Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
69	0.003755	0.191	b0889	lrp	-14.02	0.046951	165 -- 212	1 -- 32	leucine-responsive global transcriptional regulator		_____________164______________________________________________213\n_____________\|________________________________________________\|\n5'-GUG...GUCAG______GUA____AGGAAUA________CAAUAAUAAU_____AUA__AAGAA...AGC-3'\n______________ACAGGA___GGGA_______CAGAGAGA__________GGUAG___GC\n______________\|\|\|::\|___\|\|\|\|_______\|\|:\|\|\|:\|__________:\|:\|\|___\|\|\n______________UGUUUU___CCCU_______GUUUCUUU__________UCGUC___CG\n3'-UUU...CGAGG______AC_____GUA___________________________AC___-5'\n_____________\|_______________________________________________\|\n_____________33______________________________________________1\n	186 -- 192	10 -- 16	-19.2298 kcal/mol	2.66644 kcal/mol	2.53929 kcal/mol	164 213@LB@ \| \|@LB@5'-GUG...GUCAG GUA AGGAAUA CAAUAAUAAU AUA AAGAA...AGC-3'@LB@ ACAGGA GGGA CAGAGAGA GGUAG GC@LB@ \|\|\|::\| \|\|\|\| \|\|:\|\|\|:\| :\|:\|\| \|\|@LB@ UGUUUU CCCU GUUUCUUU UCGUC CG@LB@3'-UUU...CGAGG AC GUA AC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -14.02 kcal/mol@LB@Hybridization Energy -19.2298 kcal/mol@LB@Unfolding Energy - mRNA 2.66644 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 165 -- 212@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 10 -- 16@LB@
151	0.01116	0.2504	b0903	pflB	-10.70	0.211701	140 -- 166	4 -- 34	formate C-acetyltransferase 1 anaerobic; pyruvate formate-lyase 1		_____________139_____________________________167\n_____________\|_______________________________\|\n5'-CAC...GACCA____U______U__________UAC______AAAUA...ACU-3'\n______________CCAU__AAUGG___UGUCGAAG____GCAGU\n______________\|\|\|:__\|\|\|\|\|___::\|\|:\|\|\|____\|\|\|\|\|\n______________GGUG__UUACC___GUAGUUUC____CGUCA\n3'-UUU...CCCGA____UU_____CCU________UUUU_____CCG-5'\n_____________\|_______________________________\|\n_____________35______________________________3\n	152 -- 158	13 -- 19	-21.5315 kcal/mol	6.56187 kcal/mol	4.26884 kcal/mol	139 167@LB@ \| \|@LB@5'-CAC...GACCA U U UAC AAAUA...ACU-3'@LB@ CCAU AAUGG UGUCGAAG GCAGU@LB@ \|\|\|: \|\|\|\|\| ::\|\|:\|\|\| \|\|\|\|\|@LB@ GGUG UUACC GUAGUUUC CGUCA@LB@3'-UUU...CCCGA UU CCU UUUU CCG-5'@LB@ \| \|@LB@ 35 3@LB@@LB@@LB@Energy -10.70 kcal/mol@LB@Hybridization Energy -21.5315 kcal/mol@LB@Unfolding Energy - mRNA 6.56187 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 140 -- 166@LB@Position - sRNA 4 -- 34@LB@Position Seed - mRNA 152 -- 158@LB@Position Seed - sRNA 13 -- 19@LB@
189	0.01393	0.2529	b0934	ssuC	-14.25	0.041816	137 -- 153	1 -- 17	aliphatic sulfonate ABC transporter permease		_____________136_______________154\n_____________\|_________________\|\n5'-UGC...AUCCG_____C___________GAAUG...GGC-3'\n______________CAAGG_GAAGCGGUGGC\n______________\|\|\|:\|_:\|\|\|\|:\|\|\|\|\|\n______________GUUUC_UUUCGUCACCG\n3'-UUU...CUGUA_____U___________-5'\n_____________\|________________\|\n_____________18_______________1\n	147 -- 153	1 -- 7	-24.8998 kcal/mol	9.21593 kcal/mol	1.43853 kcal/mol	136 154@LB@ \| \|@LB@5'-UGC...AUCCG C GAAUG...GGC-3'@LB@ CAAGG GAAGCGGUGGC@LB@ \|\|\|:\| :\|\|\|\|:\|\|\|\|\|@LB@ GUUUC UUUCGUCACCG@LB@3'-UUU...CUGUA U -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -24.8998 kcal/mol@LB@Unfolding Energy - mRNA 9.21593 kcal/mol@LB@Unfolding Energy - sRNA 1.43853 kcal/mol@LB@@LB@Position - mRNA 137 -- 153@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 1 -- 7@LB@
194	0.01408	0.2529	b0952	pqiC	-11.52	0.151794	181 -- 195	48 -- 61	DUF330 family putative lipoprotein		_____________180_____________196\n_____________\|_______________\|\n5'-ACG...AUCCA_________AG____AAACA...GCG-3'\n______________GAGCCGAAG__GGCG\n______________\|\|:\|\|\|\|\|:__\|\|\|\|\n______________CUUGGCUUU__CCGC\n3'-UUU...UGGAA_________A_____CCCAA...CCG-5'\n_____________\|_______________\|\n_____________62______________47\n	183 -- 189	53 -- 59	-21.1549 kcal/mol	5.66908 kcal/mol	3.96136 kcal/mol	180 196@LB@ \| \|@LB@5'-ACG...AUCCA AG AAACA...GCG-3'@LB@ GAGCCGAAG GGCG@LB@ \|\|:\|\|\|\|\|: \|\|\|\|@LB@ CUUGGCUUU CCGC@LB@3'-UUU...UGGAA A CCCAA...CCG-5'@LB@ \| \|@LB@ 62 47@LB@@LB@@LB@Energy -11.52 kcal/mol@LB@Hybridization Energy -21.1549 kcal/mol@LB@Unfolding Energy - mRNA 5.66908 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 181 -- 195@LB@Position - sRNA 48 -- 61@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 53 -- 59@LB@
137	0.0102	0.2504	b0957	ompA	-10.25	0.250752	198 -- 232	1 -- 30	outer membrane protein A (3a;II*;G;d)		_____________197_________________________________233\n_____________\|___________________________________\|\n5'-CUU...CGCAA______AAAAA____GCUAUCGC__UU________ACUGG...AAC-3'\n______________AAAAUG_____GACA________GA__GCAGUGGC\n______________\|\|\|\|\|\|_____\|\|\|\|________:\|__\|\|\|\|\|\|\|\|\n______________UUUUAC_____CUGU________UU__CGUCACCG\n3'-UUU...AGGUG______CC_______AGUUUC____UU________-5'\n_____________\|__________________________________\|\n_____________31_________________________________1\n	226 -- 232	1 -- 7	-21.624 kcal/mol	8.85247 kcal/mol	2.51911 kcal/mol	197 233@LB@ \| \|@LB@5'-CUU...CGCAA AAAAA GCUAUCGC UU ACUGG...AAC-3'@LB@ AAAAUG GACA GA GCAGUGGC@LB@ \|\|\|\|\|\| \|\|\|\| :\| \|\|\|\|\|\|\|\|@LB@ UUUUAC CUGU UU CGUCACCG@LB@3'-UUU...AGGUG CC AGUUUC UU -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -21.624 kcal/mol@LB@Unfolding Energy - mRNA 8.85247 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 198 -- 232@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 1 -- 7@LB@
42	0.002133	0.1648	b0968	yccX	-12.44	0.101807	184 -- 198	5 -- 23	weak acylphosphatase		_____________183_________________199\n_____________\|___________________\|\n5'-GGC...AUUAA___________________CAAUG...CCG-3'\n______________GGAUA____AGGAGAGCAG\n______________\|\|\|:\|____\|\|:\|:\|\|\|\|\|\n______________CCUGU____UCUUUUCGUC\n3'-UUU...UUACC_____AGUU__________ACCG-5'\n_____________\|___________________\|\n_____________24__________________4\n	192 -- 198	5 -- 11	-17.1825 kcal/mol	3.17176 kcal/mol	1.57523 kcal/mol	183 199@LB@ \| \|@LB@5'-GGC...AUUAA CAAUG...CCG-3'@LB@ GGAUA AGGAGAGCAG@LB@ \|\|\|:\| \|\|:\|:\|\|\|\|\|@LB@ CCUGU UCUUUUCGUC@LB@3'-UUU...UUACC AGUU ACCG-5'@LB@ \| \|@LB@ 24 4@LB@@LB@@LB@Energy -12.44 kcal/mol@LB@Hybridization Energy -17.1825 kcal/mol@LB@Unfolding Energy - mRNA 3.17176 kcal/mol@LB@Unfolding Energy - sRNA 1.57523 kcal/mol@LB@@LB@Position - mRNA 184 -- 198@LB@Position - sRNA 5 -- 23@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 5 -- 11@LB@
41	0.002071	0.1648	b0992	yccM	-9.88	0.287125	161 -- 167	1 -- 7	putative 4Fe-4S membrane protein		_____________160_____168\n_____________\|_______\|\n5'-AUC...UAGAA_______AUUUG...AAG-3'\n______________UAGUGGC\n______________:\|\|\|\|\|\|\n______________GUCACCG\n3'-UUU...UUUUC_______-5'\n_____________\|______\|\n_____________8______1\n	161 -- 167	1 -- 7	-11.7646 kcal/mol	0.660682 kcal/mol	1.22779 kcal/mol	160 168@LB@ \| \|@LB@5'-AUC...UAGAA AUUUG...AAG-3'@LB@ UAGUGGC@LB@ :\|\|\|\|\|\|@LB@ GUCACCG@LB@3'-UUU...UUUUC -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -11.7646 kcal/mol@LB@Unfolding Energy - mRNA 0.660682 kcal/mol@LB@Unfolding Energy - sRNA 1.22779 kcal/mol@LB@@LB@Position - mRNA 161 -- 167@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 1 -- 7@LB@
143	0.01049	0.2504	b1004	wrbA	-9.61	0.314473	272 -- 293	1 -- 25	NAD(P)H:quinone oxidoreductase		_____________271________________________294\n_____________\|__________________________\|\n5'-UUA...GCUGA___U_________C_______A____GCUGAAG-3'\n______________GGG_GCA____AG_AAAGUGG_UGGC\n______________\|\|\|_:\|\|____\|\|_\|\|\|\|::\|_\|\|\|\|\n______________CCC_UGU____UC_UUUCGUC_ACCG\n3'-UUU...UUUUA___C___AGUU__U____________-5'\n_____________\|_________________________\|\n_____________26________________________1\n	282 -- 288	5 -- 11	-20.1219 kcal/mol	7.99134 kcal/mol	2.51748 kcal/mol	271 294@LB@ \| \|@LB@5'-UUA...GCUGA U C A GCUGAAG-3'@LB@ GGG GCA AG AAAGUGG UGGC@LB@ \|\|\| :\|\| \|\| \|\|\|\|::\| \|\|\|\|@LB@ CCC UGU UC UUUCGUC ACCG@LB@3'-UUU...UUUUA C AGUU U -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -9.61 kcal/mol@LB@Hybridization Energy -20.1219 kcal/mol@LB@Unfolding Energy - mRNA 7.99134 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 272 -- 293@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 282 -- 288@LB@Position Seed - sRNA 5 -- 11@LB@
167	0.01216	0.2504	b1013	rutR	-12.90	0.082087	181 -- 218	3 -- 35	rut operon transcriptional repressor for	b0796	_____________180________________________________________219\n_____________\|__________________________________________\|\n5'-CAA...GCCAA_______________GAGCGCAUGACG_____C_________AAAAC...AAU-3'\n______________UCUACAAGA_GGGGA____________CAAGG____GCAGUG\n______________\|\|:\|\|\|\|:\|_\|\|\|\|\|____________\|\|\|:\|____\|\|\|\|\|\|\n______________AGGUGUUUU_CCCCU____________GUUUC____CGUCAC\n3'-UUU...ACCCG_________A_____GUA______________UUUU______CG-5'\n_____________\|__________________________________________\|\n_____________36_________________________________________2\n	181 -- 187	29 -- 35	-29.1146 kcal/mol	11.1798 kcal/mol	5.03644 kcal/mol	180 219@LB@ \| \|@LB@5'-CAA...GCCAA GAGCGCAUGACG C AAAAC...AAU-3'@LB@ UCUACAAGA GGGGA CAAGG GCAGUG@LB@ \|\|:\|\|\|\|:\| \|\|\|\|\| \|\|\|:\| \|\|\|\|\|\|@LB@ AGGUGUUUU CCCCU GUUUC CGUCAC@LB@3'-UUU...ACCCG A GUA UUUU CG-5'@LB@ \| \|@LB@ 36 2@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -29.1146 kcal/mol@LB@Unfolding Energy - mRNA 11.1798 kcal/mol@LB@Unfolding Energy - sRNA 5.03644 kcal/mol@LB@@LB@Position - mRNA 181 -- 218@LB@Position - sRNA 3 -- 35@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 29 -- 35@LB@
10	0.0003274	0.1087	b1020	phoH	-9.93	0.281763	74 -- 85	48 -- 59	ATP-binding protein; putative PhoH family P-loop ATPase		_____________73___________86\n_____________\|____________\|\n5'-AAU...AUGUC____________UUUUA...AAC-3'\n______________ACUGAAAUGGUG\n______________\|\|:\|\|\|\|\|\|\|:\|\n______________UGGCUUUACCGC\n3'-UUU...GAACU____________CCCAA...CCG-5'\n_____________\|____________\|\n_____________60___________47\n	79 -- 85	48 -- 54	-17.134 kcal/mol	3.24305 kcal/mol	3.96136 kcal/mol	73 86@LB@ \| \|@LB@5'-AAU...AUGUC UUUUA...AAC-3'@LB@ ACUGAAAUGGUG@LB@ \|\|:\|\|\|\|\|\|\|:\|@LB@ UGGCUUUACCGC@LB@3'-UUU...GAACU CCCAA...CCG-5'@LB@ \| \|@LB@ 60 47@LB@@LB@@LB@Energy -9.93 kcal/mol@LB@Hybridization Energy -17.134 kcal/mol@LB@Unfolding Energy - mRNA 3.24305 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 74 -- 85@LB@Position - sRNA 48 -- 59@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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