Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
13	0.003065	0.593	b4529	ydbJ	-10.82	0.027981	161 -- 194	44 -- 78	DUF333 family putative lipoprotein	_____________160___________________________________195\n_____________\|_____________________________________\|\n5'-GCA...UAUUU________________________AA__UC_______GAAAA...GUU-3'\n______________AUGG_CGGUGUCGU__UUGGCUUG__GA__AGAGGAA\n______________\|\|\|\|_\|::\|:\|\|\|:__\|\|\|\|\|::\|__:\|__\|\|\|\|\|\|\|\n______________UACC_GUUAUAGCG__AACCGGGC__UU__UCUCCUU\n_____3'-UUUUUU____G_________AU________AG___________AAAGU...ACA-5'\n_____________\|_____________________________________\|\n_____________79____________________________________43\n	188 -- 194	44 -- 50	-34.9495 kcal/mol	10.5981 kcal/mol	13.5333 kcal/mol	160 195@LB@ \| \|@LB@5'-GCA...UAUUU AA UC GAAAA...GUU-3'@LB@ AUGG CGGUGUCGU UUGGCUUG GA AGAGGAA@LB@ \|\|\|\| \|::\|:\|\|\|: \|\|\|\|\|::\| :\| \|\|\|\|\|\|\|@LB@ UACC GUUAUAGCG AACCGGGC UU UCUCCUU@LB@ 3'-UUUUUU G AU AG AAAGU...ACA-5'@LB@ \| \|@LB@ 79 43@LB@@LB@@LB@Energy -10.82 kcal/mol@LB@Hybridization Energy -34.9495 kcal/mol@LB@Unfolding Energy - mRNA 10.5981 kcal/mol@LB@Unfolding Energy - sRNA 13.5333 kcal/mol@LB@@LB@Position - mRNA 161 -- 194@LB@Position - sRNA 44 -- 78@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
94	0.01884	0.6177	b2048	cpsG	-10.80	0.028164	47 -- 56	45 -- 54	phosphomannomutase	_____________46_________57\n_____________\|__________\|\n5'-GGG...UUAUC__________UGAUG...GCG-3'\n______________UCGAAGAGGA\n______________\|\|:\|\|\|\|\|\|\|\n______________AGUUUCUCCU\n3'-UUU...CGGGC__________UAAAG...ACA-5'\n_____________\|__________\|\n_____________55_________44\n	50 -- 56	45 -- 51	-14.144 kcal/mol	2.41054 kcal/mol	0.929144 kcal/mol	46 57@LB@ \| \|@LB@5'-GGG...UUAUC UGAUG...GCG-3'@LB@ UCGAAGAGGA@LB@ \|\|:\|\|\|\|\|\|\|@LB@ AGUUUCUCCU@LB@3'-UUU...CGGGC UAAAG...ACA-5'@LB@ \| \|@LB@ 55 44@LB@@LB@@LB@Energy -10.80 kcal/mol@LB@Hybridization Energy -14.144 kcal/mol@LB@Unfolding Energy - mRNA 2.41054 kcal/mol@LB@Unfolding Energy - sRNA 0.929144 kcal/mol@LB@@LB@Position - mRNA 47 -- 56@LB@Position - sRNA 45 -- 54@LB@Position Seed - mRNA 50 -- 56@LB@Position Seed - sRNA 45 -- 51@LB@
52	0.01005	0.6177	b3985	rplJ	-10.76	0.028819	17 -- 26	48 -- 57	50S ribosomal subunit protein L10	_____________16_________27\n_____________\|__________\|\n5'-UGG...GGCCU__________CCGCA...UAA-3'\n______________CCGUCGAAGA\n______________\|\|\|\|\|:\|\|\|\|\n______________GGCAGUUUCU\n3'-UUU...AACCG__________CCUUA...ACA-5'\n_____________\|__________\|\n_____________58_________47\n	20 -- 26	48 -- 54	-14.4896 kcal/mol	0.999171 kcal/mol	2.73483 kcal/mol	16 27@LB@ \| \|@LB@5'-UGG...GGCCU CCGCA...UAA-3'@LB@ CCGUCGAAGA@LB@ \|\|\|\|\|:\|\|\|\|@LB@ GGCAGUUUCU@LB@3'-UUU...AACCG CCUUA...ACA-5'@LB@ \| \|@LB@ 58 47@LB@@LB@@LB@Energy -10.76 kcal/mol@LB@Hybridization Energy -14.4896 kcal/mol@LB@Unfolding Energy - mRNA 0.999171 kcal/mol@LB@Unfolding Energy - sRNA 2.73483 kcal/mol@LB@@LB@Position - mRNA 17 -- 26@LB@Position - sRNA 48 -- 57@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 48 -- 54@LB@
98	0.0193	0.6177	b2705	srlD	-10.75	0.028892	101 -- 114	40 -- 53	sorbitol-6-phosphate dehydrogenase	_____________100____________115\n_____________\|______________\|\n5'-CAC...UUAAA_____C________CGGGC...AUA-3'\n______________CGAAG_GGAAUUUC\n______________\|:\|\|\|_\|\|\|\|\|\|\|\|\n______________GUUUC_CCUUAAAG\n3'-UUU...GGGCA_____U________UAAAA...ACA-5'\n_____________\|______________\|\n_____________54_____________39\n	108 -- 114	40 -- 46	-15.1517 kcal/mol	3.39572 kcal/mol	1.00568 kcal/mol	100 115@LB@ \| \|@LB@5'-CAC...UUAAA C CGGGC...AUA-3'@LB@ CGAAG GGAAUUUC@LB@ \|:\|\|\| \|\|\|\|\|\|\|\|@LB@ GUUUC CCUUAAAG@LB@3'-UUU...GGGCA U UAAAA...ACA-5'@LB@ \| \|@LB@ 54 39@LB@@LB@@LB@Energy -10.75 kcal/mol@LB@Hybridization Energy -15.1517 kcal/mol@LB@Unfolding Energy - mRNA 3.39572 kcal/mol@LB@Unfolding Energy - sRNA 1.00568 kcal/mol@LB@@LB@Position - mRNA 101 -- 114@LB@Position - sRNA 40 -- 53@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 40 -- 46@LB@
11	0.002274	0.593	b1476	fdnI	-10.73	0.029150	152 -- 175	44 -- 69	formate dehydrogenase-N cytochrome B556 (gamma) subunit nitrate-inducible	_____________151________________________176\n_____________\|__________________________\|\n5'-CGU...ACUAC____G______________UA_____GUGGA...UUU-3'\n______________AUCG_UAUUGGCCCG__AA__AGGAA\n______________\|\|\|\|_\|\|\|\|\|\|\|\|\|\|__\|\|__\|\|\|\|\|\n______________UAGC_AUAACCGGGC__UU__UCCUU\n3'-UUU...GGUUA____G__________AG__UC_____AAAGU...ACA-5'\n_____________\|__________________________\|\n_____________70_________________________43\n	160 -- 166	55 -- 61	-29.2423 kcal/mol	7.20098 kcal/mol	11.3099 kcal/mol	151 176@LB@ \| \|@LB@5'-CGU...ACUAC G UA GUGGA...UUU-3'@LB@ AUCG UAUUGGCCCG AA AGGAA@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ UAGC AUAACCGGGC UU UCCUU@LB@3'-UUU...GGUUA G AG UC AAAGU...ACA-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -10.73 kcal/mol@LB@Hybridization Energy -29.2423 kcal/mol@LB@Unfolding Energy - mRNA 7.20098 kcal/mol@LB@Unfolding Energy - sRNA 11.3099 kcal/mol@LB@@LB@Position - mRNA 152 -- 175@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 55 -- 61@LB@
56	0.01199	0.6177	b0836	bssR	-10.68	0.029916	18 -- 32	42 -- 55	repressor of biofilm formation by indole transport regulation	_____________17______________33\n_____________\|_______________\|\n5'-AUU...GAAAG_______C_______CUACA...ACA-3'\n______________GUCAGGG_AGGGAUU\n______________\|\|\|\|::\|_\|\|\|:\|\|\|\n______________CAGUUUC_UCCUUAA\n3'-UUU...CCGGG_______________AGUAA...ACA-5'\n_____________\|_______________\|\n_____________56______________41\n	26 -- 32	42 -- 48	-16.3055 kcal/mol	4.68166 kcal/mol	0.947316 kcal/mol	17 33@LB@ \| \|@LB@5'-AUU...GAAAG C CUACA...ACA-3'@LB@ GUCAGGG AGGGAUU@LB@ \|\|\|\|::\| \|\|\|:\|\|\|@LB@ CAGUUUC UCCUUAA@LB@3'-UUU...CCGGG AGUAA...ACA-5'@LB@ \| \|@LB@ 56 41@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -16.3055 kcal/mol@LB@Unfolding Energy - mRNA 4.68166 kcal/mol@LB@Unfolding Energy - sRNA 0.947316 kcal/mol@LB@@LB@Position - mRNA 18 -- 32@LB@Position - sRNA 42 -- 55@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 42 -- 48@LB@
102	0.02032	0.6177	b1267	yciO	-10.56	0.031637	188 -- 197	44 -- 53	putative RNA binding protein	_____________187________198\n_____________\|__________\|\n5'-GCG...CACCA__________AUUAU...CAA-3'\n______________CAGAGAGGGA\n______________\|\|:\|\|\|\|\|:\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	189 -- 195	46 -- 52	-13.8947 kcal/mol	2.40877 kcal/mol	0.927997 kcal/mol	187 198@LB@ \| \|@LB@5'-GCG...CACCA AUUAU...CAA-3'@LB@ CAGAGAGGGA@LB@ \|\|:\|\|\|\|\|:\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -13.8947 kcal/mol@LB@Unfolding Energy - mRNA 2.40877 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 188 -- 197@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 46 -- 52@LB@
20	0.00399	0.5992	b4175	hflC	-10.54	0.031960	184 -- 196	43 -- 56	HflB protease modulator specific for phage lambda cII repressor	_____________183____________197\n_____________\|______________\|\n5'-UUA...ACCAG______________AACGA...GUU-3'\n______________CGUCAGGG_GGAAU\n______________\|\|\|\|\|::\|_\|\|\|\|\|\n______________GCAGUUUC_CCUUA\n3'-UUU...ACCGG________U_____AAGUA...ACA-5'\n_____________\|______________\|\n_____________57_____________42\n	185 -- 191	49 -- 55	-15.8123 kcal/mol	4.32193 kcal/mol	0.953958 kcal/mol	183 197@LB@ \| \|@LB@5'-UUA...ACCAG AACGA...GUU-3'@LB@ CGUCAGGG GGAAU@LB@ \|\|\|\|\|::\| \|\|\|\|\|@LB@ GCAGUUUC CCUUA@LB@3'-UUU...ACCGG U AAGUA...ACA-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -15.8123 kcal/mol@LB@Unfolding Energy - mRNA 4.32193 kcal/mol@LB@Unfolding Energy - sRNA 0.953958 kcal/mol@LB@@LB@Position - mRNA 184 -- 196@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 49 -- 55@LB@
27	0.005027	0.5992	b3745	viaA	-10.46	0.033099	243 -- 253	42 -- 52	stimulator of RavA ATPase activity; von Willebrand factor domain protein	_____________242_________254\n_____________\|___________\|\n5'-ACG...UCAGC___________GAUCG...CGG-3'\n______________GAAGAGGGAUU\n______________:\|\|\|\|\|\|:\|\|\|\n______________UUUCUCCUUAA\n3'-UUU...GGCAG___________AGUAA...ACA-5'\n_____________\|___________\|\n_____________53__________41\n	247 -- 253	42 -- 48	-12.7293 kcal/mol	1.40033 kcal/mol	0.866923 kcal/mol	242 254@LB@ \| \|@LB@5'-ACG...UCAGC GAUCG...CGG-3'@LB@ GAAGAGGGAUU@LB@ :\|\|\|\|\|\|:\|\|\|@LB@ UUUCUCCUUAA@LB@3'-UUU...GGCAG AGUAA...ACA-5'@LB@ \| \|@LB@ 53 41@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -12.7293 kcal/mol@LB@Unfolding Energy - mRNA 1.40033 kcal/mol@LB@Unfolding Energy - sRNA 0.866923 kcal/mol@LB@@LB@Position - mRNA 243 -- 253@LB@Position - sRNA 42 -- 52@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 42 -- 48@LB@
72	0.015	0.6177	b2513	yfgM	-10.40	0.034106	179 -- 190	45 -- 55	ancillary SecYEG translocon subunit; putative anti-RcsB factor	_____________178__________191\n_____________\|____________\|\n5'-GGC...ACUGG________A___GAAGG...UUG-3'\n______________GUUAAGGA_GGA\n______________\|\|:\|\|:\|\|_\|\|\|\n______________CAGUUUCU_CCU\n3'-UUU...CCGGG____________UAAAG...ACA-5'\n_____________\|____________\|\n_____________56___________44\n	180 -- 186	48 -- 54	-12.2699 kcal/mol	0.940443 kcal/mol	0.930875 kcal/mol	178 191@LB@ \| \|@LB@5'-GGC...ACUGG A GAAGG...UUG-3'@LB@ GUUAAGGA GGA@LB@ \|\|:\|\|:\|\| \|\|\|@LB@ CAGUUUCU CCU@LB@3'-UUU...CCGGG UAAAG...ACA-5'@LB@ \| \|@LB@ 56 44@LB@@LB@@LB@Energy -10.40 kcal/mol@LB@Hybridization Energy -12.2699 kcal/mol@LB@Unfolding Energy - mRNA 0.940443 kcal/mol@LB@Unfolding Energy - sRNA 0.930875 kcal/mol@LB@@LB@Position - mRNA 179 -- 190@LB@Position - sRNA 45 -- 55@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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