCG-3'\n______________UGCCCUAU\n______________\|:\|\|\|\|\|:\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	71 -- 77	1 -- 7	-10.5963 kcal/mol	1.549 kcal/mol	2.11603 kcal/mol	69 78@LB@ \| \|@LB@5'-AUC...GGUAU AAAUC...CCG-3'@LB@ UGCCCUAU@LB@ \|:\|\|\|\|\|:@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -6.95 kcal/mol@LB@Hybridization Energy -10.5963 kcal/mol@LB@Unfolding Energy - mRNA 1.549 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 70 -- 77@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 71 -- 77@LB@Position Seed - sRNA 1 -- 7@LB@
62	0.001059	0.05856	b3927	glpF	-11.40	0.016677	192 -- 203	48 -- 59	glycerol facilitator	_____________191__________204\n_____________\|____________\|\n5'-GAU...UUCAG____________AGUCA...AAG-3'\n______________GAUCCGAUUAUG\n______________:\|\|\|\|\|\|\|\|\|:\|\n______________UUAGGCUAAUGC\n3'-AUU...UCGGU____________ACUUC...AUG-5'\n_____________\|____________\|\n_____________60___________47\n	197 -- 203	48 -- 54	-15.6898 kcal/mol	2.7066 kcal/mol	1.55995 kcal/mol	191 204@LB@ \| \|@LB@5'-GAU...UUCAG AGUCA...AAG-3'@LB@ GAUCCGAUUAUG@LB@ :\|\|\|\|\|\|\|\|\|:\|@LB@ UUAGGCUAAUGC@LB@3'-AUU...UCGGU ACUUC...AUG-5'@LB@ \| \|@LB@ 60 47@LB@@LB@@LB@Energy -11.40 kcal/mol@LB@Hybridization Energy -15.6898 kcal/mol@LB@Unfolding Energy - mRNA 2.7066 kcal/mol@LB@Unfolding Energy - sRNA 1.55995 kcal/mol@LB@@LB@Position - mRNA 192 -- 203@LB@Position - sRNA 48 -- 59@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 48 -- 54@LB@
180	0.007734	0.1511	b3617	kbl	-7.53	0.200168	193 -- 210	43 -- 60	glycine C-acetyltransferase	_____________192________________211\n_____________\|__________________\|\n5'-UUU...CUGGA_____GCAA_________AAUUU...AAG-3'\n______________GAAUC____UGCGUGGAG\n______________:\|\|\|\|____\|:\|\|\|\|:\|\|\n______________UUUAG____AUGCACUUC\n3'-AUU...GUCGG_____GCUA_________AUUUU...AUG-5'\n_____________\|__________________\|\n_____________61_________________42\n	204 -- 210	43 -- 49	-15.3085 kcal/mol	4.51554 kcal/mol	3.27548 kcal/mol	192 211@LB@ \| \|@LB@5'-UUU...CUGGA GCAA AAUUU...AAG-3'@LB@ GAAUC UGCGUGGAG@LB@ :\|\|\|\| \|:\|\|\|\|:\|\|@LB@ UUUAG AUGCACUUC@LB@3'-AUU...GUCGG GCUA AUUUU...AUG-5'@LB@ \| \|@LB@ 61 42@LB@@LB@@LB@Energy -7.53 kcal/mol@LB@Hybridization Energy -15.3085 kcal/mol@LB@Unfolding Energy - mRNA 4.51554 kcal/mol@LB@Unfolding Energy - sRNA 3.27548 kcal/mol@LB@@LB@Position - mRNA 193 -- 210@LB@Position - sRNA 43 -- 60@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 43 -- 49@LB@
165	0.006672	0.1423	b1651	gloA	-7.94	0.160716	101 -- 108	49 -- 56	glyoxalase I Ni-dependent	_____________100______109\n_____________\|________\|\n5'-UGG...CUAUA________CCGAC...AAA-3'\n______________CCGAUUAC\n______________\|\|\|\|\|\|\|\|\n______________GGCUAAUG\n3'-AUU...GUUUA________CACUU...AUG-5'\n_____________\|________\|\n_____________57_______48\n	102 -- 108	49 -- 55	-10.6899 kcal/mol	2.44421 kcal/mol	0.317884 kcal/mol	100 109@LB@ \| \|@LB@5'-UGG...CUAUA CCGAC...AAA-3'@LB@ CCGAUUAC@LB@ \|\|\|\|\|\|\|\|@LB@ GGCUAAUG@LB@3'-AUU...GUUUA CACUU...AUG-5'@LB@ \| \|@LB@ 57 48@LB@@LB@@LB@Energy -7.94 kcal/mol@LB@Hybridization Energy -10.6899 kcal/mol@LB@Unfolding Energy - mRNA 2.44421 kcal/mol@LB@Unfolding Energy - sRNA 0.317884 kcal/mol@LB@@LB@Position - mRNA 101 -- 108@LB@Position - sRNA 49 -- 56@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 49 -- 55@LB@
141	0.00519	0.1284	b3550	yiaC	-7.29	0.227336	55 -- 76	1 -- 22	GNAT family putative N-acetyltransferase	_____________54______________________77\n_____________\|_______________________\|\n5'-AAU...CAUCU_____C______CGAC_______CUAAG...GGC-3'\n______________ACGUC__GCCUC____GCCCUAU\n______________\|\|:\|\|__:\|\|\|\|____:\|\|\|\|\|:\n______________UGUAG__UGGAG____UGGGAUG\n3'-AUU...UAUCU_____AA_____ACA________-5'\n_____________\|______________________\|\n_____________23_____________________1\n	70 -- 76	1 -- 7	-20.6815 kcal/mol	5.4259 kcal/mol	7.99236 kcal/mol	54 77@LB@ \| \|@LB@5'-AAU...CAUCU C CGAC CUAAG...GGC-3'@LB@ ACGUC GCCUC GCCCUAU@LB@ \|\|:\|\| :\|\|\|\| :\|\|\|\|\|:@LB@ UGUAG UGGAG UGGGAUG@LB@3'-AUU...UAUCU AA ACA -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -7.29 kcal/mol@LB@Hybridization Energy -20.6815 kcal/mol@LB@Unfolding Energy - mRNA 5.4259 kcal/mol@LB@Unfolding Energy - sRNA 7.99236 kcal/mol@LB@@LB@Position - mRNA 55 -- 76@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 70 -- 76@LB@Position Seed - sRNA 1 -- 7@LB@
107	0.003396	0.1101	b3871	typA	-9.79	0.052081	283 -- 294	47 -- 59	GTP-binding protein	_____________282___________295\n_____________\|_____________\|\n5'-UGC...CCAAC_____________UCGACU-3'\n______________AAUCCGGU_ACGU\n______________\|\|\|\|\|\|:\|_\|\|\|\|\n______________UUAGGCUA_UGCA\n3'-AUU...UCGGU________A____CUUCA...AUG-5'\n_____________\|_____________\|\n_____________60____________46\n	284 -- 290	52 -- 58	-13.0383 kcal/mol	1.68034 kcal/mol	1.56852 kcal/mol	282 295@LB@ \| \|@LB@5'-UGC...CCAAC UCGACU-3'@LB@ AAUCCGGU ACGU@LB@ \|\|\|\|\|\|:\| \|\|\|\|@LB@ UUAGGCUA UGCA@LB@3'-AUU...UCGGU A CUUCA...AUG-5'@LB@ \| \|@LB@ 60 46@LB@@LB@@LB@Energy -9.79 kcal/mol@LB@Hybridization Energy -13.0383 kcal/mol@LB@Unfolding Energy - mRNA 1.68034 kcal/mol@LB@Unfolding Energy - sRNA 1.56852 kcal/mol@LB@@LB@Position - mRNA 283 -- 294@LB@Position - sRNA 47 -- 59@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 52 -- 58@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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