Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
142	0.02758	0.6177	b3186	rplU	-10.01	0.040887	186 -- 200	38 -- 52	50S ribosomal subunit protein L21	_____________185_____________201\n_____________\|_______________\|\n5'-UUA...GCGCG____C__________AUGUA...UUG-3'\n______________GAAG_GGAGUUUUAU\n______________:\|\|\|_\|\|\|:\|\|\|:\|\|\n______________UUUC_CCUUAAAGUA\n3'-UUU...GGCAG____U__________AAAAA...ACA-5'\n_____________\|_______________\|\n_____________53______________37\n	194 -- 200	38 -- 44	-13.3778 kcal/mol	2.29537 kcal/mol	1.0693 kcal/mol	185 201@LB@ \| \|@LB@5'-UUA...GCGCG C AUGUA...UUG-3'@LB@ GAAG GGAGUUUUAU@LB@ :\|\|\| \|\|\|:\|\|\|:\|\|@LB@ UUUC CCUUAAAGUA@LB@3'-UUU...GGCAG U AAAAA...ACA-5'@LB@ \| \|@LB@ 53 37@LB@@LB@@LB@Energy -10.01 kcal/mol@LB@Hybridization Energy -13.3778 kcal/mol@LB@Unfolding Energy - mRNA 2.29537 kcal/mol@LB@Unfolding Energy - sRNA 1.0693 kcal/mol@LB@@LB@Position - mRNA 186 -- 200@LB@Position - sRNA 38 -- 52@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 38 -- 44@LB@
100	0.01963	0.6177	b4001	yjaH	-9.93	0.042585	215 -- 230	39 -- 53	DUF1481 family putative lipoprotein	_____________214______________231\n_____________\|________________\|\n5'-UGG...UUUAA________U_______CCCCU...AUA-3'\n______________CGAAGGGG_GGUUUCA\n______________\|:\|\|\|:\|\|_::\|\|\|\|\|\n______________GUUUCUCC_UUAAAGU\n3'-UUU...GGGCA________________AAAAA...ACA-5'\n_____________\|________________\|\n_____________54_______________38\n	216 -- 222	46 -- 52	-15.2589 kcal/mol	4.19806 kcal/mol	1.13439 kcal/mol	214 231@LB@ \| \|@LB@5'-UGG...UUUAA U CCCCU...AUA-3'@LB@ CGAAGGGG GGUUUCA@LB@ \|:\|\|\|:\|\| ::\|\|\|\|\|@LB@ GUUUCUCC UUAAAGU@LB@3'-UUU...GGGCA AAAAA...ACA-5'@LB@ \| \|@LB@ 54 38@LB@@LB@@LB@Energy -9.93 kcal/mol@LB@Hybridization Energy -15.2589 kcal/mol@LB@Unfolding Energy - mRNA 4.19806 kcal/mol@LB@Unfolding Energy - sRNA 1.13439 kcal/mol@LB@@LB@Position - mRNA 215 -- 230@LB@Position - sRNA 39 -- 53@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 46 -- 52@LB@
82	0.01753	0.6177	b2204	napH	-9.88	0.043521	152 -- 162	42 -- 52	ferredoxin-type protein	_____________151_________163\n_____________\|___________\|\n5'-CAC...UGGCG___________AGGUC...UCU-3'\n______________AAAGGGGAGUU\n______________\|\|\|\|:\|\|\|:\|\|\n______________UUUCUCCUUAA\n3'-UUU...GGCAG___________AGUAA...ACA-5'\n_____________\|___________\|\n_____________53__________41\n	156 -- 162	42 -- 48	-12.4472 kcal/mol	1.70007 kcal/mol	0.866923 kcal/mol	151 163@LB@ \| \|@LB@5'-CAC...UGGCG AGGUC...UCU-3'@LB@ AAAGGGGAGUU@LB@ \|\|\|\|:\|\|\|:\|\|@LB@ UUUCUCCUUAA@LB@3'-UUU...GGCAG AGUAA...ACA-5'@LB@ \| \|@LB@ 53 41@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -12.4472 kcal/mol@LB@Unfolding Energy - mRNA 1.70007 kcal/mol@LB@Unfolding Energy - sRNA 0.866923 kcal/mol@LB@@LB@Position - mRNA 152 -- 162@LB@Position - sRNA 42 -- 52@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 42 -- 48@LB@
42	0.008125	0.6129	b2431	yfeX	-9.88	0.043594	266 -- 278	44 -- 56	porphyrinogen oxidase cytoplasmic	_____________265___________279\n_____________\|_____________\|\n5'-AUU...GCCAA___G_________GUUGA...CCA-3'\n______________CGU_AAAGGGGAA\n______________\|\|\|_\|\|\|\|:\|\|\|\|\n______________GCA_UUUCUCCUU\n3'-UUU...ACCGG___G_________AAAGU...ACA-5'\n_____________\|_____________\|\n_____________57____________43\n	272 -- 278	44 -- 50	-15.4775 kcal/mol	4.65199 kcal/mol	0.948902 kcal/mol	265 279@LB@ \| \|@LB@5'-AUU...GCCAA G GUUGA...CCA-3'@LB@ CGU AAAGGGGAA@LB@ \|\|\| \|\|\|\|:\|\|\|\|@LB@ GCA UUUCUCCUU@LB@3'-UUU...ACCGG G AAAGU...ACA-5'@LB@ \| \|@LB@ 57 43@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -15.4775 kcal/mol@LB@Unfolding Energy - mRNA 4.65199 kcal/mol@LB@Unfolding Energy - sRNA 0.948902 kcal/mol@LB@@LB@Position - mRNA 266 -- 278@LB@Position - sRNA 44 -- 56@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 44 -- 50@LB@
189	0.03933	0.6177	b2461	eutP	-9.78	0.045635	227 -- 240	44 -- 56	putative P-loop NTPase ethanolamine utilization protein	_____________226____________241\n_____________\|______________\|\n5'-AAA...GGUAG________C_____AAACA...CCG-3'\n______________CGUCGGGG_AGGAA\n______________\|\|\|\|:::\|_\|\|\|\|\|\n______________GCAGUUUC_UCCUU\n3'-UUU...ACCGG______________AAAGU...ACA-5'\n_____________\|______________\|\n_____________57_____________43\n	228 -- 234	49 -- 55	-15.0697 kcal/mol	4.34178 kcal/mol	0.948902 kcal/mol	226 241@LB@ \| \|@LB@5'-AAA...GGUAG C AAACA...CCG-3'@LB@ CGUCGGGG AGGAA@LB@ \|\|\|\|:::\| \|\|\|\|\|@LB@ GCAGUUUC UCCUU@LB@3'-UUU...ACCGG AAAGU...ACA-5'@LB@ \| \|@LB@ 57 43@LB@@LB@@LB@Energy -9.78 kcal/mol@LB@Hybridization Energy -15.0697 kcal/mol@LB@Unfolding Energy - mRNA 4.34178 kcal/mol@LB@Unfolding Energy - sRNA 0.948902 kcal/mol@LB@@LB@Position - mRNA 227 -- 240@LB@Position - sRNA 44 -- 56@LB@Position Seed - mRNA 228 -- 234@LB@Position Seed - sRNA 49 -- 55@LB@
44	0.008636	0.6129	b3317	rplB	-9.78	0.045660	184 -- 194	43 -- 55	50S ribosomal subunit protein L2	_____________183___________195\n_____________\|_____________\|\n5'-GCA...AGUAA_____________AAUAC...UGC-3'\n______________GUC__GGAGGAGU\n______________\|\|\|__:\|\|\|\|\|:\|\n______________CAG__UCUCCUUA\n3'-UUU...CCGGG___UU________AAGUA...ACA-5'\n_____________\|_____________\|\n_____________56____________42\n	188 -- 194	43 -- 49	-13.3143 kcal/mol	2.58995 kcal/mol	0.946481 kcal/mol	183 195@LB@ \| \|@LB@5'-GCA...AGUAA AAUAC...UGC-3'@LB@ GUC GGAGGAGU@LB@ \|\|\| :\|\|\|\|\|:\|@LB@ CAG UCUCCUUA@LB@3'-UUU...CCGGG UU AAGUA...ACA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -9.78 kcal/mol@LB@Hybridization Energy -13.3143 kcal/mol@LB@Unfolding Energy - mRNA 2.58995 kcal/mol@LB@Unfolding Energy - sRNA 0.946481 kcal/mol@LB@@LB@Position - mRNA 184 -- 194@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 43 -- 49@LB@
118	0.0238	0.6177	b3321	rpsJ	-9.75	0.046314	151 -- 177	30 -- 55	30S ribosomal subunit protein S10	_____________150___________________________178\n_____________\|_____________________________\|\n5'-UUC...AAUGG___U_________AA_______CG_____AAAAU...GUG-3'\n______________GUC___GAGGAGU__UCAUUUU__UUUAU\n______________\|\|\|___\|\|\|\|\|:\|__\|\|\|\|\|\|\|__\|\|\|\|\|\n______________CAG___CUCCUUA__AGUAAAA__AAAUA\n3'-UUU...CCGGG___UUU_______A_______________AUAAU...ACA-5'\n_____________\|_____________________________\|\n_____________56____________________________29\n	164 -- 170	35 -- 41	-16.2369 kcal/mol	5.30163 kcal/mol	1.18773 kcal/mol	150 178@LB@ \| \|@LB@5'-UUC...AAUGG U AA CG AAAAU...GUG-3'@LB@ GUC GAGGAGU UCAUUUU UUUAU@LB@ \|\|\| \|\|\|\|\|:\| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ CAG CUCCUUA AGUAAAA AAAUA@LB@3'-UUU...CCGGG UUU A AUAAU...ACA-5'@LB@ \| \|@LB@ 56 29@LB@@LB@@LB@Energy -9.75 kcal/mol@LB@Hybridization Energy -16.2369 kcal/mol@LB@Unfolding Energy - mRNA 5.30163 kcal/mol@LB@Unfolding Energy - sRNA 1.18773 kcal/mol@LB@@LB@Position - mRNA 151 -- 177@LB@Position - sRNA 30 -- 55@LB@Position Seed - mRNA 164 -- 170@LB@Position Seed - sRNA 35 -- 41@LB@
32	0.006131	0.6129	b2585	pssA	-9.74	0.046374	32 -- 41	45 -- 54	phosphatidylserine synthase; CDP-diacylglycerol-serine O-phosphatidyltransferase	_____________31_________42\n_____________\|__________\|\n5'-CGC...CCAGC__________GAUCG...CCG-3'\n______________UCGAAGAGGG\n______________\|\|:\|\|\|\|\|\|:\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	35 -- 41	45 -- 51	-13.2862 kcal/mol	2.61225 kcal/mol	0.929144 kcal/mol	31 42@LB@ \| \|@LB@5'-CGC...CCAGC GAUCG...CCG-3'@LB@ UCGAAGAGGG@LB@ \|\|:\|\|\|\|\|\|:@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -13.2862 kcal/mol@LB@Unfolding Energy - mRNA 2.61225 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 32 -- 41@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 45 -- 51@LB@
65	0.01408	0.6177	b3176	glmM	-9.74	0.046438	133 -- 141	48 -- 56	phosphoglucosamine mutase	_____________132_______142\n_____________\|_________\|\n5'-GUU...CAUGA_________AACCG...CGG-3'\n______________CGUCAAAGA\n______________\|\|\|\|\|\|\|\|\|\n______________GCAGUUUCU\n3'-UUU...ACCGG_________CCUUA...ACA-5'\n_____________\|_________\|\n_____________57________47\n	135 -- 141	48 -- 54	-11.8395 kcal/mol	1.20122 kcal/mol	0.896433 kcal/mol	132 142@LB@ \| \|@LB@5'-GUU...CAUGA AACCG...CGG-3'@LB@ CGUCAAAGA@LB@ \|\|\|\|\|\|\|\|\|@LB@ GCAGUUUCU@LB@3'-UUU...ACCGG CCUUA...ACA-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -11.8395 kcal/mol@LB@Unfolding Energy - mRNA 1.20122 kcal/mol@LB@Unfolding Energy - sRNA 0.896433 kcal/mol@LB@@LB@Position - mRNA 133 -- 141@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 48 -- 54@LB@
75	0.01577	0.6177	b1345	intR	-9.71	0.047033	139 -- 151	42 -- 53	Rac prophage; integrase	_____________138___________152\n_____________\|_____________\|\n5'-UAA...GACAG____C________AUCUG...GGG-3'\n______________CAAA_GAGGGAUU\n______________\|\|\|\|_\|\|\|\|:\|\|\|\n______________GUUU_CUCCUUAA\n3'-UUU...GGGCA_____________AGUAA...ACA-5'\n_____________\|_____________\|\n_____________54____________41\n	145 -- 151	42 -- 48	-12.4741 kcal/mol	1.8255 kcal/mol	0.933904 kcal/mol	138 152@LB@ \| \|@LB@5'-UAA...GACAG C AUCUG...GGG-3'@LB@ CAAA GAGGGAUU@LB@ \|\|\|\| \|\|\|\|:\|\|\|@LB@ GUUU CUCCUUAA@LB@3'-UUU...GGGCA AGUAA...ACA-5'@LB@ \| \|@LB@ 54 41@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -12.4741 kcal/mol@LB@Unfolding Energy - mRNA 1.8255 kcal/mol@LB@Unfolding Energy - sRNA 0.933904 kcal/mol@LB@@LB@Position - mRNA 139 -- 151@LB@Position - sRNA 42 -- 53@LB@Position Seed - mRNA 145 -- 151@LB@Position Seed - sRNA 42 -- 48@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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