Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
128	0.02532	0.6177	b3642	pyrE	-8.06	0.101423	72 -- 92	43 -- 68	orotate phosphoribosyltransferase	_____________71_________________________93\n_____________\|__________________________\|\n5'-CGG...CUUGU_G___C____________________CGAAU...AUU-3'\n______________U_GCU__UGGCCCG____AGGGGAAU\n______________\|_\|\|\|__\|\|\|\|\|\|\|____\|\|:\|\|\|\|\|\n______________A_CGA__ACCGGGC____UCUCCUUA\n3'-UUU...GUUAU_G___UA_______AGUU________AAGUA...ACA-5'\n_____________\|__________________________\|\n_____________69_________________________42\n	86 -- 92	43 -- 49	-26.5136 kcal/mol	7.12642 kcal/mol	11.3306 kcal/mol	71 93@LB@ \| \|@LB@5'-CGG...CUUGU G C CGAAU...AUU-3'@LB@ U GCU UGGCCCG AGGGGAAU@LB@ \| \|\|\| \|\|\|\|\|\|\| \|\|:\|\|\|\|\|@LB@ A CGA ACCGGGC UCUCCUUA@LB@3'-UUU...GUUAU G UA AGUU AAGUA...ACA-5'@LB@ \| \|@LB@ 69 42@LB@@LB@@LB@Energy -8.06 kcal/mol@LB@Hybridization Energy -26.5136 kcal/mol@LB@Unfolding Energy - mRNA 7.12642 kcal/mol@LB@Unfolding Energy - sRNA 11.3306 kcal/mol@LB@@LB@Position - mRNA 72 -- 92@LB@Position - sRNA 43 -- 68@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 43 -- 49@LB@
116	0.02318	0.6177	b1726	yniB	-7.96	0.105879	188 -- 195	47 -- 54	putative inner membrane protein	_____________187______196\n_____________\|________\|\n5'-AAG...CUUAA________AACUC...UGA-3'\n______________UCAAGGAG\n______________\|\|\|\|:\|\|\|\n______________AGUUUCUC\n3'-UUU...CGGGC________CUUAA...ACA-5'\n_____________\|________\|\n_____________55_______46\n	189 -- 195	47 -- 53	-9.99546 kcal/mol	1.13532 kcal/mol	0.897871 kcal/mol	187 196@LB@ \| \|@LB@5'-AAG...CUUAA AACUC...UGA-3'@LB@ UCAAGGAG@LB@ \|\|\|\|:\|\|\|@LB@ AGUUUCUC@LB@3'-UUU...CGGGC CUUAA...ACA-5'@LB@ \| \|@LB@ 55 46@LB@@LB@@LB@Energy -7.96 kcal/mol@LB@Hybridization Energy -9.99546 kcal/mol@LB@Unfolding Energy - mRNA 1.13532 kcal/mol@LB@Unfolding Energy - sRNA 0.897871 kcal/mol@LB@@LB@Position - mRNA 188 -- 195@LB@Position - sRNA 47 -- 54@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 47 -- 53@LB@
199	0.0417	0.6177	b4070	nrfA	-7.95	0.106497	2 -- 16	44 -- 55	nitrite reductase formate-dependent cytochrome	_____________1_______________17\n_____________\|_______________\|\n__________5'-U___GAA_________GAUAC...CAA-3'\n______________GUU___AAAGAGGAA\n______________\|\|:___\|\|\|\|\|\|\|\|\|\n______________CAG___UUUCUCCUU\n3'-UUU...CCGGG_______________AAAGU...ACA-5'\n_____________\|_______________\|\n_____________56______________43\n	10 -- 16	44 -- 50	-11.2989 kcal/mol	2.40804 kcal/mol	0.941432 kcal/mol	1 17@LB@ \| \|@LB@ 5'-U GAA GAUAC...CAA-3'@LB@ GUU AAAGAGGAA@LB@ \|\|: \|\|\|\|\|\|\|\|\|@LB@ CAG UUUCUCCUU@LB@3'-UUU...CCGGG AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -7.95 kcal/mol@LB@Hybridization Energy -11.2989 kcal/mol@LB@Unfolding Energy - mRNA 2.40804 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 2 -- 16@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 44 -- 50@LB@
92	0.01873	0.6177	b2297	pta	-7.94	0.106741	187 -- 219	22 -- 52	phosphate acetyltransferase	_____________186_______________________________220\n_____________\|_________________________________\|\n5'-CGC...UAACG________UAAAC___GUCCCG___________UGAUC...GUC-3'\n______________AAAGAGGA_____CGU______UAUUAUUAUGC\n______________\|\|\|\|\|\|\|\|_____\|:\|______\|\|\|\|\|\|\|\|\|\|\|\n______________UUUCUCCU_____GUA______AUAAUAAUACG\n3'-UUU...GGCAG________UAAA____AAAAA____________GCAGU...ACA-5'\n_____________\|_________________________________\|\n_____________53________________________________21\n	213 -- 219	22 -- 28	-21.7034 kcal/mol	9.76868 kcal/mol	3.9903 kcal/mol	186 220@LB@ \| \|@LB@5'-CGC...UAACG UAAAC GUCCCG UGAUC...GUC-3'@LB@ AAAGAGGA CGU UAUUAUUAUGC@LB@ \|\|\|\|\|\|\|\| \|:\| \|\|\|\|\|\|\|\|\|\|\|@LB@ UUUCUCCU GUA AUAAUAAUACG@LB@3'-UUU...GGCAG UAAA AAAAA GCAGU...ACA-5'@LB@ \| \|@LB@ 53 21@LB@@LB@@LB@Energy -7.94 kcal/mol@LB@Hybridization Energy -21.7034 kcal/mol@LB@Unfolding Energy - mRNA 9.76868 kcal/mol@LB@Unfolding Energy - sRNA 3.9903 kcal/mol@LB@@LB@Position - mRNA 187 -- 219@LB@Position - sRNA 22 -- 52@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 22 -- 28@LB@
156	0.03128	0.6177	b4512	ybdD	-7.87	0.110479	26 -- 38	43 -- 55	DUF466 family protein	_____________25____________39\n_____________\|_____________\|\n5'-GCA...UCAGA__G__________GUUGG...GGG-3'\n______________GU_AGGGGGGGAU\n______________\|\|_\|::\|:\|\|:\|\|\n______________CA_UUUCUCCUUA\n3'-UUU...CCGGG__G__________AAGUA...ACA-5'\n_____________\|_____________\|\n_____________56____________42\n	32 -- 38	43 -- 49	-14.3671 kcal/mol	5.55181 kcal/mol	0.946481 kcal/mol	25 39@LB@ \| \|@LB@5'-GCA...UCAGA G GUUGG...GGG-3'@LB@ GU AGGGGGGGAU@LB@ \|\| \|::\|:\|\|:\|\|@LB@ CA UUUCUCCUUA@LB@3'-UUU...CCGGG G AAGUA...ACA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -7.87 kcal/mol@LB@Hybridization Energy -14.3671 kcal/mol@LB@Unfolding Energy - mRNA 5.55181 kcal/mol@LB@Unfolding Energy - sRNA 0.946481 kcal/mol@LB@@LB@Position - mRNA 26 -- 38@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 32 -- 38@LB@Position Seed - sRNA 43 -- 49@LB@
143	0.02773	0.6177	b0409	secF	-7.84	0.111773	181 -- 195	43 -- 55	SecYEG protein translocase auxillary subunit	_____________180_____________196\n_____________\|_______________\|\n5'-CUU...AAGCU_____UCU_______GCGAU...UGU-3'\n______________GUCAA___GAGGAGU\n______________\|\|\|\|\|___\|\|\|\|\|:\|\n______________CAGUU___CUCCUUA\n3'-UUU...CCGGG_____U_________AAGUA...ACA-5'\n_____________\|_______________\|\n_____________56______________42\n	189 -- 195	43 -- 49	-13.7308 kcal/mol	4.94116 kcal/mol	0.946481 kcal/mol	180 196@LB@ \| \|@LB@5'-CUU...AAGCU UCU GCGAU...UGU-3'@LB@ GUCAA GAGGAGU@LB@ \|\|\|\|\| \|\|\|\|\|:\|@LB@ CAGUU CUCCUUA@LB@3'-UUU...CCGGG U AAGUA...ACA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -13.7308 kcal/mol@LB@Unfolding Energy - mRNA 4.94116 kcal/mol@LB@Unfolding Energy - sRNA 0.946481 kcal/mol@LB@@LB@Position - mRNA 181 -- 195@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 43 -- 49@LB@
103	0.02062	0.6177	b1332	ynaJ	-7.81	0.113347	36 -- 54	40 -- 55	DUF2534 family putative inner membrane protein	_____________35__________________55\n_____________\|___________________\|\n5'-AAA...ACUGA____GCC____A_______CCCGC...CCA-3'\n______________GUCA___GAGA_GAAUUUC\n______________\|\|\|\|___:\|\|\|_\|\|\|\|\|\|\|\n______________CAGU___UUCU_CUUAAAG\n3'-UUU...CCGGG___________C_______UAAAA...ACA-5'\n_____________\|___________________\|\n_____________56__________________39\n	48 -- 54	40 -- 46	-13.2524 kcal/mol	4.4222 kcal/mol	1.01784 kcal/mol	35 55@LB@ \| \|@LB@5'-AAA...ACUGA GCC A CCCGC...CCA-3'@LB@ GUCA GAGA GAAUUUC@LB@ \|\|\|\| :\|\|\| \|\|\|\|\|\|\|@LB@ CAGU UUCU CUUAAAG@LB@3'-UUU...CCGGG C UAAAA...ACA-5'@LB@ \| \|@LB@ 56 39@LB@@LB@@LB@Energy -7.81 kcal/mol@LB@Hybridization Energy -13.2524 kcal/mol@LB@Unfolding Energy - mRNA 4.4222 kcal/mol@LB@Unfolding Energy - sRNA 1.01784 kcal/mol@LB@@LB@Position - mRNA 36 -- 54@LB@Position - sRNA 40 -- 55@LB@Position Seed - mRNA 48 -- 54@LB@Position Seed - sRNA 40 -- 46@LB@
179	0.03684	0.6177	b0335	prpE	-7.81	0.113441	203 -- 221	38 -- 55	propionate--CoA ligase	_____________202_________________222\n_____________\|___________________\|\n5'-GCC...AUUAU___UUUU__C_________CAGCG...AAA-3'\n______________GUC____AG_GAAUUUUAU\n______________\|\|\|____\|\|_\|\|\|\|\|\|:\|\|\n______________CAG____UC_CUUAAAGUA\n3'-UUU...CCGGG___UUUC____________AAAAA...ACA-5'\n_____________\|___________________\|\n_____________56__________________37\n	215 -- 221	38 -- 44	-10.8576 kcal/mol	1.87676 kcal/mol	1.17036 kcal/mol	202 222@LB@ \| \|@LB@5'-GCC...AUUAU UUUU C CAGCG...AAA-3'@LB@ GUC AG GAAUUUUAU@LB@ \|\|\| \|\| \|\|\|\|\|\|:\|\|@LB@ CAG UC CUUAAAGUA@LB@3'-UUU...CCGGG UUUC AAAAA...ACA-5'@LB@ \| \|@LB@ 56 37@LB@@LB@@LB@Energy -7.81 kcal/mol@LB@Hybridization Energy -10.8576 kcal/mol@LB@Unfolding Energy - mRNA 1.87676 kcal/mol@LB@Unfolding Energy - sRNA 1.17036 kcal/mol@LB@@LB@Position - mRNA 203 -- 221@LB@Position - sRNA 38 -- 55@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 38 -- 44@LB@
59	0.01284	0.6177	b3453	ugpB	-7.80	0.113752	247 -- 260	43 -- 56	sn-glycerol-3-phosphate ABC transporter periplasmic binding protein	_____________246____________261\n_____________\|______________\|\n5'-UUC...ACUGG______U_______GCACA...UGG-3'\n______________CGUUAA_GGGGAAU\n______________\|\|\|:\|\|_\|:\|\|\|\|\|\n______________GCAGUU_CUCCUUA\n3'-UUU...ACCGG______U_______AAGUA...ACA-5'\n_____________\|______________\|\n_____________57_____________42\n	254 -- 260	43 -- 49	-14.4117 kcal/mol	5.65325 kcal/mol	0.953958 kcal/mol	246 261@LB@ \| \|@LB@5'-UUC...ACUGG U GCACA...UGG-3'@LB@ CGUUAA GGGGAAU@LB@ \|\|\|:\|\| \|:\|\|\|\|\|@LB@ GCAGUU CUCCUUA@LB@3'-UUU...ACCGG U AAGUA...ACA-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -7.80 kcal/mol@LB@Hybridization Energy -14.4117 kcal/mol@LB@Unfolding Energy - mRNA 5.65325 kcal/mol@LB@Unfolding Energy - sRNA 0.953958 kcal/mol@LB@@LB@Position - mRNA 247 -- 260@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 43 -- 49@LB@
187	0.03882	0.6177	b0403	malZ	-7.79	0.114240	189 -- 197	42 -- 50	maltodextrin glucosidase	_____________188_______198\n_____________\|_________\|\n5'-UUU...AUCAC_________AUGAU...AGC-3'\n______________AGGGGAAUU\n______________\|\|:\|\|\|\|\|\|\n______________UCUCCUUAA\n3'-UUU...CAGUU_________AGUAA...ACA-5'\n_____________\|_________\|\n_____________51________41\n	191 -- 197	42 -- 48	-10.7884 kcal/mol	2.13721 kcal/mol	0.856185 kcal/mol	188 198@LB@ \| \|@LB@5'-UUU...AUCAC AUGAU...AGC-3'@LB@ AGGGGAAUU@LB@ \|\|:\|\|\|\|\|\|@LB@ UCUCCUUAA@LB@3'-UUU...CAGUU AGUAA...ACA-5'@LB@ \| \|@LB@ 51 41@LB@@LB@@LB@Energy -7.79 kcal/mol@LB@Hybridization Energy -10.7884 kcal/mol@LB@Unfolding Energy - mRNA 2.13721 kcal/mol@LB@Unfolding Energy - sRNA 0.856185 kcal/mol@LB@@LB@Position - mRNA 189 -- 197@LB@Position - sRNA 42 -- 50@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 42 -- 48@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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