Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
54	0.0007615	0.04961	b0313	betI	-7.44	0.209816	47 -- 68	32 -- 57	choline-inducible betIBA-betT divergent operon transcriptional repressor	_____________46_________________________69\n_____________\|__________________________\|\n5'-CAG...CCUUU___C________U_____________UCAUC...CGC-3'\n______________UCC___UGC_UG__GUGAAAGGUCUG\n______________\|\|\|___\|:\|_\|\|__\|\|:\|\|\|\|\|\|\|\|\|\n______________AGG___AUG_AC__CAUUUUCCAGAC\n3'-AUU...GGUUU___CUA___C__UU____________UUUCU...AUG-5'\n_____________\|__________________________\|\n_____________58_________________________31\n	62 -- 68	32 -- 38	-22.1015 kcal/mol	6.64828 kcal/mol	7.92091 kcal/mol	46 69@LB@ \| \|@LB@5'-CAG...CCUUU C U UCAUC...CGC-3'@LB@ UCC UGC UG GUGAAAGGUCUG@LB@ \|\|\| \|:\| \|\| \|\|:\|\|\|\|\|\|\|\|\|@LB@ AGG AUG AC CAUUUUCCAGAC@LB@3'-AUU...GGUUU CUA C UU UUUCU...AUG-5'@LB@ \| \|@LB@ 58 31@LB@@LB@@LB@Energy -7.44 kcal/mol@LB@Hybridization Energy -22.1015 kcal/mol@LB@Unfolding Energy - mRNA 6.64828 kcal/mol@LB@Unfolding Energy - sRNA 7.92091 kcal/mol@LB@@LB@Position - mRNA 47 -- 68@LB@Position - sRNA 32 -- 57@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 32 -- 38@LB@
7	1.5e-05	0.00754	b0720	gltA	-8.97	0.087950	70 -- 79	4 -- 13	citrate synthase	_____________69_________80\n_____________\|__________\|\n5'-CGG...CAGAG__________AGGUU...GUA-3'\n______________CUCUGUACCC\n______________\|\|\|\|\|\|\|\|\|\|\n______________GAGACAUGGG\n3'-AUU...GAAUG__________AUG-5'\n_____________\|__________\|\n_____________14_________3\n	73 -- 79	4 -- 10	-18.8657 kcal/mol	4.79377 kcal/mol	5.12835 kcal/mol	69 80@LB@ \| \|@LB@5'-CGG...CAGAG AGGUU...GUA-3'@LB@ CUCUGUACCC@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ GAGACAUGGG@LB@3'-AUU...GAAUG AUG-5'@LB@ \| \|@LB@ 14 3@LB@@LB@@LB@Energy -8.97 kcal/mol@LB@Hybridization Energy -18.8657 kcal/mol@LB@Unfolding Energy - mRNA 4.79377 kcal/mol@LB@Unfolding Energy - sRNA 5.12835 kcal/mol@LB@@LB@Position - mRNA 70 -- 79@LB@Position - sRNA 4 -- 13@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 4 -- 10@LB@
128	0.004347	0.1195	b3690	cbrA	-9.56	0.060736	197 -- 211	17 -- 31	colicin M resistance protein; FAD-binding protein putative oxidoreductase	_____________196_____________212\n_____________\|_______________\|\n5'-CAG...AGUGA_______________CGACG...AAA-3'\n______________AAAGAUGGAACAUUU\n______________\|\|\|\|\|\|:\|\|\|\|\|\|:\|\n______________UUUCUAUCUUGUAGA\n3'-AUU...CAGAC_______________AUGGA...AUG-5'\n_____________\|_______________\|\n_____________32______________16\n	204 -- 210	18 -- 24	-17.0326 kcal/mol	3.22818 kcal/mol	4.26586 kcal/mol	196 212@LB@ \| \|@LB@5'-CAG...AGUGA CGACG...AAA-3'@LB@ AAAGAUGGAACAUUU@LB@ \|\|\|\|\|\|:\|\|\|\|\|\|:\|@LB@ UUUCUAUCUUGUAGA@LB@3'-AUU...CAGAC AUGGA...AUG-5'@LB@ \| \|@LB@ 32 16@LB@@LB@@LB@Energy -9.56 kcal/mol@LB@Hybridization Energy -17.0326 kcal/mol@LB@Unfolding Energy - mRNA 3.22818 kcal/mol@LB@Unfolding Energy - sRNA 4.26586 kcal/mol@LB@@LB@Position - mRNA 197 -- 211@LB@Position - sRNA 17 -- 31@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 18 -- 24@LB@
61	0.0009386	0.05413	b0574	cusB	-12.39	0.007718	27 -- 84	1 -- 59	copper/silver efflux system membrane fusion protein	_____________26_____________________________________________________________85\n_____________\|______________________________________________________________\|\n5'-AAA...AUCAC______________CC_____CU__C_G____U__CCAU___C______A_________C__AGACG...AAA-3'\n______________GAUCCGAUUGC_UG__GUGAA__GG_C_GAGA_GA____GCG_UUUACC_UC___ACCC_GC\n______________:\|\|\|\|\|\|\|\|:\|_\|\|__\|\|:\|\|__\|\|_\|_\|\|:\|_\|\|____:\|:_:\|\|\|\|\|_\|\|___\|\|\|\|_:\|\n______________UUAGGCUAAUG_AC__CAUUU__CC_G_CUUU_CU____UGU_GAAUGG_AG___UGGG_UG\n3'-AUU...UCGGU___________C__UU_____U___A_A_______AUCU___A_________ACA____A__-5'\n_____________\|_____________________________________________________________\|\n_____________60____________________________________________________________1\n	31 -- 37	49 -- 55	-39.7476 kcal/mol	14.6812 kcal/mol	12.7027 kcal/mol	26 85@LB@ \| \|@LB@5'-AAA...AUCAC CC CU C G U CCAU C A C AGACG...AAA-3'@LB@ GAUCCGAUUGC UG GUGAA GG C GAGA GA GCG UUUACC UC ACCC GC@LB@ :\|\|\|\|\|\|\|\|:\| \|\| \|\|:\|\| \|\| \| \|\|:\| \|\| :\|: :\|\|\|\|\| \|\| \|\|\|\| :\|@LB@ UUAGGCUAAUG AC CAUUU CC G CUUU CU UGU GAAUGG AG UGGG UG@LB@3'-AUU...UCGGU C UU U A A AUCU A ACA A -5'@LB@ \| \|@LB@ 60 1@LB@@LB@@LB@Energy -12.39 kcal/mol@LB@Hybridization Energy -39.7476 kcal/mol@LB@Unfolding Energy - mRNA 14.6812 kcal/mol@LB@Unfolding Energy - sRNA 12.7027 kcal/mol@LB@@LB@Position - mRNA 27 -- 84@LB@Position - sRNA 1 -- 59@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 49 -- 55@LB@
78	0.001629	0.07345	b2624	alpA	-3.42	0.861549	231 -- 237	49 -- 55	CP4-57 prophage; DNA-binding transcriptional activator	_____________230_____238\n_____________\|_______\|\n5'-CGC...UACUA_______CAGCG...AAU-3'\n______________CGGUUAC\n______________\|\|:\|\|\|\|\n______________GCUAAUG\n3'-AUU...UUUAG_______CACUU...AUG-5'\n_____________\|_______\|\n_____________56______48\n	231 -- 237	49 -- 55	-7.04991 kcal/mol	3.33366 kcal/mol	0.311712 kcal/mol	230 238@LB@ \| \|@LB@5'-CGC...UACUA CAGCG...AAU-3'@LB@ CGGUUAC@LB@ \|\|:\|\|\|\|@LB@ GCUAAUG@LB@3'-AUU...UUUAG CACUU...AUG-5'@LB@ \| \|@LB@ 56 48@LB@@LB@@LB@Energy -3.42 kcal/mol@LB@Hybridization Energy -7.04991 kcal/mol@LB@Unfolding Energy - mRNA 3.33366 kcal/mol@LB@Unfolding Energy - sRNA 0.311712 kcal/mol@LB@@LB@Position - mRNA 231 -- 237@LB@Position - sRNA 49 -- 55@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 49 -- 55@LB@
136	0.004892	0.1265	b2349	intS	-8.19	0.139381	273 -- 281	3 -- 11	CPS-53 (KpLE1) prophage; putative prophage CPS-53 integrase	_____________272_______282\n_____________\|_________\|\n5'-AAG...AUGGC_________UAUGU...GGA-3'\n______________CUGUACCUU\n______________\|\|\|\|\|\|\|:\|\n______________GACAUGGGA\n3'-AUU...AUGGA_________UG-5'\n_____________\|_________\|\n_____________12________2\n	275 -- 281	3 -- 9	-13.0022 kcal/mol	1.37836 kcal/mol	3.45478 kcal/mol	272 282@LB@ \| \|@LB@5'-AAG...AUGGC UAUGU...GGA-3'@LB@ CUGUACCUU@LB@ \|\|\|\|\|\|\|:\|@LB@ GACAUGGGA@LB@3'-AUU...AUGGA UG-5'@LB@ \| \|@LB@ 12 2@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -13.0022 kcal/mol@LB@Unfolding Energy - mRNA 1.37836 kcal/mol@LB@Unfolding Energy - sRNA 3.45478 kcal/mol@LB@@LB@Position - mRNA 273 -- 281@LB@Position - sRNA 3 -- 11@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 3 -- 9@LB@
199	0.008811	0.1558	b4515	cydX	-11.13	0.020449	151 -- 184	5 -- 31	cytochrome d (bd-I) ubiquinol oxidase subunit X	_____________150________________________________185\n_____________\|__________________________________\|\n5'-ACC...CCAAA_______UU______AAC___CACUC________AAGUA...GUC-3'\n______________GAAGAUA__GAACGU___ACC_____UCUGUACU\n______________:\|\|\|\|\|\|__\|\|\|\|:\|___\|\|\|_____\|\|\|\|\|\|\|:\n______________UUUCUAU__CUUGUA___UGG_____AGACAUGG\n3'-AUU...CAGAC_______________GAA________________GAUG-5'\n_____________\|__________________________________\|\n_____________32_________________________________4\n	178 -- 184	5 -- 11	-22.8813 kcal/mol	3.72257 kcal/mol	8.05162 kcal/mol	150 185@LB@ \| \|@LB@5'-ACC...CCAAA UU AAC CACUC AAGUA...GUC-3'@LB@ GAAGAUA GAACGU ACC UCUGUACU@LB@ :\|\|\|\|\|\| \|\|\|\|:\| \|\|\| \|\|\|\|\|\|\|:@LB@ UUUCUAU CUUGUA UGG AGACAUGG@LB@3'-AUU...CAGAC GAA GAUG-5'@LB@ \| \|@LB@ 32 4@LB@@LB@@LB@Energy -11.13 kcal/mol@LB@Hybridization Energy -22.8813 kcal/mol@LB@Unfolding Energy - mRNA 3.72257 kcal/mol@LB@Unfolding Energy - sRNA 8.05162 kcal/mol@LB@@LB@Position - mRNA 151 -- 184@LB@Position - sRNA 5 -- 31@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 5 -- 11@LB@
24	0.0002009	0.02687	b1487	ddpA	-9.43	0.065911	261 -- 269	48 -- 56	D D-dipeptide ABC transporter periplasmic binding protein	_____________260_______270\n_____________\|_________\|\n5'-UCA...AUUUC_________CACGC...UGA-3'\n______________CCGGUUGCG\n______________\|\|\|:\|\|:\|\|\n______________GGCUAAUGC\n3'-AUU...GUUUA_________ACUUC...AUG-5'\n_____________\|_________\|\n_____________57________47\n	263 -- 269	48 -- 54	-13.2683 kcal/mol	2.26031 kcal/mol	1.55919 kcal/mol	260 270@LB@ \| \|@LB@5'-UCA...AUUUC CACGC...UGA-3'@LB@ CCGGUUGCG@LB@ \|\|\|:\|\|:\|\|@LB@ GGCUAAUGC@LB@3'-AUU...GUUUA ACUUC...AUG-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -9.43 kcal/mol@LB@Hybridization Energy -13.2683 kcal/mol@LB@Unfolding Energy - mRNA 2.26031 kcal/mol@LB@Unfolding Energy - sRNA 1.55919 kcal/mol@LB@@LB@Position - mRNA 261 -- 269@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 48 -- 54@LB@
137	0.004974	0.127	b0092	ddlB	-6.99	0.263821	283 -- 297	43 -- 56	D-alanine:D-alanine ligase	_____________282_____________298\n_____________\|_______________\|\n5'-UUC...GUUAG____AC_________GCG-3'\n______________CCGG__UGCGUGAAG\n______________\|\|\|:__\|:\|\|\|\|\|\|\|\n______________GGCU__AUGCACUUC\n3'-AUU...GUUUA____A__________AUUUU...AUG-5'\n_____________\|_______________\|\n_____________57______________42\n	291 -- 297	43 -- 49	-17.3937 kcal/mol	7.14584 kcal/mol	3.27327 kcal/mol	282 298@LB@ \| \|@LB@5'-UUC...GUUAG AC GCG-3'@LB@ CCGG UGCGUGAAG@LB@ \|\|\|: \|:\|\|\|\|\|\|\|@LB@ GGCU AUGCACUUC@LB@3'-AUU...GUUUA A AUUUU...AUG-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -6.99 kcal/mol@LB@Hybridization Energy -17.3937 kcal/mol@LB@Unfolding Energy - mRNA 7.14584 kcal/mol@LB@Unfolding Energy - sRNA 3.27327 kcal/mol@LB@@LB@Position - mRNA 283 -- 297@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 291 -- 297@LB@Position Seed - sRNA 43 -- 49@LB@
22	0.0001652	0.02641	b3566	xylF	-9.94	0.047349	202 -- 240	1 -- 33	D-xylose transporter subunit	_____________201_____________________________________241\n_____________\|_______________________________________\|\n5'-CGA...CACCA_____AUAA________C__CUCAC______C____C__UCCUG...AUG-3'\n______________UGAAA____AGAACAUU_UA_____CCUUUG_ACCU_AC\n______________\|\|\|\|\|____\|\|\|\|\|\|\|:_\|\|_____\|\|\|:\|\|_\|\|\|:_\|\|\n______________ACUUU____UCUUGUAG_AU_____GGAGAC_UGGG_UG\n3'-AUU...UCCAG_____CUA_________A_____________A____A__-5'\n_____________\|______________________________________\|\n_____________34_____________________________________1\n	212 -- 218	18 -- 24	-22.447 kcal/mol	3.48977 kcal/mol	9.04529 kcal/mol	201 241@LB@ \| \|@LB@5'-CGA...CACCA AUAA C CUCAC C C UCCUG...AUG-3'@LB@ UGAAA AGAACAUU UA CCUUUG ACCU AC@LB@ \|\|\|\|\| \|\|\|\|\|\|\|: \|\| \|\|\|:\|\| \|\|\|: \|\|@LB@ ACUUU UCUUGUAG AU GGAGAC UGGG UG@LB@3'-AUU...UCCAG CUA A A A -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -22.447 kcal/mol@LB@Unfolding Energy - mRNA 3.48977 kcal/mol@LB@Unfolding Energy - sRNA 9.04529 kcal/mol@LB@@LB@Position - mRNA 202 -- 240@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 18 -- 24@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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