Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
20	9.41e-05	0.01655	b0721	sdhC	-12.56	0.006704	146 -- 157	4 -- 15	succinate dehydrogenase membrane subunit binds cytochrome b556	_____________145__________158\n_____________\|____________\|\n5'-UCC...AGUUA____________GUAGU...CCG-3'\n______________ACCUCUGUGCCC\n______________\|\|\|\|\|\|\|\|:\|\|\|\n______________UGGAGACAUGGG\n3'-AUU...UAGAA____________AUG-5'\n_____________\|____________\|\n_____________16___________3\n	151 -- 157	4 -- 10	-23.3451 kcal/mol	4.99288 kcal/mol	5.82084 kcal/mol	145 158@LB@ \| \|@LB@5'-UCC...AGUUA GUAGU...CCG-3'@LB@ ACCUCUGUGCCC@LB@ \|\|\|\|\|\|\|\|:\|\|\|@LB@ UGGAGACAUGGG@LB@3'-AUU...UAGAA AUG-5'@LB@ \| \|@LB@ 16 3@LB@@LB@@LB@Energy -12.56 kcal/mol@LB@Hybridization Energy -23.3451 kcal/mol@LB@Unfolding Energy - mRNA 4.99288 kcal/mol@LB@Unfolding Energy - sRNA 5.82084 kcal/mol@LB@@LB@Position - mRNA 146 -- 157@LB@Position - sRNA 4 -- 15@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 4 -- 10@LB@
12	3.867e-05	0.01134	b0728	sucC	-9.87	0.049323	145 -- 186	43 -- 73	succinyl-CoA synthetase beta subunit	_____________144_________________________________________187\n_____________\|___________________________________________\|\n5'-CGC...GAUAC_____________UACGGUUUAAA____AA_____________GAUGG...AAG-3'\n______________GAAAUAUUCGGUC___________AGAU__CGAUUAC_UGAAG\n______________:\|\|\|\|\|\|\|\|:\|:\|___________\|:\|\|__\|\|\|\|\|\|\|_\|\|\|\|\|\n______________UUUUAUAAGUCGG___________UUUA__GCUAAUG_ACUUC\n3'-AUU...GCCGA____________________________G________C_____AUUUU...AUG-5'\n_____________\|___________________________________________\|\n_____________74__________________________________________42\n	175 -- 181	49 -- 55	-26.5157 kcal/mol	7.48621 kcal/mol	9.17828 kcal/mol	144 187@LB@ \| \|@LB@5'-CGC...GAUAC UACGGUUUAAA AA GAUGG...AAG-3'@LB@ GAAAUAUUCGGUC AGAU CGAUUAC UGAAG@LB@ :\|\|\|\|\|\|\|\|:\|:\| \|:\|\| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUUUAUAAGUCGG UUUA GCUAAUG ACUUC@LB@3'-AUU...GCCGA G C AUUUU...AUG-5'@LB@ \| \|@LB@ 74 42@LB@@LB@@LB@Energy -9.87 kcal/mol@LB@Hybridization Energy -26.5157 kcal/mol@LB@Unfolding Energy - mRNA 7.48621 kcal/mol@LB@Unfolding Energy - sRNA 9.17828 kcal/mol@LB@@LB@Position - mRNA 145 -- 186@LB@Position - sRNA 43 -- 73@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 49 -- 55@LB@
75	0.001328	0.06227	b1748	astC	-8.39	0.124654	121 -- 127	1 -- 7	succinylornithine transaminase PLP-dependent	_____________120_____128\n_____________\|_______\|\n5'-UCA...CACGA_______AAUCU...UGU-3'\n______________ACCCUGC\n______________\|\|\|\|\|:\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	121 -- 127	1 -- 7	-11.9071 kcal/mol	1.50434 kcal/mol	2.03949 kcal/mol	120 128@LB@ \| \|@LB@5'-UCA...CACGA AAUCU...UGU-3'@LB@ ACCCUGC@LB@ \|\|\|\|\|:\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -8.39 kcal/mol@LB@Hybridization Energy -11.9071 kcal/mol@LB@Unfolding Energy - mRNA 1.50434 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 121 -- 127@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 121 -- 127@LB@Position Seed - sRNA 1 -- 7@LB@
114	0.003723	0.1147	b2327	yfcA	-8.38	0.125082	22 -- 29	1 -- 8	TauE/TSUP family inner membrane protein	_____________21_______30\n_____________\|________\|\n5'-CAU...UACGU________CGAUA...GUG-3'\n______________UGCCCUGC\n______________\|:\|\|\|\|:\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	23 -- 29	1 -- 7	-12.241 kcal/mol	1.76759 kcal/mol	2.11603 kcal/mol	21 30@LB@ \| \|@LB@5'-CAU...UACGU CGAUA...GUG-3'@LB@ UGCCCUGC@LB@ \|:\|\|\|\|:\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -8.38 kcal/mol@LB@Hybridization Energy -12.241 kcal/mol@LB@Unfolding Energy - mRNA 1.76759 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 22 -- 29@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 1 -- 7@LB@
140	0.005114	0.1284	b3993	thiE	-7.64	0.188958	243 -- 250	4 -- 11	thiamine phosphate synthase (thiamine phosphate pyrophosphorylase)	_____________242______251\n_____________\|________\|\n5'-CAC...CAGGA________GGUGG...UAC-3'\n______________CUGUACCC\n______________\|\|\|\|\|\|\|\|\n______________GACAUGGG\n3'-AUU...AUGGA________AUG-5'\n_____________\|________\|\n_____________12_______3\n	244 -- 250	4 -- 10	-13.9053 kcal/mol	2.83489 kcal/mol	3.45361 kcal/mol	242 251@LB@ \| \|@LB@5'-CAC...CAGGA GGUGG...UAC-3'@LB@ CUGUACCC@LB@ \|\|\|\|\|\|\|\|@LB@ GACAUGGG@LB@3'-AUU...AUGGA AUG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -7.64 kcal/mol@LB@Hybridization Energy -13.9053 kcal/mol@LB@Unfolding Energy - mRNA 2.83489 kcal/mol@LB@Unfolding Energy - sRNA 3.45361 kcal/mol@LB@@LB@Position - mRNA 243 -- 250@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 4 -- 10@LB@
89	0.002377	0.09395	b4136	dsbD	-10.47	0.032769	211 -- 231	1 -- 22	thiol:disulfide interchange protein and activator of DsbC	_____________210____________________232\n_____________\|______________________\|\n5'-UGC...UCAAC_______UA_G___________UACUU...CGG-3'\n______________GCAUCUU__C_CUG_AUCCUGC\n______________:\|\|\|\|\|\|__\|_\|\|\|_\|:\|\|\|:\|\n______________UGUAGAA__G_GAC_UGGGAUG\n3'-AUU...UAUCU_______UG_A___A_______-5'\n_____________\|_____________________\|\n_____________23____________________1\n	225 -- 231	1 -- 7	-23.5098 kcal/mol	5.0672 kcal/mol	7.99236 kcal/mol	210 232@LB@ \| \|@LB@5'-UGC...UCAAC UA G UACUU...CGG-3'@LB@ GCAUCUU C CUG AUCCUGC@LB@ :\|\|\|\|\|\| \| \|\|\| \|:\|\|\|:\|@LB@ UGUAGAA G GAC UGGGAUG@LB@3'-AUU...UAUCU UG A A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -10.47 kcal/mol@LB@Hybridization Energy -23.5098 kcal/mol@LB@Unfolding Energy - mRNA 5.0672 kcal/mol@LB@Unfolding Energy - sRNA 7.99236 kcal/mol@LB@@LB@Position - mRNA 211 -- 231@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 1 -- 7@LB@
120	0.003999	0.1172	b2582	trxC	-9.28	0.072721	206 -- 219	4 -- 16	thioredoxin 2	_____________205____________220\n_____________\|______________\|\n5'-CUC...AUGAA____G_________AUUGU...UUG-3'\n______________UACC_UUUGUACCC\n______________\|\|\|\|_\|:\|\|\|\|\|\|\|\n______________AUGG_AGACAUGGG\n3'-AUU...GUAGA______________AUG-5'\n_____________\|______________\|\n_____________17_____________3\n	213 -- 219	4 -- 10	-18.1109 kcal/mol	2.27225 kcal/mol	6.59015 kcal/mol	205 220@LB@ \| \|@LB@5'-CUC...AUGAA G AUUGU...UUG-3'@LB@ UACC UUUGUACCC@LB@ \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ AUGG AGACAUGGG@LB@3'-AUU...GUAGA AUG-5'@LB@ \| \|@LB@ 17 3@LB@@LB@@LB@Energy -9.28 kcal/mol@LB@Hybridization Energy -18.1109 kcal/mol@LB@Unfolding Energy - mRNA 2.27225 kcal/mol@LB@Unfolding Energy - sRNA 6.59015 kcal/mol@LB@@LB@Position - mRNA 206 -- 219@LB@Position - sRNA 4 -- 16@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 4 -- 10@LB@
65	0.001093	0.05856	b1519	tam	-11.83	0.011992	187 -- 231	1 -- 50	trans-aconitate methyltransferase	_____________186____________________________________________________232\n_____________\|______________________________________________________\|\n5'-UUU...CGCUG_____________AAAU______C_________C__________U___A_____ACUUU...AAU-3'\n______________ACG_GGAGUAAAA____GUCUGA____UGGAAC______CCUCU_UAU_UCUAC\n______________\|\|\|_\|:\|\|\|\|\|\|\|____\|\|\|\|\|\|____\|:\|\|\|\|______\|\|\|\|\|_\|\|:_:\|\|\|\|\n______________UGC_CUUCAUUUU____CAGACU____AUCUUG______GGAGA_AUG_GGAUG\n3'-AUU...GCUAA___A_________C_________UUCU______UAGAAU_____C_________-5'\n_____________\|_____________________________________________________\|\n_____________51____________________________________________________1\n	191 -- 197	39 -- 45	-32.7669 kcal/mol	8.27841 kcal/mol	12.6772 kcal/mol	186 232@LB@ \| \|@LB@5'-UUU...CGCUG AAAU C C U A ACUUU...AAU-3'@LB@ ACG GGAGUAAAA GUCUGA UGGAAC CCUCU UAU UCUAC@LB@ \|\|\| \|:\|\|\|\|\|\|\| \|\|\|\|\|\| \|:\|\|\|\| \|\|\|\|\| \|\|: :\|\|\|\|@LB@ UGC CUUCAUUUU CAGACU AUCUUG GGAGA AUG GGAUG@LB@3'-AUU...GCUAA A C UUCU UAGAAU C -5'@LB@ \| \|@LB@ 51 1@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -32.7669 kcal/mol@LB@Unfolding Energy - mRNA 8.27841 kcal/mol@LB@Unfolding Energy - sRNA 12.6772 kcal/mol@LB@@LB@Position - mRNA 187 -- 231@LB@Position - sRNA 1 -- 50@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 39 -- 45@LB@
188	0.008316	0.1556	b3406	greB	-8.11	0.146040	42 -- 55	52 -- 65	transcript cleavage factor	_____________41_____________56\n_____________\|______________\|\n5'-AUU...GUCAC______________AUGAA...UGA-3'\n______________CAGCUAAAUCUGGU\n______________\|\|\|\|:\|\|\|\|\|:\|:\|\n______________GUCGGUUUAGGCUA\n3'-AUU...UAUAA______________AUGCA...AUG-5'\n_____________\|______________\|\n_____________66_____________51\n	49 -- 55	52 -- 58	-18.5321 kcal/mol	4.27254 kcal/mol	6.17277 kcal/mol	41 56@LB@ \| \|@LB@5'-AUU...GUCAC AUGAA...UGA-3'@LB@ CAGCUAAAUCUGGU@LB@ \|\|\|\|:\|\|\|\|\|:\|:\|@LB@ GUCGGUUUAGGCUA@LB@3'-AUU...UAUAA AUGCA...AUG-5'@LB@ \| \|@LB@ 66 51@LB@@LB@@LB@Energy -8.11 kcal/mol@LB@Hybridization Energy -18.5321 kcal/mol@LB@Unfolding Energy - mRNA 4.27254 kcal/mol@LB@Unfolding Energy - sRNA 6.17277 kcal/mol@LB@@LB@Position - mRNA 42 -- 55@LB@Position - sRNA 52 -- 65@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 52 -- 58@LB@
91	0.002527	0.0977	b4393	trpR	-12.48	0.007130	9 -- 21	43 -- 55	transcriptional repressor tryptophan-binding	_____________8_____________22\n_____________\|_____________\|\n___5'-GAGACGCG_____________AACGU...AUC-3'\n______________CGGUUAUGUGAAG\n______________\|\|:\|\|\|:\|\|\|\|\|\|\n______________GCUAAUGCACUUC\n3'-AUU...UUUAG_____________AUUUU...AUG-5'\n_____________\|_____________\|\n_____________56____________42\n	15 -- 21	43 -- 49	-16.824 kcal/mol	1.08541 kcal/mol	3.2693 kcal/mol	8 22@LB@ \| \|@LB@ 5'-GAGACGCG AACGU...AUC-3'@LB@ CGGUUAUGUGAAG@LB@ \|\|:\|\|\|:\|\|\|\|\|\|@LB@ GCUAAUGCACUUC@LB@3'-AUU...UUUAG AUUUU...AUG-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -12.48 kcal/mol@LB@Hybridization Energy -16.824 kcal/mol@LB@Unfolding Energy - mRNA 1.08541 kcal/mol@LB@Unfolding Energy - sRNA 3.2693 kcal/mol@LB@@LB@Position - mRNA 9 -- 21@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 43 -- 49@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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