| 41 |
0.001775 |
0.1444 |
bsu36710 |
fdhD |
-11.37 |
0.133163 |
186 -- 192 |
30 -- 36 |
protein FdhD |
|
_____________185_____193\n_____________|_______|\n5'-UCG...AUUGC_______AAGAG...CAG-3'\n______________GGAGGGG\n______________:||||||\n______________UCUCCCC\n3'-UUU...GACUA_______AACCC...AUA-5'\n_____________|_______|\n_____________37______29\n |
186 -- 192 |
30 -- 36 |
-13.1021 kcal/mol |
1.59974 kcal/mol |
0.133643 kcal/mol |
185 193@LB@ | |@LB@5'-UCG...AUUGC AAGAG...CAG-3'@LB@ GGAGGGG@LB@ :||||||@LB@ UCUCCCC@LB@3'-UUU...GACUA AACCC...AUA-5'@LB@ | |@LB@ 37 29@LB@@LB@@LB@Energy -11.37 kcal/mol@LB@Hybridization Energy -13.1021 kcal/mol@LB@Unfolding Energy - mRNA 1.59974 kcal/mol@LB@Unfolding Energy - sRNA 0.133643 kcal/mol@LB@@LB@Position - mRNA 186 -- 192@LB@Position - sRNA 30 -- 36@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 42 |
0.001862 |
0.1479 |
bsu40320 |
argI |
-8.56 |
0.330283 |
1 -- 17 |
32 -- 48 |
arginase |
|
______________1_________________18\n______________|_________________|\n___________5'-_____C_______CC___AGCAG...UGA-3'\n______________UCUUG_AUUUGAU___GG\n______________|||||_||:||||___||\n______________AGAAC_UAGACUA___CC\n3'-UUU...AGCCU_____________UCU__CCAAC...AUA-5'\n_____________|__________________|\n_____________49_________________31\n |
7 -- 13 |
37 -- 43 |
-12.1859 kcal/mol |
2.6521 kcal/mol |
0.970016 kcal/mol |
1 18@LB@ | |@LB@ 5'- C CC AGCAG...UGA-3'@LB@ UCUUG AUUUGAU GG@LB@ ||||| ||:|||| ||@LB@ AGAAC UAGACUA CC@LB@3'-UUU...AGCCU UCU CCAAC...AUA-5'@LB@ | |@LB@ 49 31@LB@@LB@@LB@Energy -8.56 kcal/mol@LB@Hybridization Energy -12.1859 kcal/mol@LB@Unfolding Energy - mRNA 2.6521 kcal/mol@LB@Unfolding Energy - sRNA 0.970016 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 32 -- 48@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 43 |
0.00194 |
0.1492 |
bsu01370 |
adk |
-12.74 |
0.079154 |
128 -- 149 |
32 -- 52 |
adenylate kinase |
|
_____________127____________________150\n_____________|______________________|\n5'-UUU...UACCG_______A___AAAAC______AAAUG...AUA-3'\n______________UGGAUUU_UGA_____UAGAGG\n______________:||||:|_|||_____||||||\n______________GCCUAGA_ACU_____AUCUCC\n3'-UUU...AAAAA___________AGACU______CCAAC...AUA-5'\n_____________|______________________|\n_____________53_____________________31\n |
128 -- 134 |
46 -- 52 |
-16.4639 kcal/mol |
2.75066 kcal/mol |
0.976384 kcal/mol |
127 150@LB@ | |@LB@5'-UUU...UACCG A AAAAC AAAUG...AUA-3'@LB@ UGGAUUU UGA UAGAGG@LB@ :||||:| ||| ||||||@LB@ GCCUAGA ACU AUCUCC@LB@3'-UUU...AAAAA AGACU CCAAC...AUA-5'@LB@ | |@LB@ 53 31@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -16.4639 kcal/mol@LB@Unfolding Energy - mRNA 2.75066 kcal/mol@LB@Unfolding Energy - sRNA 0.976384 kcal/mol@LB@@LB@Position - mRNA 128 -- 149@LB@Position - sRNA 32 -- 52@LB@Position Seed - mRNA 128 -- 134@LB@Position Seed - sRNA 46 -- 52@LB@ |
| 44 |
0.001968 |
0.1492 |
bsu14940 |
ylbA |
-14.48 |
0.038144 |
221 -- 240 |
24 -- 52 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________220___________________________241\n_____________|_____________________________|\n5'-CAU...GAAAG_________________________AA__CUGAG...AAU-3'\n______________CGGA_CUUGA________AGGGGUU__GG\n______________||||_|||||________|||||||__||\n______________GCCU_GAACU________UCCCCAA__CC\n3'-UUU...AAAAA____A_____AGACUAUC_______CC__UUGUC...AUA-5'\n_____________|_____________________________|\n_____________53____________________________23\n |
230 -- 236 |
28 -- 34 |
-22.4266 kcal/mol |
6.96942 kcal/mol |
0.980851 kcal/mol |
220 241@LB@ | |@LB@5'-CAU...GAAAG AA CUGAG...AAU-3'@LB@ CGGA CUUGA AGGGGUU GG@LB@ |||| ||||| ||||||| ||@LB@ GCCU GAACU UCCCCAA CC@LB@3'-UUU...AAAAA A AGACUAUC CC UUGUC...AUA-5'@LB@ | |@LB@ 53 23@LB@@LB@@LB@Energy -14.48 kcal/mol@LB@Hybridization Energy -22.4266 kcal/mol@LB@Unfolding Energy - mRNA 6.96942 kcal/mol@LB@Unfolding Energy - sRNA 0.980851 kcal/mol@LB@@LB@Position - mRNA 221 -- 240@LB@Position - sRNA 24 -- 52@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 45 |
0.002015 |
0.1494 |
bsu34040 |
yvfW |
-14.27 |
0.041807 |
34 -- 68 |
10 -- 49 |
lactate utilization protein B |
|
_____________33_______________________________________69\n_____________|________________________________________|\n5'-GCG...AAAAA___GA___G_________AAAC__________________GAUCG...GUC-3'\n______________GUC___AU_UG_UGGAGG_____GGGAGCAGAG_GU_GCU\n______________:||___||_||_|:||||_____||||:|||||_||_|||\n______________UAG___UA_AC_AUCUCC_____CCCUUGUCUC_CA_CGA\n3'-UUU...AAGCC___AAC__G__U______CCAAC__________U__U___AGAGA...AUA-5'\n_____________|________________________________________|\n_____________50_______________________________________9\n |
55 -- 61 |
19 -- 25 |
-34.7578 kcal/mol |
10.5343 kcal/mol |
9.9558 kcal/mol |
33 69@LB@ | |@LB@5'-GCG...AAAAA GA G AAAC GAUCG...GUC-3'@LB@ GUC AU UG UGGAGG GGGAGCAGAG GU GCU@LB@ :|| || || |:|||| ||||:||||| || |||@LB@ UAG UA AC AUCUCC CCCUUGUCUC CA CGA@LB@3'-UUU...AAGCC AAC G U CCAAC U U AGAGA...AUA-5'@LB@ | |@LB@ 50 9@LB@@LB@@LB@Energy -14.27 kcal/mol@LB@Hybridization Energy -34.7578 kcal/mol@LB@Unfolding Energy - mRNA 10.5343 kcal/mol@LB@Unfolding Energy - sRNA 9.9558 kcal/mol@LB@@LB@Position - mRNA 34 -- 68@LB@Position - sRNA 10 -- 49@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 46 |
0.002102 |
0.1496 |
bsu35160 |
uvrA |
-18.29 |
0.005742 |
210 -- 232 |
21 -- 44 |
UvrABC system protein A |
|
_____________209______________________233\n_____________|________________________|\n5'-CAC...CUAUG____G________UGAA_______CAGGG...GUU-3'\n______________GAUC_GAUAGAGG_____GGGAGC\n______________||||_||||||||_____||||:|\n______________CUAG_CUAUCUCC_____CCCUUG\n3'-UUU...UAGAA____A________CCAAC______UCUCU...AUA-5'\n_____________|________________________|\n_____________45_______________________20\n |
216 -- 222 |
32 -- 38 |
-26.4443 kcal/mol |
5.3846 kcal/mol |
2.7671 kcal/mol |
209 233@LB@ | |@LB@5'-CAC...CUAUG G UGAA CAGGG...GUU-3'@LB@ GAUC GAUAGAGG GGGAGC@LB@ |||| |||||||| ||||:|@LB@ CUAG CUAUCUCC CCCUUG@LB@3'-UUU...UAGAA A CCAAC UCUCU...AUA-5'@LB@ | |@LB@ 45 20@LB@@LB@@LB@Energy -18.29 kcal/mol@LB@Hybridization Energy -26.4443 kcal/mol@LB@Unfolding Energy - mRNA 5.3846 kcal/mol@LB@Unfolding Energy - sRNA 2.7671 kcal/mol@LB@@LB@Position - mRNA 210 -- 232@LB@Position - sRNA 21 -- 44@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 47 |
0.002107 |
0.1496 |
bsu10620 |
addB |
-16.79 |
0.012734 |
176 -- 222 |
12 -- 44 |
ATP-dependent helicase/deoxyribonuclease subunit B |
|
_____________175_____________________________________________223\n_____________|_______________________________________________|\n5'-UAG...AGAGA___AAAAAG________CUUCUAAUUU__________UUUUU_____GCAGG...AGC-3'\n______________GAU______AGAGGGGU__________GGGAGCAGAG_____AGUAG\n______________|||______||||||||__________||||:|||||_____|||||\n______________CUA______UCUCCCCA__________CCCUUGUCUC_____UCAUC\n3'-UUU...UAGAA___GACUA_________AC____________________________GAAGA...AUA-5'\n_____________|_______________________________________________|\n_____________45______________________________________________11\n |
206 -- 212 |
17 -- 23 |
-35.2405 kcal/mol |
8.50165 kcal/mol |
9.94637 kcal/mol |
175 223@LB@ | |@LB@5'-UAG...AGAGA AAAAAG CUUCUAAUUU UUUUU GCAGG...AGC-3'@LB@ GAU AGAGGGGU GGGAGCAGAG AGUAG@LB@ ||| |||||||| ||||:||||| |||||@LB@ CUA UCUCCCCA CCCUUGUCUC UCAUC@LB@3'-UUU...UAGAA GACUA AC GAAGA...AUA-5'@LB@ | |@LB@ 45 11@LB@@LB@@LB@Energy -16.79 kcal/mol@LB@Hybridization Energy -35.2405 kcal/mol@LB@Unfolding Energy - mRNA 8.50165 kcal/mol@LB@Unfolding Energy - sRNA 9.94637 kcal/mol@LB@@LB@Position - mRNA 176 -- 222@LB@Position - sRNA 12 -- 44@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 17 -- 23@LB@ |
| 48 |
0.002156 |
0.1498 |
bsu34050 |
yvfV |
-12.63 |
0.082536 |
177 -- 196 |
28 -- 46 |
lactate utilization protein A |
|
_____________176__________________197\n_____________|____________________|\n5'-UCG...AUAUA____GGA___G_________CAUCA...UUG-3'\n______________UUGG___UGA_AGAGGGGUU\n______________|||:___|||_|||||||||\n______________AACU___ACU_UCUCCCCAA\n3'-UUU...CCUAG____AG____A_________CCCCU...AUA-5'\n_____________|____________________|\n_____________47___________________27\n |
190 -- 196 |
28 -- 34 |
-21.1804 kcal/mol |
7.57881 kcal/mol |
0.97023 kcal/mol |
176 197@LB@ | |@LB@5'-UCG...AUAUA GGA G CAUCA...UUG-3'@LB@ UUGG UGA AGAGGGGUU@LB@ |||: ||| |||||||||@LB@ AACU ACU UCUCCCCAA@LB@3'-UUU...CCUAG AG A CCCCU...AUA-5'@LB@ | |@LB@ 47 27@LB@@LB@@LB@Energy -12.63 kcal/mol@LB@Hybridization Energy -21.1804 kcal/mol@LB@Unfolding Energy - mRNA 7.57881 kcal/mol@LB@Unfolding Energy - sRNA 0.97023 kcal/mol@LB@@LB@Position - mRNA 177 -- 196@LB@Position - sRNA 28 -- 46@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 49 |
0.002227 |
0.1516 |
bsu11839 |
yjzE |
-19.04 |
0.003744 |
170 -- 194 |
25 -- 51 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 11976315 |
|
_____________169_________________________195\n_____________|___________________________|\n5'-UCC...UUAAG___AAA___GACAGC____________UAUUG...GCU-3'\n______________GGA____GA______AGAGGGGU_GGG\n______________|||____||______||||||||_|||\n______________CCU____CU______UCUCCCCA_CCC\n3'-UUU...AAAAG___AGAA__AGACUA________A___CUUGU...AUA-5'\n_____________|___________________________|\n_____________52__________________________24\n |
185 -- 191 |
29 -- 35 |
-23.53 kcal/mol |
3.51134 kcal/mol |
0.976067 kcal/mol |
169 195@LB@ | |@LB@5'-UCC...UUAAG AAA GACAGC UAUUG...GCU-3'@LB@ GGA GA AGAGGGGU GGG@LB@ ||| || |||||||| |||@LB@ CCU CU UCUCCCCA CCC@LB@3'-UUU...AAAAG AGAA AGACUA A CUUGU...AUA-5'@LB@ | |@LB@ 52 24@LB@@LB@@LB@Energy -19.04 kcal/mol@LB@Hybridization Energy -23.53 kcal/mol@LB@Unfolding Energy - mRNA 3.51134 kcal/mol@LB@Unfolding Energy - sRNA 0.976067 kcal/mol@LB@@LB@Position - mRNA 170 -- 194@LB@Position - sRNA 25 -- 51@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 50 |
0.002285 |
0.1525 |
bsu22460 |
ypjH |
-13.49 |
0.058216 |
160 -- 194 |
23 -- 52 |
glycosyltransferase YpjH |
|
_____________159____________________________________195\n_____________|______________________________________|\n5'-AAG...CCAAA_____GCUGAUG_______A_____U__C_________CGAAC...AUG-3'\n______________CGGAU_______CUUGAUC_UGAUG__G___UUGGGGG\n______________|||||_______|||||||_||||:__|___||||||:\n______________GCCUA_______GAACUAG_ACUAU__C___AACCCCU\n3'-UUU...AAAAA_________________________CU_CCC_______UGUCU...AUA-5'\n_____________|______________________________________|\n_____________53_____________________________________22\n |
188 -- 194 |
23 -- 29 |
-25.8094 kcal/mol |
11.3359 kcal/mol |
0.981961 kcal/mol |
159 195@LB@ | |@LB@5'-AAG...CCAAA GCUGAUG A U C CGAAC...AUG-3'@LB@ CGGAU CUUGAUC UGAUG G UUGGGGG@LB@ ||||| ||||||| ||||: | ||||||:@LB@ GCCUA GAACUAG ACUAU C AACCCCU@LB@3'-UUU...AAAAA CU CCC UGUCU...AUA-5'@LB@ | |@LB@ 53 22@LB@@LB@@LB@Energy -13.49 kcal/mol@LB@Hybridization Energy -25.8094 kcal/mol@LB@Unfolding Energy - mRNA 11.3359 kcal/mol@LB@Unfolding Energy - sRNA 0.981961 kcal/mol@LB@@LB@Position - mRNA 160 -- 194@LB@Position - sRNA 23 -- 52@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 23 -- 29@LB@ |
