Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
37	0.00305	0.2773	b4084	alsK	-13.69	0.050122	28 -- 80	50 -- 101	D-allose kinase		_____________27__________________________________________________________81\n_____________\|___________________________________________________________\|\n5'-GCG...UACGA_____AG________G_________________________A__AAAAA___A_G____CAGGG...AAA-3'\n______________AAUUG__GU_GGACG_UU__CC__UGCAACCAG_GCAACUU_CG_____CUG_U_GCGG\n______________\|\|\|:\|__\|\|_\|\|\|\|\|_\|\|__\|\|__:\|\|\|\|\|\|\|\|_:\|\|\|\|\|\|_\|\|_____\|\|\|_\|_\|\|\|\|\n______________UUAGC__CA_CCUGC_AA__GG__GCGUUGGUC_UGUUGAA_GC_____GAC_A_CGCC\n3'-UUU...ACCAA_____AA__C_____G__UU__UU_________G____________________G____AUUUU...UAG-5'\n_____________\|___________________________________________________________\|\n_____________102_________________________________________________________49\n	56 -- 62	61 -- 67	-47.5504 kcal/mol	15.3524 kcal/mol	25.4279 kcal/mol	27 81@LB@ \| \|@LB@5'-GCG...UACGA AG G A AAAAA A G CAGGG...AAA-3'@LB@ AAUUG GU GGACG UU CC UGCAACCAG GCAACUU CG CUG U GCGG@LB@ \|\|\|:\| \|\| \|\|\|\|\| \|\| \|\| :\|\|\|\|\|\|\|\| :\|\|\|\|\|\| \|\| \|\|\| \| \|\|\|\|@LB@ UUAGC CA CCUGC AA GG GCGUUGGUC UGUUGAA GC GAC A CGCC@LB@3'-UUU...ACCAA AA C G UU UU G G AUUUU...UAG-5'@LB@ \| \|@LB@ 102 49@LB@@LB@@LB@Energy -13.69 kcal/mol@LB@Hybridization Energy -47.5504 kcal/mol@LB@Unfolding Energy - mRNA 15.3524 kcal/mol@LB@Unfolding Energy - sRNA 25.4279 kcal/mol@LB@@LB@Position - mRNA 28 -- 80@LB@Position - sRNA 50 -- 101@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 61 -- 67@LB@
129	0.01414	0.3575	b3579	yiaO	-11.03	0.174508	249 -- 273	50 -- 76	2 3-diketo-L-gulonate-binding periplasmic protein		_____________248_________________________274\n_____________\|___________________________\|\n5'-ACU...CGUCC____________C__UA__________UUGUU...AAA-3'\n______________UGGUUUUAUAUU_AU__UUAGUCGUAU\n______________\|\|:\|\|\|\|\|:\|::_\|\|__\|:\|\|\|\|::\|:\n______________ACUAAAAUGUGG_UA__AGUCAGUGUG\n3'-UUU...CGCCC____________U__UG__________UACUA...UAG-5'\n_____________\|___________________________\|\n_____________77__________________________49\n	132 -- 138	168 -- 174	-19.0823 kcal/mol	5.4875 kcal/mol	5.03533 kcal/mol	248 274@LB@ \| \|@LB@5'-ACU...CGUCC C UA UUGUU...AAA-3'@LB@ UGGUUUUAUAUU AU UUAGUCGUAU@LB@ \|\|:\|\|\|\|\|:\|:: \|\| \|:\|\|\|\|::\|:@LB@ ACUAAAAUGUGG UA AGUCAGUGUG@LB@3'-UUU...CGCCC U UG UACUA...UAG-5'@LB@ \| \|@LB@ 77 49@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -19.0823 kcal/mol@LB@Unfolding Energy - mRNA 5.4875 kcal/mol@LB@Unfolding Energy - sRNA 5.03533 kcal/mol@LB@@LB@Position - mRNA 249 -- 273@LB@Position - sRNA 50 -- 76@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 168 -- 174@LB@
181	0.0209	0.3833	b2233	yfaL	-9.66	0.317681	275 -- 285	50 -- 60	adhesin		_____________274______________286\n_____________\|________________\|\n5'-UAU...UCUCU___AA__G________CGGUC...CAU-3'\n______________GCU__CG_UGUCGCGG\n______________:\|\|__\|\|_\|\|\|\|\|\|\|\|\n______________UGA__GC_ACAGCGCC\n3'-UUU...CGUGU___A___G________AUUUU...UAG-5'\n_____________\|________________\|\n_____________61_______________49\n	279 -- 285	50 -- 56	-20.2435 kcal/mol	4.65708 kcal/mol	6.1451 kcal/mol	274 286@LB@ \| \|@LB@5'-UAU...UCUCU AA G CGGUC...CAU-3'@LB@ GCU CG UGUCGCGG@LB@ :\|\| \|\| \|\|\|\|\|\|\|\|@LB@ UGA GC ACAGCGCC@LB@3'-UUU...CGUGU A G AUUUU...UAG-5'@LB@ \| \|@LB@ 61 49@LB@@LB@@LB@Energy -9.66 kcal/mol@LB@Hybridization Energy -20.2435 kcal/mol@LB@Unfolding Energy - mRNA 4.65708 kcal/mol@LB@Unfolding Energy - sRNA 6.1451 kcal/mol@LB@@LB@Position - mRNA 275 -- 285@LB@Position - sRNA 50 -- 60@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 50 -- 56@LB@
4	0.0001068	0.08442	b2069	yegD	-18.64	0.004982	22 -- 145	51 -- 187	Hsp70 chaperone family protein		_____________21_________________146\n_____________\|__________________\|\n5'-CAC...ACAUC__________A_______CGCUU...UGC-3'\n______________GCAUCUCCUU__UUCAUC\n______________\|\|\|:\|:\|\|\|\|__\|\|\|\|\|\|\n______________CGUGGGGGAA__AAGUAG\n3'-UUU...UACCC__________CG______-5'\n_____________\|_________________\|\n_____________188_______________51\n	72 -- 78	9 -- 15	-21.4418 kcal/mol	1.05479 kcal/mol	7.2533 kcal/mol	21 146@LB@ \| \|@LB@5'-CAC...ACAUC A CGCUU...UGC-3'@LB@ GCAUCUCCUU UUCAUC@LB@ \|\|\|:\|:\|\|\|\| \|\|\|\|\|\|@LB@ CGUGGGGGAA AAGUAG@LB@3'-UUU...UACCC CG -5'@LB@ \| \|@LB@ 188 51@LB@@LB@@LB@Energy -18.64 kcal/mol@LB@Hybridization Energy -21.4418 kcal/mol@LB@Unfolding Energy - mRNA 1.05479 kcal/mol@LB@Unfolding Energy - sRNA 7.2533 kcal/mol@LB@@LB@Position - mRNA 22 -- 145@LB@Position - sRNA 51 -- 187@LB@Position Seed - mRNA 72 -- 78@LB@Position Seed - sRNA 9 -- 15@LB@
16	0.0008966	0.1886	b0807	rlmF	-16.29	0.014793	117 -- 160	51 -- 97	23S rRNA m(6)A1618 methyltransferase SAM-dependent		_____________116_____________________________________________161\n_____________\|_______________________________________________\|\n5'-AUU...UUAAG___U_____AA______A_______________U__A_C________UUAUG...UCC-3'\n______________GUU_UGGGG____UGAU_AACGCAGCCAG_AUA_UU_C_CUGUCGCG\n______________\|\|\|_\|\|\|\|:____\|:\|:_\|\|\|\|\|\|:\|\|\|\|_\|:\|_:\|_\|_\|\|\|\|\|\|\|\|\n______________CAA_ACCCU____AUUG_UUGCGUUGGUC_UGU_GA_G_GACAGCGC\n3'-UUU...AUUAG___C_____GCGA____G___________G___U__A_C________CAUUU...UAG-5'\n_____________\|_______________________________________________\|\n_____________98______________________________________________50\n	154 -- 160	51 -- 57	-44.7843 kcal/mol	10.0752 kcal/mol	25.3667 kcal/mol	116 161@LB@ \| \|@LB@5'-AUU...UUAAG U AA A U A C UUAUG...UCC-3'@LB@ GUU UGGGG UGAU AACGCAGCCAG AUA UU C CUGUCGCG@LB@ \|\|\| \|\|\|\|: \|:\|: \|\|\|\|\|\|:\|\|\|\| \|:\| :\| \| \|\|\|\|\|\|\|\|@LB@ CAA ACCCU AUUG UUGCGUUGGUC UGU GA G GACAGCGC@LB@3'-UUU...AUUAG C GCGA G G U A C CAUUU...UAG-5'@LB@ \| \|@LB@ 98 50@LB@@LB@@LB@Energy -16.29 kcal/mol@LB@Hybridization Energy -44.7843 kcal/mol@LB@Unfolding Energy - mRNA 10.0752 kcal/mol@LB@Unfolding Energy - sRNA 25.3667 kcal/mol@LB@@LB@Position - mRNA 117 -- 160@LB@Position - sRNA 51 -- 97@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 51 -- 57@LB@
69	0.006739	0.3286	b2265	menF	-9.03	0.410924	65 -- 108	52 -- 101	isochorismate synthase 2		_____________64__________109\n_____________\|___________\|\n5'-ACU...CGGGU___________CUUUC...CUC-3'\n______________UAUCGAUAUUC\n______________:\|:\|:\|\|\|:\|\|\n______________GUGGUUAUGAG\n3'-UUU...AAAAU___________UCAGU...UAG-5'\n_____________\|___________\|\n_____________102_________51\n	285 -- 291	176 -- 182	-9.38108 kcal/mol	0.14396 kcal/mol	3.38772 kcal/mol	64 109@LB@ \| \|@LB@5'-ACU...CGGGU CUUUC...CUC-3'@LB@ UAUCGAUAUUC@LB@ :\|:\|:\|\|\|:\|\|@LB@ GUGGUUAUGAG@LB@3'-UUU...AAAAU UCAGU...UAG-5'@LB@ \| \|@LB@ 102 51@LB@@LB@@LB@Energy -9.03 kcal/mol@LB@Hybridization Energy -9.38108 kcal/mol@LB@Unfolding Energy - mRNA 0.14396 kcal/mol@LB@Unfolding Energy - sRNA 3.38772 kcal/mol@LB@@LB@Position - mRNA 65 -- 108@LB@Position - sRNA 52 -- 101@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 176 -- 182@LB@
182	0.02094	0.3833	b0248	yafX	-12.67	0.081205	189 -- 235	52 -- 92	CP4-6 prophage; uncharacterized protein	b2643	_____________188______________________236\n_____________\|________________________\|\n5'-CCG...ACACA___G_________C____ACC___UAACC...GGC-3'\n______________GAC_CAGCACGAC_UCGC___CGC\n______________:\|\|_\|\|\|\|\|\|:\|\|_\|\|\|\|___\|\|\|\n______________UUG_GUCGUGUUG_AGCG___GCG\n3'-UUU...UUGCG_____________A____ACA___CCAUU...UAG-5'\n_____________\|________________________\|\n_____________93_______________________51\n	218 -- 224	63 -- 69	-27.2596 kcal/mol	3.0161 kcal/mol	15.5954 kcal/mol	188 236@LB@ \| \|@LB@5'-CCG...ACACA G C ACC UAACC...GGC-3'@LB@ GAC CAGCACGAC UCGC CGC@LB@ :\|\| \|\|\|\|\|\|:\|\| \|\|\|\| \|\|\|@LB@ UUG GUCGUGUUG AGCG GCG@LB@3'-UUU...UUGCG A ACA CCAUU...UAG-5'@LB@ \| \|@LB@ 93 51@LB@@LB@@LB@Energy -12.67 kcal/mol@LB@Hybridization Energy -27.2596 kcal/mol@LB@Unfolding Energy - mRNA 3.0161 kcal/mol@LB@Unfolding Energy - sRNA 15.5954 kcal/mol@LB@@LB@Position - mRNA 189 -- 235@LB@Position - sRNA 52 -- 92@LB@Position Seed - mRNA 218 -- 224@LB@Position Seed - sRNA 63 -- 69@LB@
88	0.009956	0.3575	b2672	ygaM	-12.38	0.093122	193 -- 231	56 -- 92	putative membrane-anchored DUF883 family ribosome-binding protein		_____________192______232\n_____________\|________\|\n5'-AAC...UGAAC________AGAAA...AGU-3'\n______________CUCAGUUA\n______________\|\|\|\|\|\|:\|\n______________GAGUCAGU\n3'-UUU...GUUAU________GUGUA...UAG-5'\n_____________\|________\|\n_____________93_______55\n	142 -- 148	171 -- 177	-10.1878 kcal/mol	1.87036 kcal/mol	0.207782 kcal/mol	192 232@LB@ \| \|@LB@5'-AAC...UGAAC AGAAA...AGU-3'@LB@ CUCAGUUA@LB@ \|\|\|\|\|\|:\|@LB@ GAGUCAGU@LB@3'-UUU...GUUAU GUGUA...UAG-5'@LB@ \| \|@LB@ 93 55@LB@@LB@@LB@Energy -12.38 kcal/mol@LB@Hybridization Energy -10.1878 kcal/mol@LB@Unfolding Energy - mRNA 1.87036 kcal/mol@LB@Unfolding Energy - sRNA 0.207782 kcal/mol@LB@@LB@Position - mRNA 193 -- 231@LB@Position - sRNA 56 -- 92@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 171 -- 177@LB@
104	0.01113	0.3575	b2108	yehA	-14.54	0.033608	284 -- 300	56 -- 72	putative fimbrial-like adhesin protein		_____________283_____________300\n_____________\|_______________\|\n5'-GUU...CUGUU___________G____-3'\n______________CAGCACAACUU_GUUG\n______________\|\|\|\|\|\|\|\|\|\|\|_\|:\|\|\n______________GUCGUGUUGAA_CGAC\n3'-UUU...CGUUG___________G____AGCGC...UAG-5'\n_____________\|________________\|\n_____________73_______________55\n	289 -- 295	61 -- 67	-24.1215 kcal/mol	2.36332 kcal/mol	11.1531 kcal/mol	283 300@LB@ \| \|@LB@5'-GUU...CUGUU G -3'@LB@ CAGCACAACUU GUUG@LB@ \|\|\|\|\|\|\|\|\|\|\| \|:\|\|@LB@ GUCGUGUUGAA CGAC@LB@3'-UUU...CGUUG G AGCGC...UAG-5'@LB@ \| \|@LB@ 73 55@LB@@LB@@LB@Energy -14.54 kcal/mol@LB@Hybridization Energy -24.1215 kcal/mol@LB@Unfolding Energy - mRNA 2.36332 kcal/mol@LB@Unfolding Energy - sRNA 11.1531 kcal/mol@LB@@LB@Position - mRNA 284 -- 300@LB@Position - sRNA 56 -- 72@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 61 -- 67@LB@
70	0.00686	0.3298	b0761	modE	-10.05	0.269682	248 -- 271	57 -- 77	transcriptional repressor for the molybdenum transport operon modABC		_____________247______272\n_____________\|________\|\n5'-ACC...GAAAA________CUUUU...CCA-3'\n______________GCACUCCC\n______________\|\|\|\|:\|\|\|\n______________CGUGGGGG\n3'-UUU...UACCC________AACGA...UAG-5'\n_____________\|________\|\n_____________78_______56\n	126 -- 132	11 -- 17	-14.7879 kcal/mol	0.633098 kcal/mol	5.13224 kcal/mol	247 272@LB@ \| \|@LB@5'-ACC...GAAAA CUUUU...CCA-3'@LB@ GCACUCCC@LB@ \|\|\|\|:\|\|\|@LB@ CGUGGGGG@LB@3'-UUU...UACCC AACGA...UAG-5'@LB@ \| \|@LB@ 78 56@LB@@LB@@LB@Energy -10.05 kcal/mol@LB@Hybridization Energy -14.7879 kcal/mol@LB@Unfolding Energy - mRNA 0.633098 kcal/mol@LB@Unfolding Energy - sRNA 5.13224 kcal/mol@LB@@LB@Position - mRNA 248 -- 271@LB@Position - sRNA 57 -- 77@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 11 -- 17@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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