Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
109	0.007372	0.2348	b3701	dnaN	-13.44	0.063163	149 -- 162	9 -- 22	DNA polymerase III beta subunit	_____________148____________163\n_____________\|______________\|\n5'-GGC...GCCAC______________UUUUU...UGC-3'\n______________GAUAUCAAAGAAGA\n______________\|\|:\|\|\|\|\|\|\|\|\|:\|\n______________CUGUAGUUUCUUUU\n3'-UUU...UACCC______________CGUCACCG-5'\n_____________\|______________\|\n_____________23_____________8\n	156 -- 162	9 -- 15	-16.0152 kcal/mol	2.0875 kcal/mol	0.488786 kcal/mol	148 163@LB@ \| \|@LB@5'-GGC...GCCAC UUUUU...UGC-3'@LB@ GAUAUCAAAGAAGA@LB@ \|\|:\|\|\|\|\|\|\|\|\|:\|@LB@ CUGUAGUUUCUUUU@LB@3'-UUU...UACCC CGUCACCG-5'@LB@ \| \|@LB@ 23 8@LB@@LB@@LB@Energy -13.44 kcal/mol@LB@Hybridization Energy -16.0152 kcal/mol@LB@Unfolding Energy - mRNA 2.0875 kcal/mol@LB@Unfolding Energy - sRNA 0.488786 kcal/mol@LB@@LB@Position - mRNA 149 -- 162@LB@Position - sRNA 9 -- 22@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 9 -- 15@LB@
26	0.001009	0.1361	b3731	atpC	-14.21	0.042680	144 -- 160	2 -- 20	F1 sector of membrane-bound ATP synthase epsilon subunit	_____________143_________________161\n_____________\|___________________\|\n5'-UCC...GGUUC______________U____AAAAA...UGG-3'\n______________CAUCGAAGA__AGC_GUGG\n______________\|\|\|\|:\|\|\|\|__\|\|\|_\|\|\|\|\n______________GUAGUUUCU__UCG_CACC\n3'-UUU...CCCCU_________UU___U____G-5'\n_____________\|___________________\|\n_____________21__________________1\n	146 -- 152	12 -- 18	-19.7705 kcal/mol	4.1055 kcal/mol	1.45839 kcal/mol	143 161@LB@ \| \|@LB@5'-UCC...GGUUC U AAAAA...UGG-3'@LB@ CAUCGAAGA AGC GUGG@LB@ \|\|\|\|:\|\|\|\| \|\|\| \|\|\|\|@LB@ GUAGUUUCU UCG CACC@LB@3'-UUU...CCCCU UU U G-5'@LB@ \| \|@LB@ 21 1@LB@@LB@@LB@Energy -14.21 kcal/mol@LB@Hybridization Energy -19.7705 kcal/mol@LB@Unfolding Energy - mRNA 4.1055 kcal/mol@LB@Unfolding Energy - sRNA 1.45839 kcal/mol@LB@@LB@Position - mRNA 144 -- 160@LB@Position - sRNA 2 -- 20@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 12 -- 18@LB@
25	0.0009834	0.1361	b3733	atpG	-13.27	0.068654	183 -- 205	1 -- 22	F1 sector of membrane-bound ATP synthase gamma subunit	_____________182_____________________206\n_____________\|_______________________\|\n5'-GGC...CGCAA________________UCA____CGGCG...GUA-3'\n______________GGCAUUGAGGAGAAGC___UGGC\n______________\|:\|\|\|::\|:\|\|:\|\|\|\|___\|\|\|\|\n______________CUGUAGUUUCUUUUCG___ACCG\n3'-UUU...UACCC________________UC_____-5'\n_____________\|______________________\|\n_____________23_____________________1\n	192 -- 198	7 -- 13	-25.5897 kcal/mol	10.7799 kcal/mol	1.54023 kcal/mol	182 206@LB@ \| \|@LB@5'-GGC...CGCAA UCA CGGCG...GUA-3'@LB@ GGCAUUGAGGAGAAGC UGGC@LB@ \|:\|\|\|::\|:\|\|:\|\|\|\| \|\|\|\|@LB@ CUGUAGUUUCUUUUCG ACCG@LB@3'-UUU...UACCC UC -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -13.27 kcal/mol@LB@Hybridization Energy -25.5897 kcal/mol@LB@Unfolding Energy - mRNA 10.7799 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 183 -- 205@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 7 -- 13@LB@
14	0.0006392	0.1243	b3745	viaA	-13.90	0.050132	246 -- 264	10 -- 25	stimulator of RavA ATPase activity; von Willebrand factor domain protein	_____________245_________________265\n_____________\|___________________\|\n5'-ACG...GCGAA_A_____UG__________UGAUC...CGG-3'\n______________G_GGGAU__AUCGAAGAGA\n______________\|_\|\|\|\|:__\|\|\|:\|\|\|\|:\|\n______________C_CCCUG__UAGUUUCUUU\n3'-UUU...UUUUA___________________UCGUC...CCG-5'\n_____________\|___________________\|\n_____________26__________________9\n	258 -- 264	10 -- 16	-16.9183 kcal/mol	2.35086 kcal/mol	0.670388 kcal/mol	245 265@LB@ \| \|@LB@5'-ACG...GCGAA A UG UGAUC...CGG-3'@LB@ G GGGAU AUCGAAGAGA@LB@ \| \|\|\|\|: \|\|\|:\|\|\|\|:\|@LB@ C CCCUG UAGUUUCUUU@LB@3'-UUU...UUUUA UCGUC...CCG-5'@LB@ \| \|@LB@ 26 9@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -16.9183 kcal/mol@LB@Unfolding Energy - mRNA 2.35086 kcal/mol@LB@Unfolding Energy - sRNA 0.670388 kcal/mol@LB@@LB@Position - mRNA 246 -- 264@LB@Position - sRNA 10 -- 25@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 10 -- 16@LB@
1	0	0	b3752	rbsK	-20.54	0.000534	137 -- 155	1 -- 21	ribokinase	_____________136___________________156\n_____________\|_____________________\|\n5'-GCA...GUGUA_____________________ACGCG...GUA-3'\n______________AUA_UAAAGAAAAGCAG_GGC\n______________\|:\|_:\|\|\|\|\|\|\|\|\|\|\|\|_\|\|\|\n______________UGU_GUUUCUUUUCGUC_CCG\n3'-UUU...ACCCC___A_____________A___-5'\n_____________\|____________________\|\n_____________22___________________1\n	146 -- 152	5 -- 11	-23.5677 kcal/mol	1.51892 kcal/mol	1.51103 kcal/mol	136 156@LB@ \| \|@LB@5'-GCA...GUGUA ACGCG...GUA-3'@LB@ AUA UAAAGAAAAGCAG GGC@LB@ \|:\| :\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|@LB@ UGU GUUUCUUUUCGUC CCG@LB@3'-UUU...ACCCC A A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -20.54 kcal/mol@LB@Hybridization Energy -23.5677 kcal/mol@LB@Unfolding Energy - mRNA 1.51892 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 137 -- 155@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 5 -- 11@LB@
102	0.006464	0.2224	b3785	wzzE	-10.51	0.228012	146 -- 168	1 -- 22	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein	_____________145_____________________169\n_____________\|_______________________\|\n5'-GCU...ACGCA___UG__U_____UC________AGCCC...UUA-3'\n______________GAC__CG_AGAAA__GUGGUGGC\n______________\|\|\|__\|:_\|\|\|\|\|__\|::\|\|\|\|\|\n______________CUG__GU_UCUUU__CGUCACCG\n3'-UUU...UACCC___UA__U_____U_________-5'\n_____________\|______________________\|\n_____________23_____________________1\n	162 -- 168	1 -- 7	-19.8866 kcal/mol	7.83936 kcal/mol	1.54023 kcal/mol	145 169@LB@ \| \|@LB@5'-GCU...ACGCA UG U UC AGCCC...UUA-3'@LB@ GAC CG AGAAA GUGGUGGC@LB@ \|\|\| \|: \|\|\|\|\| \|::\|\|\|\|\|@LB@ CUG GU UCUUU CGUCACCG@LB@3'-UUU...UACCC UA U U -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -10.51 kcal/mol@LB@Hybridization Energy -19.8866 kcal/mol@LB@Unfolding Energy - mRNA 7.83936 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 146 -- 168@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 1 -- 7@LB@
98	0.006107	0.2179	b3822	recQ	-9.35	0.343777	185 -- 205	1 -- 22	ATP-dependent DNA helicase	_____________184_____________________206\n_____________\|_______________________\|\n5'-CGC...UUUCA___A_______U___U_______GCAGG...UUA-3'\n______________GGC_AUCGGGG_GAA___GUGGC\n______________\|:\|_\|\|\|:::\|_:\|\|___\|\|\|\|\|\n______________CUG_UAGUUUC_UUU___CACCG\n3'-UUU...UACCC___________U___CGU_____-5'\n_____________\|______________________\|\n_____________23_____________________1\n	189 -- 195	13 -- 19	-17.8698 kcal/mol	6.98341 kcal/mol	1.54023 kcal/mol	184 206@LB@ \| \|@LB@5'-CGC...UUUCA A U U GCAGG...UUA-3'@LB@ GGC AUCGGGG GAA GUGGC@LB@ \|:\| \|\|\|:::\| :\|\| \|\|\|\|\|@LB@ CUG UAGUUUC UUU CACCG@LB@3'-UUU...UACCC U CGU -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -9.35 kcal/mol@LB@Hybridization Energy -17.8698 kcal/mol@LB@Unfolding Energy - mRNA 6.98341 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 185 -- 205@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 13 -- 19@LB@
157	0.0114	0.2504	b3833	ubiE	-14.57	0.035245	198 -- 236	1 -- 30	bifunctional 2-octaprenyl-6-methoxy-1 4-benzoquinone methylase/ S-adenosylmethionine:2-DMK methyltransferase	_____________197_____________________________________237\n_____________\|_______________________________________\|\n5'-ACA...GCAUU_______U____AA____C______CGAC___CUU____UUUCA...CAU-3'\n______________GAGAUGG_GGAU__GUCA_AAGAAA____GCA___UGGU\n______________:\|:\|\|\|\|_\|\|\|:__:\|\|\|_\|\|\|\|\|\|____\|\|\|___\|\|\|:\n______________UUUUACC_CCUG__UAGU_UUCUUU____CGU___ACCG\n3'-UUU...AGGUG_________________________U______C______-5'\n_____________\|______________________________________\|\n_____________31_____________________________________1\n	198 -- 204	24 -- 30	-23.6374 kcal/mol	6.55293 kcal/mol	2.51911 kcal/mol	197 237@LB@ \| \|@LB@5'-ACA...GCAUU U AA C CGAC CUU UUUCA...CAU-3'@LB@ GAGAUGG GGAU GUCA AAGAAA GCA UGGU@LB@ :\|:\|\|\|\| \|\|\|: :\|\|\| \|\|\|\|\|\| \|\|\| \|\|\|:@LB@ UUUUACC CCUG UAGU UUCUUU CGU ACCG@LB@3'-UUU...AGGUG U C -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -14.57 kcal/mol@LB@Hybridization Energy -23.6374 kcal/mol@LB@Unfolding Energy - mRNA 6.55293 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 198 -- 236@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 24 -- 30@LB@
73	0.004089	0.1966	b3857	mobA	-16.43	0.011914	256 -- 282	7 -- 29	molybdopterin-guanine dinucleotide synthase	_____________255_________________________283\n_____________\|___________________________\|\n5'-UCC...CAGAC_______C_____AGA_______UU__UUGAA...AAC-3'\n______________GAAUGGG_GGCGU___UAAAGGA__GC\n______________:\|\|\|\|\|\|_\|:\|:\|___:\|\|\|\|:\|__\|\|\n______________UUUACCC_CUGUA___GUUUCUU__CG\n3'-UUU...GGUGU_______________________UU__UCACCG-5'\n_____________\|___________________________\|\n_____________30__________________________6\n	272 -- 278	11 -- 17	-21.4093 kcal/mol	4.27411 kcal/mol	0.706428 kcal/mol	255 283@LB@ \| \|@LB@5'-UCC...CAGAC C AGA UU UUGAA...AAC-3'@LB@ GAAUGGG GGCGU UAAAGGA GC@LB@ :\|\|\|\|\|\| \|:\|:\| :\|\|\|\|:\| \|\|@LB@ UUUACCC CUGUA GUUUCUU CG@LB@3'-UUU...GGUGU UU UCACCG-5'@LB@ \| \|@LB@ 30 6@LB@@LB@@LB@Energy -16.43 kcal/mol@LB@Hybridization Energy -21.4093 kcal/mol@LB@Unfolding Energy - mRNA 4.27411 kcal/mol@LB@Unfolding Energy - sRNA 0.706428 kcal/mol@LB@@LB@Position - mRNA 256 -- 282@LB@Position - sRNA 7 -- 29@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 11 -- 17@LB@
159	0.01174	0.2504	b3886	yihY	-12.46	0.100688	17 -- 39	6 -- 26	BrkB family putative transporter inner membrane protein	_____________16______________________40\n_____________\|_______________________\|\n5'-UAU...AGAUC________CG____CCU______CGAAU...CAA-3'\n______________UGGGGAUG__CAAA___GAAGCA\n______________\|\|\|\|\|\|::__\|\|\|\|___:\|\|\|\|\|\n______________ACCCCUGU__GUUU___UUUCGU\n3'-UUU...GUUUU________A_____CU_______CACCG-5'\n_____________\|_______________________\|\n_____________27______________________5\n	18 -- 24	19 -- 25	-18.1196 kcal/mol	4.95346 kcal/mol	0.706421 kcal/mol	16 40@LB@ \| \|@LB@5'-UAU...AGAUC CG CCU CGAAU...CAA-3'@LB@ UGGGGAUG CAAA GAAGCA@LB@ \|\|\|\|\|\|:: \|\|\|\| :\|\|\|\|\|@LB@ ACCCCUGU GUUU UUUCGU@LB@3'-UUU...GUUUU A CU CACCG-5'@LB@ \| \|@LB@ 27 5@LB@@LB@@LB@Energy -12.46 kcal/mol@LB@Hybridization Energy -18.1196 kcal/mol@LB@Unfolding Energy - mRNA 4.95346 kcal/mol@LB@Unfolding Energy - sRNA 0.706421 kcal/mol@LB@@LB@Position - mRNA 17 -- 39@LB@Position - sRNA 6 -- 26@LB@Position Seed - mRNA 18 -- 24@LB@Position Seed - sRNA 19 -- 25@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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