Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
1	0	0	b0757	galK	-15.06	0.000658	183 -- 231	8 -- 60	galactokinase		_____________182_____________________________________________________232\n_____________\|_______________________________________________________\|\n5'-CAU...UUCGC_______GA______U___AAAU_____________AAA____CA__________UUGCC...GUG-3'\n______________GAAUCCG_____GUG_AAG____GAG_UCUGAAAGA___AACA____AUCUCUGU\n______________:\|\|\|\|\|\|_____\|\|\|_\|\|\|____:\|\|_\|\|\|\|\|\|\|\|\|___\|\|\|\|____\|:\|\|\|\|\|\|\n______________UUUAGGC_____CAC_UUC____UUC_AGACUUUCU___UUGU____UGGAGACA\n3'-AUU...GUCGG_______UAAUG_______AUU____C_________AUC____AGAA________UGGGAUG-5'\n_____________\|_______________________________________________________\|\n_____________61______________________________________________________7\n	225 -- 231	8 -- 14	-35.0906 kcal/mol	7.3474 kcal/mol	12.6967 kcal/mol	182 232@LB@ \| \|@LB@5'-CAU...UUCGC GA U AAAU AAA CA UUGCC...GUG-3'@LB@ GAAUCCG GUG AAG GAG UCUGAAAGA AACA AUCUCUGU@LB@ :\|\|\|\|\|\| \|\|\| \|\|\| :\|\| \|\|\|\|\|\|\|\|\| \|\|\|\| \|:\|\|\|\|\|\|@LB@ UUUAGGC CAC UUC UUC AGACUUUCU UUGU UGGAGACA@LB@3'-AUU...GUCGG UAAUG AUU C AUC AGAA UGGGAUG-5'@LB@ \| \|@LB@ 61 7@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -35.0906 kcal/mol@LB@Unfolding Energy - mRNA 7.3474 kcal/mol@LB@Unfolding Energy - sRNA 12.6967 kcal/mol@LB@@LB@Position - mRNA 183 -- 231@LB@Position - sRNA 8 -- 60@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 8 -- 14@LB@
2	0	0	b0796	ybiH	-7.52	0.201543	119 -- 140	54 -- 76	DUF1956 domain-containing tetR family putative transcriptional regulator	b1013	_____________118_____________________141\n_____________\|_______________________\|\n5'-CGG...GCGUU________UC_______UA____GACAA...CCA-3'\n______________GCUAAAAU__UUAGCCA___CUG\n______________\|\|\|\|\|\|\|\|__\|:\|\|\|\|\|___\|:\|\n______________CGAUUUUA__AGUCGGU___GGC\n3'-AUU...CCCGC________UA_______UUA___UAAUG...AUG-5'\n_____________\|_______________________\|\n_____________77______________________53\n	129 -- 135	60 -- 66	-19.7488 kcal/mol	5.88928 kcal/mol	6.3634 kcal/mol	118 141@LB@ \| \|@LB@5'-CGG...GCGUU UC UA GACAA...CCA-3'@LB@ GCUAAAAU UUAGCCA CUG@LB@ \|\|\|\|\|\|\|\| \|:\|\|\|\|\| \|:\|@LB@ CGAUUUUA AGUCGGU GGC@LB@3'-AUU...CCCGC UA UUA UAAUG...AUG-5'@LB@ \| \|@LB@ 77 53@LB@@LB@@LB@Energy -7.52 kcal/mol@LB@Hybridization Energy -19.7488 kcal/mol@LB@Unfolding Energy - mRNA 5.88928 kcal/mol@LB@Unfolding Energy - sRNA 6.3634 kcal/mol@LB@@LB@Position - mRNA 119 -- 140@LB@Position - sRNA 54 -- 76@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 60 -- 66@LB@
3	0	0	b2564	pdxJ	-5.03	0.588876	273 -- 283	52 -- 62	pyridoxine 5'-phosphate synthase		_____________272_________284\n_____________\|___________\|\n5'-AAA...CUUAC___________GCAGG...UUG-3'\n______________CCGGAUCCGGU\n______________\|\|::\|\|\|\|\|:\|\n______________GGUUUAGGCUA\n3'-AUU...AAGUC___________AUGCA...AUG-5'\n_____________\|___________\|\n_____________63__________51\n	277 -- 283	52 -- 58	-14.6263 kcal/mol	4.30712 kcal/mol	5.31395 kcal/mol	272 284@LB@ \| \|@LB@5'-AAA...CUUAC GCAGG...UUG-3'@LB@ CCGGAUCCGGU@LB@ \|\|::\|\|\|\|\|:\|@LB@ GGUUUAGGCUA@LB@3'-AUU...AAGUC AUGCA...AUG-5'@LB@ \| \|@LB@ 63 51@LB@@LB@@LB@Energy -5.03 kcal/mol@LB@Hybridization Energy -14.6263 kcal/mol@LB@Unfolding Energy - mRNA 4.30712 kcal/mol@LB@Unfolding Energy - sRNA 5.31395 kcal/mol@LB@@LB@Position - mRNA 273 -- 283@LB@Position - sRNA 52 -- 62@LB@Position Seed - mRNA 277 -- 283@LB@Position Seed - sRNA 52 -- 58@LB@
4	8.02e-06	0.007054	b0425	panE	-14.83	0.000836	22 -- 49	1 -- 32	2-dehydropantoate reductase NADPH-specific		_____________21________________________________50\n_____________\|_________________________________\|\n5'-AUC...AUAUC_________________UU____________U_AUUUU...CGC-3'\n______________GAAAGAUGG__CAUCUU__CCUCU___CCCU_C\n______________\|\|\|\|\|\|\|:\|__\|\|\|\|\|\|__\|\|\|\|\|___\|\|\|\|_\|\n______________CUUUCUAUC__GUAGAA__GGAGA___GGGA_G\n3'-AUU...CCAGA_________UU______U______CAU____U_-5'\n_____________\|________________________________\|\n_____________33_______________________________1\n	24 -- 30	24 -- 30	-28.6113 kcal/mol	4.98037 kcal/mol	8.82471 kcal/mol	21 50@LB@ \| \|@LB@5'-AUC...AUAUC UU U AUUUU...CGC-3'@LB@ GAAAGAUGG CAUCUU CCUCU CCCU C@LB@ \|\|\|\|\|\|\|:\| \|\|\|\|\|\| \|\|\|\|\| \|\|\|\| \|@LB@ CUUUCUAUC GUAGAA GGAGA GGGA G@LB@3'-AUU...CCAGA UU U CAU U -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -14.83 kcal/mol@LB@Hybridization Energy -28.6113 kcal/mol@LB@Unfolding Energy - mRNA 4.98037 kcal/mol@LB@Unfolding Energy - sRNA 8.82471 kcal/mol@LB@@LB@Position - mRNA 22 -- 49@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 24 -- 30@LB@Position Seed - sRNA 24 -- 30@LB@
5	1.055e-05	0.007422	b1287	yciW	-9.98	0.045809	266 -- 289	4 -- 27	putative oxidoreductase		_____________265______________________290\n_____________\|________________________\|\n5'-GUC...ACGGA___U______C________GU___GGAAG...CAA-3'\n______________GUA_GACGUU_UGCCUCUG__CCC\n______________:\|\|_:\|\|:\|:_\|:\|\|\|\|\|\|__\|\|\|\n______________UAU_UUGUAG_AUGGAGAC__GGG\n3'-AUU...CUUUC___C______A________AU___AUG-5'\n_____________\|________________________\|\n_____________28_______________________3\n	278 -- 284	9 -- 15	-26.8114 kcal/mol	8.80708 kcal/mol	8.0435 kcal/mol	265 290@LB@ \| \|@LB@5'-GUC...ACGGA U C GU GGAAG...CAA-3'@LB@ GUA GACGUU UGCCUCUG CCC@LB@ :\|\| :\|\|:\|: \|:\|\|\|\|\|\| \|\|\|@LB@ UAU UUGUAG AUGGAGAC GGG@LB@3'-AUU...CUUUC C A AU AUG-5'@LB@ \| \|@LB@ 28 3@LB@@LB@@LB@Energy -9.98 kcal/mol@LB@Hybridization Energy -26.8114 kcal/mol@LB@Unfolding Energy - mRNA 8.80708 kcal/mol@LB@Unfolding Energy - sRNA 8.0435 kcal/mol@LB@@LB@Position - mRNA 266 -- 289@LB@Position - sRNA 4 -- 27@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 9 -- 15@LB@
6	1.448e-05	0.00754	b3863	polA	-11.40	0.016745	236 -- 262	1 -- 29	5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease		_____________235___________________________263\n_____________\|_____________________________\|\n5'-AGA...CUUGU______UU_________________GC__AUAUC...GCG-3'\n______________AGAUGG__CAUCUUAUCUU__UAUC__GC\n______________\|\|\|\|:\|__\|\|\|\|\|\|\|:\|\|:__\|\|:\|__:\|\n______________UCUAUC__GUAGAAUGGAG__AUGG__UG\n3'-AUU...GACUU______UU___________AC____GA__-5'\n_____________\|____________________________\|\n_____________30___________________________1\n	244 -- 250	15 -- 21	-25.0693 kcal/mol	5.64295 kcal/mol	8.05079 kcal/mol	235 263@LB@ \| \|@LB@5'-AGA...CUUGU UU GC AUAUC...GCG-3'@LB@ AGAUGG CAUCUUAUCUU UAUC GC@LB@ \|\|\|\|:\| \|\|\|\|\|\|\|:\|\|: \|\|:\| :\|@LB@ UCUAUC GUAGAAUGGAG AUGG UG@LB@3'-AUU...GACUU UU AC GA -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -11.40 kcal/mol@LB@Hybridization Energy -25.0693 kcal/mol@LB@Unfolding Energy - mRNA 5.64295 kcal/mol@LB@Unfolding Energy - sRNA 8.05079 kcal/mol@LB@@LB@Position - mRNA 236 -- 262@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 15 -- 21@LB@
7	1.5e-05	0.00754	b0720	gltA	-8.97	0.087950	70 -- 79	4 -- 13	citrate synthase		_____________69_________80\n_____________\|__________\|\n5'-CGG...CAGAG__________AGGUU...GUA-3'\n______________CUCUGUACCC\n______________\|\|\|\|\|\|\|\|\|\|\n______________GAGACAUGGG\n3'-AUU...GAAUG__________AUG-5'\n_____________\|__________\|\n_____________14_________3\n	73 -- 79	4 -- 10	-18.8657 kcal/mol	4.79377 kcal/mol	5.12835 kcal/mol	69 80@LB@ \| \|@LB@5'-CGG...CAGAG AGGUU...GUA-3'@LB@ CUCUGUACCC@LB@ \|\|\|\|\|\|\|\|\|\|@LB@ GAGACAUGGG@LB@3'-AUU...GAAUG AUG-5'@LB@ \| \|@LB@ 14 3@LB@@LB@@LB@Energy -8.97 kcal/mol@LB@Hybridization Energy -18.8657 kcal/mol@LB@Unfolding Energy - mRNA 4.79377 kcal/mol@LB@Unfolding Energy - sRNA 5.12835 kcal/mol@LB@@LB@Position - mRNA 70 -- 79@LB@Position - sRNA 4 -- 13@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 4 -- 10@LB@
8	1.842e-05	0.007771	b2797	sdaB	-15.07	0.000654	133 -- 147	55 -- 69	L-serine dehydratase 2		_____________132_____________148\n_____________\|_______________\|\n5'-CGA...CUCUC_______________UUUCG...UGA-3'\n______________UGUUCAGCUAAGUCC\n______________\|:\|\|\|\|\|\|:\|\|:\|\|\|\n______________AUAAGUCGGUUUAGG\n3'-AUU...AUUUU_______________CUAAU...AUG-5'\n_____________\|_______________\|\n_____________70______________54\n	141 -- 147	55 -- 61	-22.3059 kcal/mol	1.18067 kcal/mol	6.0797 kcal/mol	132 148@LB@ \| \|@LB@5'-CGA...CUCUC UUUCG...UGA-3'@LB@ UGUUCAGCUAAGUCC@LB@ \|:\|\|\|\|\|\|:\|\|:\|\|\|@LB@ AUAAGUCGGUUUAGG@LB@3'-AUU...AUUUU CUAAU...AUG-5'@LB@ \| \|@LB@ 70 54@LB@@LB@@LB@Energy -15.07 kcal/mol@LB@Hybridization Energy -22.3059 kcal/mol@LB@Unfolding Energy - mRNA 1.18067 kcal/mol@LB@Unfolding Energy - sRNA 6.0797 kcal/mol@LB@@LB@Position - mRNA 133 -- 147@LB@Position - sRNA 55 -- 69@LB@Position Seed - mRNA 141 -- 147@LB@Position Seed - sRNA 55 -- 61@LB@
9	1.988e-05	0.007771	b1985	yeeO	-11.51	0.015356	224 -- 255	1 -- 39	putative multdrug exporter MATE family		_____________223_____________________________________256\n_____________\|_______________________________________\|\n5'-GAU...CAAUU______GGCUUU___________________________GCCAG...GCU-3'\n______________AAGGUC_________GAAUAUCU__CCUCUG__CUUUAC\n______________\|\|\|\|\|\|_________\|\|\|:\|\|\|\|__\|\|\|\|\|\|__\|::\|\|\|\n______________UUCCAG_________CUUGUAGA__GGAGAC__GGGAUG\n3'-AUU...UCAUU______ACUUUCUAU________AU______AU______-5'\n_____________\|______________________________________\|\n_____________40_____________________________________1\n	237 -- 243	17 -- 23	-28.0657 kcal/mol	5.21805 kcal/mol	11.3576 kcal/mol	223 256@LB@ \| \|@LB@5'-GAU...CAAUU GGCUUU GCCAG...GCU-3'@LB@ AAGGUC GAAUAUCU CCUCUG CUUUAC@LB@ \|\|\|\|\|\| \|\|\|:\|\|\|\| \|\|\|\|\|\| \|::\|\|\|@LB@ UUCCAG CUUGUAGA GGAGAC GGGAUG@LB@3'-AUU...UCAUU ACUUUCUAU AU AU -5'@LB@ \| \|@LB@ 40 1@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -28.0657 kcal/mol@LB@Unfolding Energy - mRNA 5.21805 kcal/mol@LB@Unfolding Energy - sRNA 11.3576 kcal/mol@LB@@LB@Position - mRNA 224 -- 255@LB@Position - sRNA 1 -- 39@LB@Position Seed - mRNA 237 -- 243@LB@Position Seed - sRNA 17 -- 23@LB@
10	2.605e-05	0.009165	b3599	mtlA	-16.58	0.000118	1 -- 31	4 -- 28	mannitol-specific PTS enzyme: IIA IIB and IIC components		______________1_______________________________32\n______________\|_______________________________\|\n___________5'-___________ACGUCAA________C_____CCUAU...UCA-3'\n______________GAU_GAACGUC_______UUACCUCU_UACCC\n______________\|\|\|_\|\|\|\|:\|\|_______\|\|\|\|\|\|\|\|_\|\|\|\|\|\n______________CUA_CUUGUAG_______AAUGGAGA_AUGGG\n3'-AUU...ACUUU___U______________________C_____AUG-5'\n_____________\|________________________________\|\n_____________29_______________________________3\n	19 -- 25	10 -- 16	-27.7982 kcal/mol	3.19385 kcal/mol	8.04825 kcal/mol	1 32@LB@ \| \|@LB@ 5'- ACGUCAA C CCUAU...UCA-3'@LB@ GAU GAACGUC UUACCUCU UACCC@LB@ \|\|\| \|\|\|\|:\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|@LB@ CUA CUUGUAG AAUGGAGA AUGGG@LB@3'-AUU...ACUUU U C AUG-5'@LB@ \| \|@LB@ 29 3@LB@@LB@@LB@Energy -16.58 kcal/mol@LB@Hybridization Energy -27.7982 kcal/mol@LB@Unfolding Energy - mRNA 3.19385 kcal/mol@LB@Unfolding Energy - sRNA 8.04825 kcal/mol@LB@@LB@Position - mRNA 1 -- 31@LB@Position - sRNA 4 -- 28@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 10 -- 16@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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