Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
199	0.02363	0.3833	b4141	yjeH	-8.04	0.585705	57 -- 67	64 -- 74	putative transporter	_____________56_____________68\n_____________\|______________\|\n5'-CAG...AAAUC___G_______C__AAAUU...CUG-3'\n______________UAC_CAGUUGC_CA\n______________\|\|\|_\|\|\|\|::\|_\|\|\n______________AUG_GUCAGUG_GU\n3'-UUU...UGGUU___A_______U__ACUAC...UAG-5'\n_____________\|______________\|\n_____________75_____________63\n	163 -- 169	170 -- 176	-11.2481 kcal/mol	2.09942 kcal/mol	0.433965 kcal/mol	56 68@LB@ \| \|@LB@5'-CAG...AAAUC G C AAAUU...CUG-3'@LB@ UAC CAGUUGC CA@LB@ \|\|\| \|\|\|\|::\| \|\|@LB@ AUG GUCAGUG GU@LB@3'-UUU...UGGUU A U ACUAC...UAG-5'@LB@ \| \|@LB@ 75 63@LB@@LB@@LB@Energy -8.04 kcal/mol@LB@Hybridization Energy -11.2481 kcal/mol@LB@Unfolding Energy - mRNA 2.09942 kcal/mol@LB@Unfolding Energy - sRNA 0.433965 kcal/mol@LB@@LB@Position - mRNA 57 -- 67@LB@Position - sRNA 64 -- 74@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 170 -- 176@LB@
138	0.01487	0.3575	b3771	ilvD	-15.83	0.018324	35 -- 66	67 -- 98	dihydroxyacid dehydratase	_____________34______67\n_____________\|_______\|\n5'-GCG...AAAAA_______GCACG...GUA-3'\n______________UGGGACG\n______________\|\|\|\|\|\|\|\n______________ACCCUGC\n3'-UUU...GCAAC_______GAAUU...UAG-5'\n_____________\|_______\|\n_____________99______66\n	147 -- 153	87 -- 93	-12.7493 kcal/mol	2.26202 kcal/mol	4.21173 kcal/mol	34 67@LB@ \| \|@LB@5'-GCG...AAAAA GCACG...GUA-3'@LB@ UGGGACG@LB@ \|\|\|\|\|\|\|@LB@ ACCCUGC@LB@3'-UUU...GCAAC GAAUU...UAG-5'@LB@ \| \|@LB@ 99 66@LB@@LB@@LB@Energy -15.83 kcal/mol@LB@Hybridization Energy -12.7493 kcal/mol@LB@Unfolding Energy - mRNA 2.26202 kcal/mol@LB@Unfolding Energy - sRNA 4.21173 kcal/mol@LB@@LB@Position - mRNA 35 -- 66@LB@Position - sRNA 67 -- 98@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 87 -- 93@LB@
46	0.004081	0.2985	b2466	ypfG	-10.37	0.233995	256 -- 280	68 -- 95	DUF1176 family protein	_____________255_____281\n_____________\|_______\|\n5'-AAA...CAAAC_______UUAAU...UCA-3'\n______________GUCACAU\n______________\|\|\|\|\|\|:\n______________CAGUGUG\n3'-UUU...UGAGU_______UACUA...UAG-5'\n_____________\|_______\|\n_____________96______67\n	57 -- 63	168 -- 174	-9.42181 kcal/mol	2.13246 kcal/mol	0.260649 kcal/mol	255 281@LB@ \| \|@LB@5'-AAA...CAAAC UUAAU...UCA-3'@LB@ GUCACAU@LB@ \|\|\|\|\|\|:@LB@ CAGUGUG@LB@3'-UUU...UGAGU UACUA...UAG-5'@LB@ \| \|@LB@ 96 67@LB@@LB@@LB@Energy -10.37 kcal/mol@LB@Hybridization Energy -9.42181 kcal/mol@LB@Unfolding Energy - mRNA 2.13246 kcal/mol@LB@Unfolding Energy - sRNA 0.260649 kcal/mol@LB@@LB@Position - mRNA 256 -- 280@LB@Position - sRNA 68 -- 95@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 168 -- 174@LB@
152	0.0171	0.3749	b3605	lldD	-10.23	0.248595	212 -- 221	68 -- 77	L-lactate dehydrogenase FMN-linked	_____________211_______222\n_____________\|_________\|\n5'-UUU...GAAAA_________UAUUU...ACA-3'\n______________ACGCAUGAU\n______________\|\|:\|\|\|\|\|\|\n______________UGUGUACUA\n3'-UUU...GUCAG_________CGUCC...UAG-5'\n_____________\|_________\|\n_____________78________67\n	199 -- 205	163 -- 169	-11.1438 kcal/mol	1.00002 kcal/mol	4.14962 kcal/mol	211 222@LB@ \| \|@LB@5'-UUU...GAAAA UAUUU...ACA-3'@LB@ ACGCAUGAU@LB@ \|\|:\|\|\|\|\|\|@LB@ UGUGUACUA@LB@3'-UUU...GUCAG CGUCC...UAG-5'@LB@ \| \|@LB@ 78 67@LB@@LB@@LB@Energy -10.23 kcal/mol@LB@Hybridization Energy -11.1438 kcal/mol@LB@Unfolding Energy - mRNA 1.00002 kcal/mol@LB@Unfolding Energy - sRNA 4.14962 kcal/mol@LB@@LB@Position - mRNA 212 -- 221@LB@Position - sRNA 68 -- 77@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 163 -- 169@LB@
43	0.003721	0.2912	b3232	zapE	-10.85	0.189153	236 -- 256	70 -- 90	divisome ATPase	_____________235___________257\n_____________\|_____________\|\n5'-AGG...AACGG____UU_______AACCG...AAA-3'\n______________CCUU__UUUCGUC\n______________\|\|\|\|__:\|\|\|:\|\|\n______________GGAA__GAAGUAG\n3'-UUU...GUGGG____C________-5'\n_____________\|____________\|\n_____________91___________70\n	146 -- 152	1 -- 7	-9.27362 kcal/mol	0.691484 kcal/mol	1.04836 kcal/mol	235 257@LB@ \| \|@LB@5'-AGG...AACGG UU AACCG...AAA-3'@LB@ CCUU UUUCGUC@LB@ \|\|\|\| :\|\|\|:\|\|@LB@ GGAA GAAGUAG@LB@3'-UUU...GUGGG C -5'@LB@ \| \|@LB@ 91 70@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -9.27362 kcal/mol@LB@Unfolding Energy - mRNA 0.691484 kcal/mol@LB@Unfolding Energy - sRNA 1.04836 kcal/mol@LB@@LB@Position - mRNA 236 -- 256@LB@Position - sRNA 70 -- 90@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 1 -- 7@LB@
101	0.01097	0.3575	b1882	cheY	-8.64	0.475467	226 -- 255	72 -- 99	chemotaxis regulator transmitting signal to flagellar motor component	_____________225_______________256\n_____________\|_________________\|\n5'-GCG...AGGCG_______GGA_______GCAAA...UUG-3'\n______________GGGGCAU___CCCUUGC\n______________\|\|\|\|\|\|:___\|\|\|\|\|\|\|\n______________CCCCGUG___GGGAACG\n3'-UUU...GCGUA_______G_________AAGUAG-5'\n_____________\|_________________\|\n_____________100_______________71\n	38 -- 44	7 -- 13	-25.3164 kcal/mol	8.48869 kcal/mol	10.1447 kcal/mol	225 256@LB@ \| \|@LB@5'-GCG...AGGCG GGA GCAAA...UUG-3'@LB@ GGGGCAU CCCUUGC@LB@ \|\|\|\|\|\|: \|\|\|\|\|\|\|@LB@ CCCCGUG GGGAACG@LB@3'-UUU...GCGUA G AAGUAG-5'@LB@ \| \|@LB@ 100 71@LB@@LB@@LB@Energy -8.64 kcal/mol@LB@Hybridization Energy -25.3164 kcal/mol@LB@Unfolding Energy - mRNA 8.48869 kcal/mol@LB@Unfolding Energy - sRNA 10.1447 kcal/mol@LB@@LB@Position - mRNA 226 -- 255@LB@Position - sRNA 72 -- 99@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 7 -- 13@LB@
147	0.01564	0.358	b3153	yhbO	-15.63	0.020103	154 -- 186	72 -- 98	stress-resistance protein	_____________153_______________________________187\n_____________\|_________________________________\|\n5'-GUU...AUCCC__A________AAUU___CUCAU__________AACGC...UUA-3'\n______________CG_UUGUGGGA____CGC_____CCAAUGCAAC\n______________\|\|_\|\|\|\|\|\|\|\|____\|\|\|_____\|\|\|\|:\|\|\|\|\|\n______________GC_AACACCCU____GCG_____GGUUGCGUUG\n3'-UUU...AAUUA__________________AAUU___________GUCGU...UAG-5'\n_____________\|_________________________________\|\n_____________99________________________________71\n	180 -- 186	72 -- 78	-31.4938 kcal/mol	8.17407 kcal/mol	13.9341 kcal/mol	153 187@LB@ \| \|@LB@5'-GUU...AUCCC A AAUU CUCAU AACGC...UUA-3'@LB@ CG UUGUGGGA CGC CCAAUGCAAC@LB@ \|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|:\|\|\|\|\|@LB@ GC AACACCCU GCG GGUUGCGUUG@LB@3'-UUU...AAUUA AAUU GUCGU...UAG-5'@LB@ \| \|@LB@ 99 71@LB@@LB@@LB@Energy -15.63 kcal/mol@LB@Hybridization Energy -31.4938 kcal/mol@LB@Unfolding Energy - mRNA 8.17407 kcal/mol@LB@Unfolding Energy - sRNA 13.9341 kcal/mol@LB@@LB@Position - mRNA 154 -- 186@LB@Position - sRNA 72 -- 98@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 72 -- 78@LB@
155	0.01733	0.3749	b3542	dppC	-14.36	0.036537	40 -- 62	74 -- 93	dipeptide/heme ABC transporter permease	_____________39_______________________________________________________63\n_____________\|________________________________________________________\|\n5'-GGG...UCGUC____U__U___C__________UA___C_____GA_______C_____________AGAAG...UCG-3'\n______________AACU_GC_GGU__GAUCUGCUG__CGG_GUGGU___AC_CCG_GUAUUC_GUCAUA\n______________\|\|\|:_\|\|_\|\|\|__:\|\|\|\|\|\|\|\|__\|\|\|_\|\|\|:\|___\|\|_\|\|:_:\|\|:\|\|_\|\|\|\|:\|\n______________UUGG_CG_CCA__UUAGACGAC__GCC_CACUA___UG_GGU_UAUGAG_CAGUGU\n_____3'-UUUUUU____U__U___UA_________C__________AAA__U__________U______GUACU...UAG-5'\n_____________\|________________________________________________________\|\n_____________94_______________________________________________________73\n	146 -- 152	200 -- 206	-46.3163 kcal/mol	15.5571 kcal/mol	18.3074 kcal/mol	39 63@LB@ \| \|@LB@5'-GGG...UCGUC U U C UA C GA C AGAAG...UCG-3'@LB@ AACU GC GGU GAUCUGCUG CGG GUGGU AC CCG GUAUUC GUCAUA@LB@ \|\|\|: \|\| \|\|\| :\|\|\|\|\|\|\|\| \|\|\| \|\|\|:\| \|\| \|\|: :\|\|:\|\| \|\|\|\|:\|@LB@ UUGG CG CCA UUAGACGAC GCC CACUA UG GGU UAUGAG CAGUGU@LB@ 3'-UUUUUU U U UA C AAA U U GUACU...UAG-5'@LB@ \| \|@LB@ 94 73@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -46.3163 kcal/mol@LB@Unfolding Energy - mRNA 15.5571 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 40 -- 62@LB@Position - sRNA 74 -- 93@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 200 -- 206@LB@
193	0.0225	0.3833	b3751	rbsB	-14.76	0.030308	38 -- 56	74 -- 93	D-ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor	_____________37________________________________________________57\n_____________\|_________________________________________________\|\n___5'-GCGGUACG_____GG______GAAAA_____CA___U____________________UUAAU...UCU-3'\n______________AGUCU__CGGGCG_____GGUCG__UUG_UGGGACGUU_GAUCGGCGCA\n______________\|\|\|\|:__\|::\|\|:_____::\|\|\|__\|\|\|_\|\|\|\|\|\|\|:\|_:\|:\|::\|\|\|\|\n______________UCAGG__GUUCGU_____UUAGC__AAC_ACCCUGCGA_UUGGUUGCGU\n3'-UUU...CGUAG_____AA______ACCAA____________________A__________UGGUC...UAG-5'\n_____________\|_________________________________________________\|\n_____________94________________________________________________73\n	40 -- 46	85 -- 91	-47.7546 kcal/mol	17.619 kcal/mol	19.1533 kcal/mol	37 57@LB@ \| \|@LB@ 5'-GCGGUACG GG GAAAA CA U UUAAU...UCU-3'@LB@ AGUCU CGGGCG GGUCG UUG UGGGACGUU GAUCGGCGCA@LB@ \|\|\|\|: \|::\|\|: ::\|\|\| \|\|\| \|\|\|\|\|\|\|:\| :\|:\|::\|\|\|\|@LB@ UCAGG GUUCGU UUAGC AAC ACCCUGCGA UUGGUUGCGU@LB@3'-UUU...CGUAG AA ACCAA A UGGUC...UAG-5'@LB@ \| \|@LB@ 94 73@LB@@LB@@LB@Energy -14.76 kcal/mol@LB@Hybridization Energy -47.7546 kcal/mol@LB@Unfolding Energy - mRNA 17.619 kcal/mol@LB@Unfolding Energy - sRNA 19.1533 kcal/mol@LB@@LB@Position - mRNA 38 -- 56@LB@Position - sRNA 74 -- 93@LB@Position Seed - mRNA 40 -- 46@LB@Position Seed - sRNA 85 -- 91@LB@
136	0.01456	0.3575	b0369	hemB	-9.31	0.367566	203 -- 221	75 -- 90	5-aminolevulinate dehydratase (porphobilinogen synthase)	_____________202________________222\n_____________\|__________________\|\n5'-CCA...UAAAA_______ACCC_______GGUAU...UGC-3'\n______________CAUCCCU____UGCUUCA\n______________\|\|:\|\|\|:____\|\|\|\|\|\|\|\n______________GUGGGGG____ACGAAGU\n3'-UUU...ACCCC_______A__________AG-5'\n_____________\|__________________\|\n_____________91_________________74\n	153 -- 159	3 -- 9	-17.8942 kcal/mol	1.92924 kcal/mol	7.09151 kcal/mol	202 222@LB@ \| \|@LB@5'-CCA...UAAAA ACCC GGUAU...UGC-3'@LB@ CAUCCCU UGCUUCA@LB@ \|\|:\|\|\|: \|\|\|\|\|\|\|@LB@ GUGGGGG ACGAAGU@LB@3'-UUU...ACCCC A AG-5'@LB@ \| \|@LB@ 91 74@LB@@LB@@LB@Energy -9.31 kcal/mol@LB@Hybridization Energy -17.8942 kcal/mol@LB@Unfolding Energy - mRNA 1.92924 kcal/mol@LB@Unfolding Energy - sRNA 7.09151 kcal/mol@LB@@LB@Position - mRNA 203 -- 221@LB@Position - sRNA 75 -- 90@LB@Position Seed - mRNA 153 -- 159@LB@Position Seed - sRNA 3 -- 9@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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