Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
115	0.01273	0.3575	b3440	yhhX	-9.67	0.316530	203 -- 215	123 -- 136	putative oxidoreductase	_____________202______________________________216\n_____________\|________________________________\|\n5'-GAC...CGUAU_______AC____AG____A__U____U____CGCAUA-3'\n______________GUACUUA__CGCA___GAU_GC_GGCA_GUCG\n______________:\|\|\|\|:\|__\|:\|\|___\|\|\|_\|\|_\|\|\|\|_\|\|\|:\n______________UAUGAGU__GUGU___CUA_CG_CCGU_CAGU\n3'-UUU...GUGGU_______CA____GUA______U____U____UGAAA...UAG-5'\n_____________\|________________________________\|\n_____________137______________________________122\n	264 -- 270	175 -- 181	-25.3028 kcal/mol	7.53441 kcal/mol	8.15082 kcal/mol	202 216@LB@ \| \|@LB@5'-GAC...CGUAU AC AG A U U CGCAUA-3'@LB@ GUACUUA CGCA GAU GC GGCA GUCG@LB@ :\|\|\|\|:\| \|:\|\| \|\|\| \|\| \|\|\|\| \|\|\|:@LB@ UAUGAGU GUGU CUA CG CCGU CAGU@LB@3'-UUU...GUGGU CA GUA U U UGAAA...UAG-5'@LB@ \| \|@LB@ 137 122@LB@@LB@@LB@Energy -9.67 kcal/mol@LB@Hybridization Energy -25.3028 kcal/mol@LB@Unfolding Energy - mRNA 7.53441 kcal/mol@LB@Unfolding Energy - sRNA 8.15082 kcal/mol@LB@@LB@Position - mRNA 203 -- 215@LB@Position - sRNA 123 -- 136@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 175 -- 181@LB@
121	0.01323	0.3575	b3429	glgA	-14.56	0.033318	190 -- 240	123 -- 176	glycogen synthase	_____________189__________________241\n_____________\|____________________\|\n5'-GUA...AUGCA__________GU________AGAUG...ACG-3'\n______________GGUUUUACAU__AUGUUCAG\n______________\|:\|\|\|\|\|\|\|:__\|\|::\|\|\|\|\n______________CUAAAAUGUG__UAUGAGUC\n3'-UUU...GCCCA__________GU________AGUGU...UAG-5'\n_____________\|____________________\|\n_____________177__________________122\n	219 -- 225	174 -- 180	-15.9439 kcal/mol	3.46677 kcal/mol	4.94566 kcal/mol	189 241@LB@ \| \|@LB@5'-GUA...AUGCA GU AGAUG...ACG-3'@LB@ GGUUUUACAU AUGUUCAG@LB@ \|:\|\|\|\|\|\|\|: \|\|::\|\|\|\|@LB@ CUAAAAUGUG UAUGAGUC@LB@3'-UUU...GCCCA GU AGUGU...UAG-5'@LB@ \| \|@LB@ 177 122@LB@@LB@@LB@Energy -14.56 kcal/mol@LB@Hybridization Energy -15.9439 kcal/mol@LB@Unfolding Energy - mRNA 3.46677 kcal/mol@LB@Unfolding Energy - sRNA 4.94566 kcal/mol@LB@@LB@Position - mRNA 190 -- 240@LB@Position - sRNA 123 -- 176@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 174 -- 180@LB@
146	0.01559	0.358	b4394	yjjX	-8.60	0.483218	101 -- 114	123 -- 137	non-canonical purine NTP phosphatase ITPase/XTPase	_____________100__________________________________115\n_____________\|____________________________________\|\n5'-UGC...AAUCC______A______________UC________UU___CGAGA...CAU-3'\n______________CGCUAA_AUUCAG__GC_CAU__UGCAGGCA__UCA\n______________\|:\|:\|\|_\|:\|\|\|\|__:\|_\|\|\|__\|\|\|\|\|\|\|\|__\|\|\|\n______________GUGGUU_UGAGUC__UG_GUA__ACGUCCGU__AGU\n3'-UUU...AAAAU______A______AG__U___CU________UC___UGAAA...UAG-5'\n_____________\|____________________________________\|\n_____________138__________________________________122\n	254 -- 260	156 -- 162	-32.2061 kcal/mol	8.98762 kcal/mol	12.5635 kcal/mol	100 115@LB@ \| \|@LB@5'-UGC...AAUCC A UC UU CGAGA...CAU-3'@LB@ CGCUAA AUUCAG GC CAU UGCAGGCA UCA@LB@ \|:\|:\|\| \|:\|\|\|\| :\| \|\|\| \|\|\|\|\|\|\|\| \|\|\|@LB@ GUGGUU UGAGUC UG GUA ACGUCCGU AGU@LB@3'-UUU...AAAAU A AG U CU UC UGAAA...UAG-5'@LB@ \| \|@LB@ 138 122@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -32.2061 kcal/mol@LB@Unfolding Energy - mRNA 8.98762 kcal/mol@LB@Unfolding Energy - sRNA 12.5635 kcal/mol@LB@@LB@Position - mRNA 101 -- 114@LB@Position - sRNA 123 -- 137@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 156 -- 162@LB@
150	0.01679	0.3749	b3550	yiaC	-14.78	0.030008	125 -- 180	123 -- 174	GNAT family putative N-acetyltransferase	_____________124_______________________________________________181\n_____________\|_________________________________________________\|\n5'-AAU...UCUGU_____CU_______G_C________U___G___GA______________GGCUG...GGC-3'\n______________UACUC__UUAUGCA_G_AUGUGGGC_GGU_AAU__UCA____UGUGGUU\n______________\|\|\|\|\|__\|:\|::\|\|_\|_\|\|\|::\|\|\|_:\|\|_\|\|:__\|\|\|____\|\|:\|\|\|:\n______________AUGAG__AGUGUGU_C_UACGUCCG_UCA_UUG__AGU____ACGCCAG\n3'-UUU...UGGUU_____UC_______A__________U___G___AA___CUUA_______UAGGG...UAG-5'\n_____________\|_________________________________________________\|\n_____________175_______________________________________________122\n	134 -- 140	167 -- 173	-39.4647 kcal/mol	14.8163 kcal/mol	12.8033 kcal/mol	124 181@LB@ \| \|@LB@5'-AAU...UCUGU CU G C U G GA GGCUG...GGC-3'@LB@ UACUC UUAUGCA G AUGUGGGC GGU AAU UCA UGUGGUU@LB@ \|\|\|\|\| \|:\|::\|\| \| \|\|\|::\|\|\| :\|\| \|\|: \|\|\| \|\|:\|\|\|:@LB@ AUGAG AGUGUGU C UACGUCCG UCA UUG AGU ACGCCAG@LB@3'-UUU...UGGUU UC A U G AA CUUA UAGGG...UAG-5'@LB@ \| \|@LB@ 175 122@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -39.4647 kcal/mol@LB@Unfolding Energy - mRNA 14.8163 kcal/mol@LB@Unfolding Energy - sRNA 12.8033 kcal/mol@LB@@LB@Position - mRNA 125 -- 180@LB@Position - sRNA 123 -- 174@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 167 -- 173@LB@
186	0.02152	0.3833	b3581	sgbH	-10.17	0.255482	135 -- 158	123 -- 153	3-keto-L-gulonate 6-phosphate decarboxylase	_____________134________159\n_____________\|__________\|\n5'-UUU...UAAGG__________UAUGA...AAG-3'\n______________AG_CACACAU\n______________\|\|_\|\|\|\|\|\|\|\n______________UC_GUGUGUA\n3'-UUU...AUGAG__A_______CUACG...UAG-5'\n_____________\|__________\|\n_____________154________122\n	193 -- 199	166 -- 172	-9.15702 kcal/mol	1.98532 kcal/mol	0.591348 kcal/mol	134 159@LB@ \| \|@LB@5'-UUU...UAAGG UAUGA...AAG-3'@LB@ AG CACACAU@LB@ \|\| \|\|\|\|\|\|\|@LB@ UC GUGUGUA@LB@3'-UUU...AUGAG A CUACG...UAG-5'@LB@ \| \|@LB@ 154 122@LB@@LB@@LB@Energy -10.17 kcal/mol@LB@Hybridization Energy -9.15702 kcal/mol@LB@Unfolding Energy - mRNA 1.98532 kcal/mol@LB@Unfolding Energy - sRNA 0.591348 kcal/mol@LB@@LB@Position - mRNA 135 -- 158@LB@Position - sRNA 123 -- 153@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 166 -- 172@LB@
188	0.0218	0.3833	b3357	crp	-15.41	0.022266	196 -- 257	123 -- 172	cAMP-activated global transcription factor mediator of catabolite repression	_____________195_________________258\n_____________\|___________________\|\n5'-GAC...AUAAC____________UU_____ACCGC...AGG-3'\n______________CGCGCAUGGUGC__GGCAA\n______________\|:\|:\|\|\|\|:\|\|\|__\|\|\|\|\|\n______________GUGUGUACUACG__CCGUU\n3'-UUU...AGUCA____________U______CAGUU...UAG-5'\n_____________\|___________________\|\n_____________173_________________122\n	201 -- 207	161 -- 167	-26.7729 kcal/mol	8.17538 kcal/mol	7.52835 kcal/mol	195 258@LB@ \| \|@LB@5'-GAC...AUAAC UU ACCGC...AGG-3'@LB@ CGCGCAUGGUGC GGCAA@LB@ \|:\|:\|\|\|\|:\|\|\| \|\|\|\|\|@LB@ GUGUGUACUACG CCGUU@LB@3'-UUU...AGUCA U CAGUU...UAG-5'@LB@ \| \|@LB@ 173 122@LB@@LB@@LB@Energy -15.41 kcal/mol@LB@Hybridization Energy -26.7729 kcal/mol@LB@Unfolding Energy - mRNA 8.17538 kcal/mol@LB@Unfolding Energy - sRNA 7.52835 kcal/mol@LB@@LB@Position - mRNA 196 -- 257@LB@Position - sRNA 123 -- 172@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 161 -- 167@LB@
23	0.00185	0.2633	b3492	yhiN	-11.85	0.119629	108 -- 154	124 -- 161	putative oxidoreductase	_____________107_______________________________________155\n_____________\|_________________________________________\|\n5'-CGG...GGAAA_____G______U____AUAG____________________AUGUU...AUA-3'\n______________GGUUU_AUGCCA_UAUU_____GCGC_UGGUGC_GGCGGGU\n______________\|:\|\|\|_\|::\|\|\|_\|\|:\|_____:\|:\|_\|\|:\|\|\|_\|\|\|::\|\|\n______________CUAAA_UGUGGU_AUGA_____UGUG_ACUACG_CCGUUCA\n3'-UUU...GCCCA_____A______U____GUCAG____U______U_______GUUGA...UAG-5'\n_____________\|_________________________________________\|\n_____________162_______________________________________123\n	239 -- 245	153 -- 159	-35.0652 kcal/mol	11.4478 kcal/mol	11.9045 kcal/mol	107 155@LB@ \| \|@LB@5'-CGG...GGAAA G U AUAG AUGUU...AUA-3'@LB@ GGUUU AUGCCA UAUU GCGC UGGUGC GGCGGGU@LB@ \|:\|\|\| \|::\|\|\| \|\|:\| :\|:\| \|\|:\|\|\| \|\|\|::\|\|@LB@ CUAAA UGUGGU AUGA UGUG ACUACG CCGUUCA@LB@3'-UUU...GCCCA A U GUCAG U U GUUGA...UAG-5'@LB@ \| \|@LB@ 162 123@LB@@LB@@LB@Energy -11.85 kcal/mol@LB@Hybridization Energy -35.0652 kcal/mol@LB@Unfolding Energy - mRNA 11.4478 kcal/mol@LB@Unfolding Energy - sRNA 11.9045 kcal/mol@LB@@LB@Position - mRNA 108 -- 154@LB@Position - sRNA 124 -- 161@LB@Position Seed - mRNA 239 -- 245@LB@Position Seed - sRNA 153 -- 159@LB@
75	0.007778	0.3458	b0766	ybhA	-9.49	0.341100	208 -- 245	124 -- 172	pyridoxal phosphate (PLP) phosphatase	_____________207___________________________246\n_____________\|_____________________________\|\n5'-GAA...UGGAA_______UG________________U___UCUCG...GCG-3'\n______________UACGCCA__AC_CA__CACGCGUGA_UGC\n______________\|\|\|:\|\|\|__\|\|_\|\|__\|\|\|:\|:\|\|\|_\|\|\|\n______________AUGUGGU__UG_GU__GUGUGUACU_ACG\n3'-UUU...CUAAA_______UA__A__CA_____________UCCGU...UAG-5'\n_____________\|_____________________________\|\n_____________173___________________________123\n	195 -- 201	182 -- 188	-28.0817 kcal/mol	7.23992 kcal/mol	7.72227 kcal/mol	207 246@LB@ \| \|@LB@5'-GAA...UGGAA UG U UCUCG...GCG-3'@LB@ UACGCCA AC CA CACGCGUGA UGC@LB@ \|\|\|:\|\|\| \|\| \|\| \|\|\|:\|:\|\|\| \|\|\|@LB@ AUGUGGU UG GU GUGUGUACU ACG@LB@3'-UUU...CUAAA UA A CA UCCGU...UAG-5'@LB@ \| \|@LB@ 173 123@LB@@LB@@LB@Energy -9.49 kcal/mol@LB@Hybridization Energy -28.0817 kcal/mol@LB@Unfolding Energy - mRNA 7.23992 kcal/mol@LB@Unfolding Energy - sRNA 7.72227 kcal/mol@LB@@LB@Position - mRNA 208 -- 245@LB@Position - sRNA 124 -- 172@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 182 -- 188@LB@
81	0.008673	0.3575	b1683	sufB	-9.84	0.294156	211 -- 257	124 -- 171	component of SufBCD Fe-S cluster assembly scaffold	_____________210_______________258\n_____________\|_________________\|\n5'-GUU...AAUCA________AAUU_____ACCCU...AGC-3'\n______________GAUAUUCA____UCACA\n______________:\|\|\|:\|\|\|____\|\|\|\|\|\n______________UUAUGAGU____AGUGU\n3'-UUU...UGUGG________C________GUACU...UAG-5'\n_____________\|_________________\|\n_____________172_______________123\n	127 -- 133	175 -- 181	-8.93573 kcal/mol	1.46068 kcal/mol	0.409577 kcal/mol	210 258@LB@ \| \|@LB@5'-GUU...AAUCA AAUU ACCCU...AGC-3'@LB@ GAUAUUCA UCACA@LB@ :\|\|\|:\|\|\| \|\|\|\|\|@LB@ UUAUGAGU AGUGU@LB@3'-UUU...UGUGG C GUACU...UAG-5'@LB@ \| \|@LB@ 172 123@LB@@LB@@LB@Energy -9.84 kcal/mol@LB@Hybridization Energy -8.93573 kcal/mol@LB@Unfolding Energy - mRNA 1.46068 kcal/mol@LB@Unfolding Energy - sRNA 0.409577 kcal/mol@LB@@LB@Position - mRNA 211 -- 257@LB@Position - sRNA 124 -- 171@LB@Position Seed - mRNA 127 -- 133@LB@Position Seed - sRNA 175 -- 181@LB@
122	0.01342	0.3575	b4087	alsA	-13.14	0.065197	11 -- 23	124 -- 136	D-allose ABC transporter ATPase	_____________10___________24\n_____________\|____________\|\n5'-AAU...GGCAA____________GGAUA...UAC-3'\n______________GGUUAUCCCGCU\n______________\|\|\|:\|\|\|\|\|:\|\|\n______________CCAGUAGGGUGA\n3'-UUU...UAACG____________CGUAG...UAG-5'\n_____________\|____________\|\n_____________137__________123\n	16 -- 22	125 -- 131	-19.4238 kcal/mol	3.3353 kcal/mol	6.53419 kcal/mol	10 24@LB@ \| \|@LB@5'-AAU...GGCAA GGAUA...UAC-3'@LB@ GGUUAUCCCGCU@LB@ \|\|\|:\|\|\|\|\|:\|\|@LB@ CCAGUAGGGUGA@LB@3'-UUU...UAACG CGUAG...UAG-5'@LB@ \| \|@LB@ 137 123@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -19.4238 kcal/mol@LB@Unfolding Energy - mRNA 3.3353 kcal/mol@LB@Unfolding Energy - sRNA 6.53419 kcal/mol@LB@@LB@Position - mRNA 11 -- 23@LB@Position - sRNA 124 -- 136@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 125 -- 131@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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